Retinoid-liposomes for treating fibrosis

ABSTRACT

What is described are pharmaceutical compositions comprising a double-stranded nucleic acid molecule comprising a sense strand and an antisense strand wherein the sense and antisense strands are selected from the oligonucleotides described as SERPINH1_2 (SEQ ID NOS: 60 and 127), SERPINH1_45a (SEQ ID NOS: 98 and 165), and SERPINH1_51 (SEQ ID NOS: 101 and 168), and drug carrier comprising a mixture of a retinoid and a lipid vesicle, and methods of using these pharmaceutical compositions to treat a disease associated with hsp47 espresssion, including fibrosis.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is a continuation of patent application Ser. No. 13/492,594 filed on Jun. 8, 2012, which claims benefit under 35 U.S.C. §119(e) of Provisional U.S., patent application no. 61/494,832 filed on Jun. 8, 2011, the entire contents of which are incorporated herein by reference in their entirety.

SEQUENCE LISTING

The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Aug. 8, 2014, is named 101025.000051_SL.txt and is 663,519 bytes in size.

TECHNICAL FIELD

Provided herein are pharmaceutical compositions comprising retinoid-liposomes for enhancing the modulation of hsp47 expression by siRNA.

BACKGROUND

Fibrosis of the liver can be caused by activated hepatic stellate cells (HSC), resulting in a plurality of types of collagen molecules and fibronectin being deposited on interstitial tissue. This can lead to hepatic cirrhosis, hepatic failure, and/or hepatocellular carcinoma. Further, chronic pancreatitis develops as a result of pancreatic fibrosis by the same mechanism as that for hepatic fibrosis (Madro, et al., 2004; Med Sci Monit. 10:RA166-70; Jaster, 2004, Mol Cancer. 6:26). Furthermore, stellate cells are present in disorders of the vocal cord and larynx such as vocal cord scarring, vocal cord mucosal fibrosis, and laryngeal fibrosis. To prevent or treat fibrosis in these organs and elsewhere in the body, there is a desire for the development of a drug carrier and drug carrier kit.

Stellate cells are one of the important target candidates for treating fibrosis (Fallowfield et al., 2004, Expert Opin Ther Targets. 8:423-35; Pinzani, et al., 2004, Dig Liver Dis. 36:231-42). During fibrosis, stellate cells are activated by cytokines from nearby cells to produce many factors that cause hepatic fibrosis. Stellate cells store vitamin A, and belong to the myofibroblast family.

Therapeutic methods to prevent or treat fibrosis attempt to control collagen metabolism, promotion of the collagen degradation system, and inhibition of activation of stellate cells. However, in all these cases, the low specificity of action and/or the low organ specificity, limited efficacy and adverse side effects create problems.

Inhibition of collagen protein synthesis has not been established as a therapeutic method. The potency of molecules targeting collagen production is limited due to the possibility of causing side effects. Inhibiting collagen production directly provides another therapeutic method to prevent or treat fibrosis. Such a method requires controlling one or more of the various types of collagen Types I to IV. A method for accomplishing this may be through heat shock protein47 (HSP47), a collagen-specific molecular chaperone that is essential for intracellular transport and molecular maturation necessary for various types of collagen. Therefore, if the function of HSP47 can be specifically controlled in stellate cells, there is a possibility of inhibiting hepatic fibrosis.

SUMMARY

The present description relates to a drug carrier and a drug carrier kit that enable a diagnostic and/or therapeutic drug to be specifically transported to stellate cells. The drug carrier in the present description may be selected from polymer micelles, liposomes, emulsions, microspheres, and nanosphere forms, and by bonding thereto or including therein a retinoid or retinoid conjugate and a therapeutic drug that can be transported specifically to HSC. Retinoids include vitamin A, retinal, retinoic acid, saturated Vitamin A, tretinoin, adapalene, or retinol palmitate, and fenretinide (4-HPR). Furthermore, by preparing the drug carrier to include one molecule or a plurality of molecules selected from TGFβ activity inhibitors such as a truncated TGFβ type II receptor and a soluble TGFβ type II receptor, growth factor preparations such as HGF, MMP production promoters such as an MMP gene-containing adenovirus vector, a cell activation inhibitors and/or growth inhibitors including a PPARγ-ligand, an angiotensin-II type I receptor antagonist, a PDGF tyrosine kinase inhibitor, and a sodium channel inhibitor such as amiloride, and apoptosis inducers such as compound 861 and gliotoxin; and by administering it for example, orally, parenterally, intravenously or intraperitoneally to a patient having a risk of fibrosis or fibrosis symptoms, or patients having various fibrosis-related disorders such as, for example, hepatic cirrhosis, hepatic failure, liver cancer, or chronic pancreatitis, the activation of stellate cells can be suppressed, and thereby preventing, inhibiting or improving the fibrosis and/or fibrosis-related disease conditions in said patient. Alternatively, or in addition thereto, by using the drug carrier which encloses therein a ribozyme, an antisense RNA, or an siRNA that specifically inhibits HSP47 or TIMP, which is an MMP inhibitor, secretion of type I to IV collagens can be simultaneously inhibited, and as a result fibrogenesis can be inhibited effectively.

An embodiment of the description is a pharmaceutical composition comprising a double-stranded nucleic acid molecule comprising a sense strand and an antisense strand wherein the sense and antisense strands are selected from the oligonucleotides described as SERPINH1_(—)2 (SEQ ID NOS: 60 and 127), SERPINH1_(—)45a (SEQ ID NOS: 98 and 165), and SERPINH1_(—)51 (SEQ ID NOS: 101 and 168) in Table 4, infra, and a drug carrier comprising a mixture of a lipid vesicle and a retinoid or a retinoid conjugate. The retinoid can be one or more of the following: vitamin A, retinoic acid, saturated Vitamin A, retinal, tretinoin, adapalene, retinol palmitate, or fenretinide. Preferably, the retinoid comprises a conjugate of retinoic acid, most preferably a retinoid-PEG conjugate. The lipid vesicle can be comprised of a bilayer of lipid molecules, and can further be comprised of the retinoid. The retinoid is preferably at a concentration of 0.2 to 20 wt % in the drug carrier. The lipid vesicle can be comprised of an interior surface that encapsulates the interior of the lipid vesicle, and an exterior surface that is accessible to an aqueous medium outside of the lipid vesicle. The retinoid may be associated with the lipid bilayer. The double-stranded nucleic acid can be exposed on the exterior surface of the lipid vesicle.

In some embodiments, the double-stranded nucleic acid molecule includes an antisense strand having SEQ ID NO:127 and comprising 2′-O-methyl sugar (2′OMe)-modified ribonucleotides; a 2′-5′-ribonucleotide in at least one of positions 1, 5, 6, or 7; and a 3′-terminal non-nucleotide moiety covalently attached to the 3′-terminus; and a sense strand having SEQ ID NO:60 and comprising at least one 2′-5′-ribonucleotide or 2′OMe modified ribonucleotide; a non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic moiety covalently attached at the 5′-terminus. In preferred embodiments the antisense strand is SEQ ID NO:127 and comprises 2′OMe modified ribonucleotides at positions 3, 5, 9, 11, 13, 15, 17, and 19; a 2′-5′-ribonucleotide in position 7; and a non-nucleotide moiety covalently attached at the 3′-terminus; and the sense strand is SEQ ID NO:60 and comprises five consecutive 2′-5′-ribonucleotides in the 3′-terminal positions 15, 16, 17, 18, and 19; a non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic moiety covalently attached at the 5′-terminus. In some embodiments the double-stranded nucleic acid molecule further includes a 2′OMe modified ribonucleotide or a 2′-5′-ribonucleotide at position 1 of the antisense strand. (All reference herein to nucleotide positions are expressed based on the 5′>3′ direction of the oligonucleotide for both sense and antisense strands of the double-stranded nucleic acid molecule.)

In various embodiments, the sense strand is SEQ ID NO:98 and comprises 2′-5′-ribonucleotides in positions at the 3′-terminus; a non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic moiety covalently attached at the 5′-terminus; and the antisense strand is SEQ ID NO:165 and comprises 2′OMe modified ribonucleotides; a 2′-5′-ribonucleotide in at least one of positions 5, 6 or 7; and a non-nucleotide moiety covalently attached at the 3′-terminus. In preferred embodiments the sense strand is SEQ ID NO:98 and comprises 2′-5′-ribonucleotides in positions 15, 16, 17, 18, and 19; a C3-OH 3′ moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand is SEQ ID NO:165 and comprises 2′OMe modified ribonucleotides in positions 4, 6, 8, 11, 13, 15, 17, and 19; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH moiety covalently attached at the 3′-terminus. In some embodiments the double-stranded nucleic acid further comprises a 2′OMe modified ribonucleotide in position 2.

In various embodiments, the sense strand is SEQ ID NO:101 and comprises 2′OMe modified ribonucleotides; an optional 2′-5′-ribonucleotide in one of position 9 or 10; a non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic moiety covalently attached at the 5′-terminus; and the antisense strand is SEQ ID NO:168 and comprises 2′OMe modified ribonucleotides; a 2′-5′-ribonucleotide in at least one of positions 5, 6, or 7; and a non-nucleotide moiety covalently attached at the 3′-terminus. In preferred embodiments the sense strand is SEQ ID NO:101 and comprises 2′OMe modified ribonucleotides in positions 4, 11, 13, and 17; a 2′-5′-ribonucleotide in position 9; a C3OH non-nucleotide moiety covalently attached at the 3′-terminus; and inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand is SEQ ID NO:168 and comprises 2′OMe modified ribonucleotides in positions 1, 4, 8, 11 and 15; a 2′-5′-ribonucleotide in position 6; and a C3Pi-C3OH moiety covalently attached at the 3′-terminus. In certain embodiments the double-stranded nucleic acid molecule further comprises a 2′OMe modified ribonucleotide in position 13 in the antisense strand and or in position 2 in the sense strand.

Another aspect is a pharmaceutical composition comprising a double-stranded nucleic acid molecule and drug carrier comprising a mixture of a retinoid and a lipid, wherein the double-stranded oligonucleotide compound comprising the structure (A1):

(A1) 5′ (N)_(x)-Z      3′ (antisense strand) 3′ Z′-(N′)_(y)-z″ 5′ (sense strand) wherein each of N and N′ is a nucleotide which may be unmodified or modified, or an unconventional moiety; wherein each of (N)_(x) and (N′)_(y) is an oligonucleotide in which each consecutive N or N′ is joined to the next N or N′ by a covalent bond; wherein each of Z and Z′ is independently present or absent, but if present independently includes 1-5 consecutive nucleotides or non-nucleotide moieties or a combination thereof covalently attached at the 3′-terminus of the strand in which it is present; wherein z″ may be present or absent, but if present is a capping moiety covalently attached at the 5′-terminus of (N′)_(y); wherein each of x and y is independently an integer between 18 and 40; wherein the sequence of (N′)_(y) has complementary to the sequence of (N)_(x); and wherein (N)_(x) includes an antisense sequence to the mRNA coding sequence for human hsp47 exemplified by SEQ ID NO:1, which is shown as follows.

ucuuuggcuu uuuuuggcgg agcuggggcg cccuccggaa gcguuuccaa cuuuccagaa 60 guuucucggg acgggcagga gggggugggg acugccauau auagaucccg ggagcagggg 120 agcgggcuaa gaguagaauc gugucgcggc ucgagagcga gagucacguc ccggcgcuag 180 cccagcccga cccaggccca ccguggugca cgcaaaccac uuccuggcca ugcgcucccu 240 ccugcuucuc agcgccuucu gccuccugga ggcggcccug gccgccgagg ugaagaaacc 300 ugcagccgca gcagcuccug gcacugcgga gaaguugagc cccaaggcgg ccacgcuugc 360 cgagcgcagc gccggccugg ccuucagcuu guaccaggcc auggccaagg accaggcagu 420 ggagaacauc cuggugucac ccgugguggu ggccucgucg cuagggcucg ugucgcuggg 480 cggcaaggcg accacggcgu cgcaggccaa ggcagugcug agcgccgagc agcugcgcga 540 cgaggaggug cacgccggcc ugggcgagcu gcugcgcuca cucagcaacu ccacggcgcg 600 caacgugacc uggaagcugg gcagccgacu guacggaccc agcucaguga gcuucgcuga 660 ugacuucgug cgcagcagca agcagcacua caacugcgag cacuccaaga ucaacuuccg 720 cgacaagcgc agcgcgcugc aguccaucaa cgagugggcc gcgcagacca ccgacggcaa 780 gcugcccgag gucaccaagg acguggagcg cacggacggc gcccugcuag ucaacgccau 840 guucuucaag ccacacuggg augagaaauu ccaccacaag augguggaca accguggcuu 900 cauggugacu cgguccuaua ccgugggugu caugaugaug caccggacag gccucuacaa 960 cuacuacgac gacgagaagg aaaagcugca aaucguggag augccccugg cccacaagcu 1020 cuccagccuc aucauccuca ugccccauca cguggagccu cucgagcgcc uugaaaagcu 1080 gcuaaccaaa gagcagcuga agaucuggau ggggaagaug cagaagaagg cuguugccau 1140 cuccuugccc aagggugugg uggaggugac ccaugaccug cagaaacacc uggcugggcu 1200 gggccugacu gaggccauug acaagaacaa ggccgacuug ucacgcaugu caggcaagaa 1260 ggaccuguac cuggccagcg uguuccacgc caccgccuuu gaguuggaca cagauggcaa 1320 ccccuuugac caggacaucu acgggcgcga ggagcugcgc agccccaagc uguucuacgc 1380 cgaccacccc uucaucuucc uagugcggga cacccaaagc ggcucccugc uauucauugg 1440 gcgccugguc cggccuaagg gugacaagau gcgagacgag uuauagggcc ucagggugca 1500 cacaggaugg caggaggcau ccaaaggcuc cugagacaca ugggugcuau ugggguuggg 1560 ggggagguga gguaccagcc uuggauacuc cauggggugg ggguggaaaa acagaccggg 1620 guucccgugu gccugagcgg accuucccag cuagaauuca cuccacuugg acaugggccc 1680 cagauaccau gaugcugagc ccggaaacuc cacauccugu gggaccuggg ccauagucau 1740 ucugccugcc cugaaagucc cagaucaagc cugccucaau caguauucau auuuauagcc 1800 agguaccuuc ucaccuguga gaccaaauug agcuaggggg gucagccagc ccucuucuga 1860 cacuaaaaca ccucagcugc cuccccagcu cuaucccaac cucucccaac uauaaaacua 1920 ggugcugcag ccccugggac caggcacccc cagaaugacc uggccgcagu gaggcggauu 1980 gagaaggagc ucccaggagg ggcuucuggg cagacucugg ucaagaagca ucgugucugg 2040 cguugugggg augaacuuuu uguuuuguuu cuuccuuuuu uaguucuuca aagauaggga 2100 gggaaggggg aacaugagcc uuuguugcua ucaauccaag aacuuauuug uacauuuuuu 2160 uuuucaauaa aacuuuucca augacauuuu guuggagcgu ggaaaaaa 2208

Compositions, methods and kits for modulating expression of target genes are provided herein. In various aspects and embodiments, compositions, methods and kits provided herein modulate expression of heat shock protein 47 (hsp47), also known as SERPINH1 (SEQ ID NO:1). The compositions, methods and kits may involve use of nucleic acid molecules (for example, short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), or short hairpin RNA (shRNA)) that bind a nucleotide sequence (such as an mRNA sequence) encoding hsp47, SEQ ID NO:1. In certain preferred embodiments, the compositions, methods and kits disclosed herein inhibit expression of hsp47. For example, siNA molecules (e.g., RNA-induced silencing complex (RISC) length dsNA molecules or Dicer length dsNA molecules) are provided that reduce or inhibit hsp47 expression. Also provided are compositions, methods and kits for treating and/or preventing diseases, conditions or disorders associated with hsp47, such as liver fibrosis, cirrhosis, pulmonary fibrosis including lung fibrosis (including interstitial lung fibrosis (ILF)), kidney fibrosis resulting from any condition (e.g., chronic kidney disease (CKD) including End-Stage Renal Disease (ESRD)), peritoneal fibrosis, chronic hepatic damage, fibrillogenesis, fibrotic diseases in other organs, abnormal scarring (keloids) associated with all possible types of skin injury accidental and jatrogenic (operations); scleroderma; cardiofibrosis, failure of glaucoma filtering operation; and intestinal adhesions.

In one aspect, provided are the pharmaceutical compositions, above, comprising nucleic acid molecules (e.g., siNA molecules) as a component of a pharmaceutical formulation in which the nucleic acid molecule includes a sense strand and an antisense strand; each strand of the nucleic acid molecule is independently 15 to 49 nucleotides in length; a 15 to 49 nucleotide sequence of the antisense strand is complementary to a sequence of an mRNA encoding human hsp47 (e.g., SEQ ID NO: 1); and a 15 to 49 nucleotide sequence of the sense strand is complementary to the a sequence of the antisense strand and includes a 15 to 49 nucleotide sequence of an mRNA encoding human hsp47 (e.g., SEQ ID NO: 1).

In certain embodiments, the sequence of the antisense strand that is complementary to a sequence of an mRNA encoding human hsp47 includes a sequence complimentary to a sequence between nucleotides 600-800; or 801-899; or 900-1000; or 1001-1300 of SEQ ID NO: 1; or between nucleotides 650-730; or 900-975 of SEQ ID NO: 1. In some embodiments, the antisense strand includes a sequence that is complementary to a sequence of an mRNA encoding human hsp47 corresponding to nucleotides 674-693 of SEQ ID NO: 1 or a portion thereof; or nucleotides 698-716 of SEQ ID NO: 1 or a portion thereof; or nucleotides 698-722 of SEQ ID NO: 1 or a portion thereof; or nucleotides 701-720 of SEQ ID NO: 1 or a portion thereof; or nucleotides 920-939 of SEQ ID NO: 1 or a portion thereof; or nucleotides 963-982 of SEQ ID NO: 1 or a portion thereof; or nucleotides 947-972 of SEQ ID NO: 1 or a portion thereof; or nucleotides 948-966 of SEQ ID NO: 1 or a portion thereof; or nucleotides 945-969 of SEQ ID NO: 1 or a portion thereof; or nucleotides 945-963 of SEQ ID NO: 1 or a portion thereof

In certain embodiments, the antisense strand of a nucleic acid molecule (e.g., a siNA molecule) as disclosed herein as a component of a pharmaceutical formulation includes a sequence corresponding to SEQ ID NO: 4 or a portion thereof; or SEQ ID NO: 6 or a portion thereof; or SEQ ID NO: 8 or a portion thereof; or SEQ ID NO: 10 or a portion thereof; or SEQ ID NO: 12 or a portion thereof; or SEQ ID NO: 14 or a portion thereof; or SEQ ID NO: 16 or a portion thereof; or SEQ ID NO: 18 or a portion thereof; or SEQ ID NO: 20 or a portion thereof; or SEQ ID NO: 22 or a portion thereof; or SEQ ID NO: 24 or a portion thereof; or SEQ ID NO: 26 or a portion thereof; or SEQ ID NO: 28 or a portion thereof; or SEQ ID NO: 30 or a portion thereof; or SEQ ID NO: 32 or a portion thereof; or SEQ ID NO: 34 or a portion thereof; or SEQ ID NO: 36 or a portion thereof; or SEQ ID NO: 38 or a portion thereof; or SEQ ID NO: 40 or a portion thereof; or SEQ ID NO: 42 or a portion thereof; or SEQ ID NO: 44 or a portion thereof; or SEQ ID NO: 46 or a portion thereof; or SEQ ID NO: 48 or a portion thereof; or SEQ ID NO: 50 or a portion thereof; or SEQ ID NO: 52 or a portion thereof; or SEQ ID NO: 54 or a portion thereof; or SEQ ID NO: 56 or a portion thereof; or SEQ ID NO: 58 or a portion thereof. In certain embodiments, the sense strand of a nucleic acid molecule (e.g., a siNA molecule) as disclosed herein includes a sequence corresponding to SEQ ID NO: 3 or a portion thereof; or SEQ ID NO: 5 or a portion thereof; or SEQ ID NO: 7 or a portion thereof; or SEQ ID NO: 9 or a portion thereof; or SEQ ID NO: 11 or a portion thereof; or SEQ ID NO: 13 or a portion thereof; or SEQ ID NO: 15 or a portion thereof; or SEQ ID NO: 17 or a portion thereof; or SEQ ID NO: 19 or a portion thereof; or SEQ ID NO: 21 or a portion thereof; or SEQ ID NO: 23 or a portion thereof; or SEQ ID NO: 25 or a portion thereof; or SEQ ID NO: 27 or a portion thereof; or SEQ ID NO: 29 or a portion thereof; or SEQ ID NO: 31 or a portion thereof; or SEQ ID NO: 33 or a portion thereof; or SEQ ID NO: 35 or a portion thereof; or SEQ ID NO: 37 or a portion thereof; or SEQ ID NO: 39 or a portion thereof; or SEQ ID NO: 41 or a portion thereof; or SEQ ID NO: 43 or a portion thereof; or SEQ ID NO: 45 or a portion thereof; or SEQ ID NO: 47 or a portion thereof; or SEQ ID NO: 49 or a portion thereof; or SEQ ID NO: 51 or a portion thereof; or SEQ ID NO: 53 or a portion thereof; or SEQ ID NO: 55 or a portion thereof; or SEQ ID NO: 57 or a portion thereof

In certain preferred embodiments, the antisense strand of a nucleic acid molecule (e.g., a siNA molecule) as disclosed herein as a component of a pharmaceutical formulation includes a sequence corresponding to any one of the antisense sequences shown on Table 4. In certain preferred embodiments the antisense strand and the strand are selected from the sequence pairs shown in Table 4. In some embodiments the antisense and sense strands are selected from the sequence pairs set forth in SERPINH1_(—)4, SERPINH1_(—)12, SERPINH1_(—)18, SERPINH1_(—)30, SERPINH1_(—)58 and SERPINH1_(—)88. In some embodiments the antisense and sense strands are selected from the sequence pairs set forth in SERPINH1_(—)4 (SEQ ID NOS:195 and 220), SERPINH1_(—)12 (SEQ ID NOS:196 and 221), SERPINH1_(—)30 (SEQ ID NOS:199 and 224), and SERPINH1_(—)58 (SEQ ID NOS:208 and 233).

In some embodiments, the antisense and sense strands of a nucleic acid molecule (e.g., a siNA molecule) as disclosed herein as a component of a pharmaceutical formulation includes the sequence pairs set forth in SERPINH1_(—)4 (SEQ ID NOS:195 and 220). In some embodiments of a nucleic acid molecule (e.g., a siNA molecule) as disclosed herein includes the antisense and sense strands of the sequence pairs set forth in SERPINH1_(—)12 (SEQ ID NOS:196 and 221). In some embodiments the antisense and sense strands of a nucleic acid molecule (e.g., a siNA molecule) as disclosed herein includes the sequence pairs set forth in SERPINH1_(—)30 (SEQ ID NOS:199 and 224). In some embodiments of a nucleic acid molecule (e.g., a siNA molecule) as disclosed herein includes the antisense and sense strands of the sequence pairs set forth in SERPINH1_(—)58 (SEQ ID NOS:208 and 233).

In certain embodiments, the antisense strand of a nucleic acid molecule (e.g., a siNA molecule) as disclosed herein as a component of a pharmaceutical formulation includes a sequence corresponding to any one of the antisense sequences shown on any one of Tables B or C.

In certain preferred embodiments, the antisense strand of a nucleic acid molecule (e.g., a siNA molecule) as disclosed herein as a component of a pharmaceutical formulation includes a sequence corresponding to any one of the antisense sequences shown on Table 5. In certain preferred embodiments the antisense strand and the strand are selected from the sequence pairs shown in Table 5. In some embodiments of a nucleic acid molecule (e.g., a siNA molecule) as disclosed herein includes the antisense and sense strands selected from the sequence pairs set forth in

-   -   SERPINH1_(—)2 (SEQ ID NOS: 60 and 127),     -   SERPINH1_(—)6 (SEQ ID NOS: 63 and 130),     -   SERPINH1_(—)11 (SEQ ID NOS: 68 and 135),     -   SERPINH1_(—)13 (SEQ ID NOS: 69 and 136),     -   SERPINH1_(—)45 (SEQ ID NOS: 97 and 164),     -   SERPINH1_(—)45a (SEQ ID NOS: 98 and 165),     -   SERPINH1_(—)51 (SEQ ID NOS: 101 and 168),     -   SERPINH1_(—)52 (SEQ ID NOS:102 and 169) or     -   SERPINH1_(—)86 (SEQ ID NOS: 123 and 190).

In some preferred embodiments the antisense and sense strands are selected from the sequence pairs set forth in

-   -   SERPINH1_(—)2 (SEQ ID NOS: 60 and 127),     -   SERPINH1_(—)6 (SEQ ID NOS: 63 and 130),     -   SERPINH1_(—)45a (SEQ ID NOS: 98 and 165), and     -   SERPINH1_(—)51 (SEQ ID NOS: 101 and 168).

In some preferred embodiments of a nucleic acid molecule (e.g., a siNA molecule) as disclosed herein as a component of a pharmaceutical formulation includes the antisense and sense strands selected from the sequence pairs set forth in SERPINH1_(—)2 (SEQ ID NOS: 60 and 127). In some embodiments the antisense and sense strands include the sequence pairs set forth in SERPINH1_(—)6 (SEQ ID NOS: 63 and 130). In some embodiments of a nucleic acid molecule (e.g., a siNA molecule) as disclosed herein includes the antisense and sense strands of the sequence pairs set forth in SERPINH1_(—)11 (SEQ ID NOS: 68 and 135). In some embodiments the antisense and sense strands are the sequence pairs set forth in SERPINH1_(—)13 (SEQ ID NOS: 69 and 136). In some embodiments the antisense and sense strands are the sequence pairs set forth in SERPINH1_(—)45 (SEQ ID NOS: 97 and 164). In some embodiments the antisense and sense strands are the sequence pairs set forth in SERPINH1_(—)45a (SEQ ID NOS: 98 and 165). In some embodiments the antisense and sense strands are the sequence pairs set forth in SERPINH1_(—)51 (SEQ ID NOS: 101 and 168).

In certain embodiments, the antisense strand of a nucleic acid molecule (e.g., a siNA molecule) as disclosed as a component of a pharmaceutical formulation herein includes a sequence corresponding to any one of the antisense sequences shown on any one of Tables D or E.

In various embodiments of nucleic acid molecules (e.g., siNA molecules) as disclosed herein as a component of a pharmaceutical formulation, the antisense strand may be 15 to 49 nucleotides in length (e.g., 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48 or 49 nucleotides in length); or 17-35 nucleotides in length; or 17-30 nucleotides in length; or 15-25 nucleotides in length; or 18-25 nucleotides in length; or 18-23 nucleotides in length; or 19-21 nucleotides in length; or 25-30 nucleotides in length; or 26-28 nucleotides in length. In some embodiments of nucleic acid molecules (e.g., siNA molecules) as disclosed herein, the antisense strand may be 19 nucleotides in length. Similarly the sense strand of nucleic acid molecules (e.g., siNA molecules) as disclosed herein may be 15 to 49 nucleotides in length (e.g., 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48 or 49 nucleotides in length); or 17-35 nucleotides in length; or 17-30 nucleotides in length; or 15-25 nucleotides in length; or 18-25 nucleotides in length; or 18-23 nucleotides in length; or 19-21 nucleotides in length; or 25-30 nucleotides in length; or 26-28 nucleotides in length. In some embodiments of nucleic acid molecules (e.g., siNA molecules) as disclosed herein, the sense strand may be 19 nucleotides in length. In some embodiments of nucleic acid molecules (e.g., siNA molecules) as disclosed herein, the antisense strand and the sense strand may be 19 nucleotides in length. The duplex region of the nucleic acid molecules (e.g., siNA molecules) as disclosed herein may be 15-49 nucleotides in length (e.g., about 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48 or 49 nucleotides in length), 15-35 nucleotides in length; or 15-30 nucleotides in length; or about 15-25 nucleotides in length; or 17-25 nucleotides in length; or 17-23 nucleotides in length; or 17-21 nucleotides in length; or 25-30 nucleotides in length; or 25-28 nucleotides in length. In various embodiments of nucleic acid molecules (e.g., siNA molecules) as disclosed herein, the duplex region may be 19 nucleotides in length.

In certain embodiments, the sense and antisense strands of a nucleic acid (e.g., an siNA nucleic acid molecule) as provided herein as a component of a pharmaceutical formulation are separate polynucleotide strands. In some embodiments, the separate antisense and sense strands form a double-stranded structure via hydrogen bonding, for example, Watson-Crick base pairing. In some embodiments the sense and antisense strands are two separate strands that are covalently linked to each other. In other embodiments, the sense and antisense strands are part of a single polynucleotide strand having both a sense and antisense region; in some preferred embodiments the polynucleotide strand has a hairpin structure.

In certain embodiments, the nucleic acid molecule (e.g., siNA molecule) is a double-stranded nucleic acid (dsNA) molecule that is symmetrical with regard to overhangs, and has a blunt end on both ends. In other embodiments, the nucleic acid molecule (e.g., siNA molecule) is a dsNA molecule that is symmetrical with regard to overhangs, and has an overhang on both ends of the dsNA molecule; preferably the molecule has overhangs of 1, 2, 3, 4, 5, 6, 7, or 8 nucleotides; preferably the molecule has 2 nucleotide overhangs. In some embodiments, the overhangs are 5′ overhangs; in alternative embodiments the overhangs are 3′ overhangs. In certain embodiments, the overhang nucleotides are modified with modifications as disclosed herein. In some embodiments, the overhang nucleotides are 2′-deoxynucleotides.

In certain preferred embodiments, the nucleic acid molecule (e.g., siNA molecule) as a component of a pharmaceutical formulation is a dsNA molecule that is asymmetrical with regard to overhangs, and has a blunt end on one end of the molecule and an overhang on the other end of the molecule. In certain embodiments, the overhang is 1, 2, 3, 4, 5, 6, 7, or 8 nucleotides; preferably the overhang is 2 nucleotides. In some preferred embodiments, an asymmetrical dsNA molecule has a 3′-overhang (for example a two nucleotide 3′-overhang) on one side of a duplex occurring on the sense strand; and a blunt end on the other side of the molecule. In some preferred embodiments, an asymmetrical dsNA molecule has a 5′-overhang (for example a two nucleotide 5′-overhang) on one side of a duplex occurring on the sense strand; and a blunt end on the other side of the molecule. In other preferred embodiments, an asymmetrical dsNA molecule has a 3′-overhang (for example a two nucleotide 3′-overhang) on one side of a duplex occurring on the antisense strand; and a blunt end on the other side of the molecule. In some preferred embodiments, an asymmetrical dsNA molecule has a 5′-overhang (for example a two nucleotide 5′-overhang) on one side of a duplex occurring on the antisense strand; and a blunt end on the other side of the molecule. In certain preferred embodiments, the overhangs are 2′-deoxynucleotides.

In some embodiments, the nucleic acid molecule (e.g., siNA molecule) as a component of a pharmaceutical formulation has a hairpin structure (having the sense strand and antisense strand on one polynucleotide), with a loop structure on one end and a blunt end on the other end. In some embodiments, the nucleic acid molecule has a hairpin structure, with a loop structure on one end and an overhang end on the other end (for example a 1, 2, 3, 4, 5, 6, 7, or 8 nucleotide overhang); in certain embodiments, the overhang is a 3′-overhang; in certain embodiments the overhang is a 5′-overhang; in certain embodiments the overhang is on the sense strand; in certain embodiments the overhang is on the antisense strand.

In some preferred embodiments, the nucleic acid molecule is selected from the nucleic acid molecules shown on Table 3.

The nucleic acid molecules (e.g., siNA molecule) disclosed herein as a component of a pharmaceutical formulation may include one or more modifications or modified nucleotides such as described herein. For example, a nucleic acid molecule (e.g., siNA molecule) as provided herein may include a modified nucleotide having a modified sugar; a modified nucleotide having a modified nucleobase; or a modified nucleotide having a modified phosphate group. Similarly, a nucleic acid molecule (e.g., siNA molecule) as provided herein may include a modified phosphodiester backbone and/or may include a modified terminal phosphate group.

Nucleic acid molecules (e.g., siNA molecules) as provided as a component of a pharmaceutical formulation may have one or more nucleotides that include a modified sugar moiety, for example as described herein. In some preferred embodiments, the modified sugar moiety is selected from the group consisting of 2′OMe, 2′-methoxyethoxy, 2′-deoxy, 2′-fluoro, 2′-allyl, 2′-O-(2-(methylamino)-2-oxoethyl), 4′-thio, 4′-(CH₂)₂—O-2′-bridge, 2′-LNA (the ribose moiety of an LNA nucleotide is modified with an extra bridge connecting the 2′ oxygen and 4′ carbon), and 2′-O—(N-methylcarbamate).

Nucleic acid molecules (e.g., siNA molecules) as provided as a component of a pharmaceutical formulation may have one or more modified nucleobase(s) for example as described herein, which preferably may be one selected from the group consisting of xanthine, hypoxanthine, 2-aminoadenine, 6-methyl and other alkyl derivatives of adenine and guanine, 2-propyl and other alkyl derivatives of adenine and guanine, 5-halo uracil and cytosine, 5-propynyl uracil and cytosine, 6-azo uracil, cytosine and thymine, 5-uracil (pseudouracil), 4-thiouracil, 8-halo, amino, thiol, thioalkyl, hydroxyl and other 8-substituted adenines and guanines, 5-trifluoromethyl and other 5-substituted uracils and cytosines, 7-methylguanine, and acyclonucleotides.

Nucleic acid molecules (e.g., siNA molecules) as provided as a component of a pharmaceutical formulation may have one or more modifications to the phosphodiester backbone, for example as described herein. In some preferred embodiments, the phosphodiester bond is modified by substituting the phosphodiester bond with a phosphorothioate, 3′-(or 5′-) deoxy-3′-(or 5′-)thio-phosphorothioate, phosphorodithioate, phosphoroselenates, 3′-(or -5′)deoxy phosphinates, borano phosphates, 3′-(or 5′-)deoxy-3′-(or 5′-)amino phosphoramidates, hydrogen phosphonates, borano phosphate esters, phosphoramidates, alkyl or aryl phosphonates and phosphotriester or phosphorus linkages.

In various embodiments, the provided nucleic acid molecules (e.g., siNA molecules) as a component of a pharmaceutical formulation may include one or modifications in the sense strand but not the antisense strand. In some embodiments the provided nucleic acid molecules (e.g., siNA molecules) include one or more modifications in the antisense strand but not the sense strand. In some embodiments the provided nucleic acid molecules (e.g., siNA molecules) include one or more modifications in the both the sense strand and the antisense strand.

In some embodiments in which the provided nucleic acid molecules (e.g., siNA molecules) as a component of a pharmaceutical formulation have modifications, the sense strand includes a pattern of alternating modified and unmodified nucleotides, and/or the antisense strand includes a pattern of alternating modified and unmodified nucleotides; in some preferred versions of such embodiments, the modification is a 2′OMe moiety. The pattern of alternating modified and unmodified nucleotides may start with a modified nucleotide at the 5′-end or 3′-end of one of the strands; for example the pattern of alternating modified and unmodified nucleotides may start with a modified nucleotide at the 5′-end or 3′-end of the sense strand and/or the pattern of alternating modified and unmodified nucleotides may start with a modified nucleotide at the 5′-end or 3′-end of the antisense strand. When both the antisense and sense strand include a pattern of alternating modified nucleotides, the pattern of modified nucleotides may be configured such that modified nucleotides in the sense strand are opposite modified nucleotides in the antisense strand; or there may be a phase shift in the pattern such that modified nucleotides of the sense strand are opposite unmodified nucleotides in the antisense strand and vice-versa.

The nucleic acid molecules (e.g., siNA molecules) as provided herein as a component of a pharmaceutical formulation may include one to three (i.e., 1, 2 or 3) deoxynucleotides at the 3′-end of the sense and/or antisense strand.

The nucleic acid molecules (e.g., siNA molecules) as provided herein as a component of a pharmaceutical formulation may include a phosphate group at the 5′-end of the sense and/or antisense strand.

In one aspect, provided as a component of a pharmaceutical formulation are double-stranded nucleic acid molecules having the structure (A1):

(A1) 5′ (N)_(x)-Z      3′ (antisense strand) 3′ Z′-(N′)_(y)-z″ 5′ (sense strand) wherein each of N and N′ is a nucleotide which may be unmodified or modified, or an unconventional moiety; wherein each of (N)_(x) and (N′)_(y) is an oligonucleotide in which each consecutive N or N′ is joined to the next N or N′ by a covalent bond; wherein each of Z and Z′ is independently present or absent, but if present independently includes 1-5 consecutive nucleotides or non-nucleotide moieties or a combination thereof covalently attached at the 3′-terminus of the strand in which it is present; wherein z″ may be present or absent, but if present is a capping moiety covalently attached at the 5′-terminus of (N′)_(y); wherein each of x and y is independently an integer between 18 and 40; wherein the sequence of (N′)_(y) has complementary to the sequence of (N)_(x); and wherein (N)_(x) includes an antisense sequence to SEQ ID NO:1.

In some embodiments, (N), includes an antisense oligonucleotide present in Table 4. In other embodiments, (N)_(x) is selected from an antisense oligonucleotide present in Tables B or C.

In some embodiments, the covalent bond joining each consecutive N or N′ is a phosphodiester bond.

In some embodiments, x=y and each of x and y is 19, 20, 21, 22 or 23. In various embodiments, x=y=19.

In some embodiments of nucleic acid molecules (e.g., siNA molecules) as disclosed herein, the double-stranded nucleic acid molecule is a siRNA, siNA or a miRNA.

In some embodiments, the antisense and sense strands are selected from the sequence pairs set forth in

-   -   SERPINH1_(—)4 (SEQ ID NOS:195 and 220),     -   SERPINH1_(—)12 (SEQ ID NOS:196 and 221),     -   SERPINH1_(—)30 (SEQ ID NOS:199 and 224), and     -   SERPINH1_(—)58 (SEQ ID NOS:208 and 233).

In some embodiments, the antisense and sense strands are the sequence pairs set forth in SERPINH1_(—)4 (SEQ ID NOS:195 and 220). In some embodiments, the antisense and sense strands are the sequence pairs set forth in SERPINH1_(—)12 (SEQ ID NOS:196 and 221). In some embodiments, the antisense and sense strands are the sequence pairs set forth in SERPINH1_(—)30 (SEQ ID NOS:199 and 224). In some embodiments, the antisense and sense strands are the sequence pairs set forth in SERPINH1_(—)58 (SEQ ID NOS:208 and 233).

In some embodiments, the double-stranded nucleic acid molecules as a component of a pharmaceutical formulation comprise a DNA moiety or a mismatch to the target at position 1 of the antisense strand (5′-terminus). Such a structure is described herein. According to one embodiment provided are modified nucleic acid molecules having a structure (A2) set forth below:

(A2) 5′ N¹-(N)_(x) - Z     3′ (antisense strand) 3′ Z′-N²-(N′)_(y) -z″ 5′ (sense strand) wherein each of N², N and N′ is an unmodified or modified ribonucleotide, or an unconventional moiety; wherein each of (N)_(x) and (N′)_(y) is an oligonucleotide in which each consecutive N or N′ is joined to the adjacent N or N′ by a covalent bond; wherein each of x and y is independently an integer between 17 and 39; wherein the sequence of (N′)_(y) has complementarity to the sequence of (N)_(x) and (N)_(x) has complementarity to a consecutive sequence in a target RNA; wherein N¹ is covalently bound to (N)_(x) and is mismatched to the target RNA or is a complementary DNA moiety to the target RNA; wherein N¹ is a moiety selected from the group consisting of natural or modified uridine, deoxyribouridine, ribothymidine, deoxyribothymidine, adenosine or deoxyadenosine; wherein z″ may be present or absent, but if present is a capping moiety covalently attached at the 5′-terminus of N²—(N′)_(y); and wherein each of Z and Z′ is independently present or absent, but if p2resent is independently 1-5 consecutive nucleotides, consecutive non-nucleotide moieties or a combination thereof covalently attached at the 3′-terminus of the strand in which it is present.

In some embodiments, the sequence of (N′)_(y) is fully complementary to the sequence of (N)_(x). In various embodiments, sequence of N²—(N′)_(y) is complementary to the sequence of N¹—(N)_(x). In some embodiments, (N)_(x) comprises an antisense that is fully complementary to about 17 to about 39 consecutive nucleotides in a target RNA. In other embodiments, (N)_(x) comprises an antisense that is substantially complementary to about 17 to about 39 consecutive nucleotides in a target RNA.

In some embodiments, N¹ and N² form a Watson-Crick base pair. In some embodiments, N¹ and N² form a non-Watson-Crick base pair. In some embodiments, a base pair is formed between a ribonucleotide and a deoxyribonucleotide.

In some embodiments, x=y=18, x=y=19 or x=y=20. In preferred embodiments, x=y=18. When x=18 in N¹—(N)x, N¹ refers to position 1 and positions 2-19 are included in (N)₁₈. When y=18 in N²—(N′)_(y), N² refers to position 19 and positions 1-18 are included in (N′)₁₈.

In some embodiments, N¹ is covalently bound to (N)x and is mismatched to the target RNA. In various embodiments, N¹ is covalently bound to (N)_(x) and is a DNA moiety complementary to the target RNA.

In some embodiments, a uridine in position 1 of the antisense strand is substituted with an N¹ selected from adenosine, deoxyadenosine, deoxyuridine (dU), ribothymidine or deoxythymidine. In various embodiments, N¹ selected from adenosine, deoxyadenosine or deoxyuridine.

In some embodiments, guanosine in position 1 of the antisense strand is substituted with an N¹ selected from adenosine, deoxyadenosine, uridine, deoxyuridine, ribothymidine or deoxythymidine. In various embodiments, N¹ is selected from adenosine, deoxyadenosine, uridine or deoxyuridine.

In some embodiments, cytidine in position 1 of the antisense strand is substituted with an N¹ selected from adenosine, deoxyadenosine, uridine, deoxyuridine, ribothymidine or deoxythymidine. In various embodiments, N¹ is selected from adenosine, deoxyadenosine, uridine or deoxyuridine.

In some embodiments, adenosine in position 1 of the antisense strand is substituted with an N¹ selected from deoxyadenosine, deoxyuridine, ribothymidine or deoxythymidine. In various embodiments, N¹ selected from deoxyadenosine or deoxyuridine.

In some embodiments, N¹ and N² form a base pair between uridine or deoxyuridine, and adenosine or deoxyadenosine. In other embodiments, N¹ and N² form a base pair between deoxyuridine and adenosine.

In some embodiments, the double-stranded nucleic acid molecule as a component of a pharmaceutical formulation is a siRNA, siNA or a miRNA. The double-stranded nucleic acid molecules as provided herein are also referred to as “duplexes”.

In some embodiments (N)_(x) includes and antisense oligonucleotide present in Table 5. In some embodiments, x=y=18 and N¹—(N)_(x) includes an antisense oligonucleotide present in Table 4. In some embodiments x=y=19 or x=y=20. In certain preferred embodiments, x=y=18. In some embodiments x=y-18 and the sequences of N¹—(N)_(x) and N²—(N′)_(y) are selected from the pair of oligonucleotides set forth in Table 4. In some embodiments, x=y-18 and the sequences of N¹—(N)_(x) and N²—(N′)_(y) are selected from the pair of oligonucleotides set forth in Tables D and E. In some embodiments, the antisense and sense strands are selected from the sequence pairs set forth in

-   -   SERPINH1_(—)2 (SEQ ID NOS: 60 and 127),     -   SERPINH1_(—)6 (SEQ ID NOS: 63 and 130),     -   SERPINH1_(—)11 (SEQ ID NOS: 68 and 135),     -   SERPINH1_(—)13 (SEQ ID NOS: 69 and 136),     -   SERPINH1_(—)45 (SEQ ID NOS: 97 and 164),     -   SERPINH1_(—)45a (SEQ ID NOS: 98 and 165),     -   SERPINH1_(—)51 (SEQ ID NOS: 101 and 168),     -   SERPINH1_(—)51a (SEQ ID NOS: 105 and 172),     -   SERPINH1_(—)52 (SEQ ID NOS:102 and 169), and     -   SERPINH1_(—)86 (SEQ ID NOS: 123 and 190).

In some preferred embodiments, the antisense and sense strands are selected from the sequence pairs set forth in

-   -   SERPINH1_(—)2 (SEQ ID NOS: 60 and 127),     -   SERPINH1_(—)6 (SEQ ID NOS: 63 and 130),     -   SERPINH1_(—)45a (SEQ ID NOS: 98 and 165),     -   SERPINH1_(—)51 (SEQ ID NOS: 101 and 168), and     -   SERPINH1_(—)51a (SEQ ID NOS: 105 and 172).

In some preferred embodiments, the antisense and sense strands are selected from the sequence pairs set forth in SERPINH1_(—)2 (SEQ ID NOS: 60 and 127). In some embodiments, the antisense and sense strands are the sequence pairs set forth in SERPINH1_(—)6 (SEQ ID NOS: 63 and 130). In some embodiments, the antisense and sense strands are the sequence pairs set forth in SERPINH1_(—)11 (SEQ ID NOS: 68 and 135). In some embodiments, the antisense and sense strands are the sequence pairs set forth in SERPINH1_(—)13 (SEQ ID NOS: 69 and 136). In some embodiments, the antisense and sense strands are the sequence pairs set forth in SERPINH1_(—)45 (SEQ ID NOS: 97 and 164). In some embodiments, the antisense and sense strands are the sequence pairs set forth in SERPINH1_(—)45a (SEQ ID NOS: 98 and 165). In some embodiments, the antisense and sense strands are the sequence pairs set forth in SERPINH1_(—)51 (SEQ ID NOS: 101 and 168). In some embodiments, the antisense and sense strands are the sequence pairs set forth in SERPINH1_(—)51a (SEQ ID NOS: 105 and 172). In some embodiments, the antisense and sense strands are the sequence pairs set forth in SERPINH1_(—)52 (SEQ ID NOS:102 and 169). In some embodiments the antisense and sense strands are the sequence pairs set forth in (SEQ ID NOS: 123 and 190). In some preferred embodiments, the antisense and sense strands are selected from the sequence pairs set forth in SERPINH1_(—)2 (SEQ ID NOS: 60 and 127), SERPINH1_(—)6 (SEQ ID NOS: 63 and 130), SERPINH1_(—)45a (SEQ ID NOS: 98 and 165), SERPINH1_(—)51 (SEQ ID NOS: 101 and 168), and SERPINH1_(—)51a (SEQ ID NOS: 105 and 172).

In some embodiments, N¹ and N² form a Watson-Crick base pair. In other embodiments, N¹ and N² form a non-Watson-Crick base pair. In some embodiments N¹ is a modified riboadenosine or a modified ribouridine.

In some embodiments, N¹ and N² form a Watson-Crick base pair. In other embodiments, N¹ and N² form a non-Watson-Crick base pair. In certain embodiments, N¹ is selected from the group consisting of riboadenosine, modified riboadenosine, deoxyriboadenosine, modified deoxyriboadenosine. In other embodiments, N¹ is selected from the group consisting of ribouridine, deoxyribouridine, modified ribouridine, and modified deoxyribouridine.

In certain embodiments, position 1 in the antisense strand (5′-terminus) includes deoxyribouridine (dU) or adenosine. In some embodiments, N¹ is selected from the group consisting of riboadenosine, modified riboadenosine, deoxyriboadenosine, modified deoxyriboadenosine and N² is selected from the group consisting of ribouridine, deoxyribouridine, modified ribouridine, and modified deoxyribouridine. In certain embodiments, N¹ is selected from the group consisting of riboadenosine and modified riboadenosine and N² is selected from the group consisting of ribouridine and modified ribouridine.

In certain embodiments, N¹ is selected from the group consisting of ribouridine, deoxyribouridine, modified ribouridine, and modified deoxyribouridine and N² is selected from the group consisting of riboadenosine, modified riboadenosine, deoxyriboadenosine, and modified deoxyriboadenosine. In certain embodiments, N¹ is selected from the group consisting of ribouridine and deoxyribouridine and N² is selected from the group consisting of riboadenosine and modified riboadenosine. In certain embodiments, N¹ is ribouridine and N² is riboadenosine. In certain embodiments, N¹ is deoxyribouridine and N² is riboadenosine.

In some embodiments of Structure (A2), N¹ includes 2′OMe modified ribouracil or 2′OMe modified riboadenosine. In certain embodiments of structure (A2), N² includes a 2′OMe modified ribonucleotide or deoxyribonucleotide.

In some embodiments of Structure (A2), N¹ includes 2′OMe modified ribouracil or 2′OMe modified ribocytosine. In certain embodiments of structure (A2), N² includes a 2′OMe modified ribonucleotide.

In some embodiments each of N and N′ is an unmodified nucleotide. In some embodiments, at least one of N or N′ includes a chemically modified nucleotide or an unconventional moiety. In some embodiments, the unconventional moiety is selected from a mirror nucleotide, an abasic ribose moiety and an abasic deoxyribose moiety. In some embodiments, the unconventional moiety is a mirror nucleotide, preferably an L-DNA moiety. In some embodiments, at least one of N or N′ includes a 2′OMe modified ribonucleotide.

In some embodiments, the sequence of (N′)_(y) is fully complementary to the sequence of (N)_(x). In other embodiments, the sequence of (N′)_(y) is substantially complementary to the sequence of (N)_(x).

In some embodiments, (N), includes an antisense sequence that is fully complementary to about 17 to about 39 consecutive nucleotides in a target mRNA. In other embodiments, (N)_(x) includes an antisense that is substantially complementary to about 17 to about 39 consecutive nucleotides in a target mRNA.

In some embodiments of Structure A1 and Structure A2 the compound is blunt ended, for example wherein both Z and Z′ are absent. In an alternative embodiment, at least one of Z or Z′ is present. Z and Z′ independently include one or more covalently linked modified and or unmodified nucleotides, including deoxyribonucleotides and ribonucleotides, or an unconventional moiety for example inverted abasic deoxyribose moiety or abasic ribose moiety; a non-nucleotide C3, C4 or C5 moiety, an amino-6 moiety, a mirror nucleotide, and the like. In some embodiments each of Z and Z′ independently includes a C3 moiety or an amino-C6 moiety. In some embodiments Z′ is absent and Z is present and includes a non-nucleotide C3 moiety. In some embodiments Z is absent and Z′ is present and includes a non-nucleotide C3 moiety.

In some embodiments of Structure A1 and Structure A2, each N consists of an unmodified ribonucleotide. In some embodiments of Structure A1 and Structure A2, each N′ consists of an unmodified nucleotide. In preferred embodiments, at least one of N and N′ is a modified ribonucleotide or an unconventional moiety.

In other embodiments, the compound of Structure A1 or Structure A2 includes at least one ribonucleotide modified in the sugar residue. In some embodiments, the compound includes a modification at the 2′ position of the sugar residue. In some embodiments, the modification in the 2′ position includes the presence of an amino, a fluoro, an alkoxy or an alkyl moiety. In certain embodiments, the 2′ modification includes an alkoxy moiety. In preferred embodiments, the alkoxy moiety is a methoxy moiety (2′OMe). In some embodiments, the nucleic acid compound includes 2′OMe modified alternating ribonucleotides in one or both of the antisense and the sense strands. In other embodiments, the compound includes 2′OMe modified ribonucleotides in the antisense strand, (N)_(x) or N¹—(N)_(x), only. In certain embodiments, the middle ribonucleotide of the antisense strand; e.g. ribonucleotide in position 10 in a 19-mer strand is unmodified. In various embodiments, the nucleic acid compound includes at least 5 alternating 2′OMe modified and unmodified ribonucleotides. In additional embodiments, the compound of Structure A1 or Structure A2 includes modified ribonucleotides in alternating positions wherein each ribonucleotide at the 5′ and 3′-termini of (N)_(x) or N¹—(N)_(x) are modified in their sugar residues, and each ribonucleotide at the 5′ and 3′-termini of (N′)_(y) or N²—(N)_(y) are unmodified in their sugar residues.

In some embodiments, the double-stranded molecule as a component of a pharmaceutical formulation includes one or more of the following modifications

-   -   N in at least one of positions 5, 6, 7, 8, or 9 of the antisense         strand is selected from a 2′-5′-nucleotide or a mirror         nucleotide;     -   N′ in at least one of positions 9 or 10 of the sense strand is         selected from a 2′-5′-nucleotide and a pseudo-uridine; and     -   N′ in 4, 5, or 6 consecutive positions at the 3′-terminus         positions of (N′)_(y) comprises a 2′-5′-nucleotide.

In some embodiments, the double-stranded molecule includes a combination of the following modifications

-   -   the antisense strand includes a 2′-5′-nucleotide or a mirror         nucleotide in at least one of positions 5, 6, 7, 8, or 9; and     -   the sense strand includes at least one of a 2′-5′-nucleotide and         a pseudo-uridine in positions 9 or 10.

In some embodiments, the double-stranded molecule includes a combination of the following modifications

-   -   the antisense strand includes a 2′-5′-nucleotide or a mirror         nucleotide in at least one of positions 5, 6, 7, 8, or 9; and     -   the sense strand includes 4, 5, or 6 consecutive         2′-5′-nucleotides at the 3′-penultimate or 3′-terminal         positions.

In some embodiments, the sense strand ((N)_(x) or N¹—(N)_(x)) includes 1, 2, 3, 4, 5, 6, 7, 8, or 9 2′OMe modified ribonucleotides. In some embodiments, the antisense strand includes 2′OMe modified ribonucleotides at positions 2, 4, 6, 8, 11, 13, 15, 17 and 19. In other embodiments, antisense strand includes 2′OMe modified ribonucleotides at positions 1, 3, 5, 7, 9, 11, 13, 15, 17 and 19. In other embodiments, the antisense strand includes 2′OMe modified ribonucleotides at positions 3, 5, 7, 9, 11, 13, 15, 17 and 19. In some embodiments the antisense strand includes one or more 2′OMe modified pyrimidines. In some embodiments, all the pyrimidine nucleotides in the antisense strand are 2′OMe modified. In some embodiments, the sense strand includes 2′OMe modified pyrimidines.

In some embodiments of Structure A1 and Structure A2, neither the sense strand nor the antisense strand is phosphorylated at the 3′- and 5′-termini. In other embodiments, one or both of the sense strand or the antisense strand are phosphorylated at the 3′-termini.

In some embodiments of Structure A1 and Structure A2, (N)_(y) includes at least one unconventional moiety selected from a mirror nucleotide, a 2′-5′-nucleotide and a TNA. In some embodiments, the unconventional moiety is a mirror nucleotide. In various embodiments, the mirror nucleotide is selected from an L-ribonucleotide (L-RNA) and an L-deoxyribonucleotide (L-DNA). In preferred embodiments, the mirror nucleotide is L-DNA. In certain embodiments, the sense strand comprises an unconventional moiety in position 9 or 10 (from the 5′-terminus). In preferred embodiments, the sense strand includes an unconventional moiety in position 9 (from the 5′-terminus). In some embodiments, the sense strand is 19 nucleotides in length and comprises 4, 5, or 6 consecutive unconventional moieties in positions 15, (from the 5′-terminus). In some embodiments, the sense strand includes 4 consecutive 2′-5′-ribonucleotides in positions 15, 16, 17, and 18. In some embodiments, the sense strand includes 5 consecutive 2′-5′-ribonucleotides in positions 15, 16, 17, 18 and 19. In various embodiments, the sense strand further comprises Z′. In some embodiments, Z′ includes a C3OH moiety or a C3Pi moiety.

In some embodiments, of Structure A1 (N′)_(y) includes at least one L-DNA moiety. In some embodiments, x=y=19 and (N′)_(y), consists of unmodified ribonucleotides at positions 1-17 and 19 and one L-DNA at the 3′ penultimate position (position 18). In other embodiments, x=y=19 and (N′)_(y) consists of unmodified ribonucleotides at positions 1-16 and 19 and two consecutive L-DNA at the 3′ penultimate position (positions 17 and 18). In various embodiments, the unconventional moiety is a nucleotide joined to an adjacent nucleotide by a 2′-5′ internucleotide phosphate linkage. According to various embodiments, (N′)_(y) includes 2, 3, 4, 5, or 6 consecutive ribonucleotides at the 3′-terminus linked by 2′-5′ internucleotide linkages. In one embodiment, four consecutive nucleotides at the 3′-terminus of (N′)_(y) are joined by three 2′-5′ phosphodiester bonds, wherein one or more of the 2′-5′ nucleotides which form the 2′-5′ phosphodiester bonds further includes a 3′-O-methyl (3′OMe) sugar modification. Preferably the 3′-terminal nucleotide of (N′)_(y) includes a 2′OMe modification. In certain embodiments, x=y=19 and (N′)_(y) includes two or more consecutive nucleotides at positions 15, 16, 17, 18 and 19 include a nucleotide joined to an adjacent nucleotide by a 2′-5′ internucleotide bond (2′-5′ nucleotide). In various embodiments the nucleotide forming the 2′-5′ internucleotide bond includes a 3′ deoxyribose nucleotide or a 3′ methoxy nucleotide (3′ H or 3′OMe in place of a 3′ OH). In some embodiments x=y=19 and (N′)_(y) includes 2′-5′ nucleotides at positions 15, 16 and 17 such that adjacent nucleotides are linked by a 2′-5′ internucleotide bond between positions 15-16, 16-17 and 17-18; or at positions, 15, 16, 17, 18, and 19 such that adjacent nucleotides are linked by a 2′-5′ internucleotide bond between positions 15-16, 16-17, 17-18 and 18-19 and a 3′ OH is available at the 3′-terminal nucleotide or at positions 16, 17 and 18 such that adjacent nucleotides are linked by a 2′-5′ internucleotide bond between positions 16-17, 17-18 and 18-19. In some embodiments x=y=19 and (N′)_(y) includes 2′-5′-nucleotides at positions 16 and 17 or at positions 17 and 18 or at positions 15 and 17 such that adjacent nucleotides are linked by a 2′-5′ internucleotide bond between positions 16-17 and 17-18 or between positions 17-18 and 18-19 or between positions 15-16 and 17-18, respectively. In other embodiments, the pyrimidine ribonucleotides (rU, rC) in (N′)_(y) are substituted with nucleotides joined to the adjacent nucleotide by a 2′-5′ internucleotide bond. In some embodiments, the antisense and sense strands are selected from the sequence pairs set forth in SERPINH1_(—)4, SERPINH1_(—)12, SERPINH1_(—)18, SERPINH1_(—)30, SERPINH1_(—)58 or SERPINH1_(—)88, and x=y=19 and (N′)_(y) comprises five consecutive nucleotides at the 3′-terminus joined by four 2′-5′ linkages, specifically the linkages between the nucleotides position 15-16, 16-17, 17-18 and 18-19.

In some embodiments, the linkages include phosphodiester bonds. In some embodiments, the antisense and sense strands are selected from the sequence pairs set forth in SERPINH1_(—)4, SERPINH1_(—)12, SERPINH1_(—)18, SERPINH1_(—)30, SERPINH1_(—)58 or SERPINH1_(—)88 and x=y=19 and (N′)_(y) comprises five consecutive nucleotides at the 3′-terminus joined by four 2′-5′ linkages and optionally further includes Z′ and z′ independently selected from an inverted abasic moiety and a C3 alkyl [C3; 1,3-propanediol mono(dihydrogen phosphate)] cap. The C3 alkyl cap is covalently linked to the 3′ or 5′ terminal nucleotide. In some embodiments, the 3′ C3 terminal cap further comprises a 3′ phosphate. In some embodiments, the 3′ C3 terminal cap further comprises a 3′-terminal hydroxy group.

In some embodiments, the antisense and sense strands as components of a pharmaceutical formulation are selected from the sequence pairs set forth in SERPINH1_(—)4, SERPINH1_(—)12, SERPINH1_(—)18, SERPINH1_(—)30, SERPINH1_(—)58 or SERPINH1_(—)88 and x=y=19 and (N′)_(y) includes an L-DNA position 18; and (N′)_(y) optionally further includes Z′ and z′ independently selected from an inverted abasic moiety and a C3 alkyl (C3; 1,3-propanediol mono(dihydrogen phosphate)) cap.

In some embodiments, (N′)_(y) includes a 3′-terminal phosphate. In some embodiments, (N′)_(y) includes a 3′-terminal hydroxyl.

In some embodiments, the antisense and sense strands as components of a pharmaceutical formulation are selected from the sequence pairs set forth in SERPINH1_(—)4, SERPINH1_(—)12, SERPINH1_(—)18, SERPINH1_(—)30, SERPINH1_(—)58 or SERPINH1_(—)88 and x=y=19 and (N)_(x) includes 2′OMe modified ribonucleotides at positions 1, 3, 5, 7, 9, 11, 13, 15, 17, 19 or at positions 2, 4, 6, 8, 11, 13, 15, 17, 19. In some embodiments, the antisense and sense strands are selected from the sequence pairs set forth in SERPINH1_(—)4, SERPINH1_(—)12, SERPINH1_(—)18, SERPINH1_(—)30, SERPINH1_(—)58 and SERPINH1_(—)88 and x=y=19 and (N)_(x) includes 2′OMe modified pyrimidines. In some embodiments, all pyrimidines in (N)_(x) include the 2′OMe modification.

In some embodiments, the antisense and sense strands are selected from the sequence pairs set forth in SERPINH1_(—)2, SERPINH1_(—)6, SERPINH1_(—)11, SERPINH1_(—)13, SERPINH1_(—)45, SERPINH1_(—)45a, SERPINH1_(—)51, SERPIN51a, SERPINH1_(—)52 or SERPINH1_(—)86 and x=y=18 and N² is a riboadenosine moiety.

In some embodiments, the antisense and sense strands are selected from the sequence pairs set forth in SERPINH1_(—)2, SERPINH1_(—)6, SERPINH1_(—)11, SERPINH1_(—)13, SERPINH1_(—)45, SERPINH1_(—)45a, SERPINH1_(—)51, SERPIN51a, SERPINH1_(—)52 or SERPINH1_(—)86 and x=y=18, and N²—(N′)_(y) includes five consecutive nucleotides at the 3′-terminus joined by four 2′-5′ linkages, specifically the linkages between the nucleotides position 15-16, 16-17, 17-18 and 18-19. In some embodiments the linkages include phosphodiester bonds.

In some embodiments, the antisense and sense strands are selected from the sequence pairs set forth in SERPINH1_(—)2, SERPINH1_(—)6, SERPINH1_(—)11, SERPINH1_(—)13, SERPINH1_(—)45, SERPINH1_(—)45a, SERPINH1_(—)51, SERPINH1_(—)51a, SERPINH1_(—)52 or SERPINH1_(—)86 and x=y=18 and N²—(N′)_(y) includes five consecutive nucleotides at the 3′-terminus joined by four 2′-5′ linkages and optionally further includes Z′ and z′ independently selected from an inverted abasic moiety and a C3 alkyl [C3; 1,3-propanediol mono(dihydrogen phosphate)] cap.

In some embodiments, the antisense and sense strands are selected from the sequence pairs set forth in SERPINH1_(—)2, SERPINH1_(—)6, SERPINH1_(—)11, SERPINH1_(—)13, SERPINH1_(—)45, SERPINH1_(—)45a, SERPINH1_(—)51, SERPINH1_(—)51a, SERPINH1_(—)52 or SERPINH1_(—)86 and x=y=18 and N²—(N′)_(y) includes an L-DNA position 18; and (N′)_(y) optionally further includes Z′ and z′ independently selected from an inverted abasic moiety and a C3 alkyl [C3; 1,3-propanediol mono(dihydrogen phosphate)] cap.

In some embodiments, N²—(N′)_(y) comprises a 3′-terminal phosphate. In some embodiments, N²—(N′)_(y) comprises a 3′-terminal hydroxyl.

In some embodiments, the antisense and sense strands as components of a pharmaceutical formulation are selected from the sequence pairs set forth in SERPINH1_(—)2, SERPINH1_(—)6, SERPINH1_(—)11, SERPINH1_(—)13, SERPINH1_(—)45, SERPINH1_(—)45a, SERPINH1_(—)51, SERPINH1_(—)51a, SERPINH1_(—)52 or SERPINH1_(—)86 and x=y=18 and N¹—(N)_(x) includes 2′OMe modified ribonucleotides in positions 1, 3, 5, 7, 9, 11, 13, 15, 17, 19 or in positions 1, 3, 5, 9, 11, 13, 15, 17, 19, or in positions 3, 5, 9, 11, 13, 15, 17, or in positions 2, 4, 6, 8, 11, 13, 15, 17, 19. In some embodiments, the antisense and sense strands are selected from the sequence pairs set forth in SERPINH1_(—)2, SERPINH1_(—)6, SERPINH1_(—)11, SERPINH1_(—)13, SERPINH1_(—)45, SERPINH1_(—)45a, SERPINH1_(—)51, SERPINH1_(—)52 or SERPINH1_(—)86 and x=y=18 and N¹—(N)_(x) includes 2′OMe modified ribonucleotides at positions 11, 13, 15, 17 and 19. In some embodiments, the antisense and sense strands are selected from the sequence pairs set forth in SERPINH1_(—)2, SERPINH1_(—)6, SERPINH1_(—)11, SERPINH1_(—)13, SERPINH1_(—)45, SERPINH1_(—)45a, SERPINH1_(—)51, SERPINH1_(—)51a, SERPINH1_(—)52 or SERPINH1_(—)86 and x=y=18 and N¹—(N)_(x) includes 2′OMe modified ribonucleotides in positions 1, 3, 5, 7, 9, 11, 13, 15, 17, 19 or in positions 3, 5, 7, 9, 11, 13, 15, 17, 19. In some embodiments, the antisense and sense strands are selected from the sequence pairs set forth in SERPINH1_(—)2, SERPINH1_(—)6, SERPINH1_(—)11, SERPINH1_(—)13, SERPINH1_(—)45, SERPINH1_(—)45a, SERPINH1_(—)51, SERPINH1_(—)52 or SERPINH1_(—)86 and x=y=18 and N¹—(N)_(x) includes 2′OMe modified ribonucleotides in positions 2, 4, 6, 8, 11, 13, 15, 17, 19.

In some embodiments, the antisense and sense strands as components of a pharmaceutical formulation are selected from the sequence pairs set forth in SERPINH1_(—)2, SERPINH1_(—)6, SERPINH1_(—)11, SERPINH1_(—)13, SERPINH1_(—)45, SERPINH1_(—)45a, SERPINH1_(—)51, SERPINH1_(—)51a, SERPINH1_(—)52 or SERPINH1_(—)86 and x=y=18 and N¹—(N)^(x) includes 2′OMe modified pyrimidines. In some embodiments, all pyrimidines in (N)_(x) include the 2′OMe modification. In some embodiments, the antisense strand further includes an L-DNA or a 2′-5′ nucleotide in position 5, 6 or 7. In other embodiments, the antisense strand further includes a ribonucleotide which generates a 2′-5′ internucleotide linkage in between the ribonucleotides in positions 5-6 or 6-7.

In additional embodiments, N¹—(N)_(x) further includes Z wherein Z includes a non-nucleotide overhang. In some embodiments the non-nucleotide overhang is C3-C3 [1,3-propanediol mono(dihydrogen phosphate)]2.

In some embodiments, of Structure A2, (N)_(y) includes at least one L-DNA moiety. In some embodiments x=y=18 and (N′)_(y) consists of unmodified ribonucleotides at positions 1-16 and 18 and one L-DNA at the 3′ penultimate position (position 17). In other embodiments, x=y=18 and (N′)_(y) consists of unmodified ribonucleotides at position 1-15 and 18 and two consecutive L-DNA at the 3′ penultimate position (positions 16 and 17). In various embodiments, the unconventional moiety is a nucleotide joined to an adjacent nucleotide by a 2′-5′ internucleotide phosphate linkage. According to various embodiments, (N′)_(y) includes 2, 3, 4, 5, or 6 consecutive ribonucleotides at the 3′-terminus linked by 2′-5′ internucleotide linkages. In one embodiment, four consecutive nucleotides at the 3′-terminus of (N′)_(y) are joined by three 2′-5′ phosphodiester bonds, wherein one or more of the 2′-5′ nucleotides which form the 2′-5′ phosphodiester bonds further includes a 3′-O-methyl (3′OMe) sugar modification. Preferably, the 3′-terminal nucleotide of (N′)_(y) includes a 2′OMe modification. In certain embodiments, x=y=18 and in (N′)y two or more consecutive nucleotides at positions 14, 15, 16, 17, and 18 include a nucleotide joined to an adjacent nucleotide by a 2′-5′ internucleotide bond. In various embodiments, the nucleotide forming the 2′-5′ internucleotide bond includes a 3′ deoxyribose nucleotide or a 3′ methoxy nucleotide. In some embodiments, x=y=18 and (N′)_(y) includes nucleotides joined to the adjacent nucleotide by a 2′-5′ internucleotide bond between positions 15-16, 16-17 and 17-18 or between positions 16-17 and 17-18. In some embodiments, x=y=18 and (N′)_(y) includes nucleotides joined to the adjacent nucleotide by a 2′-5′ internucleotide bond between positions 14-15, 15-16, 16-17, and 17-18 or between positions 15-16, 16-17, and 17-18 or between positions 16-17 and 17-18 or between positions 17-18 or between positions 15-16 and 17-18. In other embodiments, the pyrimidine ribonucleotides (rU, rC) in (N′)_(y) are substituted with nucleotides joined to the adjacent nucleotide by a 2′-5′ internucleotide bond.

In some embodiments, the antisense and sense strands are selected from the oligonucleotide pairs set forth in Table 5 and identified herein as SERPINH1_(—)2 (SEQ ID NOS: 60 and 127), SERPINH1_(—)6 (SEQ ID NOS: 63 and 130), SERPINH1_(—)45a (SEQ ID NOS: 98 and 165), SERPINH1_(—)51 (SEQ ID NOS: 101 and 168), and SERPINH1_(—)51a (SEQ ID NOS: 105 and 172).

In some embodiments, the double-stranded nucleic acid molecule as a component of a pharmaceutical formulation includes the antisense strand set forth in SEQ ID NO:127 and sense strand set forth in SEQ ID NO:60; identified herein as SERPINH1_(—)2. In some embodiments the double-stranded nucleic acid molecule has the structure

wherein each “|” represents base pairing between the ribonucleotides; wherein each of A, C, G, U is independently an unmodified or modified ribonucleotide, or an unconventional moiety; wherein each of Z and Z′ is independently present or absent, but if present is independently 1-5 consecutive nucleotides or non-nucleotide moieties or a combination thereof covalently attached at the 3′-terminus of the strand in which it is present; and wherein z″ may be present or absent, but if present is a capping moiety covalently attached at the 5′-terminus of N²—(N′)_(y).

In some embodiments, provided is a double-stranded nucleic acid molecule wherein the antisense strand (SEQ ID NO:127) includes one or more 2′OMe modified pyrimidines and or purines, a 2′-5′-ribonucleotide in position 5, 6, 7 or 8, and a 3′-terminal nucleotide or non-nucleotide overhang. In some embodiments, the sense strand (SEQ ID NO:60) includes 4 or 5 consecutive 2′-5′-nucleotides at the 3′-terminal or penultimate positions, a nucleotide or non-nucleotide moiety covalently attached at the 3′-terminus and a cap moiety covalently attached at the 5′-terminus. In other embodiments, the sense strand (SEQ ID NO:60) includes one or more 2′OMe pyrimidine, a nucleotide or non-nucleotide moiety covalently attached at the 3′-terminus and a cap moiety covalently attached at the 5′-terminus

In some embodiments, provided is a double-stranded nucleic acid molecule wherein the antisense strand (SEQ ID NO:127) includes 2′OMe modified ribonucleotides at positions 1, 3, 5, 9, 11, 15, 17 and 19; a 2′-5′-ribonucleotide at position 7; and a C3Pi-C3OH moiety covalently attached to the 3′-terminus; and the sense strand (SEQ ID NO:60) is selected from a sense strand which includes

-   -   2′-5′-ribonucleotides at positions 15, 16, 17, 18 and 19; a C3OH         3′-terminal non-nucleotide overhang; and an inverted abasic         deoxyribonucleotide moiety covalently attached at the         5′-terminus; or     -   2′-5′-ribonucleotides at positions 15, 16, 17, 18 and 19; a         3′-terminal phosphate; and an inverted abasic         deoxyribonucleotide moiety covalently attached at the         5′-terminus; or     -   2′OMe modified ribonucleotides at positions 5, 7, 13, and 16; a         2′-5′-ribonucleotide at position 18; a C3-OH moiety covalently         attached at the 3′-terminus; and an inverted abasic         deoxyribonucleotide moiety covalently attached at the         5′-terminus; or     -   2′OMe modified ribonucleotides at positions 7, 13, 16 and 18; a         2′-5′-ribonucleotide at position 9; a C3OH moiety covalently         attached at the 3′-terminus; and an inverted abasic         deoxyribonucleotide moiety covalently attached at the         5′-terminus; or     -   2′-5′-ribonucleotides at positions 15, 16, 17, 18, and 19; a         C3-Pi moiety covalently attached at the 3′-terminus; and an         inverted abasic deoxyribonucleotide moiety covalently attached         at the 5′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the antisense strand (SEQ ID NO:127) includes 2′OMe modified ribonucleotides at positions 1, 3, 5, 9, 11, 15, 17, 19; a 2′-5′-ribonucleotide at position 7; and a C3Pi-C3OH moiety covalently attached to the 3′-terminus; and the sense strand (SEQ ID NO:60) includes 2′-5′-ribonucleotides at positions 15, 16, 17, 18, and 19; a C3 3′-terminal overhang; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the antisense strand (SEQ ID NO:127) includes 2′OMe modified ribonucleotides at positions 1, 3, 5, 9, 11, 15, 17, 19; a 2′-5′-ribonucleotide at position 7; and a C3Pi-C3OH 3′-terminal overhang; and the sense strand (SEQ ID NO:60) includes 2′-5′-ribonucleotides at positions 15, 16, 17, 18, and 19; a 3′-terminal phosphate; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the antisense strand (SEQ ID NO:127) includes 2′OMe modified ribonucleotides at positions 1, 3, 5, 9, 11, 15, 17, 19; a 2′-5′-ribonucleotide at position 7 and a C3Pi-C3OH moiety covalently attached to the 3′-terminus; and the sense strand (SEQ ID NO:60) includes 2′OMe modified ribonucleotides at positions 5, 7, 13, and 16; a 2′-5′-ribonucleotide at position 18; a C3-OH moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the antisense strand (SEQ ID NO:127) includes 2′OMe modified ribonucleotides at positions 1, 3, 5, 9, 11, 15, 17, 19; a 2′-5′-ribonucleotide at position 7 and a C3Pi-C3OH moiety covalently attached to the 3′-terminus; and the sense strand (SEQ ID NO:60) includes 2′OMe modified ribonucleotides at positions 7, 13, 16 and 18; a 2′-5′-ribonucleotide at position 9; a C3-OH moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the antisense strand (SEQ ID NO:127) includes 2′OMe modified ribonucleotides at positions 1, 3, 5, 9, 11, 15, 17, 19; a 2′-5′-ribonucleotide at position 7; and a C3Pi-C3OH moiety covalently attached to the 3′-terminus; and the sense strand (SEQ ID NO:60) includes 2′-5′-ribonucleotides at positions 15, 16, 17, 18, and 19; a C3-Pi moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus.

In some embodiments provided herein is a double-stranded nucleic acid molecule wherein the antisense strand (SEQ ID NO:127) includes 2′OMe modified ribonucleotides at positions 1, 3, 5, 9, 11, 13, 15, 17, 19; and a C3-C3 3′-terminal overhang; and the sense strand (SEQ ID NO:60) includes 2′OMe modified ribonucleotides at positions 7, 9, 13, 16 and 18; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus

In some embodiments provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:60) includes 2′-5′-ribonucleotides at positions 15, 16, 17, 18, and 19; a 3′-terminal phosphate; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:127) includes an antisense strand selected from one of

-   -   2′OMe modified ribonucleotides at positions 1, 3, 5, 7, 9, 11,         13, 15, 17, 19; and a C3Pi-C3OH moiety covalently attached to         the 3′-terminus; or     -   2′OMe modified ribonucleotides at positions 1, 3, 6, 8, 10, 12,         14, 17, 18; and a C3Pi-C3OH moiety covalently attached to the         3′-terminus.

In some embodiments provided herein is a double-stranded nucleic acid molecule as a component of a pharmaceutical formulation which includes the antisense strand set forth in SEQ ID NO:130 and the sense strand set forth in SEQ ID NO:63; identified herein as SERPINH1_(—)6. In some embodiments the duplex comprises the structure

wherein each “|” represents base pairing between the ribonucleotides; wherein each of A, C, G, U is independently an unmodified or modified ribonucleotide, or an unconventional moiety; wherein each of Z and Z′ is independently present or absent, but if present is independently 1-5 consecutive nucleotides or non-nucleotide moieties or a combination thereof covalently attached at the 3′-terminus of the strand in which it is present; and wherein z″ may be present or absent, but if present is a capping moiety covalently attached at the 5′-terminus of N²—(N′)_(y).

In some embodiments provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:63) includes one or more 2′OMe modified pyrimidines; a 3′-terminal nucleotide or non-nucleotide overhang; and cap moiety covalently attached at the 5′-terminus. In some embodiments, the antisense strand (SEQ ID NO:130) includes one or more 2′OMe modified pyrimidine; a nucleotide or non-nucleotide moiety covalently attached at the 3′-terminus; and a cap moiety covalently attached at the 5′-terminus

In some embodiments provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:63) includes 2′OMe modified ribonucleotides at positions 2, 14 and 18; a C3OH or C3Pi moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:130) is selected from an antisense strand that includes

-   -   2′OMe modified ribonucleotides in positions 1, 3, 5, 9, 11, 13,         15 and 17; a 2′-5′-ribonucleotide at position 7; and a C3Pi-C3OH         moiety covalently attached to the 3′-terminus; or     -   2′OMe modified ribonucleotides in positions 1, 3, 5, 7, 9, 12,         13 and 17; a 2′-5′-ribonucleotide at position 7; and a C3Pi-C3OH         moiety covalently attached to the 3′-terminus; or     -   2′OMe modified ribonucleotides in positions 3, 5, 9, 11, 13, 15         and 17; a 2′-5′-ribonucleotide at position 7; and a C3Pi-C3OH         moiety covalently attached to the 3′-terminus; or     -   2′OMe modified ribonucleotides in positions 3, 5, 9, 11, 13, 15         and 17; a dU in position 1; a 2′-5′-ribonucleotide in position         7; and a C3Pi-C3OH moiety covalently attached to the 3′-terminus

In some embodiments provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:63) includes 2′OMe modified ribonucleotides in positions 2, 14 and 18; a C3-OH moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:130) includes 2′OMe modified ribonucleotides in positions 1, 3, 5, 9, 11, 13, 15 and 17; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH moiety covalently attached to the 3′-terminus

In some embodiments provided herein is a duplex oligonucleotide molecule wherein the sense strand (SEQ ID NO:63) includes 2′OMe modified ribonucleotides in positions 14 and 18 and optionally in position 2; a C3-OH moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:130) includes 2′OMe modified ribonucleotides in positions 1, 3, 5, 7, 9, 12, 13, and 17; a 2′-5′-ribonucleotide at position 7; and a C3Pi-C3OH moiety covalently attached to the 3′-terminus

In some embodiments provided herein is a duplex oligonucleotide molecule wherein the sense strand (SEQ ID NO:63) includes 2′OMe modified ribonucleotides in positions 14 and 18; a C3-OH moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:130) is selected from an antisense strand which includes

-   -   2′OMe modified ribonucleotides in positions 1, 3, 5, 9, 11, 13,         15 and 17; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3Pi         or C3Pi-C3OH moiety covalently attached to the 3′-terminus; or     -   2′OMe modified ribonucleotides in positions 1, 3, 5, 7, 9, 12,         13, and 17; a 2′-5′-ribonucleotide in position 7; and a         C3Pi-C3Pi or C3Pi-C3OH moiety covalently attached to the         3′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:63) includes 2′OMe modified ribonucleotides in positions 14 and 18; a C3-OH moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:130) includes 2′OMe modified ribonucleotides in positions 1, 3, 5, 9, 11, 13, 15 and 17; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH moiety covalently attached to the 3′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:63) includes 2′OMe modified ribonucleotides in positions 14 and 18; a C3-OH moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:130) includes 2′OMe modified ribonucleotides in positions 1, 3, 5, 7, 9, 12, 13, and 17; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH 3′-terminal overhang.

In some embodiments, the duplex s a component of a pharmaceutical formulation includes the antisense strand set forth in SEQ ID NO:165 and sense strand set forth in SEQ ID NO:98; identified herein as SERPINH1_(—)45a. In some embodiments, the duplex comprises the structure

wherein each “|” represents base pairing between the ribonucleotides; wherein each of A, C, G, U is independently an unmodified or modified ribonucleotide, or an unconventional moiety; wherein each of Z and Z′ is independently present or absent, but if present is independently 1-5 consecutive nucleotides or non-nucleotide moieties or a combination thereof covalently attached at the 3′-terminus of the strand in which it is present; and wherein z″ may be present or absent, but if present is a capping moiety covalently attached at the 5′-terminus of N²—(N′)_(y)

In some embodiments, the sense strand (SEQ ID NO:98) includes 2′-5′-ribonucleotides in positions 15, 16, 17, and 18 or 15, 16, 17, 18, and 19; a nucleotide or non-nucleotide moiety covalently attached at the 3′-terminus; and a cap moiety covalently attached at the 5′-terminus. In some embodiments the antisense strand (SEQ ID NO:165) includes 2′OMe modified pyrimidine and or purines; a 2′-5′ nucleotide in position 5, 6, 7, or 8; and a nucleotide or non-nucleotide moiety covalently attached at the 3′-terminus

In some embodiments, the sense strand (SEQ ID NO:98) includes 2′-5′-ribonucleotides in positions 15, 16, 17, 18, and 19; a C3Pi or C3-OH 3′-terminal non-nucleotide moiety; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:165) includes an antisense strand selected from one of

-   -   2′OMe modified ribonucleotides in positions 2, 4, 6, 8, 11, 13,         15, 17, and 19; a 2′-5′-ribonucleotide in position 7; and a         C3Pi-C3Pi or C3Pi-C3OH 3′-terminal overhang; or     -   2′OMe modified ribonucleotides in positions 2, 4, 6, 8, 11, 13,         15, 17 and 19; and a C3Pi-C3Pi or C3Pi-C3OH 3′-terminal         overhang;     -   2′OMe modified ribonucleotides in positions 1, 3, 5, 9, 11, 13,         15, 17, and 19; a 2′-5′-ribonucleotide in position 7; and a         C3Pi-C3Pi or C3Pi-C3OH 3′-terminal overhang; or     -   2′OMe modified ribonucleotides in positions 1, 3, 5, 7, 9, 11,         13, 15, 17 and 19; and a C3Pi-C3Pi or C3Pi-C3OH 3′-terminal         overhang.

Provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:98) includes 2′-5′-ribonucleotides in positions 15, 16, 17, 18, and 19; a C3-OH 3′-terminal moiety; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:165) includes 2′OMe modified ribonucleotides in positions 2, 4, 6, 8, 11, 13, 15, 17, and 19; a 2′-5′-ribonucleotide in position 7; and a C3Pi-COH 3′-terminal overhang.

In some embodiments, the double-stranded nucleic acid molecule s a component of a pharmaceutical formulation includes the antisense strand set forth in SEQ ID NO:168 and sense strand set forth in SEQ ID NO:101; identified herein as SERPINH1_(—)51. In some embodiments, the duplex comprises the structure

wherein each “|” represents base pairing between the ribonucleotides; wherein each of A, C, G, U is independently an unmodified or modified ribonucleotide, or an unconventional moiety; wherein each of Z and Z′ is independently present or absent, but if present is independently 1-5 consecutive nucleotides or non-nucleotide moieties or a combination thereof covalently attached at the 3′-terminus of the strand in which it is present; and wherein z″ may be present or absent, but if present is a capping moiety covalently attached at the 5′-terminus of N²—(N′)_(y).

In some embodiments provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:101) includes 2′OMe modified pyrimidines, optionally a 2′-5′-ribonucleotide in position 9 or 10; a nucleotide or non-nucleotide moiety covalently attached at the 3′-terminus; and optionally a cap moiety covalently attached at the 5′-terminus. In some embodiments the antisense strand (SEQ ID NO:168) includes 2′OMe modified pyrimidine and or purines; a 2′-5′ nucleotide in position 5, 6, 7, or 8; and a nucleotide or non-nucleotide moiety covalently attached at the 3′-terminus.

In some embodiments provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:101) includes 2′OMe modified pyrimidines in positions 4, 11, 13, and 17; optionally a 2′-5′-ribonucleotide in position 9 or 10; a C3Pi or C3OH non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:168) is selected from an antisense strand which includes

-   -   a) 2′OMe modified ribonucleotides in positions 1, 8, and 15; a         2′-5′-ribonucleotide in position 6 or 7; and a C3Pi-C3OH         overhang covalently attached at the 3′-terminus; or     -   b) 2′OMe modified ribonucleotides in positions 1, 4, 8, 13 and         15; a 2′-5′-ribonucleotide in position 6 or 7; and a C3Pi-C3OH         overhang covalently attached at the 3′-terminus; or     -   c) 2′OMe modified ribonucleotides in positions 1, 4, 8, 11 and         15; a 2′-5′-ribonucleotide in position 6; and a C3Pi-C3OH         overhang covalently attached at the 3′-terminus; or     -   d) 2′OMe modified ribonucleotides in positions 1, 3, 8, 12, 13,         and 15; a 2′-5′-ribonucleotide in position 6; and a C3Pi-C3OH         moiety covalently attached at the 3′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:101) includes 2′OMe modified ribonucleotides in positions 4, 11, 13, and 17; optionally a 2′-5′-ribonucleotide in position 9; a C3-OH non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:168) includes 2′OMe modified ribonucleotides in positions 1, 8, and 15; a 2′-5′-ribonucleotide in position 6; and a C3Pi-C3OH moiety covalently attached at the 3′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:101) includes 2′OMe modified ribonucleotides in positions 4, 11, 13, and 17; optionally a 2′-5′-ribonucleotide in position 9; a C3-OH non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:168) includes 2′OMe modified ribonucleotides in positions 1, 4, 8, 13 and 15; a 2′-5′-ribonucleotide in position 6; and a C3Pi-C3OH moiety covalently attached at the 3′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:101) includes 2′OMe modified ribonucleotides in positions 4, 11, 13, and 17; a 2′-5′-ribonucleotide in position 9; a C3OH non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:168) includes 2′OMe modified ribonucleotides in positions 1, 4, 8, 11 and 15; a 2′-5′-ribonucleotide in position 6; and a C3Pi-C3OH moiety covalently attached at the 3′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:101) includes 2′OMe modified ribonucleotides in positions 4, 11, 13, and 17; a 2′-5′-ribonucleotide in position 9; a C3OH non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:168) includes 2′OMe modified ribonucleotides in positions 1, 3, 8, 12, 13, and 15; a 2′-5′-ribonucleotide in position 6; and a C3Pi-C3OH moiety covalently attached at the 3′-terminus

In some embodiments, the double-stranded nucleic acid molecule is a component of a pharmaceutical formulation includes the antisense strand set forth in SEQ ID NO:168 and sense strand set forth in SEQ ID NO:101; identified herein as SERPINH1_(—)51a. In some embodiments the duplex comprises the structure

wherein each “|” represents base pairing between the ribonucleotides; wherein each of A, C, G, U is independently an unmodified or modified ribonucleotide, or an unconventional moiety; wherein each of Z and Z′ is independently present or absent, but if present is independently 1-5 consecutive nucleotides or non-nucleotide moieties or a combination thereof covalently attached at the 3′-terminus of the strand in which it is present; and wherein z″ may be present or absent, but if present is a capping moiety covalently attached at the 5′-terminus of N²—(N′)_(y).

In some embodiments provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:105) includes 2′OMe modified pyrimidines; optionally a 2′-5′-ribonucleotide in position 9 or 10; a nucleotide or non-nucleotide moiety covalently attached at the 3′-terminus; and optionally a cap moiety covalently attached at the 5′-terminus. In some embodiments the antisense strand (SEQ ID NO:172) includes 2′OMe modified pyrimidine and or purines; a 2′-5′ nucleotide in position 5, 6, 7, or 8; and a nucleotide or non-nucleotide moiety covalently attached at the 3′-terminus

In some embodiments provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:105) includes 2′OMe modified pyrimidines in positions 4, 11, 13, and 17; optionally a 2′-5′-ribonucleotide in position 9 or 10; a C3Pi or C3OH non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:172) is selected from an antisense strand which includes

-   -   a) 2′OMe modified ribonucleotides in positions 8, and 15; a         2′-5′-ribonucleotide in position 6 or 7; and a C3Pi-C3OH moiety         covalently attached at the 3′-terminus; or     -   b) 2′OMe modified ribonucleotides in positions 4, 8, 13 and 15;         a 2′-5′-ribonucleotide in position 6 or 7; and a C3Pi-C3OH         moiety covalently attached at the 3′-terminus; or     -   c) 2′OMe modified ribonucleotides in positions 4, 8, 11 and 15;         a 2′-5′-ribonucleotide in position 6; and a C3Pi-C3OH moiety         covalently attached at the 3′-terminus; or     -   d) 2′OMe modified ribonucleotides in positions 3, 8, 12, 13, and         15; a 2′-5′-ribonucleotide in position 6; and a C3Pi-C3OH moiety         covalently attached at the 3′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:105) includes 2′OMe modified ribonucleotides in positions 4, 11, 13, and 17; optionally a 2′-5′-ribonucleotide in position 9; a C3-OH non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:172) includes 2′OMe modified ribonucleotides in positions 8 and 15; a 2′-5′-ribonucleotide in position 6; and a C3Pi-C3OH moiety covalently attached at the 3′-terminus.

Provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:105) includes 2′OMe modified ribonucleotides in positions 4, 11, 13, and 17; optionally a 2′-5′-ribonucleotide in position 9; a C3-OH non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:172) includes 2′OMe modified ribonucleotides in positions 4, 8, 13 and 15; a 2′-5′-ribonucleotide in position 6; and a C3Pi-C3OH moiety covalently attached at the 3′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:105) includes 2′OMe modified ribonucleotides in positions 4, 11, 13, and 17; a 2′-5′-ribonucleotide in position 9; a C3-OH non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:172) includes 2′OMe modified ribonucleotides in positions 4, 8, 11 and 15; a 2′-5′-ribonucleotide in position 6; and a C3Pi-C3OH moiety covalently attached at the 3′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:105) includes 2′OMe modified ribonucleotides in positions 4, 11, 13, and 17; a 2′-5′-ribonucleotide in position 9; a C3OH non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:172) includes 2′OMe modified ribonucleotides in positions 3, 8, 12, 13, and 15; a 2′-5′-ribonucleotide in position 6; and a C3Pi-C3OH moiety covalently attached at the 3′-terminus

In some embodiments the antisense and sense strands are selected from the oligonucleotide pairs set forth in Table 5 and identified herein as SERPINH1_(—)4 (SEQ ID NOS: 195 and 220) and SERPINH1_(—)12 (SEQ ID NOS: 196 and 221).

In some embodiments the double-stranded nucleic acid molecule is a component of a pharmaceutical formulation includes the antisense strand set forth in SEQ ID NO:220 and sense strand set forth in SEQ ID NO:195; identified herein as SERPINH1_(—)4. In some embodiments the double-stranded nucleic acid molecule has the structure

wherein each “|” represents base pairing between the ribonucleotides; wherein each of A, C, G, U is independently an unmodified or modified ribonucleotide, or an unconventional moiety; wherein each of Z and Z′ is independently present or absent, but if present is independently 1-5 consecutive nucleotides or non-nucleotide moieties or a combination thereof covalently attached at the 3′-terminus of the strand in which it is present; and wherein z″ may be present or absent, but if present is a capping moiety covalently attached at the 5′-terminus of N²—(N′)_(y).

In some embodiments provided is a double-stranded nucleic acid molecule wherein the antisense strand (SEQ ID NO:220) includes 2′OMe modified ribonucleotides in positions 3, 5, 9, 11, 15, 17 and 19, a 2′-5′-ribonucleotide in position 7, and a C3Pi-C3OH moiety covalently attached to the 3′-terminus; and the sense strand (SEQ ID NO:195) is selected from a sense strand which includes

-   -   a) 2′-5′-ribonucleotides in positions 15, 16, 17, 18 and 19, a         C3OH moiety covalently attached to the 3′-terminus; and an         inverted abasic deoxyribonucleotide moiety covalently attached         at the 5′-terminus; or     -   b) 2′-5′-ribonucleotides in positions 15, 16, 17, 18 and 19, a         3′-terminal phosphate; and an inverted abasic         deoxyribonucleotide moiety covalently attached at the         5′-terminus; or     -   c) 2′OMe modified ribonucleotides in positions 5, 7, 13, and 16;         a 2′-5′-ribonucleotide in position 18; a C3OH moiety covalently         attached at the 3′-terminus; and an inverted abasic         deoxyribonucleotide moiety covalently attached at the         5′-terminus; or     -   d) 2′OMe modified ribonucleotides in positions 7, 13, 16 and 18;         a 2′-5′-ribonucleotide in position 9; a C3OH moiety covalently         attached at the 3′-terminus; and an inverted abasic         deoxyribonucleotide moiety covalently attached at the         5′-terminus; or     -   e) 2′-5′-ribonucleotides in positions 15, 16, 17, 18, and 19; a         C3Pi moiety covalently attached at the 3′-terminus; and an         inverted abasic deoxyribonucleotide moiety covalently attached         at the 5′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the antisense strand (SEQ ID NO:220) includes 2′OMe modified ribonucleotides in positions 3, 5, 9, 11, 15, 17, 19; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH moiety covalently attached to the 3′-terminus; and the sense strand (SEQ ID NO:195) includes 2′-5′-ribonucleotides in positions 15, 16, 17, 18, and 19; a C3 moiety covalently attached to the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the antisense strand (SEQ ID NO:220) includes 2′OMe modified ribonucleotides in positions 3, 5, 9, 11, 15, 17, 19; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH moiety covalently attached to the 3′-terminus; and the sense strand (SEQ ID NO:195) includes 2′-5′-ribonucleotides in positions 15, 16, 17, 18, and 19; a 3′-terminal phosphate; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the antisense strand (SEQ ID NO:220) includes 2′OMe modified ribonucleotides in positions 3, 5, 9, 11, 15, 17, 19; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH moiety covalently attached to the 3′-terminus; and the sense strand (SEQ ID NO:195) includes 2′OMe modified ribonucleotides in positions 5, 7, 13, and 16; a 2′-5′-ribonucleotide in position 18; a C3OH moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the antisense strand (SEQ ID NO:220) includes 2′OMe modified ribonucleotides in positions 3, 5, 9, 11, 15, 17, 19; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH moiety covalently attached to the 3′-terminus; and the sense strand (SEQ ID NO:195) includes 2′OMe modified ribonucleotides in positions 7, 13, 16 and 18; a 2′-5′-ribonucleotide in position 9; a C3OH moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the antisense strand (SEQ ID NO:220) includes 2′OMe modified ribonucleotides in positions 3, 5, 9, 11, 15, 17, 19; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH moiety covalently attached to the 3′-terminus; and the sense strand (SEQ ID NO:195) includes 2′-5′-ribonucleotides in positions 15, 16, 17, 18, and 19; a C3Pi moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus

In some embodiments provided herein is a double-stranded nucleic acid molecule wherein the antisense strand (SEQ ID NO:220) includes 2′OMe modified ribonucleotides in positions 1, 3, 5, 9, 11, 13, 15, 17, 19; and a C3Pi-C3OH moiety covalently attached to the 3′-terminus; and the sense strand (SEQ ID NO:195) includes 2′OMe modified ribonucleotides in positions 7, 9, 13, 16 and 18; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus

In some embodiments provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:195) includes 2′-5′-ribonucleotides in positions 15, 16, 17, 18, and 19; a 3′-terminal phosphate and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:220) includes an antisense strand selected from one of

-   -   a) 2′OMe modified ribonucleotides in positions 3, 5, 7, 9, 11,         13, 15, 17, 19; and a C3Pi-C3OH moiety covalently attached to         the 3′-terminus; or     -   b) 2′OMe modified ribonucleotides in positions 1, 3, 6, 8, 10,         12, 14, 17, 18; and a C3Pi-C3OH moiety covalently attached to         the 3′-terminus.

In some embodiments provided herein is a double-stranded nucleic acid molecule s a component of a pharmaceutical formulation which includes the antisense strand set forth in SEQ ID NO:130 and the sense strand set forth in SEQ ID NO:63; identified herein as SERPINH1_(—)12. In some embodiments the duplex comprises the structure

wherein each “|” represents base pairing between the ribonucleotides; wherein each of A, C, G, U is independently an unmodified or modified ribonucleotide, or an unconventional moiety; wherein each of Z and Z′ is independently present or absent, but if present is independently 1-5 consecutive nucleotides or non-nucleotide moieties or a combination thereof covalently attached at the 3′-terminus of the strand in which it is present; and wherein z″ may be present or absent, but if present is a capping moiety covalently attached at the 5′-terminus of N²—(N′)_(y).

In some embodiments provided is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:196) includes one or more 2′OMe modified pyrimidines; a 3′-terminal nucleotide or non-nucleotide overhang; and a cap moiety covalently attached at the 5′-terminus. In some embodiments the antisense strand (SEQ ID NO:221) includes one or more 2′OMe modified pyrimidines; a nucleotide or non-nucleotide moiety covalently attached at the 3′-terminus; and a cap moiety covalently attached at the 5′-terminus.

In some embodiments provided is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:196) includes 2′OMe modified ribonucleotides in positions 2, 14 and 18; a C3OH moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:221) is selected from an antisense strand which includes

-   -   a) 2′OMe modified ribonucleotides in positions 3, 5, 9, 11, 13,         15 and 17; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH         moiety covalently attached to the 3′-terminus; or     -   b) 2′OMe modified ribonucleotides in positions 3, 5, 7, 9, 12,         13 and 17; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH         moiety covalently attached to the 3′-terminus

In some embodiments provided is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:196) includes 2′OMe modified ribonucleotides in positions 2, 14 and 18; a C3-OH moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:221) includes 2′OMe modified ribonucleotides in positions 3, 5, 9, 11, 13, 15 and 17; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH moiety covalently attached to the 3′-terminus

In some embodiments provided is a duplex oligonucleotide molecule wherein the sense strand (SEQ ID NO:196) includes 2′OMe modified ribonucleotides in positions 14 and 18 and optionally in position 2; a C3-OH moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:221) includes 2′OMe modified ribonucleotides in positions 3, 5, 7, 9, 12, 13, and 17; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH moiety covalently attached to the 3′-terminus

In some embodiments provided is a duplex oligonucleotide molecule wherein the sense strand (SEQ ID NO:196) includes 2′OMe modified ribonucleotides in positions 14 and 18; a C3-OH moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:221) is selected from an antisense strand which includes

-   -   a) 2′OMe modified ribonucleotides in positions 3, 5, 9, 11, 13,         15 and 17; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH         moiety covalently attached to the 3′-terminus; or     -   b) 2′OMe modified ribonucleotides in positions 3, 5, 7, 9, 12,         13 and 17; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH         moiety covalently attached to the 3′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:196) includes 2′OMe modified ribonucleotides in positions 14 and 18; a C3-OH moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:220) includes 2′OMe modified ribonucleotides in positions 1, 3, 5, 9, 11, 13, 15 and 17; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH moiety covalently attached to the 3′-terminus

Provided herein is a double-stranded nucleic acid molecule wherein the sense strand (SEQ ID NO:196) includes 2′OMe modified ribonucleotides in positions 14 and 18; a C3-OH moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand (SEQ ID NO:220) includes 2′OMe modified ribonucleotides in positions 1, 3, 5, 7, 9, 12, 13, and 17; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH moiety covalently attached to the 3′-terminus

In further embodiments of Structures A1 and A2, (N′)y includes 1-8 modified ribonucleotides wherein the modified ribonucleotide is a DNA nucleotide. In certain embodiments (N′)_(y) includes 1, 2, 3, 4, 5, 6, 7, or up to 8 DNA moieties.

In some embodiments, either Z or Z′ is present and independently includes two non-nucleotide moieties.

In additional embodiments, Z and Z′ are present and each independently includes two non-nucleotide moieties.

In some embodiments, each of Z and Z′ includes an abasic moiety, for example a deoxyribo-abasic moiety (referred to herein as “dAb”) or ribo-abasic moiety (referred to herein as “rAb”). In some embodiments each of Z and/or Z′ includes two covalently linked abasic moieties and is for example dAb-dAb or rAb-rAb or dAb-rAb or rAb-dAb, wherein each moiety is covalently attached to an adjacent moiety, preferably via a phospho-based bond. In some embodiments the phospho-based bond includes a phosphorothioate, a phosphonoacetate or a phosphodiester bond. In preferred embodiments the phospho-based bond includes a phosphodiester bond.

In some embodiments, each of Z and/or Z′ independently includes an alkyl moiety, optionally propane ((CH₂)₃) moiety (“C3”) or a derivative thereof including propanediol (C3OH) and phospho derivative of propanediol (“C3Pi”). In some embodiments each of Z and/or Z′ includes two alkyl moieties covalently linked to the 3′-terminus of the antisense strand or sense strand via a phosphodiester or phosphorothioate linkage and covalently linked to one another via a phosphodiester or phosphorothioate linkage and in some examples is C3Pi-C3Pi or C3Pi-C3OH. The 3′-terminus of the antisense strand and/or the 3′-terminus of the sense strand is covalently attached to a C3 moiety via a phospho-based bond and the C3 moiety is covalently conjugated a C3OH moiety via a phospho-based bond. In some embodiments the phospho-based bonds include a phosphorothioate, a phosphonoacetate or a phosphodiester bond. In preferred embodiments the phospho-based bond includes a phosphodiester bond.

In various embodiments of Structure A1 or Structure A2, Z and Z′ are absent. In other embodiments Z or Z′ is present. In some embodiments, each of Z and/or Z′ independently includes a C2, C3, C4, C5 or C6 alkyl moiety, optionally a C3 moiety or a derivative thereof including propanol (C3OH/C3OH), propanediol, and phosphodiester derivative of propanediol (C3Pi). In preferred embodiments, each of Z and/or Z′ includes two hydrocarbon moieties and in some examples is C3Pi-C3OH or C3Pi-C3Pi. Each C3 is covalently conjugated to an adjacent C3 via a covalent bond, preferably a phospho-based bond. In some embodiments, the phospho-based bond is a phosphorothioate, a phosphonoacetate or a phosphodiester bond.

In specific embodiments, x=y=19 and Z comprises at least one C3 alkyl overhang. In some embodiments the C3-C3 overhang is covalently attached to the 3′-terminus of (N)_(x) or (N′)_(y) via a covalent linkage, preferably a phosphodiester linkage. In some embodiments, the linkage between a first C3 and a second C3 is a phosphodiester linkage. In some embodiments, the 3′ non-nucleotide overhang is C3Pi-C3Pi. In some embodiments the 3′ non-nucleotide overhang is C3Pi-C3Pi. In some embodiments the 3′ non-nucleotide overhang is C3Pi-C3OH.

In various embodiments, the alkyl moiety comprises an alkyl derivative including a C3 alkyl, C4 alkyl, C5 alky or C6 alkyl moiety comprising a terminal hydroxyl, a terminal amino, or terminal phosphate group. In some embodiments, the alkyl moiety is a C3 alkyl or C3 alkyl derivative moiety. In some embodiments, the C3 alkyl moiety comprises propanol, propylphosphate, propylphosphorothioate or a combination thereof. The C3 alkyl moiety is covalently linked to the 3′-terminus of (N′)_(y) and/or the 3′-terminus of (N)_(x) via a phosphodiester bond. In some embodiments, the alkyl moiety comprises propanol, propyl phosphate or propyl phosphorothioate. In some embodiments, each of Z and Z′ is independently selected from propanol, propyl phosphate propyl phosphorothioate, combinations thereof or multiples thereof in particular 2 or 3 covalently linked propanol, propyl phosphate, propyl phosphorothioate or combinations thereof. In some embodiments, each of Z and Z′ is independently selected from propyl phosphate, propyl phosphorothioate, propyl phospho-propanol; propyl phospho-propyl phosphorothioate; propylphospho-propyl phosphate; (propyl phosphate)₃, (propyl phosphate)₂-propanol, (propyl phosphate)₂-propyl phosphorothioate. Any propane or propanol conjugated moiety can be included in Z or Z′.

The structures of exemplary 3′-terminal non-nucleotide moieties are as follows:

In some embodiments, each of Z and Z′ is independently selected from propanol, propyl phosphate, propyl phosphorothioate, combinations thereof or multiples thereof

In some embodiments, each of Z and Z′ is independently selected from propyl phosphate, propyl phosphorothioate, propyl phospho-propanol; propyl phospho-propyl phosphorothioate; propylphospho-propyl phosphate; (propyl phosphate)₃, (propyl phosphate)₂-propanol, (propyl phosphate)₂-propyl phosphorothioate. Any propane or propanol conjugated moiety can be included in Z or Z′.

In additional embodiments, each of Z and/or Z′ includes a combination of an abasic moiety and an unmodified deoxyribonucleotide or ribonucleotide or a combination of a hydrocarbon moiety and an unmodified deoxyribonucleotide or ribonucleotide or a combination of an abasic moiety (deoxyribo or ribo) and a hydrocarbon moiety. In such embodiments, each of Z and/or Z′ includes C3-rAb or C3-dAb wherein each moiety is covalently bond to the adjacent moiety via a phospho-based bond, preferably a phosphodiester, phosphorothioate or phosphonoacetate bond.

In certain embodiments, nucleic acid molecules as disclosed herein include a sense oligonucleotide sequence selected from any one of oligonucleotide shown infra in Tables 4 and 5 (SEQ ID NOS:60-126 and 194-218).

In certain preferred embodiments, compounds provided include Compound_(—)1, Compound_(—)2, Compound_(—)3, Compound_(—)4, Compound_(—)5, Compound_(—)6, Compound_(—)7, Compound_(—)8, and Compound_(—)9, described infra.

In some embodiments, (such as, for example, Compound_(—)1, Compound_(—)5, and Compound_(—)6) provided are 19-mer double-stranded nucleic acid molecules wherein the antisense strand is SEQ ID NO:127 and the sense strand is SEQ ID NO:60. In certain embodiments, provided are 19-mer double-stranded nucleic acid molecules wherein the antisense strand is SEQ ID NO:127 and includes 2′OMe modified ribonucleotides; a 2′-5′-ribonucleotide in at least one of positions 1, 5, 6, or 7; and a non-nucleotide moiety covalently attached to the 3′-terminus; and the sense strand is SEQ ID NO:60 and includes at least one 2′-5′-ribonucleotide or 2′OMe modified ribonucleotide; a non-nucleotide moiety covalently attached at the 3′-terminus; and a cap moiety covalently attached at the 5′-terminus. In some embodiments, provided are 19-mer double-stranded nucleic acid molecule wherein the antisense strand is SEQ ID NO:127; and includes 2′OMe modified ribonucleotides at positions 3, 5, 9, 11, 13, 15, 17, and 19; a 2′-5′-ribonucleotide in position 7; and a C3OH non-nucleotide moiety covalently attached at the 3′-terminus; and the sense strand is SEQ ID NO:60 and includes five consecutive 2′-5′-ribonucleotides in the 3′-terminal positions 15, 16, 17, 18, and 19; a C3Pi non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic moiety covalently attached at the 5′-terminus.

In one embodiment, provided is Compound_(—)1 that is a 19-mer double-stranded nucleic acid molecule wherein the antisense strand is SEQ ID NO:127 and includes 2′OMe modified ribonucleotides at positions 3, 5, 9, 11, 13, 15, 17, and 19; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH non-nucleotide moiety covalently attached at the 3′-terminus; and the sense strand is SEQ ID NO:60 and includes five consecutive 2′-5′-ribonucleotides in the 3′-terminal positions 15, 16, 17, 18, and 19; a C3Pi non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic moiety covalently attached at the 5′-terminus; and that further includes a 2′OMe modified ribonucleotide at position 1 of the antisense strand.

In one embodiment, provided is Compound_(—)6 that is a 19-mer double-stranded nucleic acid molecule wherein the antisense strand is SEQ ID NO:127 and includes 2′OMe modified ribonucleotides at positions 3, 5, 9, 11, 13, 15, 17, and 19; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH non-nucleotide moiety covalently attached at the 3′-terminus; and the sense strand is SEQ ID NO:60 and includes five consecutive 2′-5′-ribonucleotides in the 3′-terminal positions 15, 16, 17, 18, and 19; a C3Pi non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic moiety covalently attached at the 5′-terminus; and that further includes a 2′-5′-ribonucleotide at position 1 of the antisense strand.

In one embodiment, provided is Compound_(—)5 that is a 19-mer double-stranded nucleic acid molecule wherein the antisense strand is SEQ ID NO:127 and includes 2′OMe modified ribonucleotides in positions 1, 3, 5, 9, 11, 13, 15, 17, and 19; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH non-nucleotide moiety covalently attached at the 3′-terminus; and the sense strand is SEQ ID NO:60 and includes 2′OMe modified ribonucleotides in positions 7, 13, 16 and 18; a 2′-5′-ribonucleotide at position 9; a C3OH non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic moiety covalently attached at the 5′-terminus

In some embodiments, (such as, for example, Compound_(—)2, and Compound_(—)7, described infra) provided are 19-mer double-stranded nucleic acid molecules wherein the sense strand is SEQ ID NO:63 and the antisense strand is SEQ ID NO:130. In some embodiments provided are 19-mer double-stranded nucleic acid molecules wherein the sense strand is SEQ ID NO:63 and includes 2′OMe modified pyrimidine ribonucleotides; a non-nucleotide moiety covalently attached at the 3′-terminus; and a cap moiety covalently attached at the 5′-terminus; and the antisense strand is SEQ ID NO:130 and includes 2′OMe modified ribonucleotides; a 2′-5′-ribonucleotide at position 7; and a non-nucleotide moiety covalently attached at the 3′-terminus. In some embodiments provided are 19-mer double-stranded nucleic acid molecules wherein the sense strand is SEQ ID NO:63 and includes 2′OMe modified ribonucleotides; a non-nucleotide moiety covalently attached at the 3′-terminus; and a cap moiety covalently attached at the 5′-terminus; and the antisense strand is SEQ ID NO:130 and includes 2′OMe modified ribonucleotides; a 2′-5′-ribonucleotide in at least one of positions 5, 6 or 7; and a non-nucleotide moiety covalently attached at the 3′-terminus

In one embodiment, provided is Compound_(—)2 that is a 19-mer double-stranded nucleic acid molecule wherein the sense strand is SEQ ID NO:63 and includes 2′OMe modified ribonucleotides in positions 2, 14 and 18; a C3OH moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand is SEQ ID NO:130 and includes 2′OMe modified ribonucleotides in positions 1, 3, 5, 9, 12, 13, and 17; a 2′-5′-ribonucleotide in at least one of positions 5, 6 or 7; and C3Pi-C3OH non-nucleotide moiety covalently attached at the 3′-terminus

In one embodiment, provided is Compound_(—)7 that is a 19-mer double-stranded nucleic acid molecule wherein the sense strand is SEQ ID NO:63 and includes 2′OMe modified ribonucleotides in positions 2, 14 and 18; a C3OH moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand is SEQ ID NO:130 and includes 2′OMe modified ribonucleotides in positions 1, 3, 5, 9, 11, 13, and 17; a 2′-5′-ribonucleotide at position 7; and a C3Pi-C3OH non-nucleotide moiety covalently attached at the 3′-terminus

In some embodiments, (such as, for example, Compound_(—)3, described infra) provided are 19-mer double-stranded nucleic acid molecules wherein the sense strand is SEQ ID NO:98 and the antisense strand is SEQ ID NO:165. In some embodiments, provided are 19-mer double-stranded nucleic acid molecules wherein the sense strand is SEQ ID NO:98 and includes 2′-5′-ribonucleotides in positions at the 3′-terminus; a non-nucleotide moiety covalently attached at the 3′-terminus; and a cap moiety covalently attached at the 5′-terminus; and the antisense strand is SEQ ID NO:165 and includes 2′OMe modified ribonucleotides; a 2′-5′-ribonucleotide in at least one of positions 5, 6 or 7; and a non-nucleotide moiety covalently attached at the 3′-terminus. In one embodiment, provided is Compound_(—)3 that is a 19-mer double-stranded nucleic acid molecule wherein the sense strand is SEQ ID NO:98 and includes 2′-5′-ribonucleotides in positions 15, 16, 17, 18, and 19; a C3-OH 3′ moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand is SEQ ID NO:165 and includes 2′OMe modified ribonucleotides in positions 2, 4, 6, 8, 11, 13, 15, 17, and 19; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH covalently attached at the 3′-terminus.

In some embodiments, (such as, for example, Compound_(—)4, Compound_(—)8 and Compound_(—)9, described infra) provided are 19-mer double-stranded nucleic acid molecules wherein the sense strand is SEQ ID NO:101 and the antisense strand is SEQ ID NO:168. In some embodiments provided are 19-mer double-stranded nucleic acid molecules wherein the sense strand is SEQ ID NO:101 and includes 2′OMe modified pyrimidine ribonucleotides; an optional 2′-5′-ribonucleotide in one of position 9 or 10; a non-nucleotide moiety covalently attached at the 3′-terminus; and a cap moiety covalently attached at the 5′-terminus; and the antisense strand is SEQ ID NO:168 and includes 2′OMe modified ribonucleotides; a 2′-5′-ribonucleotide in at least one of positions 5, 6, or 7; and a non-nucleotide moiety covalently attached at the 3′-terminus

In one embodiment, provided is Compound_(—)4 that is a 19-mer double-stranded nucleic acid molecule wherein sense strand is SEQ ID NO:101 and includes 2′OMe modified ribonucleotides in positions 4, 11, 13, and 17; a 2′-5′-ribonucleotide in position 9; a C3OH non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand is SEQ ID NO:168 and includes 2′OMe modified ribonucleotides in positions 1, 4, 8, 11 and 15; a 2′-5′-ribonucleotide in position 6; a C3Pi-C3OH overhang covalently attached at the 3′-terminus

In one embodiment, provided is Compound_(—)8 that is a 19-mer double-stranded nucleic acid molecule wherein sense strand is SEQ ID NO:101 and includes 2′OMe modified ribonucleotides in positions 4, 11, 13, and 17; a C3OH non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand is SEQ ID NO:168 and includes 2′OMe modified ribonucleotides in positions 1, 4, 8, 13 and 15; a 2′-5′-ribonucleotide in position 6; and a C3Pi-C3OH overhang covalently attached at the 3′-terminus

In one embodiment, provided is Compound_(—)9 that is a 19-mer double-stranded nucleic acid molecule wherein the sense strand is SEQ ID NO:101 and includes 2′OMe modified ribonucleotides in positions 2, 4, 11, 13, and 17; a C3OH non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand is SEQ ID NO:168 and includes 2′OMe modified ribonucleotides in positions 1, 4, 8, 11 and 15; a 2′-5′-ribonucleotide in position 6; and a C3Pi-C3OH moiety covalently attached at the 3′-terminus

In another aspect, provided are methods for reducing the expression of hsp47 in a cell by introducing into a cell a nucleic acid molecule as provided herein in an amount sufficient to reduce expression of hsp47. In one embodiment, the cell is hepatocellular stellate cell. In another embodiment, the cell is a stellate cell in renal or pulmonary tissue. In certain embodiment, the method is performed in vitro. In another embodiment, the method is performed in vivo.

In another aspect, provided are methods for treating an individual suffering from a disease associated with hsp47. The methods include administering to the individual a nucleic acid molecule such as provided herein in an amount sufficient to reduce expression of hsp47. In certain embodiments, the disease associated with hsp47 is a disease selected from the group consisting of liver fibrosis, cirrhosis, pulmonary fibrosis including lung fibrosis (including ILF), any condition causing kidney fibrosis (e.g., CKD including ESRD), peritoneal fibrosis, chronic hepatic damage, fibrillogenesis; fibrotic diseases in other organs; abnormal scarring (keloids) associated with all possible types of skin injury, accidental and iatrogenic (operations), scleroderma, cardiofibrosis, failure of glaucoma filtering operation, and intestinal adhesions. In some embodiments, the compounds may be useful in treating organ-specific indications, for example, indications including those shown in Table 1 below, listing organs and respective indications:

TABLE 1 Skin Pathologic scarring as keloid and hypertrophic scar Surgical scarring Injury scarring keloid, or nephrogenic fibrosing dermatopathy Peritoneum Peritoneal fibrosis Adhesions Peritoneal Sclerosis associated with continual ambulatory peritoneal dialysis (CAPD) Liver Cirrhosis including post-hepatitis C cirrhosis, primary biliary cirrhosis Liver fibrosis, e.g. Prevention of Liver Fibrosis in Hepatitis C carriers schistomasomiasis cholangitis Liver cirrhosis due to Hepatitis C post liver transplant or Non-Alcoholic Steatohepatitis (NASH) Pancreas inter(peri)lobular fibrosis (e.g., alcoholic chronic pancreatitis), periductal fibrosis (e.g., hereditary pancreatitis), periductal and interlobular fibrosis (e.g., autoimmune pancreatitis), diffuse inter- and intralobular fibrosis (e.g., obstructive chronic pancreatitis) Kidney Chronic Kidney Disease (CKD) of any etiology. Treatment of early stage CKD (elevated SCr) in diabetic patients (prevent further deterioration in renal function) kidney fibrosis associated with lupus glomeruloschelerosis Diabetic Nephropathy Heart Congestive heart failure, Endomyocardial fibrosis, cardiofibrosis fibrosis associated with myocardial infarction Lung Asthma, Idiopathic pulmonary fibrosis (IPF); Interstitial lung fibrosis (ILF) Radiation Pneumonitis leading to Pulmonary Fibrosis (e.g. due to cancer treating radiation) Bone marrow Myeloproliferative disorders: Myelofibrosis (MF), Polycythemia vera (PV), Essential thrombocythemia (ET) idiopathic myelofibrosis drug induced myelofibrosis. Eye Anterior segment: Corneal opacification e,g, following inherited dystrophies, herpetic keratitis or pterygia; Glaucoma Posterior segment fibrosis and traction retinal detachment, a complication of advanced diabetic retinopathy (DR); Fibrovascular scarring and gliosis in the retina; Under the retina fibrosis for example subsequent to subretinal hemorrhage associated with neovascular AMD Retro-orbital fibrosis, postcataract surgery, proliferative vitreoretinopathy. Ocular cicatricial pemphigoid Intestine Intestinal fibrosis, Crohn's disease Vocal cord Vocal cord scarring, vocal cord mucosal fibrosis, laryngeal fibrosis Vasculature Atherosclerosis, postangioplasty arterial restenosis Multisystemic Scleroderma systemic sclerosis; multifocal fibrosclerosis; sclerodermatous graft-versus-host disease in bone marrow transplant recipients, and nephrogenic systemic fibrosis (exposure to gadolinium-based contrast agents (GBCAs), 30% of MRIs) Malignancies of various origin Metastatic and invasive cancer by inhibiting function of activated tumor associated myofibroblasts

Another embodiment of the description is a method for treating a stellate cell-related disorder, the method comprising administering an effective amount of the pharmaceutical composition described infra to a subject in need thereof. The disorder includes hepatitis, hepatic fibrosis, hepatic cirrhosis, liver cancer, pancreatitis, pancreatic fibrosis, pancreatic cancer, vocal cord scarring, vocal cord mucosal fibrosis, and laryngeal fibrosis. In some embodiments the preferred indications include, liver cirrhosis due to Hepatitis C post liver transplant; liver cirrhosis due to Non-Alcoholic Steatohepatitis (NASH); idiopathic pulmonary fibrosis; radiation pneumonitis leading to pulmonary fibrosis; diabetic nephropathy; peritoneal sclerosis associated with continual ambulatory peritoneal dialysis (CAPD) and ocular cicatricial pemphigoid.

Fibrotic liver indications include Alcoholic Cirrhosis, Hepatitis B cirrhosis, Hepatitis C cirrhosis, Hepatitis C (Hep C) cirrhosis post-orthotopic liver transplant (OLTX), non-alcoholic steatohepatitis/nonalcoholic fatty liver disease (NASH/NAFLD), primary biliary cirrhosis (PBC), primary sclerosing cholangitis (PSC), biliary atresia, alpha-1 antitrypsin deficiency (A1AD), copper storage diseases (Wilson's disease), fructosemia, galactosemia, glycogen storage diseases (especially types III, IV, VI, IX, and X), iron-overload syndromes (hemochromatosis), lipid abnormalities (e.g., Gaucher's disease), peroxisomal disorders (e.g., Zellweger syndrome), tyrosinemia, congenital hepatic fibrosis, bacterial Infections (e.g., brucellosis), parasitic (e.g., echinococcosis), Budd-Chiari syndrome (hepatic veno-occlusive disease).

Pulmonary indications include Idiopathic Pulmonary Fibrosis, Silicosis, Pneumoconiosis, bronchopulmonary dysplasia in newborn following neonatal respiratory distress syndrome, bleomycin/chemolung injury, brochiolitis obliterans (BOS) Post-lung transplant, Chronic obstructive pulmonary disorder (COPD), cystic fibrosis, and asthma.

Cardiac indications include cardiomyopathy, atherosclerosis (Bergers disease, etc), endomyocardial fibrosis, atrial fibrillation, scarring post-myocardial infarction (MI)

Other Thoracic indications include Radiation-induced capsule tissue reactions around textured breast implants, and oral submucosal fibrosis.

Renal indications include autosomal dominant polycystic kidney disease (ADPKD), Diabetic nephropathy (diabetic glomerulosclerosis), focal segmental glomerulosclerosis (FSGS) (collapsing vs. other histologic variants), IgA Nephropathy (Berger Disease), Lupus Nephritis, Wegner's, Scleroderma, Goodpasture Syndrome, tubulointerstitial fibrosis: drug induced (protective) pencillins, cephalosporins, analgesic nephropathy, membrano-proliferative glomerulonephritis (MPGN), Henoch-Schonlein purpura, Congenital nephropathies: Medullary Cystic Disease, Nail-Patella Syndrome and Alport Syndrome.

Bone Marrow indications include lymphangiolyomyositosis (LAM), chronic graft vs. host disease, polycythemia vera, essential thrombocythemia, and myelofibrosis.

Ocular indications include retinopathy of prematurity (RoP), ocular cicatricial pemphigoid, Lacrimal gland fibrosis, Retinal attachment surgery, corneal opacity, herpetic keratitis, pterygia, Glaucoma, age-related macular degeneration (AMD/ARMD), retinal fibrosis associated diabetes mellitus (DM) retinopathy

Gynecological indications include Endometriosis add on to hormonal therapy for prevention of scarring, post-STD fibrosis/salphingitis,

Systemic indications include Dupuytren's disease, Palmar fibromatosis, Peyronie's disease, Ledderhose disease, keloids, multifocal fibrosclerosis, nephrogenic systemic fibrosis and myelofibrosis (anemia).

Injury-associated fibrotic diseases include burn-induced (chemical included) skin and soft tissue scarring and contraction, radiation-induced skin and organ scarring, post cancer therapeutic radiation treatment, keloid (skin).

Surgical indications include peritoneal fibrosis post-peritoneal dialysis catheter, corneal implant, cochlear implant, other implants, silicone implants in breasts, chronic sinusitis; adhesions, pseudointimal hyperplasia of dialysis grafts.

Other indications include chronic pancreatitis.

In some embodiments, provided is a method for treatment of a subject suffering from liver fibrosis comprising administering to the subject an effective amount of a nucleic acid molecule disclosed herein, thereby treating liver fibrosis. In some embodiments the subject is suffering from cirrhosis of the liver due to hepatitis. In some embodiments the subject is suffering from cirrhosis of the liver due to NASH.

In some embodiments, provided is the use of a nucleic acid molecule disclosed herein for the manufacture of a medicament to treat liver fibrosis. In some embodiments, the liver fibrosis is due to hepatitis. In some embodiments, the liver fibrosis is due to NASH.

In some embodiments, provided is a method for remodeling of scar tissue comprising administering to a subject in need thereof an effective amount of a nucleic acid molecule disclosed herein, thereby effecting scar tissue remodeling. In some embodiments, the scar tissue is in the liver. In some embodiments, the subject is suffering from cirrhosis of the liver due to hepatitis. In some embodiments, the subject is suffering from cirrhosis of the liver due to NASH.

In some embodiments, a method for modulating fibrosis regression is provided comprising administering to a subject in need thereof an effective amount of a nucleic acid molecule disclosed herein, thereby effecting fibrosis regression.

In some embodiments, provided is a method for reduction of scar tissue in a subject comprising the step of administering to the subject an effective amount of a nucleic acid molecule disclosed herein to reduce the scar tissue. In some embodiments, provided is a method for reducing scar tissue in a subject comprising the step of topically applying to scar tissue an effective amount of a nucleic acid molecule disclosed herein to reduce scar tissue.

In some embodiments, provided is a method for improving the appearance of scar tissue comprising the step of topically applying to scar tissue an effective amount of a nucleic acid molecule disclosed herein to improve the appearance of the scar tissue.

In some embodiments, provided is a method for treatment of a subject suffering from lung fibrosis comprising administering to the subject an effective amount of a nucleic acid molecule disclosed herein, thereby treating the lung fibrosis. In some embodiments, the subject is suffering from interstitial lung fibrosis (ILF). In some embodiments, the subject is suffering from Radiation Pneumonitis leading to Pulmonary Fibrosis. In some embodiments, the subject is suffering from drug induced lung fibrosis.

In some embodiments, provided is the use of a nucleic acid molecule disclosed herein for the manufacture of a medicament to treat lung fibrosis. In some embodiments, the lung fibrosis is ILF. In some embodiments, the lung fibrosis drug- or radiation-induced lung fibrosis.

In one aspect, provided are pharmaceutical compositions that include a nucleic acid molecule (e.g., a siNA molecule) as described herein in a pharmaceutically acceptable carrier. In certain embodiments, the pharmaceutical formulation includes, or involves, a delivery system suitable for delivering nucleic acid molecules (e.g., siNA molecules) to an individual such as a patient; for example delivery systems described in more detail below.

In a related aspect, provided are compositions or kits that include a nucleic acid molecule (e.g., an siNA molecule) packaged for use by a patient. The package may be labeled or include a package label or insert that indicates the content of the package and provides certain information regarding how the nucleic acid molecule (e.g., an siNA molecule) should be or can be used by a patient, for example the label may include dosing information and/or indications for use. In certain embodiments, the contents of the label will bear a notice in a form prescribed by a government agency, for example the United States Food and Drug administration (FDA). In certain embodiments, the label may indicate that the nucleic acid molecule (e.g., an siNA molecule) is suitable for use in treating a patient suffering from a disease associated with hsp47; for example, the label may indicate that the nucleic acid molecule (e.g., an siNA molecule) is suitable for use in treating fibroids; or for example the label may indicate that the nucleic acid molecule (e.g., an siNA molecule) is suitable for use in treating a disease selected from the group consisting of fibrosis, liver fibrosis, cirrhosis, pulmonary fibrosis, kidney fibrosis, peritoneal fibrosis, chronic hepatic damage, and fibrillogenesis.

As used herein, the term “heat shock protein 47” or “hsp47” or “HSP47” are used interchangeably and refer to any heat shock protein 47, peptide, or polypeptide having any hsp47 protein activity. Heat shock protein 47 is a serine proteinase inhibitor (serpin) also known, for example, as serpin peptidase inhibitor, clade H, member 1 (SERPINH1), SERPINH2, collagen binding protein 1 (CBP1), CBP2, gp46; arsenic-transactivated protein 3 (AsTP3); HSP47; proliferation-inducing gene 14 (PIG14); PPROM; rheumatoid arthritis antigen A-47 (RA-A47); colligin-1; and colligin-2. In certain preferred embodiments, “hsp47” refers to human hsp47. Heat shock protein 47 (or more particularly human hsp47) may have an amino acid sequence that is the same, or substantially the same, as SEQ ID NO. 2.

As used herein the term “nucleotide sequence encoding hsp47” means a nucleotide sequence that codes for an hsp47 protein, or portion thereof. The term “nucleotide sequence encoding hsp47” is also meant to include hsp47 coding sequences such as hsp47 isoforms, mutant hsp47 genes, splice variants of hsp47 genes, and hsp47 gene polymorphisms. A nucleic acid sequence encoding hsp47 includes mRNA sequences encoding hsp47, which can also be referred to as “hsp47 mRNA.” An exemplary sequence of human hsp47 mRNA is SEQ ID. NO. 1.

As used herein, the term “nucleic acid molecule” or “nucleic acid” are used interchangeably and refer to an oligonucleotide, nucleotide or polynucleotide. Variations of “nucleic acid molecule” are described in more detail herein. A nucleic acid molecule encompasses both modified nucleic acid molecules and unmodified nucleic acid molecules as described herein. A nucleic acid molecule may include deoxyribonucleotides, ribonucleotides, modified nucleotides or nucleotide analogs in any combination.

As used herein, the term “nucleotide” refers to a chemical moiety having a sugar (or an analog thereof, or a modified sugar), a nucleotide base (or an analog thereof, or a modified base), and a phosphate group (or analog thereof, or a modified phosphate group). A nucleotide encompasses modified nucleotides or unmodified nucleotides as described herein. As used herein, nucleotides may include deoxyribonucleotides (e.g., unmodified deoxyribonucleotides), ribonucleotides (e.g., unmodified ribonucleotides), and modified nucleotide analogs including, inter alia, LNA and UNA, peptide nucleic acids, L-nucleotides (also referred to as mirror nucleotides), ethylene-bridged nucleic acid (ENA), arabinoside, PACE, nucleotides with a 6 carbon sugar, as well as nucleotide analogs (including abasic nucleotides) often considered to be non-nucleotides. In some embodiments, nucleotides may be modified in the sugar, nucleotide base and/or in the phosphate group with any modification known in the art, such as modifications described herein. A “polynucleotide” or “oligonucleotide” as used herein, refers to a chain of linked nucleotides; polynucleotides and oligonucleotides may likewise have modifications in the nucleotide sugar, nucleotide bases and phosphate backbones as are well known in the art and/or are disclosed herein.

As used herein, the term “short interfering nucleic acid”, “siNA”, or “short interfering nucleic acid molecule” refers to any nucleic acid molecule capable of modulating gene expression or viral replication. Preferably siNA inhibits or down regulates gene expression or viral replication. siNA includes without limitation nucleic acid molecules that are capable of mediating sequence specific RNAi, for example short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), short hairpin RNA (shRNA), short interfering oligonucleotide, short interfering nucleic acid, short interfering modified oligonucleotide, chemically-modified siRNA, post-transcriptional gene silencing RNA (ptgsRNA), and others. As used herein, “short interfering nucleic acid”, “siNA”, or “short interfering nucleic acid molecule” has the meaning described in more detail elsewhere herein.

As used herein, the term “complementary” means that a nucleic acid can form hydrogen bond(s) with another nucleic acid sequence by either traditional Watson-Crick or other non-traditional types. In reference to the nucleic molecules disclosed herein, the binding free energy for a nucleic acid molecule with its complementary sequence is sufficient to allow the relevant function of the nucleic acid to proceed, e.g., RNAi activity. Determination of binding free energies for nucleic acid molecules is well known in the art (see, e.g., Turner et al., 1987, CSH Symp. Quant. Biol. LII pp. 123-133; Frier et al., 1986, Proc. Nat. Acad. Sci. USA 83:9373-9377; Turner et al., 1987, J. Am. Chem. Soc. 109:3783-3785). A percent complementarity indicates the percentage of contiguous residues in a nucleic acid molecule that can form hydrogen bonds (e.g., Watson-Crick base pairing) with a second nucleic acid sequence (e.g., 5, 6, 7, 8, 9, or 10 nucleotides out of a total of 10 nucleotides in the first oligonucleotide being based paired to a second nucleic acid sequence having 10 nucleotides represents 50%, 60%, 70%, 80%, 90%, and 100% complementary respectively). “Fully complementary” means that all the contiguous residues of a nucleic acid sequence will hydrogen bond with the same number of contiguous residues in a second nucleic acid sequence. In one embodiment, a nucleic acid molecule disclosed herein includes about 15 to about 35 or more (e.g., about 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34 or 35 or more) nucleotides that are complementary to one or more target nucleic acid molecules or a portion thereof.

As used herein, the term “sense region” refers to a nucleotide sequence of a siNA molecule complementary (partially or fully) to an antisense region of the siNA molecule. The sense strand of a siNA molecule can include a nucleic acid sequence having homology with a target nucleic acid sequence. As used herein, “sense strand” refers to nucleic acid molecule that includes a sense region and may also include additional nucleotides. Nucleotide positions of the sense strand are herein numbered 5′>3′.

As used herein, the term “antisense region” refers to a nucleotide sequence of a siNA molecule complementary (partially or fully) to a target nucleic acid sequence. The antisense strand of a siNA molecule can optionally include a nucleic acid sequence complementary to a sense region of the siNA molecule. As used herein, “antisense strand” refers to nucleic acid molecule that includes an antisense region and may also include additional nucleotides. Nucleotide positions of the antisense strand are herein numbered 5′>3′.

As used herein, the term “RNA” refers to a molecule that includes at least one ribonucleotide residue.

As used herein, the term “duplex region” refers to the region in two complementary or substantially complementary oligonucleotides that form base pairs with one another, either by Watson-Crick base pairing or any other manner that allows for a duplex between oligonucleotide strands that are complementary or substantially complementary. For example, an oligonucleotide strand having 21 nucleotide units can base pair with another oligonucleotide of 21 nucleotide units, yet only 19 bases on each strand are complementary or substantially complementary, such that the “duplex region” consists of 19 base pairs. The remaining base pairs may, for example, exist as 5′ and 3′ overhangs. Further, within the duplex region, 100% complementarity is not required; substantial complementarity is allowable within a duplex region. Substantial complementarity refers to complementarity between the strands such that they are capable of annealing under biological conditions. Techniques to empirically determine if two strands are capable of annealing under biological conditions are well known in the art. Alternatively, two strands can be synthesized and added together under biological conditions to determine if they anneal to one another.

As used herein, the terms “non-pairing nucleotide analog” means a nucleotide analog which includes a non-base pairing moiety including but not limited to: 6-des-amino adenosine (nebularine), 4-Me-indole, 3-nitropyrrole, 5-nitroindole, Ds, Pa, N3-Me ribo U, N3-Me riboT, N3-Me dC, N3-Me-dT, N1-Me-dG, N1-Me-dA, N3-ethyl-dC, N3-Me dC. In some embodiments the non-base pairing nucleotide analog is a ribonucleotide. In other embodiments it is a deoxyribonucleotide.

As used herein, the term, “terminal functional group” includes without limitation a halogen, alcohol, amine, carboxylic, ester, amide, aldehyde, ketone, ether groups.

An “abasic nucleotide” or “abasic nucleotide analog” is as used herein may also be often referred to herein and in the art as a pseudo-nucleotide or an unconventional moiety. While a nucleotide is a monomeric unit of nucleic acid, generally consisting of a ribose or deoxyribose sugar, a phosphate, and a base (adenine, guanine, thymine, or cytosine in DNA; adenine, guanine, uracil, or cytosine in RNA), an abasic or pseudo-nucleotide lacks a base, and thus is not strictly a nucleotide as the term is generally used in the art. Abasic deoxyribose moieties include for example, abasic deoxyribose-3′-phosphate; 1,2-dideoxy-D-ribofuranose-3-phosphate; 1,4-anhydro-2-deoxy-D-ribitol-3-phosphate. Inverted abasic deoxyribose moieties include inverted deoxyriboabasic; 3′,5′ inverted deoxyabasic 5′-phosphate.

The term “capping moiety” (z″) as used herein includes a moiety which can be covalently linked to the 5′-terminus of (N′)_(y) and includes abasic ribose moiety, abasic deoxyribose moiety, modifications abasic ribose and abasic deoxyribose moieties including 2′ O alkyl modifications; inverted abasic ribose and abasic deoxyribose moieties and modifications thereof; C₆-imino-Pi; a mirror nucleotide including L-DNA and L-RNA; 5′OMe nucleotide; and nucleotide analogs including 4′,5′-methylene nucleotide; 1-(β-D-erythrofuranosyl)nucleotide; 4′-thio nucleotide, carbocyclic nucleotide; 5′-amino-alkyl phosphate; 1,3-diamino-2-propyl phosphate, 3-aminopropyl phosphate; 6-aminohexyl phosphate; 12-aminododecyl phosphate; hydroxypropyl phosphate; 1,5-anhydrohexitol nucleotide; alpha-nucleotide; threo-pentofuranosyl nucleotide; acyclic 3′,4′-seco nucleotide; 3,4-dihydroxybutyl nucleotide; 3,5-dihydroxypentyl nucleotide, 5′-5′-inverted abasic moiety; 1,4-butanediol phosphate; 5′-amino; and bridging or non-bridging methylphosphonate and 5′-mercapto moieties.

Certain capping moieties may be abasic ribose or abasic deoxyribose moieties; inverted abasic ribose or abasic deoxyribose moieties; C6-amino-Pi; a mirror nucleotide including L-DNA and L-RNA. The nucleic acid molecules as disclosed herein may be synthesized using one or more inverted nucleotides, for example inverted thymidine or inverted adenine (for example see Takei, et al., 2002. JBC 277(26):23800-06).

The term “unconventional moiety” as used herein refers to non-nucleotide moieties including an abasic moiety, an inverted abasic moiety, a hydrocarbon (alkyl) moiety and derivatives thereof, and further includes a deoxyribonucleotide, a modified deoxyribonucleotide, a mirror nucleotide (L-DNA or L-RNA), a non-base pairing nucleotide analog and a nucleotide joined to an adjacent nucleotide by a 2′-5′ internucleotide phosphate bond; bridged nucleic acids including LNA and ethylene bridged nucleic acids, linkage modified (e.g. PACE) and base modified nucleotides as well as additional moieties explicitly disclosed herein as unconventional moieties.

As used herein, the term “inhibit”, “down-regulate”, or “reduce” with respect to gene expression means the expression of the gene, or level of RNA molecules or equivalent RNA molecules encoding one or more proteins or protein subunits (e.g., mRNA), or activity of one or more proteins or protein subunits, is reduced below that observed in the absence of an inhibitory factor (such as a nucleic acid molecule, e.g., an siNA, for example having structural features as described herein); for example the expression may be reduced to 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10%, 5% or less than that observed in the absence of an inhibitor.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a diagram showing a protocol with respect to assessment of the effect of gp46-siRNA in vitro using NRK cells, and determination of optimal sequence, timing, and concentration.

FIG. 2 is a photographic diagram showing the result of western blotting of gp46 and actin (24 hour culturing, examination of optimal sequence).

FIG. 3 is a photographic diagram showing the result of western blotting of gp46 and actin (24 hour culturing, examination of optimal concentration).

FIG. 4 is a photographic diagram showing the result of western blotting of gp46 and actin (concentration 50 nM, examination of optimal culturing time).

FIG. 5 is a diagram showing a protocol for evaluating inhibition of expression of collagen by gp46-siRNA in NRK cells.

FIG. 6 is a graph showing inhibition of collagen synthesis by siRNA.

FIG. 7 is a photographic diagram showing HSC-specific siRNA transfection.

FIG. 8 is a photographic diagram for evaluating HSC-specific siRNA transfection percentage.

FIG. 9 is a photographic diagram for evaluating inhibition of expression of gp46 by siRNA.

FIG. 10 is a photographic diagram showing azan staining of rat liver to which DMN had been administered.

FIG. 11 is a diagram showing an LC rat treatment protocol.

FIG. 12 is a photographic diagram showing azan staining of LC rat liver to which VA-Lip-gp46siRNA had been administered.

FIG. 13. is a diagram showing a method for extracting a stained portion by means of NIH Image (6 positions being randomly taken from an azan-stained image).

FIG. 14 is a graph showing the ratio by area occupied by fibrotic portions in liver histology (Collagen ratio by area, %).

FIG. 15 is a graph showing the amount of hydroxyproline in hepatic tissue.

FIG. 16 is a graph showing a survival curve for hepatic cirrhosis rat to which VA-Lip-gp46siRNA had been intraportally administered.

FIG. 17 is a photographic diagram showing azan staining of hepatic tissue of hepatic cirrhosis rat to which VA-Lip-gp46siRNA had been intraportally administered.

FIG. 18 is a graph showing a survival curve for hepatic cirrhosis rat to which VA-Lip-gp46siRNA had been intraportally administered.

FIG. 19 is a photographic diagram showing azan staining of hepatic tissue of hepatic cirrhosis rat to which VA-Lip-gp46siRNA had been intraportally administered.

FIG. 20 is a graph showing a survival curve for hepatic cirrhosis rat to which VA-Lip-gp46siRNA had been intravenously administered.

FIG. 21 is a graph showing a survival curve for hepatic cirrhosis rat to which VA-Lip-gp46siRNA had been intravenously administered.

FIG. 22 is a photographic diagram showing azan staining of hepatic tissue of hepatic cirrhosis rat to which VA-Lip-gp46siRNA had been intravenously administered.

FIG. 23 is a diagram showing improvement of VA-Lip-gp46siRNA transfection efficiency by RBP.

FIG. 24 is a diagram showing inhibition of VA-Lip-gp46siRNA transfection by anti-RBP antibody.

FIG. 25 is a bar graph showing the effect of GFP siNA on various reporter cell lines. Cell lines were established by lenti-viral induction of human HSP47 cDNA-GFP or rat GP46 cDNA-GFP construct into HEK293, human fibrosarcoma cell line HT1080, human HSC line hTERT or NRK cell line. Negative control siNA or siNA against GFP was introduced into the cells and GFP fluorescence was measured. The results showed that siNA against GFP knocks down the fluorescence to different extent in different cell lines. 293_HSP47-GFP and 293_GP46-GFP cell lines were selected for siHsp47 screening due to their easiness of being transfected and sensitivity to fluorescence knockdown.

FIGS. 26A, 26B, 26C, and 26D are a series of bar graphs showing the cytotoxicity and knockdown efficiency of various siHsp47s in 293_HSP47-GFP and 293_GP46-GFP cell lines. The result showed that siHsp47-C, siHsp47-2 and siHsp47-2d efficiently knockdown both human HSP47 and rat GP46 (the human hsp47 homolog) without substantial cytotoxicity. siGp46A against GP46 does not knock down human HSP47. Additionally, the newly designed siHsp47s outperformed siGp46A in knocking down rat GP46.

FIG. 27 is a bar graph showing the knock down effect of various siHsp47s on hsp47 mRNA, measured by TaqMan® qPCR using the human HSC cell line hTERT. The Y axis represents the remaining mRNA level of hsp47. HSP47-C was most effective among all the hsp47 siNAs tested.

FIG. 28 is a bar graph showing the effect of different hsp47 siNAs on collagen I expression in hTERT cells. The level of collagen I mRNA levels were measured by real-time quantitative PCR using TaqMan® probe. The Y axis represents the remaining mRNA expression level of collagen I. The result showed that collagen I mRNA level is significantly reduced in the cells treated with some of the candidates (siHsp47-2, siHsp47-2d, and their combination with siHsp47-1).

FIG. 29 is a graph showing a decrease in fibrotic areas of the liver in animals treated with siHSP47.

FIG. 30 is a schematic of the treatment schedule and evaluation method used in Example 22.

FIG. 31 shows the results of histological staining with Azan in the pulmonary field. The pictures display representative lung field of Azan-stained sections of each group at 80× magnification. (a) Pretreatment (BLM IT-2W); (b) Disease rat (BLM IT-5W+PBS i.v.); and (c) Treatment (BLM IT+siRNA i.v.), (d) Sham (Saline-IT+PBS i.v.).

FIG. 32 shows fibrosis scoring, showing the results of evaluating twenty randomly selected lung fields under 80× magnification for each rat. The bar graph summarizes the fibrosis scoring of Azan-stained section for each group. Statistical analysis were used One-way-ANOVA Bonferroni multi comparison test using Prism5 software.

DETAILED DESCRIPTION OF ILLUSTRATIVE EMBODIMENTS RNA Interference and siNA Nucleic Acid Molecules

RNA interference refers to the process of sequence-specific post-transcriptional gene silencing in animals mediated by short interfering RNAs (siRNAs) (Zamore et al., 2000, Cell, 101, 25-33; Fire et al., 1998, Nature, 391, 806; Hamilton et al., 1999, Science, 286, 950-951; Lin et al., 1999, Nature, 402, 128-129; Sharp, 1999, Genes & Dev., 13:139-141; and Strauss, 1999, Science, 286, 886). The presence of dsRNA in cells triggers the RNAi response through a mechanism that has yet to be fully characterized.

Dicer is involved in the processing of the dsRNA into short pieces of dsRNA known as short interfering RNAs (siRNAs) (Zamore et al., 2000, Cell, 101, 25-33; Bass, 2000, Cell, 101, 235; Berstein et aL, 2001, Nature, 409, 363). siRNAs derived from dicer activity can be about 21 to about 23 nucleotides in length and include about 19 base pair duplexes (Zamore et al., 2000, Cell, 101, 25-33; Elbashir et aL, 2001, Genes Dev., 15, 188). Dicer has also been implicated in the excision of 21- and 22-nucleotide small temporal RNAs (stRNAs) from precursor RNA of conserved structure that are implicated in translational control (Hutvagner et al., 2001, Science, 293, 834). The RNAi response also features an endonuclease complex, commonly referred to as an RNA-induced silencing complex (RISC), which mediates cleavage of single-stranded RNA having sequence complementary to the antisense strand of the siRNA duplex. Cleavage of the target RNA takes place in the middle of the region complementary to the antisense strand of the siRNA duplex (Elbashir et al., 2001, Genes Dev., 15, 188).

RNAi has been studied in a variety of systems. Fire et al., 1998, Nature, 391, 806, were the first to observe RNAi in C. elegans. Bahramian and Zarbl, 1999, Molecular and Cellular Biology, 19, 274-283 and Wianny and Goetz, 1999, Nature Cell Biol., 2, 70, describe RNAi mediated by dsRNA in mammalian systems. Elbashir et al., 2001, Nature, 411, 494 and Tuschl et al., WO0175164, describe RNAi induced by introduction of duplexes of synthetic 21-nucleotide RNAs in cultured mammalian cells including human embryonic kidney and HeLa cells. Recent work (Elbashir et al., 2001, EMBO J., 20, 6877 and Tuschl et al., WO0175164) has revealed certain requirements for siRNA length, structure, chemical composition, and sequence that are essential to mediate efficient RNAi activity.

Nucleic acid molecules (for example comprising structural features as disclosed herein) may inhibit or down regulate gene expression or viral replication by mediating RNA interference “RNAi” or gene silencing in a sequence-specific manner. (See, e.g., Zamore et al., 2000, Cell, 101, 25-33; Bass, 2001, Nature, 411, 428-429; Elbashir et al., 2001, Nature, 411, 494-498; and Kreutzer et al., WO0044895; Zernicka-Goetz et al., WO0136646; Fire, WO9932619; Plaetinck et al., WO0001846; Mello and Fire, WO0129058; Deschamps-Depaillette, WO9907409; and Li et al., WO0044914; Allshire, 2002, Science, 297, 1818-1819; Volpe et al., 2002, Science, 297, 1833-1837; Jenuwein, 2002, Science, 297, 2215-2218; and Hall et al., 2002, Science, 297, 2232-2237; Hutvagner and Zamore, 2002, Science, 297, 2056-60; McManus et al., 2002, RNA, 8, 842-850; Reinhart et al., 2002, Gene & Dev., 16, 1616-1626; and Reinhart & Bartel, 2002, Science, 297, 1831)

An siNA nucleic acid molecule can be assembled from two separate polynucleotide strands, where one strand is the sense strand and the other is the antisense strand in which the antisense and sense strands are self-complementary (i.e. each strand includes nucleotide sequence that is complementary to nucleotide sequence in the other strand); such as where the antisense strand and sense strand form a duplex or double-stranded structure having any length and structure as described herein for nucleic acid molecules as provided, for example wherein the double-stranded region (duplex region) is about 15 to about 49 (e.g., about 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, or 49 base pairs); the antisense strand includes nucleotide sequence that is complementary to nucleotide sequence in a target nucleic acid molecule (i.e., hsp47 mRNA) or a portion thereof and the sense strand includes nucleotide sequence corresponding to the target nucleic acid sequence or a portion thereof (e.g., about 17 to about 49 or more nucleotides of the nucleic acid molecules herein are complementary to the target nucleic acid or a portion thereof).

In certain aspects and embodiments, a nucleic acid molecule (e.g., a siNA molecule) provided herein may be a “RISC length” molecule or may be a Dicer substrate as described in more detail below.

An siNA nucleic acid molecule may include separate sense and antisense sequences or regions, where the sense and antisense regions are covalently linked by nucleotide or non-nucleotide linkers molecules as is known in the art, or are alternately non-covalently linked by ionic interactions, hydrogen bonding, van der Waals interactions, hydrophobic interactions, and/or stacking interactions. Nucleic acid molecules may include a nucleotide sequence that is complementary to nucleotide sequence of a target gene. Nucleic acid molecules may interact with nucleotide sequence of a target gene in a manner that causes inhibition of expression of the target gene.

Alternatively, an siNA nucleic acid molecule is assembled from a single polynucleotide, where the self-complementary sense and antisense regions of the nucleic acid molecules are linked by means of a nucleic acid based or non-nucleic acid-based linker(s), i.e., the antisense strand and the sense strand are part of one single polynucleotide that having an antisense region and sense region that fold to form a duplex region (for example to form a “hairpin” structure as is well known in the art). Such siNA nucleic acid molecules can be a polynucleotide with a duplex, asymmetric duplex, hairpin or asymmetric hairpin secondary structure, having self-complementary sense and antisense regions, wherein the antisense region includes nucleotide sequence that is complementary to nucleotide sequence in a separate target nucleic acid molecule or a portion thereof and the sense region having nucleotide sequence corresponding to the target nucleic acid sequence (e.g., a sequence of hsp47 mRNA). Such siNA nucleic acid molecules can be a circular single-stranded polynucleotide having two or more loop structures and a stem comprising self-complementary sense and antisense regions, wherein the antisense region includes nucleotide sequence that is complementary to nucleotide sequence in a target nucleic acid molecule or a portion thereof and the sense region having nucleotide sequence corresponding to the target nucleic acid sequence or a portion thereof, and wherein the circular polynucleotide can be processed either in vivo or in vitro to generate an active nucleic acid molecule capable of mediating RNAi.

The following nomenclature is often used in the art to describe lengths and overhangs of siNA molecules and may be used throughout the specification and Examples. In all descriptions of oligonucleotides herein, the identification of nucleotides in a sequence is given in the 5′ to 3′ direction for both sense and antisense strands. Names given to duplexes indicate the length of the oligomers and the presence or absence of overhangs. For example, a “21+2” duplex contains two nucleic acid strands both of which are 21 nucleotides in length, also termed a 21-mer siRNA duplex or a 21-mer nucleic acid and having a 2 nucleotides 3′-overhang. A “21−2” design refers to a 21-mer nucleic acid duplex with a 2 nucleotides 5′-overhang. A 21−0 design is a 21-mer nucleic acid duplex with no overhangs (blunt). A “21+2UU” is a 21-mer duplex with 2-nucleotides 3′-overhang and the terminal 2 nucleotides at the 3′-ends are both U residues (which may result in mismatch with target sequence). The aforementioned nomenclature can be applied to siNA molecules of various lengths of strands, duplexes and overhangs (such as 19−0, 21+2, 27+2, and the like). In an alternative but similar nomenclature, a “25/27” is an asymmetric duplex having a 25 base sense strand and a 27 base antisense strand with a 2-nucleotides 3′-overhang. A “27/25” is an asymmetric duplex having a 27 base sense strand and a 25 base antisense strand.

Chemical Modifications

In certain aspects and embodiments, nucleic acid molecules (e.g., siNA molecules) as provided herein include one or more modifications (or chemical modifications). In certain embodiments, such modifications include any changes to a nucleic acid molecule or polynucleotide that would make the molecule different than a standard ribonucleotide or RNA molecule (i.e., that includes standard adenosine, cytosine, uracil, or guanosine moieties); which may be referred to as an “unmodified” ribonucleotide or unmodified ribonucleic acid. Traditional DNA bases and polynucleotides having a 2′-deoxy sugar represented by adenosine, cytosine, thymine, or guanosine moieties may be referred to as an “unmodified deoxyribonucleotide” or “unmodified deoxyribonucleic acid”; accordingly, the term “unmodified nucleotide” or “unmodified nucleic acid” as used herein refers to an “unmodified ribonucleotide” or “unmodified ribonucleic acid” unless there is a clear indication to the contrary. Such modifications can be in the nucleotide sugar, nucleotide base, nucleotide phosphate group and/or the phosphate backbone of a polynucleotide.

In certain embodiments, modifications as disclosed herein may be used to increase RNAi activity of a molecule and/or to increase the in vivo stability of the molecules, particularly the stability in serum, and/or to increase bioavailability of the molecules. Non-limiting examples of modifications include internucleotide or internucleoside linkages; deoxynucleotides or dideoxyribonucleotides at any position and strand of the nucleic acid molecule; nucleic acid (e.g., ribonucleic acid) with a modification at the 2′-position preferably selected from an amino, fluoro, methoxy, alkoxy and alkyl; 2′-deoxyribonucleotides, 2′OMe ribonucleotides, 2′-deoxy-2′-fluoro ribonucleotides, “universal base” nucleotides, “acyclic” nucleotides, 5-C-methyl nucleotides, biotin group, and terminal glyceryl and/or inverted deoxy abasic residue incorporation, sterically hindered molecules, such as fluorescent molecules and the like. Other nucleotides modifiers could include 3′-deoxyadenosine (cordycepin), 3′-azido-3′-deoxythymidine (AZT), 2′,3′-dideoxyinosine (ddI), 2′,3′-dideoxy-3′-thiacytidine (3TC), 2′,3′-didehydro-2′,3′-dideoxythymidine (d4T) and the monophosphate nucleotides of 3′-azido-3′-deoxythymidine (AZT), 2′,3′-dideoxy-3′-thiacytidine (3TC) and 2′,3′-didehydro-2′,3′-dide-oxythymidine (d4T). Further details on various modifications are described in more detail below.

Modified nucleotides include those having a Northern conformation (e.g., Northern pseudorotation cycle, see for example Sanger, Principles of Nucleic Acid Structure, Springer-Verlag ed., 1984). Non-limiting examples of nucleotides having a northern configuration include LNA nucleotides (e.g., 2′-O, 4′-C-methylene-(D-ribofuranosyl) nucleotides); 2′-methoxyethoxy (MOE) nucleotides; 2′-methyl-thio-ethyl, 2′-deoxy-2′-fluoro nucleotides, 2′-deoxy-2′-chloro nucleotides, 2′-azido nucleotides, and 2′OMe nucleotides. LNAs are described, for example, in Elman et al., 2005; Kurreck et al., 2002; Crinelli et al., 2002; Braasch and Corey, 2001; Bondensgaard et al., 2000; Wahlestedt et al., 2000; and WO0047599, WO9914226, WO9839352, and WO04083430. In one embodiment, an LNA is incorporated at the 5′-terminus of the sense strand.

Chemical modifications also include DNAs, which are non-nucleotide, acyclic analogues, in which the C2′-C3′ bond is not present (although DNAs are not truly nucleotides, they are expressly included in the scope of “modified” nucleotides or modified nucleic acids as contemplated herein). In particular embodiments, nucleic acid molecules with an overhang may be modified to have DNAs at the overhang positions (i.e., 2 nucleotide overhand). In other embodiments, DNAs are included at the 3′- or 5′-ends. A UNA may be located anywhere along a nucleic acid strand, i.e. in position 7. Nucleic acid molecules may contain one or more than UNA. Exemplary DNAs are disclosed in Nucleic Acids Symposium Series No. 52 p. 133-134 (2008). In certain embodiments, nucleic acid molecules (e.g., siNA molecules) as described herein, include one or more DNAs; or one UNA. In some embodiments, a nucleic acid molecule (e.g., a siNA molecule) as described herein that has a 3′-overhang include one or two DNAs in the 3′ overhang. In some embodiments, a nucleic acid molecule (e.g., a siNA molecule) as described herein includes a UNA (for example one UNA) in the antisense strand; for example in position 6 or position 7 of the antisense strand. Chemical modifications also include non-pairing nucleotide analogs, for example as disclosed herein. Chemical modifications further include unconventional moieties as disclosed herein.

Chemical modifications also include terminal modifications on the 5′ and/or 3′ part of the oligonucleotides and are also known as capping moieties. Such terminal modifications are selected from a nucleotide, a modified nucleotide, a lipid, a peptide, and a sugar.

Chemical modifications also include six membered “six membered ring nucleotide analogs.” Examples of six-membered ring nucleotide analogs are disclosed in Allart, et al (Nucleosides & Nucleotides, 1998, 17:1523-1526; and Perez-Perez, et al., 1996, Bioorg. and Medicinal Chem Letters 6:1457-1460) Oligonucleotides including 6-membered ring nucleotide analogs including hexitol and altritol nucleotide monomers are disclosed in WO2006047842.

Chemical modifications also include “mirror” nucleotides which have a reversed chirality as compared to normal naturally occurring nucleotide; that is, a mirror nucleotide may be an “L-nucleotide” analogue of naturally occurring D-nucleotide (see U.S. Pat. No. 6,602,858). Mirror nucleotides may further include at least one sugar or base modification and/or a backbone modification, for example, as described herein, such as a phosphorothioate or phosphonate moiety. U.S. Pat. No. 6,602,858 discloses nucleic acid catalysts including at least one L-nucleotide substitution. Mirror nucleotides include for example L-DNA (L-deoxyriboadenosine-3′-phosphate (mirror dA); L-deoxyribocytidine-3′-phosphate (mirror dC); L-deoxyriboguanosine-3′-phosphate (mirror dG); L-deoxyribothymidine-3′-phosphate (mirror image dT)) and L-RNA (L-riboadenosine-3′-phosphate (mirror rA); L-ribocytidine-3′-phosphate (mirror rC); L-riboguanosine-3′-phosphate (mirror rG); L-ribouracil-3′-phosphate (mirror dU).

In some embodiments, modified ribonucleotides include modified deoxyribonucleotides, for example 5′ OMe DNA (5-methyl-deoxyriboguanosine-3′-phosphate) which may be useful as a nucleotide in the 5′ terminal position (position number 1); PACE (deoxyriboadenosine 3′ phosphonoacetate, deoxyribocytidine 3′ phosphonoacetate, deoxyriboguanosine 3′ phosphonoacetate, deoxyribothymidine 3′ phosphonoacetate).

Modifications may be present in one or more strands of a nucleic acid molecule disclosed herein, e.g., in the sense strand, the antisense strand, or both strands. In certain embodiments, the antisense strand may include modifications and the sense strand my only include unmodified RNA.

Nucleobases

Nucleobases of the nucleic acid disclosed herein may include unmodified ribonucleotides (purines and pyrimidines) such as adenine, guanine, cytosine, uracil. The nucleobases in one or both strands can be modified with natural and synthetic nucleobases such as, thymine, xanthine, hypoxanthine, inosine, 2-aminoadenine, 6-methyl and other alkyl derivatives of adenine and guanine, any “universal base” nucleotides; 2-propyl and other alkyl derivatives of adenine and guanine, 5-halo uracil and cytosine, 5-propynyl uracil and cytosine, 6-azo uracil, cytosine and thymine, 5-uracil (pseudo-uracil), 4-thiouracil, 8-halo, amino, thiol, thioalkyl, hydroxyl and other 8-substituted adenines and guanines, 5-trifluoromethyl and other 5-substituted uracils and cytosines, 7-methylguanine, deazapurines, heterocyclic substituted analogs of purines and pyrimidines, e.g., aminoethyoxy phenoxazine, derivatives of purines and pyrimidines (e.g., 1-alkyl-, 1-alkenyl-, heteroaromatic- and 1-alkynyl derivatives) and tautomers thereof, 8-oxo-N6-methyladenine, 7-diazaxanthine, 5-methylcytosine, 5-methyluracil, 5-(1-propynyl)uracil, 5-(1-propynyl) cytosine and 4,4-ethanocytosine). Other examples of suitable bases include non-purinyl and non-pyrimidinyl bases such as 2-aminopyridine and triazines.

Sugar Moieties

Sugar moieties in a nucleic acid disclosed herein may include 2′-hydroxyl-pentofuranosyl sugar moiety without any modification. Alternatively, sugar moieties can be modified such as, 2′-deoxy-pentofuranosyl sugar moiety, D-ribose, hexose, modification at the 2′ position of the pentofuranosyl sugar moiety such as 2′-O-alkyl (including 2′OMe and 2′-O-ethyl), i.e., 2′-alkoxy, 2′-amino, 2′-O-allyl, 2′-S-alkyl, 2′-halogen (including 2′-fluoro, chloro, and bromo), 2′-methoxyethoxy, 2′-O-methoxyethyl, 2′-O-2-methoxyethyl, 2′-allyloxy (—OCH₂CH═CH₂), 2′-propargyl, 2′-propyl, ethynyl, ethenyl, propenyl, CF, cyano, imidazole, carboxylate, thioate, C₁ to C₁₀ lower alkyl, substituted lower alkyl, alkaryl or aralkyl, OCF₃, OCN, O-, S-, or N-alkyl; 0-, S, or N-alkenyl; SOCH₃; SO₂CH₃; ONO₂; NO₂, N₃; heterozycloalkyl; heterozycloalkaryl; aminoalkylamino; polyalkylamino or substituted silyl, as, among others, for example as described in European patents EP0586520 or EP0618925.

Alkyl group includes saturated aliphatic groups, including straight-chain alkyl groups (e.g., methyl, ethyl, propyl, butyl, pentyl, hexyl, heptyl, octyl, nonyl, decyl, etc.), branched-chain alkyl groups (isopropyl, tert-butyl, isobutyl, etc.), cycloalkyl (alicyclic) groups (cyclopropyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl), alkyl substituted cycloalkyl groups, and cycloalkyl substituted alkyl groups. In certain embodiments, a straight chain or branched chain alkyl has six or fewer carbon atoms in its backbone (e.g., C₁-C₆ for straight chain, C₃-C₆ for branched chain), and more preferably four or fewer. Likewise, preferred cycloalkyls may have from three to eight carbon atoms in their ring structure, and more preferably have five or six carbons in the ring structure. The term C₁-C₆ includes alkyl groups containing one to six carbon atoms. The alkyl group can be substituted alkyl group such as alkyl moieties having substituents replacing a hydrogen on one or more carbons of the hydrocarbon backbone. Such substituents can include, for example, alkenyl, alkynyl, halogen, hydroxyl, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, arylcarbonyl, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl, alkylthiocarbonyl, alkoxyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido), amidino, imino, sulfhydryl, alkylthio, arylthio, thiocarboxylate, sulfates, alkylsulfinyl, sulfonato, sulfamoyl, sulfonamido, nitro, trifluoromethyl, cyano, azido, heterocyclyl, alkylaryl, or an aromatic or heteroaromatic moiety.

Alkoxy group includes substituted and unsubstituted alkyl, alkenyl, and alkynyl groups covalently linked to an oxygen atom. Examples of alkoxy groups include methoxy, ethoxy, isopropyloxy, propoxy, butoxy, and pentoxy groups. Examples of substituted alkoxy groups include halogenated alkoxy groups. The alkoxy groups can be substituted with groups such as alkenyl, alkynyl, halogen, hydroxyl, alkylcarbonyloxy, arylcarbonyloxy, alkoxycarbonyloxy, aryloxycarbonyloxy, carboxylate, alkylcarbonyl, arylcarbonyl, alkoxycarbonyl, aminocarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl, alkylthiocarbonyl, alkoxyl, phosphate, phosphonato, phosphinato, cyano, amino (including alkyl amino, dialkylamino, arylamino, diarylamino, and alkylarylamino), acylamino (including alkylcarbonylamino, arylcarbonylamino, carbamoyl and ureido), amidino, imino, sulfhydryl, alkylthio, arylthio, thiocarboxylate, sulfates, alkylsulfinyl, sulfonato, sulfamoyl, sulfonamido, nitro, trifluoromethyl, cyano, azido, heterocyclyl, alkylaryl, or an aromatic or heteroaromatic moieties. Examples of halogen substituted alkoxy groups include, but are not limited to, fluoromethoxy, difluoromethoxy, trifluoromethoxy, chloromethoxy, dichloromethoxy, trichloromethoxy, etc.

In some embodiments, the pentafuronosyl ring may be replaced with acyclic derivatives lacking the C₂′-C₃′-bond of the pentafuronosyl ring. For example, acyclonucleotides may substitute a 2-hydroxyethoxymethyl group for—the 2′-deoxyribofuranosyl sugar normally present in dNMPs.

Halogens include fluorine, bromine, chlorine, iodine.

Backbone

The nucleoside subunits of the nucleic acid disclosed herein may be linked to each other by a phosphodiester bond. The phosphodiester bond may be optionally substituted with other linkages. For example, phosphorothioate, thiophosphate-D-ribose entities, triester, thioate, 2′-5′ bridged backbone (may also be referred to as 5′-2′ or 2′-5′-nucleotide or 2′-5′-ribonucleotide), PACE, 3′(or -5′)-deoxy-3′(or -5′)-thio-phosphorothioate, phosphorodithioate, phosphoroselenates, 3′(or -5′)-deoxy phosphinates, borano phosphates, 3′(or -5′-)-deoxy-3′(or 5′-)-amino phosphoramidates, hydrogen phosphonates, phosphonates, borano phosphate esters, phosphoramidates, alkyl or aryl phosphonates and phosphotriester modifications such as alkylphosphotriesters, phosphotriester phosphorus linkages, 5′-ethoxyphosphodiester, P-alkyloxyphosphotriester, methylphosphonate, and nonphosphorus containing linkages for example, carbonate, carbamate, silyl, sulfur, sulfonate, sulfonamide, formacetal, thioformacetyl, oxime, methyleneimino, methylenemethylimino, methylenehydrazo, methylenedimethylhydrazo and methyleneoxymethylimino linkages.

Nucleic acid molecules disclosed herein may include a peptide nucleic acid (PNA) backbone. The PNA backbone is includes repeating N-(2-aminoethyl)-glycine units linked by peptide bonds. The various bases such as purine, pyrimidine, natural and synthetic bases are linked to the backbone by methylene carbonyl bonds.

Terminal Phosphates

Modifications can be made at terminal phosphate groups. Non-limiting examples of different stabilization chemistries can be used, e.g., to stabilize the 3′-end of nucleic acid sequences, including (3′-3′)-inverted deoxyribose; deoxyribonucleotide; (5′-3′)-3′-deoxyribonucleotide; (5′-3′)-ribonucleotide; (5′-3′)-3′-O-methyl ribonucleotide; 3′-glyceryl; (3′-5′)-3′-deoxyribonucleotide; (3′-3′)-deoxyribonucleotide; (5′-2′)-deoxyribonucleotide; and (5-3′)-dideoxyribonucleotide. In addition, unmodified backbone chemistries can be combined with one or more different backbone modifications described herein.

Exemplary chemically modified terminal phosphate groups include those shown below:

Conjugates

Modified nucleotides and nucleic acid molecules (e.g., siNA molecules) as provided herein may include conjugates, for example, a conjugate covalently attached to the chemically-modified nucleic acid molecule. Non-limiting examples of conjugates include conjugates and ligands described in Vargeese et al., U.S. Ser. No. 10/427,160. The conjugate may be covalently attached to a nucleic acid molecule (such as an siNA molecule) via a biodegradable linker. The conjugate molecule may be attached at the 3′-end of either the sense strand, the antisense strand, or both strands of the chemically-modified nucleic acid molecule. The conjugate molecule may be attached at the 5′-end of either the sense strand, the antisense strand, or both strands of the chemically-modified nucleic acid molecule. The conjugate molecule may be attached both the 3′-end and 5′-end of either the sense strand, the antisense strand, or both strands of the chemically-modified nucleic acid molecule, or any combination thereof. In one embodiment, a conjugate molecule may include a molecule that facilitates delivery of a chemically-modified nucleic acid molecule into a biological system, such as a cell. In another embodiment, the conjugate molecule attached to the chemically-modified nucleic acid molecule is a polyethylene glycol, human serum albumin, or a ligand for a cellular receptor that can mediate cellular uptake. Examples of specific conjugate molecules contemplated by the instant description that can be attached to chemically-modified nucleic acid molecules are described in Vargeese et al., U.S. Ser. No. 10/201,394.

Linkers

A nucleic acid molecule provided herein (e.g., an siNA) molecule may include a nucleotide, non-nucleotide, or mixed nucleotide/non-nucleotide linker that joins the sense region of the nucleic acid to the antisense region of the nucleic acid. A nucleotide linker can be a linker of ≧2 nucleotides in length, for example about 3, 4, 5, 6, 7, 8, 9, or 10 nucleotides in length. The nucleotide linker can be a nucleic acid aptamer. By “aptamer” or “nucleic acid aptamer” as used herein refers to a nucleic acid molecule that binds specifically to a target molecule wherein the nucleic acid molecule has sequence that includes a sequence recognized by the target molecule in its natural setting. Alternately, an aptamer can be a nucleic acid molecule that binds to a target molecule (such as hsp47 mRNA) where the target molecule does not naturally bind to a nucleic acid. For example, the aptamer can be used to bind to a ligand-binding domain of a protein, thereby preventing interaction of the naturally occurring ligand with the protein. This is a non-limiting example and those in the art will recognize that other embodiments can be readily generated using techniques generally known in the art. See e.g., Gold et al.; 1995, Annu. Rev. Biochem., 64, 763; Brody and Gold, 2000, J. Biotechnol., 74, 5; Sun, 2000, Curr. Opin. Mol. Ther., 2, 100; Kusser, 2000, J. Biotechnol., 74, 27; Hermann and Patel, 2000, Science, 287, 820; and Jayasena, 1999, Clinical Chemistry, 45, 1628.

A non-nucleotide linker may include an abasic nucleotide, polyether, polyamine, polyamide, peptide, carbohydrate, lipid, polyhydrocarbon, or other polymeric compounds (e.g. polyethylene glycols such as those having between 2 and 100 ethylene glycol units). Specific examples include those described by Seela and Kaiser, Nucleic Acids Res. 1990, 18:6353 and Nucleic Acids Res. 1987, 15:3113; Cload and Schepartz, J. Am. Chem. Soc. 1991, 113:6324; Richardson and Schepartz, J. Am. Chem. Soc. 1991, 113:5109; Ma et al., Nucleic Acids Res. 1993, 21:2585 and Biochemistry 1993, 32:1751; Durand et al., Nucleic Acids Res. 1990, 18:6353; McCurdy et al., Nucleosides & Nucleotides 1991, 10:287; Jschke et al., Tetrahedron Lett. 1993, 34:301; Ono et al., Biochemistry 1991, 30:9914; Arnold et al., WO8902439; Usman et al., WO9506731; Dudycz et al., WO9511910 and Ferentz and Verdine, J. Am. Chem. Soc. 1991, 113:4000.

5′-Ends, 3′-Ends, and Overhangs

Nucleic acid molecules disclosed herein (e.g., siNA molecules) may be blunt-ended on both sides, have overhangs on both sides, or a combination of blunt and overhang ends. Overhangs may occur on either the 5′- or 3′-end of the sense or antisense strand.

5′- and/or 3′-ends of double-stranded nucleic acid molecules (e.g., siNA) may be blunt ended or have an overhang. The 5′-end may be blunt ended and the 3′-end has an overhang in either the sense strand or the antisense strand. In other embodiments, the 3′-end may be blunt ended and the 5′-end has an overhang in either the sense strand or the antisense strand. In yet other embodiments, both the 5′- and 3′-end are blunt ended or both the 5′- and 3′-ends have overhangs.

The 5′- and/or 3′-end of one or both strands of the nucleic acid may include a free hydroxyl group. The 5′- and/or 3′-end of any nucleic acid molecule strand may be modified to include a chemical modification. Such modification may stabilize nucleic acid molecules, e.g., the 3′-end may have increased stability due to the presence of the nucleic acid molecule modification. Examples of end modifications (e.g., terminal caps) include, but are not limited to, abasic, deoxy abasic, inverted (deoxy) abasic, glyceryl, dinucleotide, acyclic nucleotide, amino, fluoro, chloro, bromo, CN, CF, methoxy, imidazole, carboxylate, thioate, C₁ to C₁₀ lower alkyl, substituted lower alkyl, alkaryl or aralkyl, OCF₃, OCN, O-, S-, or N-alkyl; O-, S-, or N-alkenyl; SOCH₃; SO₂CH₃; ONO₂; NO₂, N₃; heterocycloalkyl; heterocycloalkaryl; aminoalkylamino; polyalkylamino or substituted silyl, as, among others, described in European patents EP586520 and EP618925 and other modifications disclosed herein.

Nucleic acid molecules include those with blunt ends, i.e., ends that do not include any overhanging nucleotides. A nucleic acid molecule can include one or more blunt ends. The blunt ended nucleic acid molecule has a number of base pairs equal to the number of nucleotides present in each strand of the nucleic acid molecule. The nucleic acid molecule can include one blunt end, for example where the 5′-end of the antisense strand and the 3′-end of the sense strand do not have any overhanging nucleotides. Nucleic acid molecule may include one blunt end, for example where the 3′-end of the antisense strand and the 5′-end of the sense strand do not have any overhanging nucleotides. A nucleic acid molecule may include two blunt ends, for example where the 3′-end of the antisense strand and the 5′-end of the sense strand as well as the 5′-end of the antisense strand and 3′-end of the sense strand do not have any overhanging nucleotides. Other nucleotides present in a blunt ended nucleic acid molecule can include, for example, mismatches, bulges, loops, or wobble base pairs to modulate the activity of the nucleic acid molecule to mediate RNA interference.

In certain embodiments of the nucleic acid molecules (e.g., siNA molecules) provided herein, at least one end of the molecule has an overhang of at least one nucleotide (for example one to eight overhang nucleotides). For example, one or both strands of a double-stranded nucleic acid molecule disclosed herein may have an overhang at the 5′-end or at the 3′-end or both. An overhang may be present at either or both the sense strand and antisense strand of the nucleic acid molecule. The length of the overhang may be as little as one nucleotide and as long as one to eight or more nucleotides (e.g., 1, 2, 3, 4, 5, 6, 7 or 8 nucleotides; in some preferred embodiments an overhang is 2, 3, 4, 5, 6, 7 or 8 nucleotides; for example an overhang may be 2 nucleotides. The nucleotide(s) forming the overhang may be include deoxyribonucleotide(s), ribonucleotide(s), natural and non-natural nucleobases or any nucleotide modified in the sugar, base or phosphate group such as disclosed herein. A double-stranded nucleic acid molecule may have both 5′- and 3′-overhangs. The overhangs at the 5′- and 3′-end may be of different lengths. An overhang may include at least one nucleic acid modification which may be deoxyribonucleotide. One or more deoxyribonucleotides may be at the 5′-terminal. The 3′-end of the respective counter-strand of the nucleic acid molecule may not have an overhang, more preferably not a deoxyribonucleotide overhang. The one or more deoxyribonucleotide may be at the 3′-terminal. The 5′-end of the respective counter-strand of the dsRNA may not have an overhang, more preferably not a deoxyribonucleotide overhang. The overhang in either the 5′- or the 3′-end of a strand may be one to eight (e.g., about 1, 2, 3, 4, 5, 6, 7 or 8) unpaired nucleotides, preferably, the overhang is two to three unpaired nucleotides; more preferably two unpaired nucleotides. Nucleic acid molecules may include duplex nucleic acid molecules with overhanging ends of about 1 to about 20 (e.g., about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 1, 15, 16, 17, 18, 19 or 20); preferably one to eight (e.g., about 1, 2, 3, 4, 5, 6, 7 or 8) nucleotides, for example, about 21-nucleotide duplexes with about 19 base pairs and 3′-terminal mononucleotide, dinucleotide, or trinucleotide overhangs. Nucleic acid molecules herein may include duplex nucleic acid molecules with blunt ends, where both ends are blunt, or alternatively, where one of the ends is blunt. Nucleic acid molecules disclosed herein can include one or more blunt ends, i.e. where a blunt end does not have any overhanging nucleotides. In one embodiment, the blunt ended nucleic acid molecule has a number of base pairs equal to the number of nucleotides present in each strand of the nucleic acid molecule. The nucleic acid molecule may include one blunt end, for example where the 5′-end of the antisense strand and the 3′-end of the sense strand do not have any overhanging nucleotides. The nucleic acid molecule may include one blunt end, for example where the 3′-end of the antisense strand and the 5′-end of the sense strand do not have any overhanging nucleotides. A nucleic acid molecule may include two blunt ends, for example where the 3′-end of the antisense strand and the 5′-end of the sense strand as well as the 5′-end of the antisense strand and 3′-end of the sense strand do not have any overhanging nucleotides. In certain preferred embodiments the nucleic acid compounds are blunt ended. Other nucleotides present in a blunt ended siNA molecule can include, for example, mismatches, bulges, loops, or wobble base pairs to modulate the activity of the nucleic acid molecule to mediate RNA interference.

In many embodiments one or more, or all, of the overhang nucleotides of a nucleic acid molecule (e.g., a siNA molecule) as described herein includes are modified such as described herein; for example one or more, or all, of the nucleotides may be 2′-deoxynucleotides.

Amount, Location and Patterns of Modifications

Nucleic acid molecules (e.g., siNA molecules) disclosed herein may include modified nucleotides as a percentage of the total number of nucleotides present in the nucleic acid molecule. As such, a nucleic acid molecule may include about 5% to about 100% modified nucleotides (e.g., about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% modified nucleotides). The actual percentage of modified nucleotides present in a given nucleic acid molecule will depend on the total number of nucleotides present in the nucleic acid. If the nucleic acid molecule is single-stranded, the percent modification can be based upon the total number of nucleotides present in the single-stranded nucleic acid molecule. Likewise, if the nucleic acid molecule is double-stranded, the percent modification can be based upon the total number of nucleotides present in the sense strand, antisense strand, or both the sense and antisense strands.

Nucleic acid molecules disclosed herein may include unmodified RNA as a percentage of the total nucleotides in the nucleic acid molecule. As such, a nucleic acid molecule may include about 5% to about 100% modified nucleotides (e.g., about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 100% of total nucleotides present in a nucleic acid molecule.

A nucleic acid molecule (e.g., an siNA molecule) may include a sense strand that includes about one to about five, specifically about 1, 2, 3, 4, or 5 phosphorothioate internucleotide linkages, and/or one or more (e.g., about 1, 2, 3, 4, 5, or more) 2′-deoxy, 2′OMe, 2′-deoxy-2′-fluoro, and/or one or more (e.g., about 1, 2, 3, 4, 5, or more) universal base modified nucleotides, and optionally a terminal cap molecule at the 3-end, the 5′-end, or both of the 3′- and 5′-ends of the sense strand; and wherein the antisense strand includes about one to about five or more, specifically about 1, 2, 3, 4, 5, or more phosphorothioate internucleotide linkages, and/or one or more (e.g., about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more) 2′-deoxy, 2′OMe, 2′-deoxy-2′-fluoro, and/or one or more (e.g., about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more) universal base modified nucleotides, and optionally a terminal cap molecule at the 3′-end, the 5′-end, or both of the 3′- and 5′-ends of the antisense strand. A nucleic acid molecule may include about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more, pyrimidine nucleotides of the sense and/or antisense nucleic acid strand are chemically-modified with 2′-deoxy, 2′OMe and/or 2′-deoxy-2′-fluoro nucleotides, with or without about one to about five or more, for example about 1, 2, 3, 4, 5, or more phosphorothioate internucleotide linkages and/or a terminal cap molecule at the 3′-end, the 5′-end, or both of the 3′- and 5′-ends, being present in the same or different strand.

A nucleic acid molecule may include about one to about five or more (specifically about 1, 2, 3, 4, 5, or more) phosphorothioate internucleotide linkages in each strand of the nucleic acid molecule.

A nucleic acid molecule may include 2′-5′ internucleotide linkages, for example at the 3′-end, the 5′-end, or both of the 3′- and 5′-ends of one or both nucleic acid sequence strands. In addition, the 2′-5′ internucleotide linkage(s) can be present at various other positions within one or both nucleic acid sequence strands, for example, about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more including every internucleotide linkage of a pyrimidine nucleotide in one or both strands of the siNA molecule can include a 2′-5′ internucleotide linkage, or about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or more including every internucleotide linkage of a purine nucleotide in one or both strands of the siNA molecule can include a 2′-5′ internucleotide linkage.

A chemically-modified short interfering nucleic acid (siNA) molecule may include an antisense region, wherein any (e.g., one or more or all) pyrimidine nucleotides present in the antisense region are 2′-deoxy-2′-fluoro pyrimidine nucleotides (e.g., wherein all pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides or alternately a plurality of pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides), and wherein any (e.g., one or more or all) purine nucleotides present in the antisense region are 2′-deoxy purine nucleotides (e.g., wherein all purine nucleotides are 2′-deoxy purine nucleotides or alternately a plurality of purine nucleotides are 2′-deoxy purine nucleotides).

A chemically-modified short interfering nucleic acid (siNA) molecule may include an antisense region, wherein any (e.g., one or more or all) pyrimidine nucleotides present in the antisense region are 2′-deoxy-2′-fluoro pyrimidine nucleotides (e.g., wherein all pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides or alternately a plurality of pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides), and wherein any (e.g., one or more or all) purine nucleotides present in the antisense region are 2′OMe purine nucleotides (e.g., wherein all purine nucleotides are 2′OMe purine nucleotides or alternately a plurality of purine nucleotides are 2′OMe purine nucleotides).

A chemically-modified siNA molecule capable of mediating RNA interference (RNAi) against hsp47 inside a cell or reconstituted in vitro system may include a sense region, wherein one or more pyrimidine nucleotides present in the sense region are 2′-deoxy-2′-fluoro pyrimidine nucleotides (e.g., wherein all pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides or alternately a plurality of pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides), and one or more purine nucleotides present in the sense region are 2′-deoxy purine nucleotides (e.g., wherein all purine nucleotides are 2′-deoxy purine nucleotides or alternately a plurality of purine nucleotides are 2′-deoxy purine nucleotides), and an antisense region, wherein one or more pyrimidine nucleotides present in the antisense region are 2′-deoxy-2′-fluoro pyrimidine nucleotides (e.g., wherein all pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides or alternately a plurality of pyrimidine nucleotides are 2′-deoxy-2′-fluoro pyrimidine nucleotides), and one or more purine nucleotides present in the antisense region are 2′OMe purine nucleotides (e.g., wherein all purine nucleotides are 2′OMe purine nucleotides or alternately a plurality of purine nucleotides are 2′OMe purine nucleotides). The sense region and/or the antisense region can have a terminal cap modification, such as any modification that is optionally present at the 3′-end, the 5′-end, or both of the 3′ and 5′-ends of the sense and/or antisense sequence. The sense and/or antisense region can optionally further include a 3′-terminal nucleotide overhang having about 1 to about 4 (e.g., about 1, 2, 3, or 4) 2′-deoxynucleotides. The overhang nucleotides can further include one or more (e.g., about 1, 2, 3, 4 or more) phosphorothioate, phosphonoacetate, and/or thiophosphonoacetate internucleotide linkages. The purine nucleotides in the sense region may alternatively be 2′OMe purine nucleotides (e.g., wherein all purine nucleotides are 2′OMe purine nucleotides or alternately a plurality of purine nucleotides are 2′OMe purine nucleotides) and one or more purine nucleotides present in the antisense region are 2′OMe purine nucleotides (e.g., wherein all purine nucleotides are 2′OMe purine nucleotides or alternately a plurality of purine nucleotides are 2′OMe purine nucleotides). One or more purine nucleotides in the sense region may alternatively be purine ribonucleotides (e.g., wherein all purine nucleotides are purine ribonucleotides or alternately a plurality of purine nucleotides are purine ribonucleotides) and any purine nucleotides present in the antisense region are 2′OMe purine nucleotides (e.g., wherein all purine nucleotides are 2′OMe purine nucleotides or alternately a plurality of purine nucleotides are 2′OMe purine nucleotides). One or more purine nucleotides in the sense region and/or present in the antisense region may alternatively selected from the group consisting of 2′-deoxy nucleotides, LNA nucleotides, 2′-methoxyethyl nucleotides, 4′-thionucleotides, and 2′OMe nucleotides (e.g., wherein all purine nucleotides are selected from the group consisting of 2′-deoxy nucleotides, LNA nucleotides, 2′-methoxyethyl nucleotides, 4′-thionucleotides, and 2′OMe nucleotides or alternately a plurality of purine nucleotides are selected from the group consisting of 2′-deoxy nucleotides, LNA nucleotides, 2′-methoxyethyl nucleotides, 4′-thionucleotides, and 2′OMe nucleotides).

In some embodiments, a nucleic acid molecule (e.g., a siNA molecule) as described herein includes a modified nucleotide (for example one modified nucleotide) in the antisense strand; for example in position 6 or position 7 of the antisense strand.

Modification Patterns and Alternating Modifications

Nucleic acid molecules (e.g., siNA molecules) provided herein may have patterns of modified and unmodified nucleic acids. A pattern of modification of the nucleotides in a contiguous stretch of nucleotides may be a modification contained within a single nucleotide or group of nucleotides that are covalently linked to each other via standard phosphodiester bonds or, at least partially, through phosphorothioate bonds. Accordingly, a “pattern” as contemplated herein, does not necessarily need to involve repeating units, although it may. Examples of modification patterns that may be used in conjunction with the nucleic acid molecules (e.g., siNA molecules) provided herein include those disclosed in Giese, U.S. Pat. No. 7,452,987. For example, nucleic acid molecules (e.g., siNA molecules) provided herein include those with modification patters such as, similar to, or the same as, the patterns shown diagrammatically in FIG. 2 of U.S. Pat. No. 7,452,987.

A modified nucleotide or group of modified nucleotides may be at the 5′-end or 3′-end of the sense or antisense strand, a flanking nucleotide or group of nucleotides is arrayed on both sides of the modified nucleotide or group, where the flanking nucleotide or group either is unmodified or does not have the same modification of the preceding nucleotide or group of nucleotides. The flanking nucleotide or group of nucleotides may, however, have a different modification. This sequence of modified nucleotide or group of modified nucleotides, respectively, and unmodified or differently modified nucleotide or group of unmodified or differently modified nucleotides may be repeated one or more times.

In some patterns, the 5′-terminal nucleotide of a strand is a modified nucleotide while in other patterns the 5′-terminal nucleotide of a strand is an unmodified nucleotide. In some patterns, the 5′-end of a strand starts with a group of modified nucleotides while in other patterns, the 5′-terminal end is an unmodified group of nucleotides. This pattern may be either on the first stretch or the second stretch of the nucleic acid molecule or on both.

Modified nucleotides of one strand of the nucleic acid molecule may be complementary in position to the modified or unmodified nucleotides or groups of nucleotides of the other strand.

There may be a phase shift between modifications or patterns of modifications on one strand relative to the pattern of modification of the other strand such that the modification groups do not overlap. In one instance, the shift is such that the modified group of nucleotides of the sense strand corresponds to the unmodified group of nucleotides of the antisense strand and vice versa.

There may be a partial shift of the pattern of modification such that the modified groups overlap. The groups of modified nucleotides in any given strand may optionally be the same length, but may be of different lengths. Similarly, groups of unmodified nucleotides in any given strand may optionally be the same length, or of different lengths.

In some patterns, the second (penultimate) nucleotide at the terminus of the strand, is an unmodified nucleotide or the beginning of group of unmodified nucleotides. Preferably, this unmodified nucleotide or unmodified group of nucleotides is located at the 5′-end of the either or both the sense and antisense strands and even more preferably at the terminus of the sense strand. An unmodified nucleotide or unmodified group of nucleotide may be located at the 5′-end of the sense strand. In a preferred embodiment the pattern consists of alternating single modified and unmodified nucleotides.

In some double-stranded nucleic acid molecules include a 2′OMe modified nucleotide and a non-modified nucleotide, preferably a nucleotide which is not 2′OMe modified, are incorporated on both strands in an alternating fashion, resulting in a pattern of alternating 2′OMe modified nucleotides and nucleotides that are either unmodified or at least do not include a 2′OMe modification. In certain embodiments, the same sequence of 2′OMe modification and non-modification exists on the second strand; in other embodiments the alternating 2′OMe modified nucleotides are only present in the sense strand and are not present in the antisense strand; and in yet other embodiments the alternating 2′OMe modified nucleotides are only present in the sense strand and are not present in the antisense strand. In certain embodiments, there is a phase shift between the two strands such that the 2′OMe modified nucleotide on the first strand base pairs with a non-modified nucleotide(s) on the second strand and vice versa. This particular arrangement, i.e. base pairing of 2′OMe modified and non-modified nucleotide(s) on both strands is particularly preferred in certain embodiments. In certain embodiments, the pattern of alternating 2′OMe modified nucleotides exists throughout the entire nucleic acid molecule; or the entire duplex region. In other embodiments the pattern of alternating 2′OMe modified nucleotides exists only in a portion of the nucleic acid; or the entire duplex region.

In “phase shift” patterns, it may be preferred if the antisense strand starts with a 2′OMe modified nucleotide at the 5′-end whereby consequently the second nucleotide is non-modified, the third, fifth, seventh and so on nucleotides are thus again 2′OMe modified whereas the second, fourth, sixth, eighth and the like nucleotides are non-modified nucleotides.

Modification Locations and Patterns

While exemplary patterns are provided in more detail below, all permutations of patterns with of all possible characteristics of the nucleic acid molecules disclosed herein and those known in the art are contemplated (e.g., characteristics include, but are not limited to, length of sense strand, length of antisense strand, length of duplex region, length of hangover, whether one or both ends of a double-stranded nucleic acid molecule is blunt or has an overhang, location of modified nucleic acid, number of modified nucleic acids, types of modifications, whether a double overhang nucleic acid molecule has the same or different number of nucleotides on the overhang of each side, whether a one or more than one type of modification is used in a nucleic acid molecule, and number of contiguous modified/unmodified nucleotides). With respect to all detailed examples provided below, while the duplex region is shown to be 19 nucleotides, the nucleic acid molecules provided herein can have a duplex region ranging from 1 to 49 nucleotides in length as each strand of a duplex region can independently be 17-49 nucleotides in length Exemplary patterns are provided herein.

Nucleic acid molecules may have a blunt end (when n is 0) on both ends that include a single or contiguous set of modified nucleic acids. The modified nucleic acid may be located at any position along either the sense or antisense strand. Nucleic acid molecules may include a group of about 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48 or 49 contiguous modified nucleotides. Modified nucleic acids may make up 1%, 2%, 3%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 97%, 98% or 100% of a nucleic acid strand. Modified nucleic acids of the examples immediately below may be in the sense strand only, the antisense strand only, or in both the sense and antisense strand.

General nucleic acid patters are shown below where X=sense strand nucleotide in the duplex region; X_(a)=5′-overhang nucleotide in the sense strand; X_(b)=3′-overhang nucleotide in the sense strand; Y=antisense strand nucleotide in the duplex region; Y_(a)=3′-overhang nucleotide in the antisense strand; Y_(b)=5′-overhang nucleotide in the antisense strand; and M=a modified nucleotide in the duplex region. Each a and b are independently 0 to 8 (e.g., 0, 1, 2, 3, 4, 5, 6, 7 or 8). Each X, Y, a and b are independently modified or unmodified. The sense and antisense strands can are each independently 17-49 nucleotides in length. The examples provided below have a duplex region of 19 nucleotides; however, nucleic acid molecules disclosed herein can have a duplex region anywhere between 17 and 49 nucleotides and where each strand is independently between 17 and 49 nucleotides in length.

5′ X_(a)XXXXXXXXXXXXXXXXXXXX_(b) 3′ Y_(b)YYYYYYYYYYYYYYYYYYYY_(a)

Further exemplary nucleic acid molecule patterns are shown below where X=unmodified sense strand nucleotides; x=an unmodified overhang nucleotide in the sense strand; Y=unmodified antisense strand nucleotides; y=an unmodified overhang nucleotide in the antisense strand; and M=a modified nucleotide. The sense and antisense strands can are each independently 17-49 nucleotides in length. The examples provided below have a duplex region of 19 nucleotides; however, nucleic acid molecules disclosed herein can have a duplex region anywhere between 17 and 49 nucleotides and where each strand is independently between 17 and 49 nucleotides in length.

5′ M _(n)XXXXXXXXXMXXXXXXXXXM _(n) 3′ M _(n)YYYYYYYYYYYYYYYYYYYM _(n) 5′ XXXXXXXXXXXXXXXXXXX 3′ YYYYYYYYYMYYYYYYYYY 5′ XXXXXXXXMMXXXXXXXXX 3′ YYYYYYYYYYYYYYYYYYY 5′ XXXXXXXXXXXXXXXXXXX 3′ YYYYYYYYMMYYYYYYYYY 5′ XXXXXXXXXMXXXXXXXXX 3′ YYYYYYYYYMYYYYYYYYY 5′ XXXXXMXXXXXXXXXXXXX 3′ YYYYYYYYYMYYYYYYYYY 5′ MXXXXXXXXXXXXXXXXXX 3′ YYYYYYYYYYYYMYYYYYY 5′ XXXXXXXXXXXXXXXXXXM 3′ YYYYYMYYYYYYYYYYYYY 5′ XXXXXXXXXMXXXXXXXX 3′ MYYYYYYYYYYYYYYYYY 5′ XXXXXXXMXXXXXXXXXX 3′ YYYYYYYYYYYYYYYYYM 5′ XXXXXXXXXXXXXMXXXX 3′ MYYYYYYYYYYYYYYYYY 5′ MMMMMMMMMMMMMMMMMM 3′ MMMMMMMMMMMMMMMMMM

Nucleic acid molecules may have blunt ends on both ends with alternating modified nucleic acids. The modified nucleic acids may be located at any position along either the sense or antisense strand.

5′ MXMXMXMXMXMXMXMXMXM 3′ YMYMYMYMYMYMYMYMYMY 5′ XMXMXMXMXMXMXMXMXMX 3′ MYMYMYMYMYMYMYMYMYM 5′ MMXMMXMMXMMXMMXMMXM 3′ YMMYMMYMMYMMYMMYMMY 5′ XMMXMMXMMXMMXMMXMMX 3′ MMYMMYMMYMMYMMYMMYM 5′ MMMXMMMXMMMXMMMXMMM 3′ YMMMYMMMYMMMYMMMYMM 5′ XMMMXMMMXMMMXMMMXMM 3′ MMMYMMMYMMMYMMMYMMM

Nucleic acid molecules with a blunt 5′-end and 3′-end overhang end with a single modified nucleic acid.

Nucleic acid molecules with a 5′-end overhang and a blunt 3′-end with a single modified nucleic acid.

Nucleic acid molecules with overhangs on both ends and all overhangs are modified nucleic acids. In the pattern immediately below, M is n number of modified nucleic acids, where n is an integer from 0 to 8 (i.e., 0, 1, 2, 3, 4, 5, 6, 7 and 8).

5′  XXXXXXXXXXXXXXXXXXXM 3′ MYYYYYYYYYYYYYYYYYYY

Nucleic acid molecules with overhangs on both ends and some overhang nucleotides are modified nucleotides. In the patterns immediately below, M is n number of modified nucleotides, x is n number of unmodified overhang nucleotides in the sense strand, y is n number of unmodified overhang nucleotides in the antisense strand, where each n is independently an integer from 0 to 8 (i.e., 0, 1, 2, 3, 4, 5, 6, 7 and 8), and where each overhang is maximum of 20 nucleotides; preferably a maximum of 8 nucleotides (modified and/or unmodified).

5′         XXXXXXXXXXXXXXXXXXXM 3′        yYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXMx 3′        yYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXMxM 3′        yYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXMxMx 3′        yYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXMxMxM 3′        yYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXMxMxMx 3′        yYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXMxMxMxM 3′        yYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXMxMxMxMx 3′        yYYYYYYYYYYYYYYYYYYY 5′        MXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYYy 5′       xMXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYYy 5′      MxMXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYYy 5′     xMxMXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYYy 5′    MxMxMXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYYy 5′   xMxMxMXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYYy 5′  MxMxMxMXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYYy 5′ xMxMxMxMXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYYy 5′        xXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYYM 5′        xXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYYMy 5′        xXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYYMyM 5′        xXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYYMyMy 5′        xXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYYMyMyM 5′        xXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYYMyMyMy 5′        xXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYYMyMyMyM 5′        xXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYYMyMyMyMy 5′         XXXXXXXXXXXXXXXXXXXx 3′        MYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXx 3′       yMYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXx 3′      MyMYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXx 3′     yMyMYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXx 3′    MyMyMYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXx 3′   yMyMyMYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXx 3′  MyMyMyMYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXx 3′ yMyMyMyMYYYYYYYYYYYYYYYYYYY Modified nucleotides at the 3′-end of the sense region. 5′         XXXXXXXXXXXXXXXXXXXM 3′         YYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXMM 3′         YYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXMMM 3′         YYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXMMMM 3′         YYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXMMMMM 3′         YYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXMMMMMM 3′         YYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXMMMMMMMM 3′         YYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXXMMMMMMMM 3′         YYYYYYYYYYYYYYYYYYY Overhang at the 5′-end of the sense region. 5′        MXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYY 5′       MMXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYY 5′      MMMXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYY 5′     MMMMXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYY 5′    MMMMMXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYY 5′   MMMMMMXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYY 5′  MMMMMMMXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYY 5′ MMMMMMMMXXXXXXXXXXXXXXXXXXX 3′         YYYYYYYYYYYYYYYYYYY Overhang at the 3′-end of the antisense region. 5′         XXXXXXXXXXXXXXXXXXX 3′        MYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXX 3′       MMYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXX 3′      MMMYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXX 3′     MMMMYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXX 3′    MMMMMYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXX 3′   MMMMMMYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXX 3′  MMMMMMMYYYYYYYYYYYYYYYYYYY 5′         XXXXXXXXXXXXXXXXXXX 3′ MMMMMMMMYYYYYYYYYYYYYYYYYYY Modified nucleotide(s) within the sense region 5′   XXXXXXXXXMXXXXXXXXX 3′   YYYYYYYYYYYYYYYYYYY 5′   XXXXXXXXXXXXXXXXXXX 3′   YYYYYYYYYMYYYYYYYYY 5′   XXXXXXXXXXXXXXXXXXXMM 3′   YYYYYYYYYYYYYYYYYYY 5′   XXXXXXXXXXXXXXXXXXX 3′ MMYYYYYYYYYYYYYYYYYYY

Exemplary nucleic acid molecules are provided below along with the equivalent general structure in line with the symbols used above:

siHSP47-C siRNA to human and rat hsp47 having a 19 nucleotide (i.e., 19 mer) duplex region and modified 2 nucleotide (i.e., deoxynucleotide) overhangs at the 3′-ends of the sense and antisense strands.

(SEQ ID NO: 5) 5′     GGACAGGCCUCUACAACUAdTdT 3′ (SEQ ID NO: 6) 3′ dTdTCCUGUCCGGAGAUGUUGAU 5′ 5′   XXXXXXXXXXXXXXXXXXXMM 3′ MMYYYYYYYYYYYYYYYYYYY

siHSP47-Cd siRNA to human and rat hsp47 having a 25-mer duplex region, a 2 nucleotide overhang at the 3′-end of the antisense strand and 2 modified nucleotides at the 5′-terminal and penultimate positions of the sense strand.

(SEQ ID NO: 9) 5′   GGACAGGCCUCUACAACUACUACdGdA 3′ (SEQ ID NO: 10) 3′ UUCCUGUCCGGAGAUGUUGAUGAUGCU   5′ 5′   XXXXXXXXXXXXXXXXXXXXXXXMM   3′ 3′ yyYYYYYYYYYYYYYYYYYYYYYYYYY   5′

siHSP47-1 siRNA to human and rat hsp47 cDNA 719-737 having a 19-mer duplex region, and modified 2 nucleotide (i.e., deoxynucleotide) overhangs at the 3′-ends of the sense and antisense strands.

(SEQ ID NO: 13) 5′     CAGGCCUCUACAACUACUAdTdT 3′ (SEQ ID NO: 14) 3′ dTdTGUCCGGAGAUGUUGAUGAU     5′ 5′   XXXXXXXXXXXXXXXXXXXMM     3′ 3′ MMYYYYYYYYYYYYYYYYYYY       5′

siHSP47-1d siRNA to human hsp47 cDNA 719-743 having a 25-mer with a blunt end at the 3′-end of the sense strand and a 2 nucleotide overhang at the 3′-end of the antisense strand, and 2 modified nucleotides at the 5′-terminal and penultimate positions of the sense strand.

(SEQ ID NO: 17) 5′   CAGGCCUCUACAACUACUACGACdGdA 3′ (SEQ ID NO: 2724) 3′ UUGUCCGGAGAUGUUGAUGAUGCUGCU   5′ 5′   XXXXXXXXXXXXXXXXXXXXXXXMM   3′ 3′ yyYYYYYYYYYYYYYYYYYYYYYYYYY   5′

siHSP47-2 siRNA to human hsp47 cDNA 469-487 having a 19-mer duplex region, and modified 2 nucleotide (i.e., deoxynucleotide) overhangs at the 3′-ends of the sense and antisense strands.

(SEQ ID NO:21) 5′     GAGCACUCCAAGAUCAACUdTdT 3′ (SEQ ID NO:22) 3′ dTdTCUCGUGAGGUUCUAGUUGA     5′ 5′     XXXXXXXXXXXXXXXXXXXMM   3′ 3′   MMYYYYYYYYYYYYYYYYYYY     5′

siHSP47-2d siRNA to human hsp47 cDNA 469-493 having a 25-mer duplex region with a blunt end at the 3′-end of the sense strand and a 2 nucleotide overhang at the 3′-end of the antisense strand, and 2 modified nucleotides at the 5′-terminal and penultimate positions of the sense strand.

(SEQ ID NO: 25) 5′   GAGCACUCCAAGAUCAACUUCCGdCdG 3′ (SEQ ID NO: 2725) 3′ UUCUCGUGAGGUUCUAGUUGAAGGCGC   5′ 5′   XXXXXXXXXXXXXXXXXXXXXXXMM   3′ 3′ yyYYYYYYYYYYYYYYYYYYYYYYYYY   5′

siHSP47-2d rat siRNA to rat Gp46 cDNA 466-490 having a 25-mer duplex region with a blunt end at the 3′-end of the sense strand and a 2 nucleotide overhang at the 3′-end of the antisense strand, and 2 modified nucleotides at the 5′-terminal and penultimate positions of the sense strand.

(SEQ ID NO: 29) 5′   GAACACUCCAAGAUCAACUUCCGdAdG 3′ (SEQ ID NO: 2726) 3′ UUCUUGUGAGGUUCUAGUUGAAGGCUC   5′ 5′   XXXXXXXXXXXXXXXXXXXXXXXMM   3′ 3′ yyYYYYYYYYYYYYYYYYYYYYYYYYY   5′

siHSP47-3 siRNA to human hsp47 cDNA 980-998 having a 19-mer duplex region, and modified 2 nucleotide (i.e., deoxynucleotide) overhangs at the 3′-ends of the sense and antisense strands.

(SEQ ID NO: 2727) 5′     CTGAGGCCATTGACAAGAAdTdT 3′ (SEQ ID NO: 34) 3′ dTdTGACUCCGGUAACUGUUCUU     5′ 5′     XXXXXXXXXXXXXXXXXXXMM   3′ 3′   MMYYYYYYYYYYYYYYYYYYY     5′

siHSP47-3d siRNA to human hsp47 cDNA 980-1004 having a 25-mer duplex region with a blunt end at the 3′-end of the sense strand and a 2 nucleotide overhang at the 3′-end of the antisense strand, and 2 modified nucleotides at the 5′-terminal and penultimate positions of the sense strand.

(SEQ ID NO: 2728) 5′   CTGAGGCCATTGACAAGAACAAGdGdC 3′ (SEQ ID NO: 38) 3′ UUGACUCCGGUAACUGUUCUUGUUCCG   5′ 5′   XXXXXXXXXXXXXXXXXXXXXXXMM   3′ 3′ yyYYYYYYYYYYYYYYYYYYYYYYYYY   5′

siHSP47-4 siRNA to human hsp47 cDNA 735-753 having a 19-mer duplex region, and modified 2 nucleotide (i.e., deoxynucleotide) overhangs at the 3′-ends of the sense and antisense strands.

(SEQ ID NO: 41) 5′     CUACGACGACGAGAAGGAAdTdT 3′ (SEQ ID NO: 42) 3′ dTdTGAUGCUGCUGCUCUUCCUU     5′ 5′     XXXXXXXXXXXXXXXXXXXMM   3′ 3′   MMYYYYYYYYYYYYYYYYYYY     5′

siHSP47-4d siRNA to human hsp47 cDNA 735-759 having a 25-mer duplex region with a blunt end at the 3′-end of the sense strand and a 2 nucleotide overhang at the 3′-end of the antisense strand, and 2 modified nucleotides at the 5′-terminal and penultimate positions of the sense strand.

(SEQ ID NO: 45) 5′   CUACGACGACGAGAAGGAAAAGCdTdG 3′ (SEQ ID NO: 2729) 3′ UUGAUGCUGCUGCUCUUCCUUUUCGAC   5′ 5′   XXXXXXXXXXXXXXXXXXXXXXXMM   3′ 3′ yyYYYYYYYYYYYYYYYYYYYYYYYYY   5′

siHSP47-5 siRNA to human hsp47 cDNA 621-639 having a 19-mer duplex region, and modified 2 nucleotide (i.e., deoxynucleotide) overhangs at the 3′-ends of the sense and antisense strands.

(SEQ ID NO: 49) 5′     GCCACACUGGGAUGAGAAAdTdT 3′ (SEQ ID NO: 50) 3′ dTdTCGGUGUGACCCUACUCUUU 5′ 5′     XXXXXXXXXXXXXXXXXXXMM 3′ 3′   MMYYYYYYYYYYYYYYYYYYY 5′

siHSP47-6 siRNA to human hsp47 cDNA 446-464 having a 19-mer duplex region, and modified 2 nucleotide (i.e., deoxynucleotide) overhangs at the 3′-ends of the sense and antisense strands.

(SEQ ID NO: 53) 5′     GCAGCAAGCAGCACUACAAdTdT 3′ (SEQ ID NO: 54) 3′ dTdTCGUCGUUCGUCGUGAUGUU 5′ 5′     XXXXXXXXXXXXXXXXXXXMM 3′ 3′   MMYYYYYYYYYYYYYYYYYYY 5′

siHSP47-7 siRNA to human hsp47 cDNA 692-710 having a 19-mer duplex region, and modified 2 nucleotide (i.e., deoxynucleotide) overhangs at the 3′-ends of the sense and antisense strands.

(SEQ ID NO: 57) 5′     CCGUGGGUGUCAUGAUGAUdTdT 3′ (SEQ ID NO: 58) 3′ dTdTGGCACCCACAGUACUACUA 5′ 5′     XXXXXXXXXXXXXXXXXXXMM 3′ 3′   MMYYYYYYYYYYYYYYYYYYY 5′

Nicks and Gaps in Nucleic Acid Strands

Nucleic acid molecules (e.g., siNA molecules) provided herein may have a strand, preferably, the sense strand, that is nicked or gapped. As such, nucleic acid molecules may have three or more strand, for example, such as a meroduplex RNA (mdRNA) disclosed in PCT/US07/081836. Nucleic acid molecules with a nicked or gapped strand may be between about 1-49 nucleotides, or may be RISC length (e.g., about 15 to 25 nucleotides) or Dicer substrate length (e.g., about 25 to 30 nucleotides) such as disclosed herein.

Nucleic acid molecules with three or more strands include, for example, an ‘A’ (antisense) strand, ‘S1’ (second) strand, and ‘S2’ (third) strand in which the ‘S1’ and ‘S2’ strands are complementary to and form base pairs with non-overlapping regions of the ‘A’ strand (e.g., an mdRNA can have the form of A:S1S2). The S1, S2, or more strands together form what is substantially similar to a sense strand to the ‘A’ antisense strand. The double-stranded region formed by the annealing of the ‘S1’ and ‘A’ strands is distinct from and non-overlapping with the double-stranded region formed by the annealing of the ‘S2’ and ‘A’ strands. A nucleic acid molecule (e.g., an siNA molecule) may be a “gapped” molecule, meaning a “gap” ranging from 0 nucleotides up to about 10 nucleotides (e.g., 0, 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 nucleotides). Preferably, the sense strand is gapped. In some embodiments, the A:S1 duplex is separated from the A:S2 duplex by a gap resulting from at least one unpaired nucleotide (up to about 10 unpaired nucleotides) in the ‘A’ strand that is positioned between the A:S1 duplex and the A:S2 duplex and that is distinct from any one or more unpaired nucleotide at the 3′-end of one or more of the ‘A’, ‘S1’, or ‘S2 strands. The A:S1 duplex may be separated from the A:B2 duplex by a gap of zero nucleotides (i.e., a nick in which only a phosphodiester bond between two nucleotides is broken or missing in the polynucleotide molecule) between the A:S1 duplex and the A:S2 duplex-which can also be referred to as nicked dsRNA (ndsRNA). For example, A:S1S2 may be include a dsRNA having at least two double-stranded regions that combined total about 14 base pairs to about 40 base pairs and the double-stranded regions are separated by a gap of about 0 to about 10 nucleotides, optionally having blunt ends, or A:S1S2 may include a dsRNA having at least two double-stranded regions separated by a gap of up to ten nucleotides wherein at least one of the double-stranded regions includes between about five base pairs and thirteen base pairs.

Dicer Substrates

In certain embodiments, the nucleic acid molecules (e.g., siNA molecules) provided herein may be a precursor “Dicer substrate” molecule, e.g., double-stranded nucleic acid, processed in vivo to produce an active nucleic acid molecules, for example as described in Rossi, US20050244858. In certain conditions and situations, it has been found that these relatively longer dsRNA siNA species, e.g., of from about 25 to about 30 nucleotides, can give unexpectedly effective results in terms of potency and duration of action. Without wishing to be bound by any particular theory, it is thought that the longer dsRNA species serve as a substrate for the enzyme Dicer in the cytoplasm of a cell. In addition to cleaving double-stranded nucleic acid into shorter segments, Dicer may facilitate the incorporation of a single-stranded cleavage product derived from the cleaved dsRNA into the RNA-induced silencing complex (RISC complex) that is responsible for the destruction of the cytoplasmic RNA derived from the target gene.

Dicer substrates may have certain properties which enhance its processing by Dicer. Dicer substrates are of a length sufficient such that it is processed by Dicer to produce an active nucleic acid molecule and may further include one or more of the following properties: (i) the dsRNA is asymmetric, e.g., has a 3′-overhang on the first strand (antisense strand) and (ii) the dsRNA has a modified 3′-end on the antisense strand (sense strand) to direct orientation of Dicer binding and processing of the dsRNA to an active siRNA. In certain embodiments, the longest strand in the Dicer substrate may be 24-30 nucleotides.

Dicer substrates may be symmetric or asymmetric. The Dicer substrate may have a sense strand includes 22-28 nucleotides and the antisense strand may include 24-30 nucleotides; thus, in some embodiments the resulting Dicer substrate may have an overhang on the 3′ end of the antisense strand. Dicer substrate may have a sense strand 25 nucleotides in length, and the antisense strand having 27 nucleotides in length with a two base 3′-overhang. The overhang may be 1-3 nucleotides, for example 2 nucleotides. The sense strand may also have a 5′-phosphate.

An asymmetric Dicer substrate may further contain two deoxynucleotides at the 3′-end of the sense strand in place of two of the ribonucleotides. Some exemplary Dicer substrates lengths and structures are 21+0, 21+2, 21−2, 22+0, 22+1, 22−1, 23+0, 23+2, 23−2, 24+0, 24+2, 24−2, 25+0, 25+2, 25−2, 26+0, 26+2, 26−2, 27+0, 27+2, and 27−2.

The sense strand of a Dicer substrate may be between about 22 to about 30 (e.g., about 22, 23, 24, 25, 26, 27, 28, 29 or 30); about 22 to about 28; about 24 to about 30; about 25 to about 30; about 26 to about 30; about 26 and 29; or about 27 to about 28 nucleotides in length. In certain preferred embodiments Dicer substrates contain sense and antisense strands, that are at least about 25 nucleotides in length and no longer than about 30 nucleotides; between about 26 and 29 nucleotides; or 27 nucleotides in length. The sense and antisense strands may be the same length (blunt ended), different lengths (have overhangs), or a combination. The sense and antisense strands may exist on the same polynucleotide or on different polynucleotides. A Dicer substrate may have a duplex region of about 19, 20, 21, 22, 23, 24, 25 or 27 nucleotides.

Like other siNA molecules provided herein, the antisense strand of a Dicer substrate may have any sequence that anneals to the antisense strand under biological conditions, such as within the cytoplasm of a eukaryotic cell.

Dicer substrates may have any modifications to the nucleotide base, sugar or phosphate backbone as known in the art and/or as described herein for other nucleic acid molecules (such as siNA molecules). In certain embodiments, Dicer substrates may have a sense strand is modified for Dicer processing by suitable modifiers located at the 3′-end of the sense strand, i.e., the dsRNA is designed to direct orientation of Dicer binding and processing. Suitable modifiers include nucleotides such as deoxyribonucleotides, dideoxyribonucleotides, acyclo-nucleotides and the like and sterically hindered molecules, such as fluorescent molecules and the like. Acyclo-nucleotides substitute a 2-hydroxyethoxymethyl group for—the 2′-deoxyribofuranosyl sugar normally present in dNMPs. Other nucleotides modifiers that could be used in Dicer substrate siNA molecules include 3′-deoxyadenosine (cordycepin), 3′-azido-3′-deoxythymidine (AZT), 2′,3′-dideoxyinosine (ddI), 2′,3′-dideoxy-3′-thiacytidine (3TC), 2′,3′-didehydro-2′,3′-dideoxythymidine (d4T) and the monophosphate nucleotides of 3′-azido-3′-deoxythymidine (AZT), 2′,3′-dideoxy-3′-thiacytidine (3TC) and 2′,3′-didehydro-2′,3′-dideoxythymidine (d4T). In one embodiment, deoxynucleotides are used as the modifiers. When nucleotide modifiers are utilized, they may replace ribonucleotides (e.g., 1-3 nucleotide modifiers, or 2 nucleotide modifiers are substituted for the ribonucleotides on the 3′-end of the sense strand) such that the length of the Dicer substrate does not change. When sterically hindered molecules are utilized, they may be attached to the ribonucleotide at the 3′-end of the antisense strand. Thus, in certain embodiments the length of the strand does not change with the incorporation of the modifiers. In certain embodiments, two DNA bases in the dsRNA are substituted to direct the orientation of Dicer processing of the antisense strand. In a further embodiment of, two terminal DNA bases are substituted for two ribonucleotides on the 3′-end of the sense strand forming a blunt end of the duplex on the 3′-end of the sense strand and the 5′-end of the antisense strand, and a two-nucleotide RNA overhang is located on the 3′-end of the antisense strand. This is an asymmetric composition with DNA on the blunt end and RNA bases on the overhanging end.

In certain embodiments modifications are included in the Dicer substrate such that the modification does not prevent the nucleic acid molecule from serving as a substrate for Dicer. In one embodiment, one or more modifications are made that enhance Dicer processing of the Dicer substrate. One or more modifications may be made that result in more effective RNAi generation. One or more modifications may be made that support a greater RNAi effect. One or more modifications are made that result in greater potency per each Dicer substrate to be delivered to the cell. Modifications may be incorporated in the 3′-terminal region, the 5′-terminal region, in both the 3′-terminal and 5′-terminal region or at various positions within the sequence. Any number and combination of modifications can be incorporated into the Dicer substrate so long as the modification does not prevent the nucleic acid molecule from serving as a substrate for Dicer. Where multiple modifications are present, they may be the same or different. Modifications to bases, sugar moieties, the phosphate backbone, and their combinations are contemplated. Either 5′-terminus can be phosphorylated.

Examples of Dicer substrate phosphate backbone modifications include phosphonates, including methylphosphonate, phosphorothioate, and phosphotriester modifications such as alkylphosphotriesters, and the like. Examples of Dicer substrate sugar moiety modifications include 2′-alkyl pyrimidine, such as 2′OMe, 2′-fluoro, amino, and deoxy modifications and the like (see, e.g., Amarzguioui et al., 2003). Examples of Dicer substrate base group modifications include abasic sugars, 2-O-alkyl modified pyrimidines, 4-thiouracil, 5-bromouracil, 5-iodouracil, and 5-(3-aminoallyl)-uracil and the like. LNAs could also be incorporated.

The sense strand may be modified for Dicer processing by suitable modifiers located at the 3′-end of the sense strand, i.e., the Dicer substrate is designed to direct orientation of Dicer binding and processing. Suitable modifiers include nucleotides such as deoxyribonucleotides, dideoxyribonucleotides, acyclo-nucleotides and the like and sterically hindered molecules, such as fluorescent molecules and the like. Acyclo-nucleotides substitute a 2-hydroxyethoxymethyl group for—the 2′-deoxyribofuranosyl sugar normally present in dNMPs. Other nucleotides modifiers could include cordycepin, AZT, ddI, 3TC, d4T and the monophosphate nucleotides of AZT, 3TC and d4T. In one embodiment, deoxynucleotides are used as the modifiers. When nucleotide modifiers are utilized, 1-3 nucleotide modifiers, or 2 nucleotide modifiers are substituted for the ribonucleotides on the 3′-end of the sense strand. When sterically hindered molecules are utilized, they are attached to the ribonucleotide at the 3′-end of the antisense strand. Thus, the length of the strand does not change with the incorporation of the modifiers. In another embodiment, the description contemplates substituting two DNA bases in the Dicer substrate to direct the orientation of Dicer processing of the antisense strand. In a further embodiment of the present description, two terminal DNA bases are substituted for two ribonucleotides on the 3′-end of the sense strand forming a blunt end of the duplex on the 3′-end of the sense strand and the 5′-end of the antisense strand, and a two-nucleotide RNA overhang is located on the 3′-end of the antisense strand. This is an asymmetric composition with DNA on the blunt end and RNA bases on the overhanging end.

The antisense strand may be modified for Dicer processing by suitable modifiers located at the 3′-end of the antisense strand, i.e., the dsRNA is designed to direct orientation of Dicer binding and processing. Suitable modifiers include nucleotides such as deoxyribonucleotides, dideoxyribonucleotides, acyclo-nucleotides and the like and sterically hindered molecules, such as fluorescent molecules and the like. Acyclo-nucleotides substitute a 2-hydroxyethoxymethyl group for the 2′-deoxyribofuranosyl sugar normally present in dNMPs. Other nucleotides modifiers could include cordycepin, AZT, ddI, 3TC, d4T and the monophosphate nucleotides of AZT, 3TC and d4T. In one embodiment, deoxynucleotides are used as the modifiers. When nucleotide modifiers are utilized, 1-3 nucleotide modifiers, or 2 nucleotide modifiers are substituted for the ribonucleotides on the 3′-end of the antisense strand. When sterically hindered molecules are utilized, they are attached to the ribonucleotide at the 3′-end of the antisense strand. Thus, the length of the strand does not change with the incorporation of the modifiers. In another embodiment, the description contemplates substituting two DNA bases in the dsRNA to direct the orientation of Dicer processing. In a further description, two terminal DNA bases are located on the 3′-end of the antisense strand in place of two ribonucleotides forming a blunt end of the duplex on the 5′-end of the sense strand and the 3′-end of the antisense strand, and a two-nucleotide RNA overhang is located on the 3′-end of the sense strand. This is an asymmetric composition with DNA on the blunt end and RNA bases on the overhanging end.

Dicer substrates with a sense and an antisense strand can be linked by a third structure. The third structure will not block Dicer activity on the Dicer substrate and will not interfere with the directed destruction of the RNA transcribed from the target gene. The third structure may be a chemical linking group. Suitable chemical linking groups are known in the art and can be used. Alternatively, the third structure may be an oligonucleotide that links the two oligonucleotides of the dsRNA is a manner such that a hairpin structure is produced upon annealing of the two oligonucleotides making up the Dicer substrate. The hairpin structure preferably does not block Dicer activity on the Dicer substrate or interfere with the directed destruction of the RNA transcribed from the target gene.

The sense and antisense strands of the Dicer substrate are not required to be completely complementary. They only need to be substantially complementary to anneal under biological conditions and to provide a substrate for Dicer that produces a siRNA sufficiently complementary to the target sequence.

Dicer substrate can have certain properties that enhance its processing by Dicer. The Dicer substrate can have a length sufficient such that it is processed by Dicer to produce an active nucleic acid molecules (e.g., siRNA) and may have one or more of the following properties: the Dicer substrate is asymmetric, e.g., has a 3′-overhang on the first strand (antisense strand) and/or the Dicer substrate has a modified 3′ end on the second strand (sense strand) to direct orientation of Dicer binding and processing of the Dicer substrate to an active siRNA. The Dicer substrate can be asymmetric such that the sense strand includes 22-28 nucleotides and the antisense strand includes 24-30 nucleotides. Thus, the resulting Dicer substrate has an overhang on the 3′ end of the antisense strand. The overhang is 1-3 nucleotides, for example two nucleotides. The sense strand may also have a 5′ phosphate.

A Dicer substrate may have an overhang on the 3′-end of the antisense strand and the sense strand is modified for Dicer processing. The 5′-end of the sense strand may have a phosphate. The sense and antisense strands may anneal under biological conditions, such as the conditions found in the cytoplasm of a cell. A region of one of the strands, particularly the antisense strand, of the Dicer substrate may have a sequence length of at least 19 nucleotides, wherein these nucleotides are in the 21-nucleotide region adjacent to the 3′-end of the antisense strand and are sufficiently complementary to a nucleotide sequence of the RNA produced from the target gene. A Dicer substrate may also have one or more of the following additional properties: the antisense strand has a right shift from a corresponding 21-mer (i.e., the antisense strand includes nucleotides on the right side of the molecule when compared to the corresponding 21-mer), the strands may not be completely complementary, i.e., the strands may contain simple mismatch pairings and base modifications such as LNA may be included in the 5′-end of the sense strand.

An antisense strand of a Dicer substrate nucleic acid molecule may be modified to include 1-9 ribonucleotides on the 5′-end to give a length of 22-28 nucleotides. When the antisense strand has a length of 21 nucleotides, then 1-7 ribonucleotides, or 2-5 ribonucleotides and or 4 ribonucleotides may be added on the 3′-end. The added ribonucleotides may have any sequence. Although the added ribonucleotides may be complementary to the target gene sequence, full complementarity between the target sequence and the antisense strands is not required. That is, the resultant antisense strand is sufficiently complementary with the target sequence. A sense strand may then have 24-30 nucleotides. The sense strand may be substantially complementary with the antisense strand to anneal to the antisense strand under biological conditions. In one embodiment, the antisense strand may be synthesized to contain a modified 3′-end to direct Dicer processing. The sense strand may have a 3′ overhang. The antisense strand may be synthesized to contain a modified 3′-end for Dicer binding and processing and the sense strand has a 3′ overhang.

Heat Shock Protein 47

Heat shock protein 47 (HSP47) is a collagen-specific molecular chaperone and resides in the endoplasmic reticulum. It interacts with procollagen during the process of folding, assembling and transporting from the endoplasmic reticulum (Nagata Trends Biochem Sci 1996; 21:22-6; Razzaque et al. 2005; Contrib Nephrol 2005; 148: 57-69; Koide et al. 2006 J. Biol. Chem.; 281: 3432-38; Leivo et al. Dev. Biol. 1980; 76:100-114; Masuda et al. J. Clin. Invest. 1994; 94:2481-2488; Masuda et al. Cell Stress Chaperones 1998; 3:256-264). HSP47 has been reported to have an upregulated expression in various tissue fibrosis (Koide et al. J Biol Chem 1999; 274: 34523-26), such as liver cirrhosis (Masuda et al. J Clin Invest 1994; 94:2481-8), pulmonary fibrosis (Razzaque et al. Virchows Arch 1998; 432:455-60; Kakugawa et al. Eur Respir J 2004; 24: 57-65), and glomerulosclerosis (Moriyama et al. Kidney Int 1998; 54: 110-19). Exemplary nucleic acid sequence of target human hsp47 cDNA is disclosed in GenBank accession number: NM 001235 and the corresponding mRNA sequence, for example as listed as SEQ ID NO:1. One of ordinary skill in the art would understand that a given sequence may change over time and to incorporate any changes needed in the nucleic acid molecules herein accordingly.

The specific association of HSP47 with a diverse range of collagen types makes HSP47 a potential target for the treatment of fibrosis. Inhibition of hsp47 expression may prevent extracellular collagen I secretion. Sato et al. (Nat Biotechnol 2008; 26:431-442) explored this possibility by using siRNA for the inhibition hsp47 expression and preventing the progression of hepatic fibrosis in rats. Similarly, Chen et al. (Br J Dermatol 2007; 156: 1188-1195) and Wang et al. (Plast. Reconstr Surg 2003; 111: 1980-7) investigated the inhibition hsp47 expression by RNA interference technology.

Methods and Compositions for Inhibiting hsp47

Provided are compositions and methods for inhibition of hsp47 expression by using small nucleic acid molecules, such as short interfering nucleic acid (siNA), interfering RNA (RNAi), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules capable of mediating or that mediate RNA interference against hsp47 gene expression. The composition and methods disclosed herein are also useful in treating various fibrosis such as liver fibrosis, lung fibrosis, and kidney fibrosis.

Nucleic acid molecule(s) and/or methods of the description are used to down regulate the expression of gene(s) that encode RNA referred to, by example, Genbank Accession NM_(—)001235.

Compositions, methods and kits provided herein may include one or more nucleic acid molecules (e.g., siNA) and methods that independently or in combination modulate (e.g., downregulate) the expression of hsp47 protein and/or genes encoding hsp47 proteins, proteins and/or genes encoding hsp47 (e.g., genes encoding sequences comprising those sequences referred to by GenBank Accession Nos. NM_(—)001235), or an hsp47 gene family member where the genes or gene family sequences share sequence homology associated with the maintenance and/or development of diseases, conditions or disorders associated with hsp47, such as liver fibrosis, cirrhosis, pulmonary fibrosis, kidney fibrosis, peritoneal fibrosis, chronic hepatic damage, and fibrillogenesis. The description of the various aspects and embodiments is provided with reference to exemplary gene hsp47. However, the various aspects and embodiments are also directed to other related hsp47 genes, such as homolog genes and transcript variants, and polymorphisms (e.g., single nucleotide polymorphism, (SNPs)) associated with certain hsp47 genes. As such, the various aspects and embodiments are also directed to other genes that are involved in hsp47 mediated pathways of signal transduction or gene expression that are involved, for example, in the maintenance or development of diseases, traits, or conditions described herein. These additional genes can be analyzed for target sites using the methods described for the hsp47 gene herein. Thus, the modulation of other genes and the effects of such modulation of the other genes can be performed, determined, and measured as described herein.

In one embodiment, compositions and methods provided herein include a double-stranded short interfering nucleic acid (siNA) molecule that down-regulates expression of a hsp47 gene (e.g., human hsp47 exemplified by SEQ ID NO:1), where the nucleic acid molecule includes about 15 to about 49 base pairs.

In one embodiment, a nucleic acid disclosed may be used to inhibit the expression of the hsp47 gene or an hsp47 gene family where the genes or gene family sequences share sequence homology. Such homologous sequences can be identified as is known in the art, for example using sequence alignments. Nucleic acid molecules can be designed to target such homologous sequences, for example using perfectly complementary sequences or by incorporating non-canonical base pairs, for example mismatches and/or wobble base pairs that can provide additional target sequences. In instances where mismatches are identified, non-canonical base pairs (for example, mismatches and/or wobble bases) can be used to generate nucleic acid molecules that target more than one gene sequence. In a non-limiting example, non-canonical base pairs such as UU and CC base pairs are used to generate nucleic acid molecules that are capable of targeting sequences for differing hsp47 targets that share sequence homology. As such, one advantage of using siNAs disclosed herein is that a single nucleic acid can be designed to include nucleic acid sequence that is complementary to the nucleotide sequence that is conserved between the homologous genes. In this approach, a single nucleic acid can be used to inhibit expression of more than one gene instead of using more than one nucleic acid molecule to target the different genes.

Nucleic acid molecules may be used to target conserved sequences corresponding to a gene family or gene families such as hsp47 family genes. As such, nucleic acid molecules targeting multiple hsp47 targets can provide increased therapeutic effect. In addition, nucleic acid can be used to characterize pathways of gene function in a variety of applications. For example, nucleic acid molecules can be used to inhibit the activity of target gene(s) in a pathway to determine the function of uncharacterized gene(s) in gene function analysis, mRNA function analysis, or translational analysis. The nucleic acid molecules can be used to determine potential target gene pathways involved in various diseases and conditions toward pharmaceutical development. The nucleic acid molecules can be used to understand pathways of gene expression involved in, for example fibroses such as liver, kidney or pulmonary fibrosis, and/or inflammatory and proliferative traits, diseases, disorders, and/or conditions.

In one embodiment, the compositions and methods provided herein include a nucleic acid molecule having RNAi activity against hsp47 RNA, where the nucleic acid molecule includes a sequence complementary to any RNA having hsp47 encoding sequence, such as those sequences having sequences as shown in Table 3. In another embodiment, a nucleic acid molecule may have RNAi activity against hsp47 RNA, where the nucleic acid molecule includes a sequence complementary to an RNA having variant hsp47 encoding sequence, for example other mutant hsp47 genes not shown in Table 3 but known in the art to be associated with the maintenance and/or development of fibrosis. Chemical modifications as shown in Table 3 or otherwise described herein can be applied to any nucleic acid construct disclosed herein. In another embodiment, a nucleic acid molecule disclosed herein includes a nucleotide sequence that can interact with nucleotide sequence of a hsp47 gene and thereby mediate silencing of hsp47 gene expression, for example, wherein the nucleic acid molecule mediates regulation of hsp47 gene expression by cellular processes that modulate the chromatin structure or methylation patterns of the hsp47 gene and prevent transcription of the hsp47 gene.

Nucleic acid molecules disclosed herein may have RNAi activity against hsp47 RNA, where the nucleic acid molecule includes a sequence complementary to any RNA having hsp47 encoding sequence, such as those sequences having GenBank Accession Nos. NM_(—)001235. Nucleic acid molecules may have RNAi activity against hsp47 RNA, where the nucleic acid molecule includes a sequence complementary to an RNA having variant hsp47 encoding sequence, for example other mutant hsp47 genes known in the art to be associated with the maintenance and/or development of fibrosis.

Nucleic acid molecules disclosed herein include a nucleotide sequence that can interact with nucleotide sequence of a hsp47 gene and thereby mediate silencing of hsp47 gene expression, e.g., where the nucleic acid molecule mediates regulation of hsp47 gene expression by cellular processes that modulate the chromatin structure or methylation patterns of the hsp47 gene and prevent transcription of the hsp47 gene.

Methods of Treatment

The specific association of HSP47 with a diverse range of collagen types makes hsp47 a target for the treatment of fibrosis. Inhibition of hsp47 expression may prevent extracellular collagen I secretion. Sato et al. (Nat Biotechnol 2008; 26:431-442) explored this possibility by using siRNA for the inhibition hsp47 expression and preventing the progression of hepatic fibrosis in rats. Similarly, Chen et al. (Br J Dermatol 2007; 156: 1188-1195) and Wang et al. (Plast Reconstr Surg 2003; 111: 1980-7) investigated the inhibition hsp47 expression by RNA interference technology.

In one embodiment, nucleic acid molecules may be used to down regulate or inhibit the expression of hsp47 and/or hsp47 proteins arising from hsp47 and/or hsp47 haplotype polymorphisms that are associated with a disease or condition, (e.g., fibrosis). Analysis of hsp47 and/or hsp47 genes, or hsp47 and/or hsp47 protein or RNA levels can be used to identify subjects with such polymorphisms or those subjects who are at risk of developing traits, conditions, or diseases described herein. These subjects are amenable to treatment, for example, treatment with nucleic acid molecules disclosed herein and any other composition useful in treating diseases related to hsp47 and/or hsp47 gene expression. As such, analysis of hsp47 and/or hsp47 protein or RNA levels can be used to determine treatment type and the course of therapy in treating a subject. Monitoring of hsp47 and/or hsp47 protein or RNA levels can be used to predict treatment outcome and to determine the efficacy of compounds and compositions that modulate the level and/or activity of certain hsp47 and/or hsp47 proteins associated with a trait, condition, or disease.

Provided are compositions and methods for inhibition of hsp47 expression by using small nucleic acid molecules as provided herein, such as siNA, RNAi, siRNA, double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules capable of mediating or that mediate RNA interference against hsp47 gene expression. The composition and methods disclosed herein are also useful in treating various fibrosis such as liver fibrosis, lung fibrosis, and kidney fibrosis.

The nucleic acid molecules disclosed herein individually, or in combination or in conjunction with other drugs, can be used for preventing or treating diseases, traits, conditions and/or disorders associated with hsp47, such as liver fibrosis, cirrhosis, pulmonary fibrosis, kidney fibrosis, peritoneal fibrosis, chronic hepatic damage, and fibrillogenesis.

The nucleic acid molecules disclosed herein are able to inhibit the expression of hsp47 in a sequence specific manner. The nucleic acid molecules may include a sense strand and an antisense strand which include contiguous nucleotides that are at least partially complementary (antisense) to an hsp47 mRNA.

In some embodiments, dsRNA specific for hsp47 can be used in conjunction with other dsRNA specific for other molecular chaperones that assist in the folding of newly synthesized proteins such as, calnexin, calreticulin, and/or BiP (Bergeron et al. Trends Biochem. Sci. 1994; 19:124-128; Herbert et al. 1995; Cold Spring Harb. Symp. Quant. Biol. 60:405-415)

Fibrosis can be treated by RNA interference using nucleic acid molecules as disclosed herein. Exemplary fibroses include liver fibrosis, peritoneal fibrosis, lung fibrosis, kidney fibrosis. The nucleic acid molecules disclosed herein may inhibit the expression of hsp47 in a sequence specific manner.

Treatment of fibrosis can be monitored by determining the level of extracellular collagen using suitable techniques known in the art such as, using anti-collagen I antibodies. Treatment can also be monitored by determining the level of hsp47 mRNA or the level of HSP47 protein in the cells of the affected tissue. Treatment can also be monitored by non-invasive scanning of the affected organ or tissue such as by computer assisted tomography scan, magnetic resonance elastography scans.

A method for treating or preventing hsp47 associated disease or condition in a subject or organism may include contacting the subject or organism with a nucleic acid molecule as provided herein under conditions suitable to modulate the expression of the hsp47 gene in the subject or organism.

A method for treating or preventing fibrosis in a subject or organism may include contacting the subject or organism with a nucleic acid molecule under conditions suitable to modulate the expression of the hsp47 gene in the subject or organism.

A method for treating or preventing one or more fibroses selected from the group consisting of liver fibrosis, kidney fibrosis, and pulmonary fibrosis in a subject or organism may include contacting the subject or organism with a nucleic acid molecule under conditions suitable to modulate the expression of the hsp47 gene in the subject or organism.

Fibrotic Diseases

Fibrotic diseases are generally characterized by the excess deposition of a fibrous material within the extracellular matrix, which contributes to abnormal changes in tissue architecture and interferes with normal organ function.

All tissues damaged by trauma respond by the initiation of a wound-healing program. Fibrosis, a type of disorder characterized by excessive scarring, occurs when the normal self-limiting process of wound healing response is disturbed, and causes excessive production and deposition of collagen. As a result, normal organ tissue is replaced with scar tissue, which eventually leads to the functional failure of the organ.

Fibrosis may be initiated by diverse causes and in various organs. Liver cirrhosis, pulmonary fibrosis, sarcoidosis, keloids and kidney fibrosis are all chronic conditions associated with progressive fibrosis, thereby causing a continuous loss of normal tissue function.

Acute fibrosis (usually with a sudden and severe onset and of short duration) occurs as a common response to various forms of trauma including accidental injuries (particularly injuries to the spine and central nervous system), infections, surgery, ischemic illness (e.g. cardiac scarring following heart attack), burns, environmental pollutants, alcohol and other types of toxins, acute respiratory distress syndrome, radiation and chemotherapy treatments).

Fibrosis, a fibrosis related pathology or a pathology related to aberrant crosslinking of cellular proteins may all be treated by the siRNAs disclosed herein. Fibrotic diseases or diseases in which fibrosis is evident (fibrosis related pathology) include both acute and chronic forms of fibrosis of organs, including all etiological variants of the following pulmonary fibrosis, including interstitial lung disease and fibrotic lung disease, liver fibrosis, cardiac fibrosis including myocardial fibrosis, kidney fibrosis including chronic renal failure, skin fibrosis including scleroderma, keloids and hypertrophic scars; myelofibrosis (bone marrow fibrosis); all types of ocular scarring including proliferative vitreoretinopathy (PVR) and scarring resulting from surgery to treat cataract or glaucoma; inflammatory bowel disease of variable etiology, macular degeneration, Grave's ophthalmopathy, drug induced ergotism, keloid scars, scleroderma, psoriasis, glioblastoma in Li-Fraumeni syndrome, sporadic glioblastoma, myleoid leukemia, acute myelogenous leukemia, myelodysplastic syndrome, myeloproferative syndrome, gynecological cancer, Kaposi's sarcoma, Hansen's disease, and collagenous colitis.

In various embodiments, the compounds (nucleic acid molecules) as disclosed herein may be used to treat fibrotic diseases, for example as disclosed herein, as well as many other diseases and conditions apart from fibrotic diseases, for example such as disclosed herein. Other conditions to be treated include fibrotic diseases in other organs—kidney fibrosis for any reason (CKD including ESRD); lung fibrosis (including ILF); myelofibrosis, abnormal scarring (keloids) associated with all possible types of skin injury accidental and jatrogenic (operations); scleroderma; cardiofibrosis, failure of glaucoma filtering operation; intestinal adhesions.

Ocular Surgery and Fibrotic Complications

Contracture of scar tissue resulting from eye surgery may often occur. Glaucoma surgery to create new drainage channels often fails due to scarring and contraction of tissues and the generated drainage system may be blocked requiring additional surgical intervention. Current anti-scarring regimens (Mitomycin C or 5FU) are limited due to the complications involved (e.g. blindness) e.g. see Cordeiro M F, et al., Human anti-transforming growth factor-beta2 antibody: a new glaucoma anti-scarring agent Invest Ophthalmol Vis Sci. 1999 September; 40(10):2225-34. There may also be contraction of scar tissue formed after corneal trauma or corneal surgery, for example laser or surgical treatment for myopia or refractive error in which contraction of tissues may lead to inaccurate results. Scar tissue may be formed on/in the vitreous humor or the retina, for example, and may eventually causes blindness in some diabetics, and may be formed after detachment surgery, called proliferative vitreoretinopathy (PVR). PVR is the most common complication following retinal detachment and is associated with a retinal hole or break. PVR refers to the growth of cellular membranes within the vitreous cavity and on the front and back surfaces of the retina containing retinal pigment epithelial (RPE) cells. These membranes, which are essentially scar tissues, exert traction on the retina and may result in recurrences of retinal detachment, even after an initially successful retinal detachment procedure.

Scar tissue may be formed in the orbit or on eye and eyelid muscles after squint, orbital or eyelid surgery, or thyroid eye disease, and where scarring of the conjunctiva occurs as may happen after glaucoma surgery or in cicatricial disease, inflammatory disease, for example, pemphigoid, or infective disease, for example, trachoma. A further eye problem associated with the contraction of collagen-including tissues is the opacification and contracture of the lens capsule after cataract extraction. Important role for MMPs has been recognized in ocular diseases including wound healing, dry eye, sterile corneal ulceration, recurrent epithelial erosion, corneal neovascularization, pterygium, conjuctivochalasis, glaucoma, PVR, and ocular fibrosis.

Liver Fibrosis

Liver fibrosis (LF) is a generally irreversible consequence of hepatic damage of several etiologies. In the Western world, the main etiologic categories are: alcoholic liver disease (30-50%), viral hepatitis (30%), biliary disease (5-10%), primary hemochromatosis (5%), and drug-related and cryptogenic cirrhosis of, unknown etiology, (10-15%). Wilson's disease, α₁-antitrypsin deficiency and other rare diseases also have liver fibrosis as one of the symptoms. Liver cirrhosis, the end stage of liver fibrosis, frequently requires liver transplantation and is among the top ten causes of death in the Western world.

Kidney Fibrosis and Related Conditions

Chronic Renal Failure (CRF)

Chronic renal failure is a gradual and progressive loss of the ability of the kidneys to excrete wastes, concentrate urine, and conserve electrolytes. CRF is slowly progressive. It most often results from any disease that causes gradual loss of kidney function, and fibrosis is the main pathology that produces CRF.

Diabetic Nephropathy

Diabetic nephropathy, hallmarks of which are glomerulosclerosis and tubulointerstitial fibrosis, is the single most prevalent cause of end-stage renal disease in the modern world, and diabetic patients constitute the largest population on dialysis. Such therapy is costly and far from optimal. Transplantation offers a better outcome but suffers from a severe shortage of donors.

Chronic Kidney Disease

Chronic kidney disease (CKD) is a worldwide public health problem and is recognized as a common condition that is associated with an increased risk of cardiovascular disease and chronic renal failure (CRF).

The Kidney Disease Outcomes Quality Initiative (K/DOQI) of the National Kidney Foundation (NKF) defines chronic kidney disease as either kidney damage or a decreased kidney glomerular filtration rate (GFR) for three or more months. Other markers of CKD are also known and used for diagnosis. In general, the destruction of renal mass with irreversible sclerosis and loss of nephrons leads to a progressive decline in GFR. Recently, the K/DOQI published a classification of the stages of CKD, as follows:

Stage 1: Kidney damage with normal or increased GFR (>90 mL/min/1.73 m2)

Stage 2: Mild reduction in GFR (60-89 mL/min/1.73 m2)

Stage 3: Moderate reduction in GFR (30-59 mL/min/1.73 m2)

Stage 4: Severe reduction in GFR (15-29 mL/min/1.73 m2)

Stage 5: Kidney failure (GFR<15 mL/min/1.73 m2 or dialysis)

In stages 1 and 2 CKD, GFR alone does not confirm the diagnosis. Other markers of kidney damage, including abnormalities in the composition of blood or urine or abnormalities in imaging tests, may be relied upon.

Pathophysiology of CKD

Approximately 1 million nephrons are present in each kidney, each contributing to the total GFR. Irrespective of the etiology of renal injury, with progressive destruction of nephrons, the kidney is able to maintain GFR by hyperfiltration and compensatory hypertrophy of the remaining healthy nephrons. This nephron adaptability allows for continued normal clearance of plasma solutes so that substances such as urea and creatinine start to show significant increases in plasma levels only after total GFR has decreased to 50%, when the renal reserve has been exhausted. The plasma creatinine value will approximately double with a 50% reduction in GFR. Therefore, a doubling in plasma creatinine from a baseline value of 0.6 mg/dL to 1.2 mg/dL in a patient actually represents a loss of 50% of functioning nephron mass.

The residual nephron hyperfiltration and hypertrophy, although beneficial for the reasons noted, is thought to represent a major cause of progressive renal dysfunction. This is believed to occur because of increased glomerular capillary pressure, which damages the capillaries and leads initially to focal and segmental glomerulosclerosis and eventually to global glomerulosclerosis. This hypothesis has been based on studies of five-sixths nephrectomized rats, which develop lesions that are identical to those observed in humans with CKD.

The two most common causes of chronic kidney disease are diabetes and hypertension. Other factors include acute insults from nephrotoxins, including contrasting agents, or decreased perfusion; proteinuria; increased renal ammoniagenesis with interstitial injury; hyperlipidemia; Hyperphosphatemia with calcium phosphate deposition; decreased levels of nitrous oxide and smoking.

In the United States, the incidence and prevalence of CKD is rising, with poor outcomes and high cost to the health system. Kidney disease is the ninth leading cause of death in the US. The high rate of mortality has led the US Surgeon General's mandate for America's citizenry, Healthy People 2010, to contain a chapter focused on CKD. The objectives of this chapter are to articulate goals and to provide strategies to reduce the incidence, morbidity, mortality, and health costs of chronic kidney disease in the United States.

The incidence rates of end-stage renal disease (ESRD) have also increased steadily internationally since 1989. The United States has the highest incident rate of ESRD, followed by Japan. Japan has the highest prevalence per million population followed by the United States.

The mortality rates associated with hemodialysis are striking and indicate that the life expectancy of patients entering into hemodialysis is markedly shortened. At every age, patients with ESRD on dialysis have significantly increased mortality when compared with nondialysis patients and individuals without kidney disease. At age 60 years, a healthy person can expect to live for more than 20 years, whereas the life expectancy of a 60-year-old patient starting hemodialysis is closer to 4 years.

Pulmonary Fibrosis

Interstitial pulmonary fibrosis (IPF) is scarring of the lung caused by a variety of inhaled agents including mineral particles, organic dusts, and oxidant gases, or by unknown reasons (idiopathic lung fibrosis). The disease afflicts millions of individuals worldwide, and there are no effective therapeutic approaches. A major reason for the lack of useful treatments is that few of the molecular mechanisms of disease have been defined sufficiently to design appropriate targets for therapy (Lasky, et al., 2000, Environ Health Perspect; 108:751-62).

Cardiac Fibrosis

Heart failure is unique among the major cardiovascular disorders in that it alone is increasing in prevalence while there has been a striking decrease in other conditions. Some of this can be attributed to the aging of the populations of the United States and Europe. The ability to salvage patients with myocardial damage is also a major factor, as these patients may develop progression of left ventricular dysfunction due to deleterious remodelling of the heart.

The normal myocardium is composed of a variety of cells, cardiac myocytes and noncardiomyocytes, which include endothelial and vascular smooth muscle cells and fibroblasts.

Structural remodeling of the ventricular wall is a key determinant of clinical outcome in heart disease. Such remodeling involves the production and destruction of extracellular matrix proteins, cell proliferation and migration, and apoptotic and necrotic cell death. Cardiac fibroblasts are crucially involved in these processes, producing growth factors and cytokines that act as autocrine and paracrine factors, as well as extracellular matrix proteins and proteinases. Recent studies have shown that the interactions between cardiac fibroblasts and cardiomyocytes are essential for the progression of cardiac remodeling of which the net effect is deterioration in cardiac function and the onset of heart failure (Manabe, et al., 2002, Circ Res. 13:1103-13).

Burns and Scars

A particular problem which may arise, particularly in fibrotic disease, is contraction of tissues, for example contraction of scars. Contraction of tissues including extracellular matrix components, especially of collagen-including tissues, may occur in connection with many different pathological conditions and with surgical or cosmetic procedures. Contracture, for example, of scars, may cause physical problems, which may lead to the need for medical treatment, or it may cause problems of a purely cosmetic nature. Collagen is the major component of scar and other contracted tissue and as such is the most important structural component to consider. Nevertheless, scar and other contracted tissue also include other structural components, especially other extracellular matrix components, for example, elastin, which may also contribute to contraction of the tissue.

Contraction of collagen-including tissue, which may also include other extracellular matrix components, frequently occurs in the healing of burns. The burns may be chemical, thermal or radiation burns and may be of the eye, the surface of the skin or the skin and the underlying tissues. It may also be the case that there are burns on internal tissues, for example, caused by radiation treatment. Contraction of burnt tissues is often a problem and may lead to physical and/or cosmetic problems, for example, loss of movement and/or disfigurement.

Skin grafts may be applied for a variety of reasons and may often undergo contraction after application. As with the healing of burnt tissues the contraction may lead to both physical and cosmetic problems. It is a particularly serious problem where many skin grafts are needed as, for example, in a serious burns case.

Contraction is also a problem in production of artificial skin. To make a true artificial skin it is necessary to have an epidermis made of epithelial cells (keratinocytes) and a dermis made of collagen populated with fibroblasts. It is important to have both types of cells because they signal and stimulate each other using growth factors. The collagen component of the artificial skin often contracts to less than one tenth of its original area when populated by fibroblasts.

Cicatricial contraction, contraction due to shrinkage of the fibrous tissue of a scar, is common. In some cases the scar may become a vicious cicatrix, a scar in which the contraction causes serious deformity. A patient's stomach may be effectively separated into two separate chambers in an hour-glass contracture by the contraction of scar tissue formed when a stomach ulcer heals. Obstruction of passages and ducts, cicatricial stenosis, may occur due to the contraction of scar tissue. Contraction of blood vessels may be due to primary obstruction or surgical trauma, for example, after surgery or angioplasty. Stenosis of other hollow visci, for examples, ureters, may also occur. Problems may occur where any form of scarring takes place, whether resulting from accidental wounds or from surgery. Conditions of the skin and tendons which involve contraction of collagen-including tissues include post-trauma conditions resulting from surgery or accidents, for example, hand or foot tendon injuries, post-graft conditions and pathological conditions, such as scleroderma, Dupuytren's contracture and epidermolysis bullosa. Scarring and contraction of tissues in the eye may occur in various conditions, for example, the sequelae of retinal detachment or diabetic eye disease (as mentioned above). Contraction of the sockets found in the skull for the eyeballs and associated structures, including extra-ocular muscles and eyelids, may occur if there is trauma or inflammatory damage. The tissues contract within the sockets causing a variety of problems including double vision and an unsightly appearance.

For further information on different types of fibrosis see: Molina V, et al., 2002, Harefuah, 141: 973-8, 1009; Yu, et al., 2002, Curr Opin Pharmacol. 2(2):177-81; Keane, et al., 2003, Am J Kidney Dis. 41: S22-5; Bohle, et al., 1989, Pathol Res Pract. 185:421-40; Kikkawa, et al., 1997, Kidney Int Suppl. 62:S39-40; Bataller et al., 2001, Semin Liver Dis. 21:437-51; Gross, et al., 2001 N Engl J Med. 345:517-25; Frohlich, 2001, Am J Hypertens; 14:194S-199S; Friedman, 2003, J Hepatol. 38:S38-53; Albanis, et al., 2003, Curr Gastroenterol Rep. 5:48-56; Weber, 2000, Curr Opin Cardiol. 15:264-72).

Delivery of Nucleic Acid Molecules and Pharmaceutical Formulations

The retinoid or retinoid conjugate useful for delivery of nucleic acid is in a state in which it is dissolved in or mixed with a medium that can dissolve or retain it.

Any retinoid or retinoid conjugate may be used in the present description as long as it is actively accumulated by stellate cells; examples of retinoid include, but are not limited to, tretinoin, adapalene, retinol palmitate, and in particular vitamin A, saturated vitamin A, retinoic acid, and retinal. Examples of the retinoid-conjugate include PEG-retinoid conjugates. The present description utilizes the property of stellate cells to positively incorporate a retinoid and/or a retinoid conjugate, and by using the retinoid and/or retinoid conjugate as a drug carrier or by bonding to or being included in another drug carrier component, a desired material or body is transported specifically to stellate cells. A retinoid is a member of the class of compounds having a skeleton in which four isoprenoid units are bonded in a head-to-tail manner. See G. P. Moss, “Biochemical Nomenclature and Related Documents,” 2nd Ed. Portland Press, pp. 247-251 (1992). Vitamin A is a generic descriptor for a retinoid qualitatively showing the biological activity of retinol. The retinoid in the present description promotes specific substance delivery to a cancer cell and a CAF (that is, the substance is targeted at these cells). Such a retinoid is not particularly limited, and examples thereof include retinol, Vitamin A, saturated Vitamin A, retinal, retinoic acid, an ester of retinol and a fatty acid, an ester of an aliphatic alcohol and retinoic acid, etretinate, tretinoin, isotretinoin, adapalene, acitretine, tazarotene, and retinol palmitate, and vitamin A analogues such as fenretinide, and bexarotene. Retinoid-conjugates include PEG-conjugates, e.g., diVA-PEG-diVA, shown in the following structure.

The drug carrier of the present description therefore may contain a drug carrier component other than a retinoid and/or retinoid-conjugate. Such a component is not particularly limited, and any component known in the fields of medicine and pharmacy may be used, but it is preferable for it to be capable of including a retinoid and/or retinoid conjugate. Examples of such a component include a lipid, for example, a phospholipid such as glycerophospholipid, a sphingolipid such as sphingomyelin, a sterol such as cholesterol, a vegetable oil such as soybean oil or poppy seed oil, mineral oil, and a lecithin such as egg-yolk lecithin, but the examples are not limited thereto. Among them, those that can form a liposome are preferable, for example, natural phospholipids such as lecithin, semisynthetic phospholipids such as dimyristoylphosphatidylcholine (DMPC), dipalmitoylphosphatidylcholine (DPPC), and distearoylphosphatidylcholine (DSPC), and cholesterol.

Furthermore, the drug carrier of the present description may contain a substance that improves incorporation into stellate cells, for example, retinol-binding protein (RBP).

The bonding or inclusion of the retinoid and/or retinoid conjugate with the drug carrier of the present description may also be carried out by bonding or including the retinoid and/or retinoid conjugate with another component of the drug carrier by chemical and/or physical methods. Alternatively, bonding or inclusion of the retinoid and/or retinoid conjugate with the drug carrier of the present description may also be carried out by mixing the retinoid and/or retinoid conjugate having formation-affinity and basic components of the drug carrier, into the drug carrier components during preparation of the drug carrier. The amount of retinoid and/or retinoid conjugate bonded to or included in the drug carrier of the present description may be 0.01% to 100% as a ratio by weight relative to the drug carrier components, preferably 0.2% to 20%, and more preferably 1% to 5%.

Nucleic acid delivery systems may include, for example, aqueous and nonaqueous gels, creams, multiple emulsions, microemulsions, liposomes, ointments, aqueous and nonaqueous solutions, lotions, aerosols, hydrocarbon bases and powders, and can contain excipients such as solubilizers, permeation enhancers (e.g., fatty acids, fatty acid esters, fatty alcohols and amino acids), and hydrophilic polymers (e.g., polycarbophil and polyvinylpyrolidone). In one embodiment, the pharmaceutically acceptable carrier is a liposome or a transdermal enhancer. Examples of liposomes which can be used in this description include the following:

-   -   CellFectin, a 1:1.5 (M/M) liposome formulation of the cationic         lipid         N,N^(I),N^(II),N^(III)-tetramethyl-N,N^(I),N^(II),N^(III)-tetrapalmityl-spermine         and dioleoyl phosphatidylethanolamine (DOPE) (GIBCO BRL);     -   Cytofectin GSV, a 2:1 (M/M) liposome formulation of a cationic         lipid and DOPE (Glen Research);     -   DOTAP         (N-[1-(2,3-dioleoyloxy)-N,N,N-tri-methyl-ammoniummethylsulfate)         (Boehringer Manheim);     -   Lipofectamine, a 3:1 (M/M) liposome formulation of the         polycationic lipid DOSPA, the neutral lipid DOPE (GIBCO BRL) and         Di-Alkylated Amino Acid (DiLA2);     -   Lipotrust, a 4:3:3 (M/M) liposome formulation of         O,O′-ditetradecanoyl-N-(α-trimethylammonioacetyl) diethanolamine         chloride (DC-6-14, cholesterol and         dioleoylphosphatidylethanolamine (Hokkaido System Science).         DC-6-14 consists of the following structure.

Other lipids may be useful: permanent cationic lipids and ionizable cationic lipids, including

and PEG-lipids, including

-   -   1,2-dimyristoleoyl-sn-glycero-3-phosphoethanolamine-N-PEG         (PEG-DMPE)     -   1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-PEG         (PEG-DPPE),     -   1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-PEG         (PEG-DSPE), or     -   1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-PEG (PEG-DOPE)         and/or     -   PEG-ceramide.

Delivery systems may include patches, tablets, suppositories, pessaries, gels and creams, and can contain excipients such as solubilizers and enhancers (e.g., propylene glycol, bile salts and amino acids), and other vehicles (e.g., polyethylene glycol, fatty acid esters and derivatives, and hydrophilic polymers such as hydroxypropylmethylcellulose and hyaluronic acid).

The drug carrier of the present description may be in any form as long as a desired material or body can be transported to target stellate cells, and examples of the form include, but are not limited to, polymer micelle, liposome, emulsion, microsphere, and nanosphere. Furthermore, the drug carrier of the present description may include in its interior the substance that is to be transported, be attached to the exterior of the substance that is to be transported, or be mixed with the substance that is to be transported as long as the retinoid and/or retinoid conjugate included therein is at least partially exposed on the exterior of the preparation before it reaches the stellate cells at the latest.

The drug carrier of the present description specifically targets stellate cells and enables a desired effect such as, for example, inhibition or prevention of fibrosis to be exhibited with the maximum effect and minimum side effects by efficiently transporting to stellate cells a desired material or body such as, for example, a drug for controlling the activity or growth of stellate cells. The material or body that the present drug carrier delivers is not particularly limited, but it preferably has a size that enables physical movement in a living body from an administration site to the liver, pancreas, etc., where stellate cells are present. The drug carrier of the present description therefore can transport not only a material such as an atom, a molecule, a compound, a protein, or a nucleic acid but also a body such as a vector, a virus particle, a cell, a drug release system constituted from one or more elements, or a micromachine. The material or body preferably has the property of exerting some effect on stellate cells, and examples thereof include one that labels stellate cells and one that controls the activity or growth of stellate cells.

Therefore, in one embodiment of the present description, it is a drug for controlling the activity or growth of stellate cells that the drug carrier delivers. This may be any drug that directly or indirectly inhibits the physicochemical actions of stellate cells involved in the promotion of fibrosis, and examples thereof include, but are not limited to, TGFβ activity inhibitors such as a truncated TGFβ type II receptor and a soluble TGFβ type II receptor, growth factor preparations such as HGF and expression vectors therefor, MMP production promoters such as an MMP gene-containing adenovirus vector, TIMP production inhibitors such as an antisense TIMP nucleic acid, a PPARγ ligand, cell activation inhibitors and/or cell growth inhibitors such as an angiotensin activity inhibitor, a PDGF activity inhibitor, and a sodium channel inhibitor, and also apoptosis inducers such as compound 861 and gliotoxin, adiponectin, and a compound having Rho kinase inhibitory activity such as (+)-trans-4-(1-aminoethyl)-1-(4-pyridylcarbamoyl)cyclohexane. Furthermore, the ‘drug for controlling the activity or growth of stellate cells’ in the present description may be any drug that directly or indirectly promotes the physicochemical actions of stellate cells directly or indirectly involved in the inhibition of fibrosis, and examples thereof include, but are not limited to, a drug for promoting a collagen degradation system, e.g., MMP production promoters such as an MMP expression vector, HGF, and drugs having HGF-like activity such as HGF analogues and expression vectors therefor.

Other examples of the drug for controlling the activity or growth of stellate cells in the present description include a drug for controlling the metabolism of an extracellular matrix such as collagen, for example, a substance having an effect in inhibiting the expression of a target molecule, such as siRNA, ribozyme, and antisense nucleic acid (including RNA, DNA, PNA, and a composite thereof), a substance having a dominant negative effect, and vectors expressing same, that target, for example, an extracellular matrix constituent molecule produced by stellate cells or target one or more molecules that have the function of producing or secreting the extracellular matrix constituent molecule.

The present description also relates to a medicine for treating a stellate cell-related disorder, the medicine containing the drug carrier and the drug for controlling the activity or growth of stellate cells, and relates to the use of the drug carrier in the production of a pharmaceutical composition for treating a stellate cell-related disorder. The stellate cell-related disorder referred to here means a disorder in which stellate cells are directly or indirectly involved in the process of the disorder, that is, the onset, exacerbation, improvement, remission, cure, etc. of the disorder, and examples thereof include hepatic disorders such as hepatitis, in particular chronic hepatitis, hepatic fibrosis, hepatic cirrhosis, and liver cancer, and pancreatic disorders such as pancreatitis, in particular chronic pancreatitis, pancreatic fibrosis, and pancreatic cancer.

In the medicine of the present description, the drug carrier may include a drug in its interior, be attached to the exterior of a drug-containing substance, or be mixed with a drug as long as the retinoid and/or retinoid-conjugate included in the drug carrier is at least partially exposed on the exterior of the preparation before it reaches the stellate cells at the latest. Therefore, depending on the route of administration or manner in which the drug is released, the medicine may be covered with an appropriate material, such as, for example, an enteric coating or a material that disintegrates over time, or may be incorporated into an appropriate drug release system.

The present description therefore includes a drug carrier or medicine preparation kit containing one or more containers containing one or more of a drug carrier constituent, a retinoid and/or a retinoid conjugate, and/or a drug, and also includes an essential component for the drug carrier or the medicine provided in the form of such a kit. The kit of the present description may contain, in addition to those described above, a description, etc. in which a preparation method or an administration method for the drug carrier and the medicine of the present description is described. Furthermore, the kit of the present description may contain all components for completing the drug carrier or the medicine of the present description but need not necessarily contain all of the components. The kit of the present description therefore need not contain a reagent or a solvent that is normally available at a place of medical treatment, an experimental facility, etc. such as, for example, sterile water, saline, or a glucose solution.

The present description further relates to a method for treating a stellate cell-related disorder, the method including administering an effective amount of the medicine to a subject in need thereof. The effective amount referred to here is an amount that suppresses onset of the target disorder, reduces symptoms thereof, or prevents progression thereof, and is preferably an amount that prevents onset of the target disorder or cures the target disorder. It is also preferably an amount that does not cause an adverse effect that exceeds the benefit from administration. Such an amount may be determined as appropriate by an in vitro test using cultured cells, etc. or by a test in a model animal such as a mouse, a rat, a dog, or a pig, and such test methods are well known to a person skilled in the art.

In the method of the present description, the term ‘subject’ means any living individual, preferably an animal, more preferably a mammal, and yet more preferably a human individual. In the present description, the subject may be healthy or affected with some disorder, and in the case of treatment of a disorder being intended, the subject typically means a subject affected with the disorder or having a risk of being affected.

Furthermore, the term ‘treatment’ includes all types of medically acceptable prophylactic and/or therapeutic intervention for the purpose of the cure, temporary remission, prevention, etc. of a disorder. For example, when the disorder is hepatic fibrosis, the term ‘treatment’ includes medically acceptable intervention for various purposes including delaying or halting the progression of fibrosis, regression or disappearance of lesions, prevention of the onset of fibrosis, or prevention of recurrence.

The present description also relates to a method for delivering a drug to stellate cells using the drug carrier. This method includes, but is not limited to, a step of supporting a substance to be delivered on the drug carrier, and a step of administering or adding the drug carrier carrying the substance to be delivered to a stellate cell-containing living body or medium, such as, for example, a culture medium. These steps may be achieved as appropriate in accordance with any known method, the method described in the present specification, etc. This delivery method may be combined with another delivery method, for example, another delivery method in which an organ where stellate cells are present is the target, etc.

Nucleic acid molecules may be adapted for use to prevent or treat fibroses (e.g., liver, kidney, peritoneal, and pulmonary) diseases, traits, conditions and/or disorders, and/or any other trait, disease, disorder or condition that is related to or will respond to the levels of hsp47 in a cell or tissue, alone or in combination with other therapies. A nucleic acid molecule may include a delivery vehicle, including liposomes, for administration to a subject, carriers and diluents and their salts, and/or can be present in pharmaceutically acceptable formulations.

The nucleic acid molecules of the description may include sequences shown in Table 3. Examples of such nucleic acid molecules consist essentially of sequences provided in Table 3.

The nucleic acid molecules may be administered via pulmonary delivery, such as by inhalation of an aerosol or spray dried formulation administered by an inhalation device or nebulizer, providing rapid local uptake of the nucleic acid molecules into relevant pulmonary tissues. Solid particulate compositions containing respirable dry particles of micronized nucleic acid compositions can be prepared by grinding dried or lyophilized nucleic acid compositions, and then passing the micronized composition through, for example, a 400 mesh screen to break up or separate out large agglomerates. A solid particulate composition comprising the nucleic acid compositions of the description can optionally contain a dispersant which serves to facilitate the formation of an aerosol as well as other therapeutic compounds. A suitable dispersant is lactose, which can be blended with the nucleic acid compound in any suitable ratio, such as a 1 to 1 ratio by weight.

Aerosols of liquid particles may include a nucleic acid molecules disclosed herein and can be produced by any suitable means, such as with a nebulizer (see e.g., U.S. Pat. No. 4,501,729). Nebulizers are commercially available devices which transform solutions or suspensions of an active ingredient into a therapeutic aerosol mist either by means of acceleration of a compressed gas, typically air or oxygen, through a narrow venturi orifice or by means of ultrasonic agitation. Suitable formulations for use in nebulizers include the active ingredient in a liquid carrier in an amount of up to 40% w/w preferably less than 20% w/w of the formulation. The carrier is typically water or a dilute aqueous alcoholic solution, preferably made isotonic with body fluids by the addition of, e.g., sodium chloride or other suitable salts. Optional additives include preservatives if the formulation is not prepared sterile, e.g., methyl hydroxybenzoate, anti-oxidants, flavorings, volatile oils, buffering agents and emulsifiers and other formulation surfactants. The aerosols of solid particles including the active composition and surfactant can likewise be produced with any solid particulate aerosol generator. Aerosol generators for administering solid particulate therapeutics to a subject produce particles which are respirable, as explained above, and generate a volume of aerosol containing a predetermined metered dose of a therapeutic composition at a rate suitable for human administration. One illustrative type of solid particulate aerosol generator is an insufflator. Suitable formulations for administration by insufflation include finely comminuted powders which can be delivered by means of an insufflator. In the insufflator, the powder, e.g., a metered dose thereof effective to carry out the treatments described herein, is contained in capsules or cartridges, typically made of gelatin or plastic, which are either pierced or opened in situ and the powder delivered by air drawn through the device upon inhalation or by means of a manually-operated pump. The powder employed in the insufflator consists either solely of the active ingredient or of a powder blend comprising the active ingredient, a suitable powder diluent, such as lactose, and an optional surfactant. The active ingredient typically includes from 0.1 to 100 w/w of the formulation. A second type of illustrative aerosol generator includes a metered dose inhaler. Metered dose inhalers are pressurized aerosol dispensers, typically containing a suspension or solution formulation of the active ingredient in a liquefied propellant. During use these devices discharge the formulation through a valve adapted to deliver a metered volume to produce a fine particle spray containing the active ingredient. Suitable propellants include certain chlorofluorocarbon compounds, e.g., dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane and mixtures thereof. The formulation can additionally contain one or more co-solvents, for example, ethanol, emulsifiers and other formulation surfactants, such as oleic acid or sorbitan trioleate, anti-oxidants and suitable flavoring agents. Other methods for pulmonary delivery are described in, e.g., US20040037780, U.S. Pat. No. 6,592,904, U.S. Pat. No. 6,582,728, and U.S. Pat. No. 6,565,885. WO08132723 relates to aerosol delivery of oligonucleotides in general, and of siRNA in particular, to the respiratory system.

Nucleic acid molecules may be administered to the central nervous system (CNS) or peripheral nervous system (PNS). Experiments have demonstrated the efficient in vivo uptake of nucleic acids by neurons. See e.g., Sommer et al., 1998, Antisense Nuc. Acid Drug Dev., 8:75; Epa et al., 2000, Antisense Nuc. Acid Drug Dev., 10:469; Broaddus et al., 1998, J. Neurosurg., 88:734; Karle et al., 1997, Eur. J. Pharmocol., 340:153; Bannai et al., 1998, Brain Research, 784:304; Rajakumar et al., 1997, Synapse, 26:199; Wu-pong et al., 1999, BioPharm, 12:32; Bannai et al., 1998, Brain Res. Protoc., 3:83; and Simantov et al., 1996, Neuroscience, 74:39. Nucleic acid molecules are therefore amenable to delivery to and uptake by cells in the CNS and/or PNS.

Delivery of nucleic acid molecules to the CNS is provided by a variety of different strategies. Traditional approaches to CNS delivery that can be used include, but are not limited to, intrathecal and intracerebroventricular administration, implantation of catheters and pumps, direct injection or perfusion at the site of injury or lesion, injection into the brain arterial system, or by chemical or osmotic opening of the blood-brain barrier. Other approaches can include the use of various transport and carrier systems, for example though the use of conjugates and biodegradable polymers. Furthermore, gene therapy approaches, e.g., as described in Kaplitt et al., U.S. Pat. No. 6,180,613 and Davidson, WO 04/013280, can be used to express nucleic acid molecules in the CNS.

Nucleic acid molecules may be formulated or complexed with polyethylenimine (e.g., linear or branched PEI) and/or polyethylenimine derivatives, including for example grafted PEIs such as galactose PEI, cholesterol PEI, antibody derivatized PEI, and polyethylene glycol PEI (PEG-PEI) derivatives thereof (see for example Ogris et al., 2001, AAPA PharmSci, 3, 1-11; Furgeson et al., 2003, Bioconjugate Chem., 14, 840-847; Kunath et al., 2002, Pharm Res 19:810-17; Choi et al., 2001, Bull. Korean Chem. Soc., 22:46-52; Bettinger et al., 1999, Bioconjugate Chem., 10:558-561; Peterson et al., 2002, Bioconjugate Chem. 13:845-54; Erbacher et aL, 1999, J Gene Med 1:1-18; Godbey et al., 1999, PNAS, 96:5177-81; Godbey et al., 1999, J Controlled Release, 60:149-60; Diebold et al., 1999, J Biol Chem, 274:19087-94; Thomas et al., 2002, PNAS, 99, 14640-45; and Sagara, U.S. Pat. No. 6,586,524).

Nucleic acid molecules may include a bioconjugate, for example a nucleic acid conjugate as described in Vargeese et al., U.S. Ser. No. 10/427,160; U.S. Pat. No. 6,528,631; U.S. Pat. No. 6,335,434; U.S. Pat. No. 6,235,886; U.S. Pat. No. 6,153,737; U.S. Pat. No. 5,214,136; U.S. Pat. No. 5,138,045.

Compositions, methods and kits disclosed herein may include an expression vector that includes a nucleic acid sequence encoding at least one nucleic acid molecule of the description in a manner that allows expression of the nucleic acid molecule. Methods of introducing nucleic acid molecules or one or more vectors capable of expressing the strands of dsRNA into the environment of the cell will depend on the type of cell and the make up of its environment. The nucleic acid molecule or the vector construct may be directly introduced into the cell (i.e., intracellularly); or introduced extracellularly into a cavity, interstitial space, into the circulation of an organism, introduced orally, or may be introduced by bathing an organism or a cell in a solution containing dsRNA. The cell is preferably a mammalian cell; more preferably a human cell. The nucleic acid molecule of the expression vector can include a sense region and an antisense region. The antisense region can include a sequence complementary to a RNA or DNA sequence encoding hsp47 and the sense region can include a sequence complementary to the antisense region. The nucleic acid molecule can include two distinct strands having complementary sense and antisense regions. The nucleic acid molecule can include a single-strand having complementary sense and antisense regions.

Nucleic acid molecules that interact with target RNA molecules and down-regulate gene encoding target RNA molecules (e.g., target RNA molecules referred to by Genbank Accession numbers herein) may be expressed from transcription units inserted into DNA or RNA vectors. Recombinant vectors can be DNA plasmids or viral vectors. Nucleic acid molecule expressing viral vectors can be constructed based on, but not limited to, adeno-associated virus, retrovirus, adenovirus, or alphavirus. The recombinant vectors capable of expressing the nucleic acid molecules can be delivered as described herein, and persist in target cells. Alternatively, viral vectors can be used that provide for transient expression of nucleic acid molecules. Such vectors can be repeatedly administered as necessary. Once expressed, the nucleic acid molecules bind and down-regulate gene function or expression via RNA interference (RNAi). Delivery of nucleic acid molecule expressing vectors can be systemic, such as by intravenous or intramuscular administration, by administration to target cells ex-planted from a subject followed by reintroduction into the subject, or by any other means that would allow for introduction into the desired target cell.

Expression vectors may include a nucleic acid sequence encoding at least one nucleic acid molecule disclosed herein, in a manner which allows expression of the nucleic acid molecule. For example, the vector may contain sequence(s) encoding both strands of a nucleic acid molecule that include a duplex. The vector can also contain sequence(s) encoding a single nucleic acid molecule that is self-complementary and thus forms a nucleic acid molecule. Non-limiting examples of such expression vectors are described in Paul et al., 2002, Nature Biotech 19, 505; Miyagishi et al., 2002, Nature Biotech 19, 497; Lee et al., 2002, Nature Biotech 19, 500; and Novina et al., 2002, Nature Med:10.1038/nm725. Expression vectors may also be included in a mammalian (e.g., human) cell.

An expression vector may include a nucleic acid sequence encoding two or more nucleic acid molecules, which can be the same or different. Expression vectors may include a sequence for a nucleic acid molecule complementary to a nucleic acid molecule referred to by a Genbank Accession number NM 001235, for example those shown in Table 2.

An expression vector may encode one or both strands of a nucleic acid duplex, or a single self-complementary strand that self hybridizes into a nucleic acid duplex. The nucleic acid sequences encoding nucleic acid molecules can be operably linked in a manner that allows expression of the nucleic acid molecule (see for example Paul et al., 2002, Nature Biotech, 19:505; Miyagishi and Taira, 2002, Nature Biotech 19:497; Lee et al., 2002, Nature Biotech 19:500; and Novina et al., 2002, Nature Med, 10.1038/nm725).

An expression vector may include one or more of the following: a) a transcription initiation region (e.g., eukaryotic pol I, II or III initiation region); b) a transcription termination region (e.g., eukaryotic pol I, II or III termination region); c) an intron and d) a nucleic acid sequence encoding at least one of the nucleic acid molecules, wherein said sequence is operably linked to the initiation region and the termination region in a manner that allows expression and/or delivery of the nucleic acid molecule. The vector can optionally include an open reading frame (ORF) for a protein operably linked on the 5′ side or the 3′-side of the sequence encoding the nucleic acid molecule; and/or an intron (intervening sequences).

Transcription of the nucleic acid molecule sequences can be driven from a promoter for eukaryotic RNA polymerase I (pol I), RNA polymerase II (pol II), or RNA polymerase III (pol III). Transcripts from pol II or pol III promoters are expressed at high levels in all cells; the levels of a given pol II promoter in a given cell type depends on the nature of the gene regulatory sequences (enhancers, silencers, etc.) present nearby. Prokaryotic RNA polymerase promoters are also used, providing that the prokaryotic RNA polymerase enzyme is expressed in the appropriate cells (Elroy et al., 1990, PNAS, 87:6743-47; Gao et al., 1993, Nucleic Acids Res 21:2867-72; Lieber et al., 1993, Methods Enzymol., 217:47-66; Zhou et al., 1990, Mol. Cell. Biol. 10:4529-37). Several investigators have demonstrated that nucleic acid molecules expressed from such promoters can function in mammalian cells (e.g. Kashani-Sabet et al., 1992, Antisense Res. Dev., 2:3-15; Ojwang et al., 1992, PNAS 89:10802-06; Chen et al., 1992, Nucleic Acids Res., 20:4581-89; Yu et al., 1993, PNAS, 90:6340-44; L'Huillier et al., 1992, EMBO J., 11:4411-18; Lisziewicz et al., 1993, PNAS 90: 8000-04; Thompson et al., 1995, Nucleic Acids Res., 23:2259; Sullenger et al., 1993, Science, 262:1566). More specifically, transcription units such as the ones derived from genes encoding U6 small nuclear (snRNA), transfer RNA (tRNA) and adenovirus VA RNA are useful in generating high concentrations of desired RNA molecules such as siNA in cells (Thompson et al., supra; Couture and Stinchcomb, 1996, supra; Noonberg et al., 1994, Nucleic Acid Res. 22:2830; Noonberg et al., U.S. Pat. No. 5,624,803; Good et al., 1997, Gene Ther., 4:45; Beigelman et al., Int'l PCT Publication No. WO 96/18736. The above nucleic acid transcription units can be incorporated into a variety of vectors for introduction into mammalian cells, including but not restricted to, plasmid DNA vectors, viral DNA vectors (such as adenovirus or adeno-associated virus vectors), or viral RNA vectors (such as retroviral or alphavirus vectors) (see Couture and Stinchcomb, 1996 supra).

Nucleic acid molecule may be expressed within cells from eukaryotic promoters (e.g., Izant and Weintraub, 1985, Science, 229, 345; McGarry and Lindquist, 1986, PNAS 83, 399; Scanlon et al., 1991, PNAS 88:10591-95; Kashani-Sabet et al., 1992, Antisense Res. Dev., 2:3-15; Dropulic et al., 1992, J. Virol., 66:1432-41; Weerasinghe et al., 1991, J. Virol., 65:5531-34; Ojwang et al., 1992, PNAS, 89:10802-06; Chen et al., 1992, Nucleic Acids Res., 20:4581-89; Sarver et al., 1990 Science, 247:1222-25; Thompson et al., 1995, Nucleic Acids Res., 23:2259; Good et al., 1997, Gene Therapy, 4:45. Those skilled in the art realize that any nucleic acid can be expressed in eukaryotic cells from the appropriate DNA/RNA vector. The activity of such nucleic acids can be augmented by their release from the primary transcript by a enzymatic nucleic acid (Draper et al., PCT WO 93/23569, and Sullivan et al., PCT WO 94/02595; Ohkawa et al., 1992, Nucleic Acids Symp. Ser., 27:15-6; Taira et al., 1991, Nucleic Acids Res., 19:5125-30; Ventura et al., 1993, Nucleic Acids Res., 21:3249-55; Chowrira et al., 1994, J. Biol. Chem., 269:25856.

A viral construct packaged into a viral particle would accomplish both efficient introduction of an expression construct into the cell and transcription of dsRNA construct encoded by the expression construct.

Methods for oral introduction include direct mixing of RNA with food of the organism, as well as engineered approaches in which a species that is used as food is engineered to express an RNA, then fed to the organism to be affected. Physical methods may be employed to introduce a nucleic acid molecule solution into the cell. Physical methods of introducing nucleic acids include injection of a solution containing the nucleic acid molecule, bombardment by particles covered by the nucleic acid molecule, soaking the cell or organism in a solution of the RNA, or electroporation of cell membranes in the presence of the nucleic acid molecule.

Other methods known in the art for introducing nucleic acids to cells may be used, such as lipid-mediated carrier transport, chemical mediated transport, such as calcium phosphate, and the like. Thus the nucleic acid molecules may be introduced along with components that perform one or more of the following activities: enhance RNA uptake by the cell, promote annealing of the duplex strands, stabilize the annealed strands, or other-wise increase inhibition of the target gene.

Dosages

The useful dosage to be administered and the particular mode of administration will vary depending upon such factors as the cell type, or for in vivo use, the age, weight and the particular animal and region thereof to be treated, the particular nucleic acid and delivery method used, the therapeutic or diagnostic use contemplated, and the form of the formulation, for example, suspension, emulsion, micelle or liposome, as will be readily apparent to those skilled in the art. Typically, dosage is administered at lower levels and increased until the desired effect is achieved.

When lipids are used to deliver the nucleic acid, the amount of lipid compound that is administered can vary and generally depends upon the amount of nucleic acid being administered. For example, the weight ratio of lipid compound to nucleic acid is preferably from about 1:1 to about 30:1, with a weight ratio of about 5:1 to about 15:1 being more preferred.

A suitable dosage unit of nucleic acid molecules may be in the range of 0.001 to 0.25 milligrams per kilogram body weight of the recipient per day, or in the range of 0.01 to 20 micrograms per kilogram body weight per day, or in the range of 0.01 to 10 micrograms per kilogram body weight per day, or in the range of 0.10 to 5 micrograms per kilogram body weight per day, or in the range of 0.1 to 2.5 micrograms per kilogram body weight per day.

Suitable amounts of nucleic acid molecules may be introduced and these amounts can be empirically determined using standard methods. Effective concentrations of individual nucleic acid molecule species in the environment of a cell may be about 1 femtomolar, about 50 femtomolar, 100 femtomolar, 1 picomolar, 1.5 picomolar, 2.5 picomolar, 5 picomolar, 10 picomolar, 25 picomolar, 50 picomolar, 100 picomolar, 500 picomolar, 1 nanomolar, 2.5 nanomolar, 5 nanomolar, 10 nanomolar, 25 nanomolar, 50 nanomolar, 100 nanomolar, 500 nanomolar, 1 micromolar, 2.5 micromolar, 5 micromolar, 10 micromolar, 100 micromolar or more.

Dosage levels of the order of from about 0.1 mg to about 140 mg per kilogram of body weight per day are useful in the treatment of the above-indicated conditions (about 0.5 mg to about 7 g per subject per day). The amount of active ingredient that can be combined with the carrier materials to produce a single dosage form varies depending upon the host treated and the particular mode of administration. Dosage unit forms generally contain between from about 1 mg to about 500 mg of an active ingredient.

It is understood that the specific dose level for any particular subject depends upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of administration, and rate of excretion, drug combination and the severity of the particular disease undergoing therapy.

Pharmaceutical compositions that include the nucleic acid molecule disclosed herein may be administered once daily, qid, tid, bid, QD, or at any interval and for any duration that is medically appropriate. However, the therapeutic agent may also be dosed in dosage units containing two, three, four, five, six or more sub-doses administered at appropriate intervals throughout the day. In that case, the nucleic acid molecules contained in each sub-dose may be correspondingly smaller in order to achieve the total daily dosage unit. The dosage unit can also be compounded for a single dose over several days, e.g., using a conventional sustained release formulation which provides sustained and consistent release of the dsRNA over a several day period. Sustained release formulations are well known in the art. The dosage unit may contain a corresponding multiple of the daily dose. The composition can be compounded in such a way that the sum of the multiple units of a nucleic acid together contain a sufficient dose.

Pharmaceutical Compositions, Kits, and Containers

Also provided are compositions, kits, containers and formulations that include a nucleic acid molecule (e.g., an siNA molecule) as provided herein for reducing expression of hsp47 for administering or distributing the nucleic acid molecule to a patient. A kit may include at least one container and at least one label. Suitable containers include, for example, bottles, vials, syringes, and test tubes. The containers can be formed from a variety of materials such as glass, metal or plastic. The container can hold amino acid sequence(s), small molecule(s), nucleic acid sequence(s), cell population(s) and/or antibody(s). In one embodiment, the container holds a polynucleotide for use in examining the mRNA expression profile of a cell, together with reagents used for this purpose. In another embodiment a container includes an antibody, binding fragment thereof or specific binding protein for use in evaluating hsp47 protein expression cells and tissues, or for relevant laboratory, prognostic, diagnostic, prophylactic and therapeutic purposes; indications and/or directions for such uses can be included on or with such container, as can reagents and other compositions or tools used for these purposes. Kits may further include associated indications and/or directions; reagents and other compositions or tools used for such purpose can also be included.

The container can alternatively hold a composition that is effective for treating, diagnosis, prognosing or prophylaxing a condition and can have a sterile access port (for example the container can be an intravenous solution bag or a vial having a stopper pierceable by a hypodermic injection needle). The active agents in the composition can be a nucleic acid molecule capable of specifically binding hsp47 and/or modulating the function of hsp47.

A kit may further include a second container that includes a pharmaceutically-acceptable buffer, such as phosphate-buffered saline, Ringer's solution and/or dextrose solution. It can further include other materials desirable from a commercial and user standpoint, including other buffers, diluents, filters, stirrers, needles, syringes, and/or package inserts with indications and/or instructions for use.

The units dosage ampoules or multidose containers, in which the nucleic acid molecules are packaged prior to use, may include an hermetically sealed container enclosing an amount of polynucleotide or solution containing a polynucleotide suitable for a pharmaceutically effective dose thereof, or multiples of an effective dose. The polynucleotide is packaged as a sterile formulation, and the hermetically sealed container is designed to preserve sterility of the formulation until use.

The container in which the polynucleotide including a sequence encoding a cellular immune response element or fragment thereof may include a package that is labeled, and the label may bear a notice in the form prescribed by a governmental agency, for example the Food and Drug Administration, which notice is reflective of approval by the agency under Federal law, of the manufacture, use, or sale of the polynucleotide material therein for human administration.

Federal law requires that the use of pharmaceutical compositions in the therapy of humans be approved by an agency of the Federal government. In the United States, enforcement is the responsibility of the Food and Drug Administration, which issues appropriate regulations for securing such approval, detailed in 21 U.S.C. §301-392. Regulation for biologic material, including products made from the tissues of animals is provided under 42 U.S.C. §262. Similar approval is required by most foreign countries. Regulations vary from country to country, but individual procedures are well known to those in the art and the compositions and methods provided herein preferably comply accordingly.

The dosage to be administered depends to a large extent on the condition and size of the subject being treated as well as the frequency of treatment and the route of administration. Regimens for continuing therapy, including dose and frequency may be guided by the initial response and clinical judgment. The parenteral route of injection into the interstitial space of tissues is preferred, although other parenteral routes, such as inhalation of an aerosol formulation, may be required in specific administration, as for example to the mucous membranes of the nose, throat, bronchial tissues or lungs.

As such, provided herein is a pharmaceutical product which may include a polynucleotide including a sequence encoding a cellular immune response element or fragment thereof in solution in a pharmaceutically acceptable injectable carrier and suitable for introduction interstitially into a tissue to cause cells of the tissue to express a cellular immune response element or fragment thereof, a container enclosing the solution, and a notice associated with the container in form prescribed by a governmental agency regulating the manufacture, use, or sale of pharmaceuticals, which notice is reflective of approval by the agency of manufacture, use, or sale of the solution of polynucleotide for human administration.

Indications

The nucleic acid molecules disclosed herein can be used to treat diseases, conditions or disorders associated with hsp47, such as liver fibrosis, cirrhosis, pulmonary fibrosis, kidney fibrosis, peritoneal fibrosis, chronic hepatic damage, and fibrillogenesis and any other disease or conditions that are related to or will respond to the levels of hsp47 in a cell or tissue, alone or in combination with other therapies. As such, compositions, kits and methods disclosed herein may include packaging a nucleic acid molecule disclosed herein that includes a label or package insert. The label may include indications for use of the nucleic acid molecules such as use for treatment or prevention of liver fibrosis, peritoneal fibrosis, kidney fibrosis and pulmonary fibrosis, and any other disease or conditions that are related to or will respond to the levels of hsp47 in a cell or tissue, alone or in combination with other therapies. A label may include an indication for use in reducing expression of hsp47. A “package insert” is used to refer to instructions customarily included in commercial packages of therapeutic products, that contain information about the indications, usage, dosage, administration, contraindications, other therapeutic products to be combined with the packaged product, and/or warnings concerning the use of such therapeutic products, etc.

Those skilled in the art will recognize that other anti-fibrosis treatments, drugs and therapies known in the art can be readily combined with the nucleic acid molecules herein (e.g. siNA molecules) and are hence contemplated herein.

The methods and compositions provided herein will now be described in greater detail by reference to the following non-limiting examples.

Example 1 Preparation of siRNA for gp46

Among optimal sequences for siRNA recognition in targeting a base sequence of HSP47, which is a common molecular chaperone for collagens (types I to IV), Sequences A and B were prepared in accordance with an siRNA oligo design program by iGENE Therapeutics, Inc. Sequence C was prepared by searching on the Internet using the siRNA Target Finder from Ambion, Inc. and selecting 19 base sequences that would become a target for rat gp46 (human HSP47 homologue, GenBank Accession No. M69246). When carrying out the design, care was taken in starting at 75 to 100 bases downstream from the initiation codon, positioning the first AA dimer, and making sure that the GC content was 30% to 70%. In this example, siRNAs having the sequences below were prepared.

(SEQ ID NO: 2730) A: GUUCCACCAUAAGAUGGUAGACAAC  (SEQ ID NO: 2731) B: CCACAAGUUUUAUAUCCAAUCUAGC  (SEQ ID NO: 2732) C: GAAACCUGUAGAGGCCGCA 

Example 2 Inhibition of gp46 Expression by Prepared siRNA

Normal rat kidney cells (NRK cells), which had rat gp46 and were fibroblasts producing collagen, were transfected with 0.1 nM to 50 nM siRNA and cultured for 12 to 48 hours (FIG. 1). The amount of expression of gp46 was checked by the western blot method (FIGS. 2 to 4, upper band corresponding to gp46, lower band corresponding to actin control). All of the siRNAs inhibited the expression of gp46 protein remarkably compared with a vehicle (FIG. 2). In the experiment below, siRNA Sequence A, which showed the strongest effect, was used. Inhibition by siRNA was concentration dependent (FIG. 3); protein expression by gp46 was about 90% inhibited by 50 nM siRNA at 48 hours (FIG. 4).

Example 3 Inhibition of Collagen Synthesis by Prepared siRNA

In order to examine the amount of collagen synthesized, ³H-proline was added to the culture supernatant of rat fibroblasts (NRK cells) under the above-mentioned conditions (siRNA concentration 50 nM, time 48 hours), and after transfection the amount of ³H in secreted protein was examined (FIG. 5). The amount of collagen synthesized was calculated from the ratio of protein secreted in the supernatant to protein degraded by collagenase when culturing gp46siRNA-transfected fibroblasts in the presence of ³H -proline in accordance with Peterkofsky et al., 1971 Biochemistry 10:988-94.

Collagen synthesis ratio=collagenase−sensitive fraction×100(5.4×collagenase−insensitive fraction+collagenase−sensitive fraction)

The collagen synthesis ratio in rat fibroblasts decreased by about 40% compared with a control group (FIG. 6).

Example 4 Specific Transfection of Nucleic Acid into HSC

An emulsion (VA-Lip-GFP) was prepared by mixing GFP expression plasmid and liposome-encapsulated VA formed by mixing 10% Vitamin A (VA) and liposome. Cationic liposomes containing O,O′-ditetradecanolyl-N-(α-trimethylammonioacetyl) diethanolamine chloride (DC-6-14) as a cationic lipid, cholesterol, and dioleoylphosphatidylethanolamine at a molar ratio of 4:3:3 were dissolved in a chloroform-methanol mixture (4:1, v:v), and the solvent was removed by evaporation under vacuum. The lipids were mixed with an aqueous 9% sucrose solution and hydrated at 60° C. and homogenized to uniformity. The dispersion was extruded twice through a polyvinylidenedifluroide membrane filter with a 0.22 μm pore size. The dispersion was aliquotted into glass vials and frozen and then lyophilized. The dried liposomes were reconsitituted with distilled water at a concentration of 1 mM DC-6-14 under vortexing before use. Specifically, 25 mg of Vitamin A was first dissolved in 87 μL of DMSO thus to give a 100 mM stock solution. To prepare VA-coupled liposomes, 200 nmol of VA dissolved in DMSO was mixed with 100 nmole of DC-6-14 by vortexing at room temperature. The VA-siRNA-liposomes were intraportally administered to a rat, hepatic tissue was collected and fixed. The emulsion was prepared by supposing that the amount of plasma for a 200 g rat was about 10 mL, and setting the concentrations of VA and GFP in portal blood at 10 μM. 1 μL of this VA stock solution was mixed with 10 μL of the VA-liposomes and 179 μL of PBS, 10 μg of GFP expression plasmid was further added thereto to give a total of 200 μL, and the mixture was vortexed for three minutes to give VA-Lip-GFP. The abdomen of an SD rat was opened, and the VA-Lip-GFP was slowly injected into a peripheral portal vein. Forty-eight hours after the injection, hepatic tissue was harvested. Since compared with other hepatic cells intermediate filament desmin is specifically expressed in HSC, when fixed hepatic tissue was stained with Alexa Fluor 568-labeled anti-desmin antibody, and a fluorescence double image with GFP was examined, it was confirmed that GFP was expressed within the HSC (FIG. 7). For untreated controls and a group to which the GFP expression plasmid vector alone was administered, expression in rat HSC was not observed, but in a group to which VA-Lip-GFP was administered, expression of GFP was observed specifically in stellate cells.

Example 5 Quantitative Analysis of Nucleic Acid Transfection Rate

In the same manner as in Example 4, except that FITC-labeled gp46siRNA was used instead of the GFP expression plasmid, an emulsion (VA-Lip-gp46siRNA (FITC)) containing VA-encapsulated liposome and FITC-labeled gp46siRNA was prepared. A solution of siRNAgp46 (580 pmole/μl in distilled water) was added to the VA-coupled liposome solution of Example 4 with stirring at room temperature. The ratio of siRNA to DC-6-14 was 1:11.5 (mol/mol) and the siRNA to liposome ratio (wt/wt) was 1:1. Free VA or siRNA was removed by micropartition using VIVASPIN concentrator, 30K MWCO, by three passes. Material trapped on the membrane was reconstituted with PBS and intraportally administered to an SD rat (10 μg as the amount of siRNA/200 μL). Forty-eight hours after administration hepatic tissue was harvested, αSMA (smooth muscle actin), which compared with other hepatic cells is expressed specifically in HSC, was stained with Alexa Fluor 568-labeled anti-αSMA antibody, cell nuclei were stained with DAPI, and a fluorescence image was examined by a confocal laser scanning microscope (LSM). As shown on the left-hand side of FIG. 8, in a group to which VA-Lip-gp46siRNA (FITC) was administered, a large number of cells emitting both green fluorescence due to FITC and red fluorescence due to Alexa Fluor 568 were observed, and when a quantitative analysis was carried out by NIH Image (the number of cells was counted by selecting any 10 fields from a ×1000 fluorescence microscope photograph), the transfection efficiency was 77.6% (average of 10 fields). On the other hand, in a group to which Lip-gp46siRNA (FITC) containing no VA was administered, the transfection efficiency was a low value of 14.0% and, moreover, transfection into cells other than stellate cells was observed at 3.0% (right-hand side of FIG. 8). It has been found from the results above that the transfection efficiency into stellate cells is increased remarkably by including VA.

Example 6 Inhibition of Expression of gp46 by VA-Lip-gp46siRNA

With regard to another section of the tissue harvested in Example 5, gp46 was stained with Alexa Fluor 568-labeled anti-HSP47 antibody and cell nuclei were stained with DAPI, and a fluorescence image was examined by a confocal laser scanning microscope. As shown in FIG. 9, it was observed that in a group to which VA-Lip-gp46siRNA was administered, expression of gp46, which can be observed as a red fluorescence (right-hand side in the figure), was markedly reduced compared with a control group to which was administered VA-Lip-random siRNA containing random siRNA, which was not specific to gp46 (left-hand side in the figure). The expression inhibition rate relative to an average of six fields of the control group was 75%, which was extremely high, when the number of gp46-negative cells was examined by selecting any ten fields from a ×1000 fluorescence microscope photograph using NIH Image in the same manner as in Example 7.

Example 7 Treatment of LC Rat (Intraportal Administration 1)

In accordance with a report by Jezequel et al. (Jezequel et al., 1987, J Hepatol. 5:174-81), an LC model rat was prepared using dimethylnitrosamine (DMN) (FIG. 10). Specifically, a 1 mL/kg dose of 1% DMN (intraperitoneal administration) was administered to a five week-old SD rat (male) three straight days per week. As already reported, an increase in fiber was observed from the 2nd week, and in the 4th week this was accompanied by the findings of marked fibrosis, destruction of hepatic lobule structure, and formation of regenerative nodules being observed (FIG. 11). Then, by the same method as in Examples 4 and 5, an emulsion (VA-Lip-gp46siRNA) was prepared by formulating gp46siRNA as a liposome and mixing with 10% VA, and was administered. Administration of VA-Lip-gp46siRNA was started in the 3rd week, by which time sufficient fibrosis was observed, and evaluation was carried out in the 4th and 5th weeks. Since it was confirmed by Example 2 that the effects were observed for up to 48 hours in vitro, administration was carried out twice a week (FIG. 11). The amount administered was determined in accordance with a report in which siRNA was directly injected (McCaffery et al., 2002, Nature 418: 38-39), and was 40 μg as the total amount of siRNA. From azan staining of the liver after administration of siRNA, in the 4th week there was no apparent difference between a group to which saline had been administered, a group to which siRNA (random) had been administered, and a group to which siRNA (gp46) had been administered, but in the 5th week a decrease in the amount of fiber was observed for the group to which gp46siRNA had been administered (FIG. 12). In order to quantitatively analyze the amount of fiber, an unstained portion was extracted using NIH Image, its area was measured (FIG. 13), and a significant decrease in the area of collagen was observed for the group to which gp46siRNA had been administered (FIG. 14). Furthermore, in order to evaluate the degree of fibrosis using another measure, the amount of hydroxyproline, which is an indicator for fibrosis, was quantitatively measured by a standard method. Specifically, after 20 mg of freeze-dried hepatic tissue was hydrolyzed with HCl for 24 hours, the reaction liquid was centrifuged, and the supernatant was treated with a reagent such as Ehrlich's solution and centrifuged. The supernatant was recovered, and the amount of hydroxyproline in the hepatic tissue was measured by measuring the absorbance at 560 nm (Hepatology 1998, 28:1247-52). As shown in FIG. 15, in the group to which gp46siRNA had been administered, the amount of hydroxyproline became very small.

Example 8 Treatment of LC Rat (Intraportal Administration 2)

Furthermore, in order to examine a change in the survival rate by administration of the medicine of the present description, in accordance with a method by Qi Z et al. (PNAS 1999; 96:2345-49), an LC model rat was prepared using DMN in an amount that was increased by 20% over the normal amount. In this model, a total of four intraportal administrations were carried out in the first and second weeks. Administration details were: PBS, Lip-gp46siRNA, VA-Lip-random siRNA, and VA-Lip-gp46siRNA (n=7 for each group). After the third week, all of the controls (the group to which PBS had been administered, the group to which VA-Lip-random siRNA had been administered, and the group to which Lip-gp46siRNA had been administered) were dead, but 6 out of 7 survived for the group to which VA-Lip-gp46siRNA had been administered (FIG. 16). Furthermore, in azan staining of the liver on the 21st day, an apparent decrease in the amount of fiber was observed for the group to which gp46siRNA had been administered (FIG. 17).

Example 9 Treatment of LC Rat (Intraportal Administration 3)

In another experiment, intraportal administration was carried out from the 3rd week for LC model rats (1% DMN 1 mg/kg intraperitoneally administered 3 times a week) prepared in accordance with the method by Qi et al. and a method by Ueki et al., 1999, Nat Med. 5:226-30, as shown in Table 2 below (n=6 for each group). PBS was added to each substance to be administered so as to make a total volume of 200 μL, and the frequency of administration was once a week.

TABLE 2 Treatment Content of Frequency of group Administration Dosage Administration 10-1 VA VA 200 nmol Twice a 10-2 Lip-gp46siRNA liposome 100 nmol; week 10-2 gp46siRNA 100 μg 10-3 VA-Lip-random VA 200 nmol; siRNA siRNA liposome 100 nmol; random- 100 μg 10-4 VA-Lip- VA 200 nmol; gp46siRNA liposome 100 nmol; gp46siRNA 100 μg 10-5 PBS 200 μL Three times 10-6 VA VA 200 nmol a week 10-7 VA-Lip VA 200 nmol; liposome 100 nmol 10-8 Lip-gp46siRNA liposome 100 nmol; gp46siRNA 150 μg 10-9 VA-Lip-random VA 200 nmol; siRNA liposome 100 nmol; random-siRNA 150 μg 10-10 VA-Lip- VA 200 nmol; gp46siRNA liposome 100 nmol; gp46siRNA 150 μg

From the results, in the groups other than the group to which the medicine of the present description had been administered (treatment group 9-4), all 6 rats were dead by the 45th day after starting administration of DMN, but in the group to which the medicine of the present description had been administered, all of the individuals apart from one case, which was dead on the 36th day, survived for more than 70 days after starting administration of DMN (FIG. 18). For the dead individuals, the amount of hepatic fiber was quantitatively analyzed based on the area of collagen in the same manner as in Example 7, and the increase in the amount of hepatic fiber was remarkably inhibited by administration of VA-Lip-gp46siRNA (FIG. 19).

Example 10 Treatment of LC Rat (Intravenous Administration)

Intravenous administration was carried out from the 3rd week for LC model rats (1% DMN 1 μg/BW (g) intraperitoneally administered 3 times a week) prepared in the same manner as in Example 9, as shown in the table below (n=6 for each group). PBS was added to each substance to be administered so as to make a total volume of 200 μL. The administration period was up to death except that it was up to the 7th week for Group 10-4 and the 6th week for Group 10-10.

From the results, in the groups other than the groups to which the medicine of the present description had been administered (treatment groups 10-4 and 10-10), all 6 rats were dead by the 45th day after starting administration of DMN, but in the groups to which the medicine of the present description had been administered, all of the individuals, apart from a case in which two rats were dead on the 45th day in treatment group 10-4, survived for more than 70 days after starting administration of DMN (FIGS. 20 and 21). For the dead individuals, the amount of hepatic fiber was quantitatively analyzed in the same manner as in Example 7, and the increase in the amount of hepatic fiber was remarkably inhibited by administration of VA-Lip-gp46siRNA (FIG. 22).

The above-mentioned results show that the medicine of the present description is extremely effective for the prevention and treatment of fibrosis, in which stellate cells are involved.

Example 11 Improvement of Results by RBP (Retinol-Binding Protein)

The influence of RBP on VA-Lip-gp46siRNA transfection efficiency was examined using LI90, a cell line derived from human HSC. 100 nM of VA-Lip-gp46siRNA (FITC) prepared in Example 5, together with various concentrations (i.e. 0, 0.1, 0.5, 1, 2, 4, or 10%) of FBS (fetal bovine serum), were added to LI90 during culturing and incubated for 48 hours, a fluorescence image was observed by LSM, and the amount of siRNA incorporated into individual cells was quantitatively analyzed by FACS. FBS contained about 0.7 mg/dL of RBP. As shown in FIG. 23, FBS (RBP) gave a concentration-dependent increase in the amount of siRNA transfection. Subsequently, 100 nM of VA-Lip-gp46siRNA (FITC) and 4% FBS, together with 10 μg (21.476 nmol) of anti-RBP antibody, were added to LI90 during culturing, and the siRNA transfection efficiency was evaluated in the same manner. As shown in FIG. 24, the increase in the amount of transfection by RBP was markedly decreased by the addition of anti-RBP antibody. The above-mentioned results show that RBP is effective in further enhancing transfection of the medicine of the present description.

Example 12 Selecting hsp47 Nucleic Acid Molecule Sequences

Nucleic acid molecules (e.g., siNA≦25 nucleotides) against Hsp47 were designed using several computer programs including siRNA at Whitehead Institute for Biomedical Research, siRNA Design (Integrated DNA Technologies), BLOCK-iT RNAi Designer (Invitrogen), siDESIGN Center (Dharmacon), and BIOPREDsi (Friedrich Miescher Institute for Biomedical Research). The sequences of top scored siRNAs from these programs were compared and selected (see Table 3) based on the algorithms as well as the sequence homology between human and rat. Candidate sequences were validated by in vitro knocking down assays.

Several parameters were considered for selecting a nucleic acid molecule (e.g., a 21-mer siRNA) sequence. Exemplary parameters include:

-   -   thermodynamic stability (RISC favors the strand with less stable         5′-end)     -   30-52% GC content     -   positional nucleotide preference:

(SEQ ID NO: 2733) (C/G)₁NNNNNNNN(A/U)₁₀(A/U)₁₉

where N is any nucleotide

-   -   devoid of putative immunostimulatory motifs     -   2-nucleotide 3′ overhang     -   position of siRNA within the transcript (preferably within cDNA         region)     -   sequence specificity (checked by using BLAST)     -   variations in single nucleotide by checking SNP database

iRNA sequences having ≦25 nucleotides were designed based on the foregoing methods. Corresponding Dicer substrate siRNA (e.g., ≧26 nucleotides) were designed based on the smaller sequences and extend the target site of the siNA≦25 nucleotide by adding four bases to the 3′-end of the sense strand and 6 bases to the 5′-end of the antisense strand. The Dicer substrates that were made generally have a 25 base sense strand a 27 base antisense strand with an asymmetric blunt ended and 3′-overhang molecule. The sequences of the sense and the antisense strand without base modification (base sequence) and with modifications (experimental sequence) are provided in Table 3.

TABLE 3 Target Base sequence (corresponding Experimental sequence (corresponding siRNA region  nucleotides of SEQ ID NO: 1)  nucleotides of SEQ ID NO: 1) siHSP47- human/rat sense 5′ GGACAGGCCUCUACAACUAUU (SEQ ID 5′ GGACAGGCCUCUACAACUAdTdT (SEQ C hsp47 NO: 3) ID NO: 5) [945-963] [945-963] anti- 5′ UAGUUGUAGAGGCCUGUCCUU (SEQ ID 5′ UAGUUGUAGAGGCCUGUCCdTdT (SEQ sense NO: 4) ID NO: 6) [945-963] [945-963] siHSP47- human/rat sense 5′ GGACAGGCCUCUACAACUACUACGA 5′ GGACAGGCCUCUACAACUACUACdGdA Cd hsp47 (SEQ ID NO: 7) (SEQ ID NO: 9) [945-969] [945-969] anti- 5′UCGUAGUAGUUGUAGAGGCCUGUCCUU 5′UCGUAGUAGUUGUAGAGGCCUGUCCUU sense (SEQ ID NO: 8) (SEQ ID NO: 10) [945-969] [945-969] siHSP47- human/rat sense 5′ CAGGCCUCUACAACUACUAUU (SEQ ID 5′ CAGGCCUCUACAACUACUAdTdT (SEQ 1 hsp47 NO: 11) ID NO: 13) [948-966] [948-966] anti- 5′ UAGUAGUUGUAGAGGCCUGUU (SEQ ID 5′ UAGUAGUUGUAGAGGCCUGdTdT (SEQ sense NO: 12) ID NO: 14) [948-966] [948-966] siHSP47- human sense 5′ CAGGCCUCUACAACUACUACGACGA 5′ CAGGCCUCUACAACUACUACGACdGdA 1d hsp47 (SEQ ID NO: 15) (SEQ ID NO: 17) [948-972] [948-972] anti- 5′ CGUCGUAGUAGUUGUAGAGGCCUGUU 5′ CGUCGUAGUAGUUGUAGAGGCCUGUU sense (SEQ ID NO: 16) (SEQ ID NO: 18) [948-972] [948-972] siHsp47- human sense 5′ GAGCACUCCAAGAUCAACUUU (SEQ ID 5′ GAGCACUCCAAGAUCAACUdTdT (SEQ 2 hsp47 NO: 19) ID NO: 21) [698-717] [698-717] anti- 5′ AGUUGAUCUUGGAGUGCUCUU (SEQ ID 5′ AGUUGAUCUUGGAGUGCUCdTdT (SEQ sense NO: 20) ID NO: 22) [698-716] [698-716] siHsp47- human sense 5′ GAGCACUCCAAGAUCAACUUCCGCG 5′ GAGCACUCCAAGAUCAACUUCCGdCdG 2d hsp47 (SEQ ID NO: 23) (SEQ ID NO: 25) [698-722] [698-722] anti- 5′CGCGGAAGUUGAUCUUGGAGUGCUCUU 5′ GCGGAAGUUGAUCUUGGAGUGCUCUU sense (SEQ ID NO: 24) (SEQ ID NO: 26) [698-722] [698-722] siHsp47- rat Gp46 sense 5′ GAACACUCCAAGAUCAACUUCCGAG 5′ GAACACUCCAAGAUCAACUUCCGdAdG 2d rat (SEQ ID NO: 27) (SEQ ID NO: 29) [587-611] [587-611] anti- 5′CUCGGAAGUUGAUCUUGGAGUGUUCUU 5′ UCGGAAGUUGAUCUUGGAGUGUUCUU sense (SEQ ID NO: 28) (SEQ ID NO: 30) [587-611] [587-611] siHsp47- human sense 5′ CUGAGGCCAUUGACAAGAAUU (SEQ ID 5′ CUGAGGCCAUUGACAAGAAdTdT (SEQ 3 hsp47 NO: 31) ID NO: 33) [1209-1227] [1209-1227] anti- 5′ UUCUUGUCAAUGGCCUCAGUU (SEQ ID 5′ UUCUUGUCAAUGGCCUCAGdTdT (SEQ sense NO: 32) ID NO: 34) [1209-1227] [1209-1227] siHsp47- human sense 5′ CUGAGGCCAUUGACAAGAACAAGGC 5′ CUGAGGCCAUUGACAAGAACAAGdGdC 3d hsp47 (SEQ ID NO: 35) (SEQ ID NO: 37) [1209-1233] [1209-1233] anti- 5′ CCUUGUUCUUGUCAAUGGCCUCAGUU 5′GCCUUGUUCUUGUCAAUGGCCUCAGUU sense (SEQ ID NO: 36) (SEQ ID NO: 38) [1209-1233] [1209-1233] siHsp47- human sense 5′ CUACGACGACGAGAAGGAAUU (SEQ ID 5′ CUACGACGACGAGAAGGAAdTdT (SEQ 4 hsp47 NO: 39) ID NO: 41) [964-982] [964-982] anti- 5′ UUCCUUCUCGUCGUCGUAGUU (SEQ ID 5′ UUCCUUCUCGUCGUCGUAGdTdT (SEQ sense NO: 40) ID NO: 42) [964-982] [964-982] siHsp47- human sense 5′ CUACGACGACGAGAAGGAAAAGCUG 5′ CUACGACGACGAGAAGGAAAAGCdTdG 4d hsp47 (SEQ ID NO: 43) (SEQ ID NO: 45) [964-988] [964-988] anti- 5′ AGCUUUUCCUUCUCGUCGUCGUAGUU 5′ AGCUUUUCCUUCUCGUCGUCGUAGUU sense (SEQ ID NO: 44) (SEQ ID NO: 46) [964-988] [964-988] siHsp47- human sense 5′ GCCACACUGGGAUGAGAAAUU (SEQ ID 5′ GCCACACUGGGAUGAGAAAdTdT (SEQ 5 hsp47 NO: 47) ID NO: 49) [850-870] [850-870] anti- 5′ UUUCUCAUCCCAGUGUGGCUU (SEQ ID 5′ UUUCUCAUCCCAGUGUGGCdTdT (SEQ sense NO: 48) ID NO: 50) [850-868] [850-868] siHsp47- human sense 5′ GCAGCAAGCAGCACUACAAUU (SEQ ID 5′ GCAGCAAGCAGCACUACAAdTdT (SEQ 6 hsp47 NO: 51) ID NO: 53) [675-693] [675-693] anti- 5′ UUGUAGUGCUGCUUGCUGCUU (SEQ ID 5′ UUGUAGUGCUGCUUGCUGCdTdT (SEQ sense NO: 52) ID NO: 54) [675-693] [675-693] siHsp47- human sense 5′ CCGUGGGUGUCAUGAUGAUUU (SEQ ID 5′ CCGUGGGUGUCAUGAUGAUdTdT (SEQ 7 hsp47 NO: 55) ID NO: 57) [921-939] [921-939] anti- 5′ AUCAUCAUGACACCCACGGUU (SEQ ID 5′ AUCAUCAUGACACCCACGGdTdT (SEQ sense NO: 56) ID NO: 58) [921-939] [921-939]

Example 13

In order to screen for the potent of various siNA molecules against both the human and rat hsp47 genes, various reporter cell lines were established by lenti-viral induction of human HSP47 cDNA-green fluorescent protein (GFP) or rat GP46 cDNA-GFP construct into 293, HT1080, human HSC line hTERT, or NRK cell lines. These cell lines were further evaluated by siRNA against GFP. The remaining fluorescence signal was measured and normalized to scrambled siRNA (Ambion) and subsequently normalized to cell viability. The results showed that siRNA against GFP knocks down the fluorescence to different extent in different cell lines (FIG. 25). 293_HSP47-GFP and 293_GP46-GFP cell lines were selected for siHsp47 screening due to their ease of transfection and sensitivity to fluorescence knockdown.

Cells were transfected with 1.5 pmol per well of siNA against GFP in 96-well tissue culture plates using Lipofectamine RNAiMAX (Invitrogen) in a reverse transfection manner. Cells were seeded at 6,000 cells per well and mixed with the siNA complexes. Fluorescence readings were taken after 72 hours incubation on a Synergy 2 Multi-Mode Microplate Reader (BioTek).

Cells treated with or without siNA were measured for viability after 72 hours incubation using CellTiter-Glo Luminescent Cell Viability Assay Kit according to the manual (Promega). The readings were normalized to samples treated with scrambled siNA molecules.

Example 14 Evaluation of Inhibitory Efficiency of siHsp47 on Hsp47 Expression in Reporter Cell Lines

siNAs against hsp47 were evaluated for their inhibitory efficiency in 293_HSP47-GFP and 293_GP46-GFP cell lines by evaluating the change in fluorescent signal from the reporter GFP. The experiments were carried out as described in Example 2. The fluorescent signals were normalized to fluorescent signals from cells treated with scrambled siRNA (Ambion) which served as a control. The results indicate that the tested hsp47 siNA molecules were effective in inhibiting hsp47 mRNA in both cell lines. However, siNA against GP46 mRNA (as published in the 2008 Sato et al paper) was effective only in the 293_GP46-GFP cell line. The results are shown in FIGS. 26A and 26B.

The 293_HSP47-GFP and 293_GP46-GFP cell lines treated with siRNA against hsp47 and gp46 were evaluated for viability using the methods described in Example 2. The cell viability was normalized to cells treated with scrambled siRNA (Ambion). The results indicate that the cell viability was not affected significantly by the treatment with siNA molecules. However, the cell viability of 293_HSP47-GFP cell lines treated with different hsp47 siNA molecules varied depending on the siNA molecules used, while the viability of 293_GP46-GFP cell lines were similar. Viability for 293_HSP47-GFP cells was lower for siHsp47-6 and Hsp47-7 treated cells than the rest. The results are shown in FIGS. 26C and 26D.

Example 15 Evaluation of siHsp47 Inhibitory Effect on Hsp47 mRNA by TaqMan® qPCR

In Example 14, the knock down efficiency of siHsp47s in reporter cell lines was evaluated by change in fluorescent signal. To validate the results at the mRNA level, siRNAs targeting endogenous hsp47 were transfected into cells of the human HSC cell line hTERT using Lipofectamine RNAiMAX (Invitrogen) in a reverse transfection manner as described in Example 13

The hsp47 mRNA level was evaluated for knock down efficiency of the various tested siHsp47 siNA molecules. Briefly, mRNA were isolated from hTERT cells after 72 hours after transfection using an RNeasy mini kit (Qiagen). The level of hsp47 mRNA was determined by reverse transcription coupled with quantitative PCR using TaqMan® probes. Briefly, cDNA synthesis was carried out using High-Capacity cDNA Reverse Transcription Kit (ABI) according to the manufacturer's instruction, and subjected to TaqMan Gene Expression Assay (ABI, hsp47). The level of hsp47 mRNA was normalized to the level of GAPDH mRNA according to the manufacturer's instruction (ABI). The results indicate that siHsp47-C was the most effective among all the hsp47 siRNAs, siHsp47-2 and siHsp47-2d were the next most effective. The combinations of siHsp47-1 with siHsp47-2 or siHsp47-1 with siHsp47-2d were more effective than siHsp47-1 alone. The results are shown in FIG. 27.

Example 16 Validation of siHsp47 Knock Down Effect at the Protein Level

The inhibitory effect of different Hsp47 siNA molecules (siHsp47) on hsp47 mRNA expression were validated at the protein level by measuring the HSP47 in hTERT cells transfected with different siHsp47. Transfection of hTERT cells with different siHsp47 were performed as described in Example 13. Transfected hTERT cells were lysed and the cell lysate were clarified by centrifugation. Proteins in the clarified cell lysate were resolved by SDS polyacrylamide gel electrophoresis. The level of HSP47 protein in the cell lysate were determined using an anti-HSP47 antibody (Assay Designs) as the primary antibody, Goat anti-mouse IgG conjugated with HRP (Millipore) as the secondary antibody, and subsequently detected by Supersignal West Pico Chemiluminescence kit (Pierce). Anti-actin antibody (Abcam) was used as a protein loading control. The result showed significant decrease in the level of Hsp47 protein in cells treated with siHsp47-C, siHsp47-2d, alone or combination of siHsp47-1 with siHsp47-2d.

Example 17 Downregulation of Collagen I Expression by Hsp47 siRNA

To determine the effect of siHsp47 on collagen I expression level, collagen I mRNA level in hTERT cells treated with different siRNA against hsp47 was measured. Briefly, hTERT cells were transfected with different siHsp47 as described in Example 13. The cells were lysed after 72 hours and mRNA were isolated using RNeasy mini kit according to the manual (Qiagen). The level of collagen 1 mRNA was determined by reverse transcription coupled with quantitative PCR using TaqMan® probes. Briefly, cDNA synthesis was carried out using High-Capacity cDNA Reverse Transcription Kit (ABI) according to the manual, and subjected to TaqMan Gene Expression Assay (ABI, COL1A1 assay). The level of collagen I mRNA was normalized to the level of GAPDH mRNA according to the manufacturer's instruction (ABI). The signals were normalized to the signal obtained from cells transfected with scrambled siNA. The result indicated that collagen I mRNA level is significantly reduced in the cells treated with some of the candidates siHsp47-2, siHsp47-2d, and their combination with siHsp47-1 and shown in FIG. 28.

Example 18 Immunofluorescence Staining of Hsp47 siRNA Treated hTERT Cells

To visualize the expression of two fibrosis markers, collagen I and alpha-smooth muscle actin (SMA), in hTERT cells transfected with or without siHsp47, the cells were stained with rabbit anti-collagen I antibody (Abcam) and mouse anti-alpha-SMA antibody (Sigma). Alexa Fluor 594 goat anti-mouse IgG and Alexa Fluor 488 goat anti-rabbit IgG (Invitrogen (Molecular Probes)) were used as secondary antibodies to visualize collagen I (green) and alpha-SMA (red). Hoescht was used to visualize nuleus (blue). The results indicate correlation between siRNA knocking down of some of the target genes and collagen/SMA expression.

Example 19 In Vivo Testing of siHSP47 in Animal Models of Liver Fibrosis

The siRNA duplex sequence for HSP47 (siHSP47C) is as listed below.

(SEQ ID NO: 5) Sense (5′->3′) ggacaggccucuacaacuaTT (SEQ ID NO: 6) Antisense (5′->3′) uaguuguagaggccuguccTT

10 mg/ml siRNA stock solution was prepared by dissolving in nuclease free water (Ambion). For treatment of cirrhotic rats, siRNA was formulated with vitamin A-coupled liposome as described by Sato et al (Sato Y. et al. 2008 Nature Biotech. 26:431) in order to target activated HSC that produce collagen. The vitamin A (VA)-liposome-siRNA formulation consists of 0.33 μmol/m1 of VA, 0.33 μmol/m1 of liposome (Coatsome EL, NOF Corporation) and 0.5 μg/μl of siRNA in 5% glucose solution.

Four week-old male SD rats were induced with liver cirrhosis with 0.5% dimethylnitrosoamine (DMN) (Wako Chemicals, Japan) in phosphate-buffered saline (PBS). A dose of 2 ml/kg per body weight was administered intraperitoneally for 3 consecutive days per week on days 0, 2, 4, 7, 9, 11, 14, 16, 18, 21, 23, 25, 28, 30, 32, 34, 36, 38 and 40

siRNA treatment: siRNA treatment was carried out from day 32 and for 5 intravenous injections. In detail, rats were treated with siRNA on day-32, 34, 36, 38 and 40. Then rats were sacrificed on day-42 or 43. 3 different siRNA doses (1.5 mg siRNA per kg body weight, 2.25 mg siRNA per kg body weight, 3.0 mg siRNA per kg body weight) were tested. Detail of tested groups and number of animals in each group are as follows:

1) Cirrhosis was induced by DMN injection, then 5% glucose was injected instead of siRNA) (n=10)

2) VA-Lip-siHSP47C 1.5 mg/kg (n=10)

3) VA-Lip-siHSP47C 2.25 mg/kg (n=10)

4) VA-Lip-siHSP47C 3.0 mg/Kg (n=10)

5) Sham (PBS was injected instead of DMN; 5% Glucose was injected instead of siRNA) (n=6)

6) No treatment control (Intact) (n=6)

VA-Lip refers to vitamin A—liposome complex.

Evaluation of therapeutic efficacy: On day 43, 2 out of 10 animals in the “diseased rat” group and 1 out of 10 animals in “VA-Lip-siHSP47C siRNA 1.5 mg/kg” died due to development of liver cirrhosis. The remainder of the animals survived. After rats were sacrificed, liver tissues were fixed in 10% formalin. Then, the left lobule of each liver was embedded in paraffin for histology. Tissue slides were stained with Sirius red, and hematoxylin and eosin (HE). Sirius red staining was employed to visualize collagen-deposits and to determine the level of cirrhosis. HE staining was for nuclei and cytoplasm as counter-staining. Each slide was observed under microscope (BZ-8000, Keyence Corp. Japan) and percentage of Sirius red-stained area per slide was determined by image analysis software attached to the microscope. At least 4 slides per each liver were prepared for image analysis, and whole area of each slide (slice of liver) was captured by camera and analyzed. Statistical analysis was carried out by Student's t-test.

Results: FIG. 29 shows the fibrotic areas. The area of fibrosis in “diseased rats” was higher than in the “sham” or “no treatment control” groups. Therefore, DMN treatment induced collagen deposition in the liver, which was a typical observation of liver fibrosis. However, the area of fibrosis was significantly reduced by the treatment of siRNA targeting HSP47 gene, compared with “disease rat” group (FIG. 29). This result indicates that siRNA as disclosed herein has a therapeutic efficacy in actual disease.

Additional siRNA compounds are tested in the liver fibrosis animal model, and were shown to reduce the fibrotic area in the liver.

Example 20 Generation of Sequences for Active Double-Stranded RNA Compounds to HSP47/SERPINH1 and Production of the siRNAs Shown in Tables 4, 5, B, C, D and E

Duplexes are generated by annealing complementary single-stranded oligonucleotides. In a laminar flow hood, a 500 μM stock olution of single-stranded oligonucleotide is prepared by diluting in WFI (water for injection). Actual ssRNA concentrations are determined by diluting each 500 μM ssRNA 1:200 using WFI, and measuring the OD using Nano Drop. The procedure is repeated 3 times and the average concentration is calculated. The stock solution was then diluted to a final concentration of 250 μM. Complementary single-strands were annealed by heating to 85° C. and allowing to cool to room temperature over at least 45 minutes. Duplexes were tested for complete annealing by testing 5 μl on a 20% polyacrylamide gel and staining. Samples were stored at −80° C.

Tables 4, 5, B, C, D and E provide siRNAs for HSP47/SERPINH1. For each gene there is a separate list of 19-mer siRNA sequences, which are prioritized based on their score in the proprietary algorithm as the best sequences for targeting the human gene expression.

The following abbreviations are used in the Tables 4, 5, B, C, D and E herein: “other spec or Sp.” refers to cross species identity with other animals: D—dog, Rt—rat, Rb—Rabbit, Rh—rhesus monkey, P—Pig, M—Mouse; ORF: open reading frame. 19-mers (Tables 5, B, C), and 18+1—(Tables 4, D, E) mers refer to oligomers of 19 and 18+1 (U in position 1 of Antisense, A in position 19 of sense strand) ribonucleic acids in length, respectively.

siRNA oligonucleotides useful in generating double-stranded RNA molecules are disclosed in Tables 4, 5, B, C, D and E below.

TABLE 4 SEQ SEQ ID ID NO NO Cross Ident Human Name SEN Sense (5′ > 3′) AS Antisense (5′ > 3′) Species gi″32454740 SERPINH1_2 2734 GAGACACAUGGGUGCUAUA 2735 UAUAGCACCCAUGUGUCUC H, Rt, Rh, [1533-1551] M, D (18/19) SERPINH1_3 61 GGGAAGAUGCAGAAGAAGA 128 UCUUCUUCUGCAUCUUCCC H, Rt, Rh, [1112-1130] Rb (18/19) SERPINH1_5 62 GAAGAAGGCUGUUGCCAUA 129 UAUGGCAACAGCCUUCUUC H, Rt [1123-1141] (18/19) SERPINH1_6 2736 ACAAGAUGCGAGACGAGUA 2737 UACUCGUCUCGCAUCUUGU H, Rt, Rh, [1464-1482] (18/19) SERPINH1_7 64 GGACAACCGUGGCUUCAUA 131 UAUGAAGCCACGGUUGUCC H, Rh, M [886-904] (18/19) SERPINH1_8 65 UGCAGUCCAUCAACGAGUA 132 UACUCGUUGAUGGACUGCA H, Rt, Rh, [738-756] M (18/19) SERPINH1_9 66 GCCUCAUCAUCCUCAUGCA 133 UGCAUGAGGAUGAUGAGGC H, Rt, Rh, [1026-1044] M, D (18/19) SERPINH1_10 67 CGCGCUGCAGUCCAUCAAA 134 UUUGAUGGACUGCAGCGCG H, Rt, Rh [733-751] (18/19) SERPINH1_11 68 CGGACAGGCCUCUACAACA 135 UGUUGUAGAGGCCUGUCCG H, Rt, Rh, [944-962] P (18/19) SERPINH1_13 69 UGACAAGAUGCGAGACGAA 136 UUCGUCUCGCAUCUUGUCA H, Rh [1462-1480] (18/19) SERPINH1_14 70 CCAGCCUCAUCAUCCUCAA 137 UUGAGGAUGAUGAGGCUGG H, M, Rt, [1023-1041] Rh, D- (18/19) SERPINH1_15 71 GCUGCAGUCCAUCAACGAA 138 UUCGUUGAUGGACUGCAGC H, Rt, Rh [736-754] (18/19) SERPINH1_16 72 GCAGCGCGCUGCAGUCCAA 139 UUGGACUGCAGCGCGCUGC H, Rt, Rh [729-747] (18/19) SERPINH1_17 73 UGAGACACAUGGGUGCUAA 140 UUAGCACCCAUGUGUCUCA H, Rt, Rh [1532-1550] M, D (18/19) SERPINH1_19 74 GGUGGAGGUGACCCAUGAA 141 UUCAUGGGUCACCUCCACC H, Rt, Rh, [1159-1177] M (18/19) SERPINH1_20 75 CUUUGACCAGGACAUCUAA 142 UUAGAUGUCCUGGUCAAAG H, Rt, Rh [1324-1342] (18/19) SERPINH1_21 76 GGAGGUGACCCAUGACCUA 143 UAGGUCAUGGGUCACCUCC H, Rt, Rh, [1162-1180] M, D (18/19) SERPINH1_22 77 CUCCUGAGACACAUGGGUA 144 UACCCAUGUGUCUCAGGAG H, D [1528-1546] (18/19) SERPINH1_23 78 AGAAGAAGGCUGUUGCCAA 145 UUGGCAACAGCCUUCUUCU H, Rt [1122-1140] (18/19) SERPINH1_24 79 AGCUCUCCAGCCUCAUCAA 146 UUGAUGAGGCUGGAGAGCU H, Rt, D, [1017-1035] M, P, Rh (18/19) SERPINH1_25 80 CUGCAGUCCAUCAACGAGA 147 UCUCGUUGAUGGACUGCAG H, Rt, Rh [737-755] M (18/19) SERPINH1_26 81 CCGGACAGGCCUCUACAAA 148 UUUGUAGAGGCCUGUCCGG H, Rt, Rh, [943-961] Rb, P (18/19) SERPINH1_27 82 GCACCGGACAGGCCUCUAA 149 UUAGAGGCCUGUCCGGUGC H, Rt, Rh, [940-958] Rb, P (18/19) SERPINH1_28 83 GCAGAAGAAGGCUGUUGCA 150 UGCAACAGCCUUCUUCUGC H, Rt [1120-1138] (18/19) SERPINH1_31 84 AGAAGGCUGUUGCCAUCUA 151 UAGAUGGCAACAGCCUUCU H, Rt [1125-1143] (18/19) SERPINH1_32 85 AGCGCAGCGCGCUGCAGUA 152 UACUGCAGCGCGCUGCGCU H, Rt, Rh, [726-744] (18/19) SERPINH1_33 86 GACACAUGGGUGCUAUUGA 153 UCAAUAGCACCCAUGUGUC H, Rt, Rh, [1535-1553] M (18/19)| SERPINH1_34 87 GGGCCUGACUGAGGCCAUA 154 UAUGGCCUCAGUCAGGCCC H, Rt [1201-1219] (18/19) SERPINH1_35 88 AGACACAUGGGUGCUAUUA 155 UAAUAGCACCCAUGUGUCU H, Rt, Rh, [1534-1552] M (18/19) SERPINH1_36 89 CCAUGACCUGCAGAAACAA 156 UUGUUUCUGCAGGUCAUGG H, Rt, Rh, [1171-1189] M (18/19) SERPINH1_37 90 AGAUGCAGAAGAAGGCUGA 157 UCAGCCUUCUUCUGCAUCU H, Rt, Rh, [1116-1134] M (18/19) SERPINH1_38 91 CAAGCUCUCCAGCCUCAUA 158 UAUGAGGCUGGAGAGCUUG H, Rt, Rh, [1015-1033] M, P, D (18/19) SERPINH1_39 92 UGCAGAAGAAGGCUGUUGA 159 UCAACAGCCUUCUUCUGCA H, Rt [1119-1137] (18/19) SERPINH1_41 93 CAGCCUCAUCAUCCUCAUA 160 UAUGAGGAUGAUGAGGCUG H, Rt, Rh, [1024-1042] M, D (18/19) SERPINH1_42 94 GACAGGCCUCUACAACUAA 161 UUAGUUGUAGAGGCCUGUC H, Rt, Rh, [946-964] Rb, P (18/19) SERPINH1_43 95 GAUGCAGAAGAAGGCUGUA 162 UACAGCCUUCUUCUGCAUC H, Rt, Rh, [1117-1135] M (18/19) SERPINH1_44 96 ACCCAUGACCUGCAGAAAA 163 UUUUCUGCAGGUCAUGGGU H, Rt, Rh, [1169-1187] M (18/19) SERPINH1_45 97 ACUCCAAGAUCAACUUCCA 164 UGGAAGUUGAUCUUGGAGU H, Rt, Rh, [702-720] M, D (18/19) SERPINH1_45a 2738 ACUCCAAGAUCAACUUCCU 2739 AGGAAGUUGAUCUUGGAGU H, Rt, Rh, [702-720] M, D (18/19) SERPINH1_48 99 AGGCCUCUACAACUACUAA 166 UUAGUAGUUGUAGAGGCCU H, Rt, Rh, [949-967] Rb, P, D (18/19) SERPINH1_49 100 CACUCCAAGAUCAACUUCA 167 UGAAGUUGAUCUUGGAGUG H, Rt, Rh, [701-719] M, D (18/19) SERPINH1_51 2740 UCCUGAGACACAUGGGUGA 2741 UCACCCAUGUGUCUCAGGA H, Rt, D, [1529-1547] M (18/19) SERPINH1_52 102 GACAAGAUGCGAGACGAGA 169 UCUCGUCUCGCAUCUUGUC H, Rt, Rh, [1463-1481] (18/19) SERPINH1_53 103 GGUGACCCAUGACCUGCAA 170 UUGCAGGUCAUGGGUCACC H, Rt, Rh, [1165-1183] M (18/19) SERPINH1_59 104 CCGAGGUGAAGAAACCUGA 171 UCAGGUUUCUUCACCUCGG H, Rt, Rh, [285-303] (18/19) SERPINH1_51a 2742 UCCUGAGACACAUGGGUGU 2743 ACACCCAUGUGUCUCAGGA H, Rt, D, [1529-1547] M (18/19) SERPINH1_61 106 GCACUCCAAGAUCAACUUA 173 UAAGUUGAUCUUGGAGUGC H, Rh, D [700-718] (18/19) SERPINH1_62 107 GUGGUGGAGGUGACCCAUA 174 UAUGGGUCACCUCCACCAC H, Rt, Rh, [1157-1175] M, Rb (18/19) SERPINH1_64 108 GCCGAGGUGAAGAAACCUA 175 UAGGUUUCUUCACCUCGGC H, Rt, Rh, [284-302] (18/19) SERPINH1_65 109 GCUCUCCAGCCUCAUCAUA 176 UAUGAUGAGGCUGGAGAGC H, Rt, D, [1018-1036] M, P, Rh (18/19) SERPINH1_66 110 GAUGCACCGGACAGGCCUA 177 UAGGCCUGUCCGGUGCAUC H, Rt, Rh, [937-955] M, Rb, P (18/19) SERPINH1_68 111 CUCUCCAGCCUCAUCAUCA 178 UGAUGAUGAGGCUGGAGAG H, Rt, D, [1019-1037] M, P, Rh (18/19) SERPINH1_69 112 GCAGACCACCGACGGCAAA 179 UUUGCCGUCGGUGGUCUGC H, Rt, D [763-781] (18/19) SERPINH1_70 113 AGUCCAUCAACGAGUGGGA 180 UCCCACUCGUUGAUGGACU H, Rt, Rh, [741-759] M (18/19) SERPINH1_71 114 ACCGUGGCUUCAUGGUGAA 181 UUCACCAUGAAGCCACGGU H, Rt, Rh, [891-909] M (18/19) SERPINH1_74 115 GAAGGCUGUUGCCAUCUCA 182 UGAGAUGGCAACAGCCUUC H, Rt, [1126-1144] (18/19) SERPINH1_75 116 GAAGAUGCAGAAGAAGGCA 183 UGCCUUCUUCUGCAUCUUC H, Rt, Rh, [1114-1132] Rb (18/19) SERPINH1_77 117 UGAUGAUGCACCGGACAGA 184 UCUGUCCGGUGCAUCAUCA H, Rh, [933-951] (18/19) SERPINH1_78 118 CCCUUUGACCAGGACAUCA 185 UGAUGUCCUGGUCAAAGGG H, Rt, Rh, [1322-1340] (18/19) SERPINH1_80 119 CAGUCCAUCAACGAGUGGA 186 UCCACUCGUUGAUGGACUG H, Rt, Rh, [740-758] M (18/19) SERPINH1_82 120 CAACCGUGGCUUCAUGGUA 187 UACCAUGAAGCCACGGUUG H, Rt, Rh, [889-907] M (18/19) SERPINH1_83 121 CGACAAGCGCAGCGCGCUA 188 UAGCGCGCUGCGCUUGUCG H [721-739] (18/19) SERPINH1_84 122 GCAGUCCAUCAACGAGUGA 189 UCACUCGUUGAUGGACUGC H, Rt, Rh, [739-757] M (18/19) SERPINH1_86 123 ACAGGCCUCUACAACUACA 190 UGUAGUUGUAGAGGCCUGU H, Rt, Rh, [947-965] Rb, P, D (18/19) SERPINH1_87 124 AAGAUGCAGAAGAAGGCUA 191 UAGCCUUCUUCUGCAUCUU H, Rt, Rh, [1115-1133] M (18/19) SERPINH1_89 125 CAGCGCGCUGCAGUCCAUA 192 UAUGGACUGCAGCGCGCUG H, Rt, Rh, [730-748] (18/19) SERPINH1_90 126 GCGCAGCGCGCUGCAGUCA 193 UGACUGCAGCGCGCUGCGC H, Rt, Rh, [727-745] (18/19)

TABLE 5 SEQ  SEQ ID ID Ident NO NO Human gi Name SEN Sense (5′ > 3′) AS Antisense (5′ > 3′) Species Lg 32454740 SERPINH1_1 194 GGACAGGCCUCUACAACUA 219 UAGUUGUAGAGGCCUGUCC H, Rt, Rh, 19 [945-963] Rb, P (19/19) SERPINH1_4 2744 GAGACACAUGGGUGCUAUU 2745 AAUAGCACCCAUGUGUCUC H, Rt, Rh, 19 [1533-1551] m, D (19/19) SERPINH1_12 2746 ACAAGAUGCGAGACGAGUU 2747 AACUCGUCUCGCAUCUUGU H, Rt, Rh, 19 [1464-1482] (19/19) SERPINH1_18 197 CCUUUGACCAGGACAUCUA 222 UAGAUGUCCUGGUCAAAGG H, Rt, Rh, 19 [1323-1341] (19/19) SERPINH1_29 198 GACCCAUGACCUGCAGAAA 223 UUUCUGCAGGUCAUGGGUC H, Rt, Rh, 19 [1168-1186] M (19/19) SERPINH1_30 199 CGGACAGGCCUCUACAACU 224 AGUUGUAGAGGCCUGUCCG H, Rt, Rh, 19 [944-962] Rb, P (19/19) SERPINH1_40 200 ACCGGACAGGCCUCUACAA 225 UUGUAGAGGCCUGUCCGGU H, Rt, Rh, 19 [942-960] Rb, P,  (19/19) SERPINH1_46 201 GCAGCGCGCUGCAGUCCAU 226 AUGGACUGCAGCGCGCUGC H, Rt, Rh, 19 [729-747] (19/19) SERPINH1_47 202 GCGCGCUGCAGUCCAUCAA 227 UUGAUGGACUGCAGCGCGC H, Rt, Rh, 19 [732-750] (19/19) SERPINH1_50 203 CUGAGACACAUGGGUGCUA 228 UAGCACCCAUGUGUCUCAG H, Rt, Rh, 19 [1531-1549] M, D (19/19) SERPINH1_54 204 AGAAGAAGGCUGUUGCCAU 229 AUGGCAACAGCCUUCUUCU H, Rt 19 [1122-1140] (19/19) SERPINH1_55 205 AGCUCUCCAGCCUCAUCAU 230 AUGAUGAGGCUGGAGAGCU H, Rt, D, 19 [1017-1035] M, P, Rh (19/19) SERPINH1_56 206 CUGCAGUCCAUCAACGAGU 231 ACUCGUUGAUGGACUGCAG H, Rt, Rh, 19 [737-755] M (19/19) SERPINH1_57 207 CGCUGCAGUCCAUCAACGA 232 UCGUUGAUGGACUGCAGCG H, Rt, Rh, 19 [735-753] (19/19) SERPINH1_58 208 GACAAGAUGCGAGACGAGU 233 ACUCGUCUCGCAUCUUGUC H, Rt, Rh, 19 [1463-1481] (19/19) SERPINH1_63 209 GGGCCUGACUGAGGCCAUU 234 AAUGGCCUCAGUCAGGCCC H, Rt 19 [1201-1219] (19/19) SERPINH1_67 210 GAUGCAGAAGAAGGCUGUU 235 AACAGCCUUCUUCUGCAUC H, Rt, Rh, 19 [1117-1135] M (19/19) SERPINH1_72 211 CACCGGACAGGCCUCUACA 236 UGUAGAGGCCUGUCCGGUG H, Rt, Rh, 19 [941-959] Rb, P (19/19) SERPINH1_73 212 AGAUGCAGAAGAAGGCUGU 237 ACAGCCUUCUUCUGCAUCU H, Rt, Rh 19 [1116-1134] M (19/19) SERPINH1_76 213 AGCGCGCUGCAGUCCAUCA 238 UGAUGGACUGCAGCGCGCU H, Rt, Rh 19 [731-749] (19/19) SERPINH1_79 214 GGAAGAUGCAGAAGAAGGC 239 GCCUUCUUCUGCAUCUUCC H, Rt, Rh, 19 [1113-1131] Rb (19/19) SERPINH1_81 215 GAAGAAGGCUGUUGCCAUC 240 GAUGGCAACAGCCUUCUUC H, Rt 19 [1123-1141] (19/19) SERPINH1_85 216 UGCAGUCCAUCAACGAGUG 241 CACUCGUUGAUGGACUGCA H, Rt, Rh, 19 [738-756] M (19/19) SERPINH1_88 217 CCUGAGACACAUGGGUGCU 242 AGCACCCAUGUGUCUCAGG H, Rt, D, 19 [1530-1548] M (19/19) SERPINH1_91 218 CGCAGCGCGCUGCAGUCCA 243 UGGACUGCAGCGCGCUGCG H, Rt, Rh, 19 [728-746] (19/19)

TABLE B Additional Active 19-mer SERPINH1 siRNAs human- 32454740 Other ORF: 230- No SEQ ID SEN Sense siRNA SEQ ID AS AntiSense siRNA Species 1486 1 244 GGCAGACUCUGGUCAAGAA 460 UUCUUGACCAGAGUCUGCC Rh [2009-2027] 3′UTR 2 245 CAGUGAGGCGGAUUGAGAA 461 UUCUCAAUCCGCCUCACUG [1967-1985] 3′UTR 3 246 AGCCUUUGUUGCUAUCAAU 462 AUUGAUAGCAACAAAGGCU Rh [2117-2135] 3′UTR 4 247 CCAUGUUCUUCAAGCCACA 463 UGUGGCUUGAAGAACAUGG Rh, Rb, D [837-855] ORF 5 248 CCCUCUUCUGACACUAAAA 464 UUUUAGUGUCAGAAGAGGG [1850-1868] 3′UTR 6 249 CCUCAAUCAGUAUUCAUAU 465 AUAUGAAUACUGAUUGAGG [1774-1792] 3′UTR 7 250 GAGACACAUGGGUGCUAUU 466 AAUAGCACCCAUGUGUCUC Rh, D, Rt, M [1533-1551] 3′UTR 8 251 GUGACAAGAUGCGAGACGA 467 UCGUCUCGCAUCUUGUCAC Rh [1461-1479] ORF 9 252 GCCACACUGGGAUGAGAAA 468 UUUCUCAUCCCAGUGUGGC Rh, Rb, M [850-868] ORF 10 253 AGAUGCGAGACGAGUUAUA 469 UAUAACUCGUCUCGCAUCU Rh [1467-1485] ORF 11 254 ACGACGACGAGAAGGAAAA 470 UUUUCCUUCUCGUCGUCGU [966-984] ORF 12 255 GCCUCUACAACUACUACGA 471 UCGUAGUAGUUGUAGAGGC Rb, D [951-969] ORF 13 256 AGAUCAACUUCCGCGACAA 472 UUGUCGCGGAAGUUGAUCU D [708-726] ORF 14 257 ACUACUACGACGACGAGAA 473 UUCUCGUCGUCGUAGUAGU Rb [960-978] ORF 15 258 AGCCCUCUUCUGACACUAA 474 UUAGUGUCAGAAGAGGGCU [1848-1866] 3′UTR 16 259 ACAAGAUGCGAGACGAGUU 475 AACUCGUCUCGCAUCUUGU Rh, Rt [1464-1482] ORF 17 260 AGCCACACUGGGAUGAGAA 476 UUCUCAUCCCAGUGUGGCU Rh, Rb, M [849-867] ORF 18 261 AGGACCAGGCAGUGGAGAA 477 UUCUCCACUGCCUGGUCCU Rh [408-426] ORF 19 262 CAGGCAAGAAGGACCUGUA 478 UACAGGUCCUUCUUGCCUG Rh, D [1251-1269] ORF 20 263 ACCUGUGAGACCAAAUUGA 479 UCAAUUUGGUCUCACAGGU Rh [1813-1831] 3′UTR 21 264 CUUUGUUGCUAUCAAUCCA 480 UGGAUUGAUAGCAACAAAG Rh [2120-2138] 3′UTR 22 265 GUGAGACCAAAUUGAGCUA 481 UAGCUCAAUUUGGUCUCAC Rh [1817-1835] 3′UTR 23 266 CCCUGAAAGUCCCAGAUCA 482 UGAUCUGGGACUUUCAGGG [1749-1767] 3′UTR 24 267 CCUUUGACCAGGACAUCUA 483 UAGAUGUCCUGGUCAAAGG Rh, Rt [1323-1341] ORF 25 268 GACCAGGCAGUGGAGAACA 484 UGUUCUCCACUGCCUGGUC Rh [410-428] ORF 26 269 CGCGCAACGUGACCUGGAA 485 UUCCAGGUCACGUUGCGCG M [597-615] ORF 27 270 AUGAGAAAUUCCACCACAA 486 UUGUGGUGGAAUUUCUCAU Rh [861-879] ORF 28 271 GAAGAAACCUGCAGCCGCA 487 UGCGGCUGCAGGUUUCUUC [292-310] ORF 29 272 CUCUCGAGCGCCUUGAAAA 488 UUUUCAAGGCGCUCGAGAG [1059-1077] ORF 30 273 GGAACAUGAGCCUUUGUUG 489 CAACAAAGGCUCAUGUUCC Rh [2109-2127] 3′UTR 31 274 CUCACCUGUGAGACCAAAU 490 AUUUGGUCUCACAGGUGAG Rh [1810-1828] 3′UTR 32 275 CUACGACGACGAGAAGGAA 491 UUCCUUCUCGUCGUCGUAG Rb [964-982] ORF 33 276 ACCACAAGAUGGUGGACAA 492 UUGUCCACCAUCUUGUGGU Rh, Rb, M, P [873-891] ORF 34 277 CUGGCACUGCGGAGAAGUU 493 AACUUCUCCGCAGUGCCAG [318-336] ORF 35 278 GGUCCUAUACCGUGGGUGU 494 ACACCCACGGUAUAGGACC Rh [912-930] ORF 36 279 CCAACCUCUCCCAACUAUA 495 UAUAGUUGGGAGAGGUUGG Rh [1896-1914] 3′UTR 37 280 GAGAAGGAAAAGCUGCAAA 496 UUUGCAGCUUUUCCUUCUC Rh [974-992] ORF 38 281 GCCUCUCGAGCGCCUUGAA 497 UUCAAGGCGCUCGAGAGGC [1057-1075] ORF 39 282 AGGCCAUUGACAAGAACAA 498 UUGUUCUUGUCAAUGGCCU Rh, D [1212-1230] ORF 40 283 GACCCAUGACCUGCAGAAA 499 UUUCUGCAGGUCAUGGGUC Rh, Rt, M [1168-1186] ORF 41 284 CUCCUGGCACUGCGGAGAA 500 UUCUCCGCAGUGCCAGGAG [315-333] ORF 42 285 CGGACAGGCCUCUACAACU 501 AGUUGUAGAGGCCUGUCCG Rh, Rb, Rt, P [944-962] ORF 43 286 GAUGAGAAAUUCCACCACA 502 UGUGGUGGAAUUUCUCAUC Rh [860-878] ORF 44 287 CACGCAUGUCAGGCAAGAA 503 UUCUUGCCUGACAUGCGUG Rh, D [1242-1260] ORF 45 288 ACCUCUCCCAACUAUAAAA 504 UUUUAUAGUUGGGAGAGGU Rh [1899-1917] 3′UTR 46 289 ACCAGGCAGUGGAGAACAU 505 AUGUUCUCCACUGCCUGGU Rh [411-429] ORF 47 290 GGGAACAUGAGCCUUUGUU 506 AACAAAGGCUCAUGUUCCC Rh [2108-2126] 3′UTR 48 291 AGAAUUCACUCCACUUGGA 507 UCCAAGUGGAGUGAAUUCU Rh [1653-1671] 3′UTR 49 292 GGGCAGACUCUGGUCAAGA 508 UCUUGACCAGAGUCUGCCC Rh [2008-2026] 3′UTR 50 293 AGAAGGAAAAGCUGCAAAU 509 AUUUGCAGCUUUUCCUUCU Rh [975-993] ORF 51 294 GGCAGUGGAGAACAUCCUG 510 CAGGAUGUUCUCCACUGCC Rh [415-433] ORF 52 295 GGGAUGAGAAAUUCCACCA 511 UGGUGGAAUUUCUCAUCCC Rh [858-876] ORF 53 296 CCAAGCUGUUCUACGCCGA 512 UCGGCGUAGAACAGCUUGG Rh [1365-1383] ORF 54 297 ACCGGACAGGCCUCUACAA 513 UUGUAGAGGCCUGUCCGGU Rh, Rb, Rt, P [942-960] ORF 55 298 CUGCCUCAAUCAGUAUUCA 514 UGAAUACUGAUUGAGGCAG [1771-1789] 3′UTR 56 299 CAGCCCUCUUCUGACACUA 515 UAGUGUCAGAAGAGGGCUG [1847-1865] 3′UTR 57 300 CCAGCCUCAUCAUCCUCAU 516 AUGAGGAUGAUGAGGCUGG Rh, D, Rt, M [1023-1041] ORF 58 301 AGGGUGACAAGAUGCGAGA 517 UCUCGCAUCUUGUCACCCU Rh, D [1458-1476] ORF 59 302 GGACCAGGCAGUGGAGAAC 518 GUUCUCCACUGCCUGGUCC Rh [409-427] ORF 60 303 GCAGCGCGCUGCAGUCCAU 519 AUGGACUGCAGCGCGCUGC Rh, Rt [729-747] ORF 61 304 GCGCGCUGCAGUCCAUCAA 520 UUGAUGGACUGCAGCGCGC Rh, Rt [732-750] ORF 62 305 CCAGAUACCAUGAUGCUGA 521 UCAGCAUCAUGGUAUCUGG Rh [1680-1698] 3′UTR 63 306 CUAGUGCGGGACACCCAAA 522 UUUGGGUGUCCCGCACUAG [1400-1418] ORF 64 307 AGGCAGUGGAGAACAUCCU 523 AGGAUGUUCUCCACUGCCU Rh [414-432] ORF 65 308 CUGAGACACAUGGGUGCUA 524 UAGCACCCAUGUGUCUCAG Rh, D, Rt, M [1531-1549] 3′UTR 66 309 GAUUGAGAAGGAGCUCCCA 525 UGGGAGCUCCUUCUCAAUC [1977-1995] 3′UTR 67 310 CGCAGACCACCGACGGCAA 526 UUGCCGUCGGUGGUCUGCG D, Rt [762-780] ORF 68 311 CCACACUGGGAUGAGAAAU 527 AUUUCUCAUCCCAGUGUGG Rh [851-869] ORF 69 312 GCUCAGUGAGCUUCGCUGA 528 UCAGCGAAGCUCACUGAGC [642-660] ORF 70 313 CGCCUUUGAGUUGGACACA 529 UGUGUCCAACUCAAAGGCG Rh [1294-1312] ORF 71 314 GGGUCAGCCAGCCCUCUUC 530 GAAGAGGGCUGGCUGACCC Rh [1839-1857] 3′UTR 72 315 GGGCUUCUGGGCAGACUCU 531 AGAGUCUGCCCAGAAGCCC Rh [2000-2018] 3′UTR 73 316 GGUACCUUCUCACCUGUGA 532 UCACAGGUGAGAAGGUACC Rh [1802-1820] 3′UTR 74 317 GCCUGCCUCAAUCAGUAUU 533 AAUACUGAUUGAGGCAGGC [1769-1787] 3′UTR 75 318 UCUACAACUACUACGACGA 534 UCGUCGUAGUAGUUGUAGA Rb [954-972] ORF 76 319 GGGAAGAUGCAGAAGAAGG 535 CCUUCUUCUGCAUCUUCCC Rh, Rb, Rt [1112-1130] ORF 77 320 CGAGAAGGAAAAGCUGCAA 536 UUGCAGCUUUUCCUUCUCG Rh [973-991] ORF 78 321 AGAAGAAGGCUGUUGCCAU 537 AUGGCAACAGCCUUCUUCU Rt [1122-1140] ORF 79 322 CACAAGCUCUCCAGCCUCA 538 UGAGGCUGGAGAGCUUGUG Rh, D, M, P [1013-1031] ORF 80 323 GGGUGACAAGAUGCGAGAC 539 GUCUCGCAUCUUGUCACCC Rh, D [1459-1477] ORF 81 324 UGUUGGAGCGUGGAAAAAA 540 UUUUUUCCACGCUCCAACA [2190-2208] 3′UTR 82 325 CUUUGAGUUGGACACAGAU 541 AUCUGUGUCCAACUCAAAG Rh [1297-1315] ORF 83 326 AGCUCUCCAGCCUCAUCAU 542 AUGAUGAGGCUGGAGAGCU Rh, D, Rt, M, [1017-1035] P ORF 84 327 AGCUGUUCUACGCCGACCA 543 UGGUCGGCGUAGAACAGCU Rh [1368-1386] ORF 85 328 CUGCAGUCCAUCAACGAGU 544 ACUCGUUGAUGGACUGCAG Rh, Rt, M [737-755] ORF 86 329 UACGACGACGAGAAGGAAA 545 UUUCCUUCUCGUCGUCGUA [965-983] ORF 87 330 CCUAGUGCGGGACACCCAA 546 UUGGGUGUCCCGCACUAGG [1399-1417] ORF 88 331 CUUCUCACCUGUGAGACCA 547 UGGUCUCACAGGUGAGAAG Rh [1807-1825] 3′UTR 89 332 AGUUGGACACAGAUGGCAA 548 UUGCCAUCUGUGUCCAACU [1302-1320] ORF 90 333 CAGUGGAGAACAUCCUGGU 549 ACCAGGAUGUUCUCCACUG Rh [417-435] ORF 91 334 CCAGCUAGAAUUCACUCCA 550 UGGAGUGAAUUCUAGCUGG Rh [1647-1665] 3′UTR 92 335 CGCUGCAGUCCAUCAACGA 551 UCGUUGAUGGACUGCAGCG Rh, Rt [735-753] ORF 93 336 CCAAGGACCAGGCAGUGGA 552 UCCACUGCCUGGUCCUUGG Rh [405-423] ORF 94 337 AGUUCUUCAAAGAUAGGGA 553 UCCCUAUCUUUGAAGAACU [2082-2100] 3′UTR 95 338 CGGACCUUCCCAGCUAGAA 554 UUCUAGCUGGGAAGGUCCG Rh [1638-1656] 3′UTR 96 339 GACAAGAUGCGAGACGAGU 555 ACUCGUCUCGCAUCUUGUC Rh, Rt [1463-1481] ORF 97 340 CCAAGAUCAACUUCCGCGA 556 UCGCGGAAGUUGAUCUUGG D [705-723] ORF 98 341 CCCAUCACGUGGAGCCUCU 557 AGAGGCUCCACGUGAUGGG Rh [1044-1062] ORF 99 342 CCAUGAUGCUGAGCCCGGA 558 UCCGGGCUCAGCAUCAUGG [1687-1705] 3′UTR 100 343 AGCCUGCCUCAAUCAGUAU 559 AUACUGAUUGAGGCAGGCU [1768-1786] 3′UTR 101 344 CGGCCUAAGGGUGACAAGA 560 UCUUGUCACCCUUAGGCCG Rh [1451-1469] ORF 102 345 GGGCCUGACUGAGGCCAUU 561 AAUGGCCUCAGUCAGGCCC Rt [1201-1219] ORF 103 346 UCACCUGUGAGACCAAAUU 562 AAUUUGGUCUCACAGGUGA Rh [1811-1829] 3′UTR 104 347 GAGGCCAUUGACAAGAACA 563 UGUUCUUGUCAAUGGCCUC Rh, D [1211-1229] ORF 105 348 GCUCCUGGCACUGCGGAGA 564 UCUCCGCAGUGCCAGGAGC [314-332] ORF 106 349 GGCGCCUGGUCCGGCCUAA 565 UUAGGCCGGACCAGGCGCC Rh [1440-1458] ORF 107 350 CCAGCCCUCUUCUGACACU 566 AGUGUCAGAAGAGGGCUGG [1846-1864] 3′UTR 108 351 ACUACGACGACGAGAAGGA 567 UCCUUCUCGUCGUCGUAGU Rb [963-981] ORF 109 352 CCUAUACCGUGGGUGUCAU 568 AUGACACCCACGGUAUAGG Rh, D, P [915-933] ORF 110 353 GACCCAGCUCAGUGAGCUU 569 AAGCUCACUGAGCUGGGUC [636-654] ORF 111 354 UGGGUGUCAUGAUGAUGCA 570 UGCAUCAUCAUGACACCCA Rh [924-942] ORF 112 355 CCAAGGGUGUGGUGGAGGU 571 ACCUCCACCACACCCUUGG Rh, D [1149-1167] ORF 113 356 AGGUCACCAAGGACGUGGA 572 UCCACGUCCUUGGUGACCU Rh, D [789-807] ORF 114 357 CCCUGGCCGCCGAGGUGAA 573 UUCACCUCGGCGGCCAGGG [276-294] ORF 115 358 AGCACUCCAAGAUCAACUU 574 AAGUUGAUCUUGGAGUGCU Rh, D [699-717] ORF 116 359 CCUGGCACUGCGGAGAAGU 575 ACUUCUCCGCAGUGCCAGG [317-335] ORF 117 360 GAUGCAGAAGAAGGCUGUU 576 AACAGCCUUCUUCUGCAUC Rh, Rt, M [1117-1135] ORF 118 361 CCCACAAGCUCUCCAGCCU 577 AGGCUGGAGAGCUUGUGGG Rh, D, P [1011-1029] ORF 119 362 CUCUUCUGACACUAAAACA 578 UGUUUUAGUGUCAGAAGAG [1852-1870] 3′UTR 120 363 ACGAGAAGGAAAAGCUGCA 579 UGCAGCUUUUCCUUCUCGU Rh [972-990] ORF 121 364 UGAAAAGCUGCUAACCAAA 580 UUUGGUUAGCAGCUUUUCA [1072-1090] ORF 122 365 UCUCACCUGUGAGACCAAA 581 UUUGGUCUCACAGGUGAGA Rh [1809-1827] 3′UTR 123 366 CAUGAUGAUGCACCGGACA 582 UGUCCGGUGCAUCAUCAUG Rh [931-949] ORF 124 367 GGAUUGAGAAGGAGCUCCC 583 GGGAGCUCCUUCUCAAUCC [1976-1994] 3′UTR 125 368 CCUUCAUCUUCCUAGUGCG 584 CGCACUAGGAAGAUGAAGG [1389-1407] ORF 126 369 GGCCUGGCCUUCAGCUUGU 585 ACAAGCUGAAGGCCAGGCC [374-392] ORF 127 370 GGUCAGCCAGCCCUCUUCU 586 AGAAGAGGGCUGGCUGACC Rh [1840-1858] 3′UTR 128 371 UUCUCACCUGUGAGACCAA 587 UUGGUCUCACAGGUGAGAA Rh [1808-1826] 3′UTR 129 372 CGCAGCAGCUCCUGGCACU 588 AGUGCCAGGAGCUGCUGCG [307-325] ORF 130 373 GCCAUGUUCUUCAAGCCAC 589 GUGGCUUGAAGAACAUGGC Rh, Rb, D [836-854] ORF 131 374 AGGCAGUGCUGAGCGCCGA 590 UCGGCGCUCAGCACUGCCU [510-528] ORF 132 375 CACCUGUGAGACCAAAUUG 591 CAAUUUGGUCUCACAGGUG Rh [1812-1830] 3′UTR 133 376 CACCGGACAGGCCUCUACA 592 UGUAGAGGCCUGUCCGGUG Rh, Rb, Rt, P [941-959] ORF 134 377 AGCUAGAAUUCACUCCACU 593 AGUGGAGUGAAUUCUAGCU Rh [1649-1667] 3′UTR 135 378 AGAUGCAGAAGAAGGCUGU 594 ACAGCCUUCUUCUGCAUCU Rh, Rt, M [1116-1134] ORF 136 379 CCCUGCUAGUCAACGCCAU 595 AUGGCGUUGACUAGCAGGG Rh [822-840] ORF 137 380 ACAACUACUACGACGACGA 596 UCGUCGUCGUAGUAGUUGU Rb [957-975] ORF 138 381 GCUCCUGAGACACAUGGGU 597 ACCCAUGUGUCUCAGGAGC D [1527-1545] 3′UTR 139 382 UGGAGAACAUCCUGGUGUC 598 GACACCAGGAUGUUCUCCA Rh [420-438] ORF 140 383 AGCGCGCUGCAGUCCAUCA 599 UGAUGGACUGCAGCGCGCU Rh, Rt [731-749] ORF 141 384 CGCCUUGAAAAGCUGCUAA 600 UUAGCAGCUUUUCAAGGCG [1067-1085] ORF 142 385 GCCUUUGUUGCUAUCAAUC 601 GAUUGAUAGCAACAAAGGC Rh [2118-2136] 3′UTR 143 386 CUCUACAACUACUACGACG 602 CGUCGUAGUAGUUGUAGAG Rb [953-971] ORF 144 387 CGCUCACUCAGCAACUCCA 603 UGGAGUUGCUGAGUGAGCG Rh [575-593] ORF 145 388 GGUACCAGCCUUGGAUACU 604 AGUAUCCAAGGCUGGUACC Rh [1571-1589] 3′UTR 146 389 GCCUGACUGAGGCCAUUGA 605 UCAAUGGCCUCAGUCAGGC Rh [1203-1221] ORF 147 390 UGAGCUUCGCUGAUGACUU 606 AAGUCAUCAGCGAAGCUCA Rh [648-666] ORF 148 391 CCAGCCUUGGAUACUCCAU 607 AUGGAGUAUCCAAGGCUGG Rh [1575-1593] 3′UTR 149 392 AAAGGCUCCUGAGACACAU 608 AUGUGUCUCAGGAGCCUUU [1523-1541] 3′UTR 150 393 UGACCCAUGACCUGCAGAA 609 UUCUGCAGGUCAUGGGUCA Rh, Rt, M [1167-1185] ORF 151 394 CCUGUGAGACCAAAUUGAG 610 CUCAAUUUGGUCUCACAGG Rh [1814-1832] 3′UTR 152 395 GCGGACCUUCCCAGCUAGA 611 UCUAGCUGGGAAGGUCCGC Rh [1637-1655] 3′UTR 153 396 GGAAGAUGCAGAAGAAGGC 612 GCCUUCUUCUGCAUCUUCC Rh, Rb, Rt [1113-1131] ORF 154 397 UGCCCAAGGGUGUGGUGGA 613 UCCACCACACCCUUGGGCA Rh, D [1146-1164] ORF 155 398 GGAGCCUCUCGAGCGCCUU 614 AAGGCGCUCGAGAGGCUCC [1054-1072] ORF 156 399 GACUCUGGUCAAGAAGCAU 615 AUGCUUCUUGACCAGAGUC Rh [2013-2031] 3′UTR 157 400 CAGGCAGUGGAGAACAUCC 616 GGAUGUUCUCCACUGCCUG Rh [413-431] ORF 158 401 CAAGCCUGCCUCAAUCAGU 617 ACUGAUUGAGGCAGGCUUG Rh [1766-1784] 3′UTR 159 402 CUGGAAGCUGGGCAGCCGA 618 UCGGCUGCCCAGCUUCCAG [610-628] ORF 160 403 GAAGAAGGCUGUUGCCAUC 619 GAUGGCAACAGCCUUCUUC Rt [1123-1141] ORF 161 404 GGGCGAGCUGCUGCGCUCA 620 UGAGCGCAGCAGCUCGCCC Rh [562-580] ORF 162 405 AAGCCACACUGGGAUGAGA 621 UCUCAUCCCAGUGUGGCUU Rh, Rb, M [848-866] ORF 163 406 GUGUGGUGGAGGUGACCCA 622 UGGGUCACCUCCACCACAC Rh, D [1155-1173] ORF 164 407 CCGCCUUUGAGUUGGACAC 623 GUGUCCAACUCAAAGGCGG Rh [1293-1311] ORF 165 408 GGCCAUUGACAAGAACAAG 624 CUUGUUCUUGUCAAUGGCC Rh, D [1213-1231] ORF 166 409 UGCCUCAAUCAGUAUUCAU 625 AUGAAUACUGAUUGAGGCA [1772-1790] 3′UTR 167 410 CCUUCCCAGCUAGAAUUCA 626 UGAAUUCUAGCUGGGAAGG Rh [1642-1660] 3′UTR 168 411 GGGACCUGGGCCAUAGUCA 627 UGACUAUGGCCCAGGUCCC [1721-1739] 3′UTR 169 412 CGAGGUGAAGAAACCUGCA 628 UGCAGGUUUCUUCACCUCG Rh [286-304] ORF 170 413 GCCUUUGAGUUGGACACAG 629 CUGUGUCCAACUCAAAGGC Rh [1295-1313] ORF 171 414 AGCGGACCUUCCCAGCUAG 630 CUAGCUGGGAAGGUCCGCU Rh [1636-1654] 3′UTR 172 415 CGCAUGUCAGGCAAGAAGG 631 CCUUCUUGCCUGACAUGCG Rh, D [1244-1262] ORF 173 416 ACAACUGCGAGCACUCCAA 632 UUGGAGUGCUCGCAGUUGU Rh, D [690-708] ORF 174 417 GAGGCGGAUUGAGAAGGAG 633 CUCCUUCUCAAUCCGCCUC [1971-1989] 3′UTR 175 418 GGCCGCCGAGGUGAAGAAA 634 UUUCUUCACCUCGGCGGCC [280-298] ORF 176 419 CAGCUCUAUCCCAACCUCU 635 AGAGGUUGGGAUAGAGCUG [1886-1904] 3′UTR 177 420 AGCUGGGCAGCCGACUGUA 636 UACAGUCGGCUGCCCAGCU [615-633] ORF 178 421 GCCAUUGACAAGAACAAGG 637 CCUUGUUCUUGUCAAUGGC Rh, D [1214-1232] ORF 179 422 CGCCAUGUUCUUCAAGCCA 638 UGGCUUGAAGAACAUGGCG Rh, Rb, P [835-853] ORF 180 423 CCGAGGUCACCAAGGACGU 639 ACGUCCUUGGUGACCUCGG Rh, D [786-804] ORF 181 424 GGACCCAGCUCAGUGAGCU 640 AGCUCACUGAGCUGGGUCC [635-653] ORF 182 425 CCAAUGACAUUUUGUUGGA 641 UCCAACAAAAUGUCAUUGG [2178-2196] 3′UTR 183 426 AGUGAGGCGGAUUGAGAAG 642 CUUCUCAAUCCGCCUCACU [1968-1986] 3′UTR 184 427 UGCAGUCCAUCAACGAGUG 643 CACUCGUUGAUGGACUGCA Rh, Rt, M [738-756] ORF 185 428 UGUCACGCAUGUCAGGCAA 644 UUGCCUGACAUGCGUGACA Rh, D [1239-1257] ORF 186 429 CGACGACGAGAAGGAAAAG 645 CUUUUCCUUCUCGUCGUCG [967-985] ORF 187 430 ACAAGAACAAGGCCGACUU 646 AAGUCGGCCUUGUUCUUGU Rh [1221-1239] ORF 188 431 CUUCAAGCCACACUGGGAU 647 AUCCCAGUGUGGCUUGAAG Rh, Rb, D [844-862] ORF 189 432 CCUGGGCCAUAGUCAUUCU 648 AGAAUGACUAUGGCCCAGG [1725-1743] 3′UTR 190 433 UUUGUUGGAGCGUGGAAAA 649 UUUUCCACGCUCCAACAAA [2188-2206] 3′UTR 191 434 AGAACAUCCUGGUGUCACC 650 GGUGACACCAGGAUGUUCU [423-441] ORF 192 435 ACGCCACCGCCUUUGAGUU 651 AACUCAAAGGCGGUGGCGU Rh [1287-1305] ORF 193 436 GUGAGGUACCAGCCUUGGA 652 UCCAAGGCUGGUACCUCAC Rh [1567-1585] 3′UTR 194 437 GCGCCUUCUGCCUCCUGGA 653 UCCAGGAGGCAGAAGGCGC [252-270] ORF 195 438 GCCUGGCCUUCAGCUUGUA 654 UACAAGCUGAAGGCCAGGC [375-393] ORF 196 439 CCCGGAAACUCCACAUCCU 655 AGGAUGUGGAGUUUCCGGG [1700-1718] 3′UTR 197 440 UCUUCAAGCCACACUGGGA 656 UCCCAGUGUGGCUUGAAGA Rh, Rb, D [843-861] ORF 198 441 UGUUGCUAUCAAUCCAAGA 657 UCUUGGAUUGAUAGCAACA Rh [2123-2141] 3′UTR 199 442 GAGUGGGCCGCGCAGACCA 658 UGGUCUGCGCGGCCCACUC [752-770] ORF 200 443 CCUGAGACACAUGGGUGCU 659 AGCACCCAUGUGUCUCAGG D, Rt, M [1530-1548] 3′UTR 201 444 AGCCGACUGUACGGACCCA 660 UGGGUCCGUACAGUCGGCU [623-641] ORF 202 445 GGGCCUCAGGGUGCACACA 661 UGUGUGCACCCUGAGGCCC [1486-1504] 3′UTR 203 446 ACUGGGAUGAGAAAUUCCA 662 UGGAAUUUCUCAUCCCAGU Rh [855-873] ORF 204 447 AGAAUGACCUGGCCGCAGU 663 ACUGCGGCCAGGUCAUUCU [1952-1970] 3′UTR 205 448 CAUAUUUAUAGCCAGGUAC 664 GUACCUGGCUAUAAAUAUG Rh [1788-1806] 3′UTR 206 449 AGGUGACCCAUGACCUGCA 665 UGCAGGUCAUGGGUCACCU Rh, Rt, M [1164-1182] ORF 207 450 GCGCUGCAGUCCAUCAACG 666 CGUUGAUGGACUGCAGCGC Rh, Rt [734-752] ORF 208 451 GGUGACAAGAUGCGAGACG 667 CGUCUCGCAUCUUGUCACC Rh [1460-1478] ORF 209 452 CUUCAAAGAUAGGGAGGGA 668 UCCCUCCCUAUCUUUGAAG [2086-2104] 3′UTR 210 453 AGCUGCAAAUCGUGGAGAU  669 AUCUCCACGAUUUGCAGCU Rh [984-1002] ORF 211 454 GUGGAGAACAUCCUGGUGU 670 ACACCAGGAUGUUCUCCAC Rh [419-437] ORF 212 455 GAACAAGGCCGACUUGUCA 671 UGACAAGUCGGCCUUGUUC Rh [1225-1243] ORF 213 456 CAUGAUGCUGAGCCCGGAA 672 UUCCGGGCUCAGCAUCAUG [1688-1706] 3′UTR 214 457 GCGCCUUGAAAAGCUGCUA 673 UAGCAGCUUUUCAAGGCGC Rh [1066-1084] ORF 215 458 GCAGACUCUGGUCAAGAAG 674 CUUCUUGACCAGAGUCUGC Rh [2010-2028] 3′UTR 216 459 CCAGGCAGUGGAGAACAUC 675 GAUGUUCUCCACUGCCUGG Rh [412-430] ORF

TABLE C Cross-Species 19-mer SERPINH1 siRNAs human- 32454740 ORF: 230- No. SEQ ID SEN Sense siRNA SEQ ID AS AntiSense siRNA Other Species 1486 1 676 CACUACAACUGCGAGCACU 973 AGUGCUCGCAGUUGUAGUG Rh, D [686-704] ORF 2 677 AACCGUGGCUUCAUGGUGA 974 UCACCAUGAAGCCACGGUU Rh, Rt, M [890-908] ORF 3 678 GGCAAGAAGGACCUGUACC 975 GGUACAGGUCCUUCUUGCC Rh, D, M [1253- 1271] ORF 4 679 GGUGGACAACCGUGGCUUC 976 GAAGCCACGGUUGUCCACC Rh, M [883-901] ORF 5 680 AGGCCAUGGCCAAGGACCA 977 UGGUCCUUGGCCAUGGCCU Rh, D [396-414] ORF 6 681 CGCAGCGCGCUGCAGUCCA 978 UGGACUGCAGCGCGCUGCG Rh, Rt [728-746] ORF 7 682 AGCAGCAAGCAGCACUACA 979 UGUAGUGCUGCUUGCUGCU Rh, D [674-692] ORF 8 683 GGCCUCUACAACUACUACG 980 CGUAGUAGUUGUAGAGGCC Rb, D [950-968] ORF 9 684 GAAGAUGCAGAAGAAGGCU 981 AGCCUUCUUCUGCAUCUUC Rh, Rb, Rt [1114- 1132] ORF 10 685 GGCUCCUGAGACACAUGGG 982 CCCAUGUGUCUCAGGAGCC D [1526- 1544] 3′UTR 11 686 AGCAAGCAGCACUACAACU 983 AGUUGUAGUGCUGCUUGCU Rh, D [677-695] ORF 12 687 GGAGGUGACCCAUGACCUG 984 CAGGUCAUGGGUCACCUCC Rh, Rt, M [1162- 1180] ORF 13 688 CCCUUUGACCAGGACAUCU 985 AGAUGUCCUGGUCAAAGGG Rh, Rt [1322- 1340] ORF 14 689 CUCCUGAGACACAUGGGUG 986 CACCCAUGUGUCUCAGGAG D [1528- 1546] 3′UTR 15 690 AAGGCUCCUGAGACACAUG 987 CAUGUGUCUCAGGAGCCUU D [1524- 1542] 3′UTR 16 691 CGCGCUGCAGUCCAUCAAC 988 GUUGAUGGACUGCAGCGCG Rh, Rt [733-751] ORF 17 692 AGGGUGUGGUGGAGGUGAC 989 GUCACCUCCACCACACCCU Rh, D [1152- 1170] ORF 18 693 AGCACUACAACUGCGAGCA 990 UGCUCGCAGUUGUAGUGCU Rh, D [684-702] ORF 19 694 GGCUCCCUGCUAUUCAUUG 991 CAAUGAAUAGCAGGGAGCC D [1421- 1439] ORF 20 695 GCGCGCAACGUGACCUGGA 992 UCCAGGUCACGUUGCGCGC M [596-614] ORF 21 696 GCUGCAGUCCAUCAACGAG 993 CUCGUUGAUGGACUGCAGC Rh, Rt [736-754] ORF 22 697 ACCAAAGAGCAGCUGAAGA 994 UCUUCAGCUGCUCUUUGGU Rh, Rb, P [1085- 1103] ORF 23 698 CCAAGGACGUGGAGCGCAC 995 GUGCGCUCCACGUCCUUGG Rh, D [795-813] ORF 24 699 UGUUCUUCAAGCCACACUG 996 CAGUGUGGCUUGAAGAACA Rh, Rb, D [840-858] ORF 25 700 GCCCAAGGGUGUGGUGGAG 997 CUCCACCACACCCUUGGGC Rh, D [1147- 1165] ORF 26 701 ACAGGCCUCUACAACUACU 998 AGUAGUUGUAGAGGCCUGU Rh, Rb, D, Rt, P [947-965] ORF 27 702 UGCGCAGCAGCAAGCAGCA 999 UGCUGCUUGCUGCUGCGCA Rh, D [669-687] ORF 28 703 GGUGGAGGUGACCCAUGAC 1000 GUCAUGGGUCACCUCCACC Rh, Rt, M [1159- 1177] ORF 29 704 CUUUGACCAGGACAUCUAC 1001 GUAGAUGUCCUGGUCAAAG Rh, Rt [1324- 1342] ORF 30 705 AAGGGUGUGGUGGAGGUGA 1002 UCACCUCCACCACACCCUU Rh, D [1151- 1169] ORF 31 706 UCCUAUACCGUGGGUGUCA 1003 UGACACCCACGGUAUAGGA Rh, D, P [914-932] ORF 32 707 GCGCAGACCACCGACGGCA 1004 UGCCGUCGGUGGUCUGCGC D [761-779] ORF 33 708 CGCAGCAGCAAGCAGCACU 1005 AGUGCUGCUUGCUGCUGCG Rh, D [671-689] ORF 34 709 GCCUCAUCAUCCUCAUGCC 1006 GGCAUGAGGAUGAUGAGGC Rh, D, Rt, M [1026- 1044] ORF 35 710 UCUCCAGCCUCAUCAUCCU 1007 AGGAUGAUGAGGCUGGAGA Rh, D, Rt, M [1020- 1038] ORF 36 711 CCAUUGACAAGAACAAGGC 1008 GCCUUGUUCUUGUCAAUGG Rh, D [1215- 1233] ORF 37 712 AGCAGCACUACAACUGCGA 1009 UCGCAGUUGUAGUGCUGCU Rh, D [681-699] ORF 38 713 UGCACCGGACAGGCCUCUA 1010 UAGAGGCCUGUCCGGUGCA Rh, Rb, Rt, P [939-957] ORF 39 714 ACUCCAAGAUCAACUUCCG 1011 CGGAAGUUGAUCUUGGAGU Rh, D, Rt, M [702-720] ORF 40 715 UGGACAACCGUGGCUUCAU 1012 AUGAAGCCACGGUUGUCCA Rh, M [885-903] ORF 41 716 GAGCAGCUGAAGAUCUGGA 1 013 UCCAGAUCUUCAGCUGCUC Rh, D [1091- 1109] ORF 42 717 CAGAAGAAGGCUGUUGCCA 1014 UGGCAACAGCCUUCUUCUG Rt [1121- 1139] ORF 43 718 AGGCAAGAAGGACCUGUAC 1015 GUACAGGUCCUUCUUGCCU Rh, D [1252- 1270] ORF 44 719 CCUCUACAACUACUACGAC 1 016 GUCGUAGUAGUUGUAGAGG Rb, D [952-970] ORF 45 720 AGCAGCUGAAGAUCUGGAU 1017 AUCCAGAUCUUCAGCUGCU Rh, D [1092- 1110] ORF 46 721 AACUACUACGACGACGAGA 1018 UCUCGUCGUCGUAGUAGUU Rb [959-977] ORF 47 722 GGCAAGCUGCCCGAGGUCA 1019 UGACCUCGGGCAGCUUGCC Rh, D [776-794] ORF 48 723 CCGGACAGGCCUCUACAAC 1020 GUUGUAGAGGCCUGUCCGG Rh, Rb, Rt, P [943-961] ORF 49 724 GCUCCCUGCUAUUCAUUGG 1 021 CCAAUGAAUAGCAGGGAGC D [1422- 1440] ORF 50 725 AACUGCGAGCACUCCAAGA 1 022 UCUUGGAGUGCUCGCAGUU Rh, D [692-710] ORF 51 726 GACACAUGGGUGCUAUUGG 1023 CCAAUAGCACCCAUGUGUC Rh, Rt, M [1535- 1553] 3′UTR 52 727 GCACCGGACAGGCCUCUAC 1024 GUAGAGGCCUGUCCGGUGC Rh, Rb, Rt, P [940-958] ORF 53 728 AGCGCAGCGCGCUGCAGUC 1025 GACUGCAGCGCGCUGCGCU Rh, Rt [726-744] ORF 54 729 GGACGUGGAGCGCACGGAC 1026 GUCCGUGCGCUCCACGUCC Rh, D [799-817] ORF 55 730 CAGCCUCAUCAUCCUCAUG 1027 CAUGAGGAUGAUGAGGCUG Rh, D, Rt, M [1024- 1042] ORF 56 731 AAGAUCAACUUCCGCGACA 1028 UGUCGCGGAAGUUGAUCUU D [707-725] ORF 57 732 GCGCAACGUGACCUGGAAG 1029 CUUCCAGGUCACGUUGCGC M [598-616] ORF 58 733 ACUGCGAGCACUCCAAGAU 1030 AUCUUGGAGUGCUCGCAGU Rh, D [693-711] ORF 59 734 GUGGACAACCGUGGCUUCA 1031 UGAAGCCACGGUUGUCCAC Rh, M [884-902] ORF 60 735 CCACAAGCUCUCCAGCCUC 1032 GAGGCUGGAGAGCUUGUGG Rh, D, P [1012- 1030] ORF 61 736 CAAGAUGGUGGACAACCGU 1033 ACGGUUGUCCACCAUCUUG Rh, Rb, M, P [877-895] ORF 62 737 CGAGCACUCCAAGAUCAAC 1034 GUUGAUCUUGGAGUGCUCG Rh, D [697-715] ORF 63 738 AGCUGCCCGAGGUCACCAA 1035 UUGGUGACCUCGGGCAGCU Rh, D [780-798] ORF 64 739 GGACAUCUACGGGCGCGAG 1036 CUCGCGCCCGUAGAUGUCC D [1333- 1351] ORF 65 740 AGGACAUCUACGGGCGCGA 1037 UCGCGCCCGUAGAUGUCCU D [1332- 1350] ORF 66 741 UGUCAGGCAAGAAGGACCU 1038 AGGUCCUUCUUGCCUGACA Rh, D [1248- 1266] ORF 67 742 GGGUGUGGUGGAGGUGACC 1039 GGUCACCUCCACCACACCC Rh, D [1153- 1171] ORF 68 743 CAAGCUCUCCAGCCUCAUC 1040 GAUGAGGCUGGAGAGCUUG Rh, D, M, P [1015- 1033] ORF 69 744 GUGACCCAUGACCUGCAGA 1041 UCUGCAGGUCAUGGGUCAC Rh, Rt, M [1166- 1184] ORF 70 745 GUUCUUCAAGCCACACUGG 1042 CCAGUGUGGCUUGAAGAAC Rh, Rb, D [841-859] ORF 71 746 ACAUCUACGGGCGCGAGGA 1043 UCCUCGCGCCCGUAGAUGU D, M [1335- 1353] ORF 72 747 UGGAGGUGACCCAUGACCU 1044 AGGUCAUGGGUCACCUCCA Rh, Rt, M [1161- 1179] ORF 73 748 UGCAGAAGAAGGCUGUUGC 1045 GCAACAGCCUUCUUCUGCA Rt [1119- 1137] ORF 74 749 UGUACCAGGCCAUGGCCAA 1046 UUGGCCAUGGCCUGGUACA Rh, D [390-408] ORF 75 750 UGUGGUGGAGGUGACCCAU 1047 AUGGGUCACCUCCACCACA Rh, D [1156- 1174] ORF 76 751 AGAAGGACCUGUACCUGGC 1048 GCCAGGUACAGGUCCUUCU Rh, D [1257- 1275] ORF 77 752 AGCAGCUGCGCGACGAGGA 1049 UCCUCGUCGCGCAGCUGCU Rh, D [528-546] ORF 78 753 ACGCCAUGUUCUUCAAGCC 1050 GGCUUGAAGAACAUGGCGU Rh, Rb, P [834-852] ORF 79 754 ACAAGAUGGUGGACAACCG 1051 CGGUUGUCCACCAUCUUGU Rh, Rb, M, P [876-894] ORF 80 755 CUGCGAGCACUCCAAGAUC 1052 GAUCUUGGAGUGCUCGCAG Rh, D [694-712] ORF 81 756 GUCACGCAUGUCAGGCAAG 1053 CUUGCCUGACAUGCGUGAC Rh, D [1240- 1258] ORF 82 757 ACGCAUGUCAGGCAAGAAG 1054 CUUCUUGCCUGACAUGCGU Rh, D [1243- 1261] ORF 83 758 UGCUAUUCAUUGGGCGCCU 1055 AGGCGCCCAAUGAAUAGCA D [1428- 1446] ORF 84 759 UGCGCGACGAGGAGGUGCA 1056 UGCACCUCCUCGUCGCGCA Rh, D [534-552] ORF 85 760 GCAGCUGAAGAUCUGGAUG 1057 CAUCCAGAUCUUCAGCUGC Rh, D [1093- 1111] ORF 86 761 CCAUGACCUGCAGAAACAC 1058 GUGUUUCUGCAGGUCAUGG Rh, Rt, M [1171- 1189] ORF 87 762 AAGCUCUCCAGCCUCAUCA 1059 UGAUGAGGCUGGAGAGCUU Rh, D, Rt, M, P [1016- 1034] ORF 88 763 CAGCAAGCAGCACUACAAC 1060 GUUGUAGUGCUGCUUGCUG Rh, D [676-694] ORF 89 764 AUGUUCUUCAAGCCACACU 1061 AGUGUGGCUUGAAGAACAU Rh, Rb, D [839-857] ORF 90 765 UCCUGAGACACAUGGGUGC 1062 GCACCCAUGUGUCUCAGGA D, Rt, M [1529- 1547] 3′UTR 91 766 CACUCCAAGAUCAACUUCC 1063 GGAAGUUGAUCUUGGAGUG Rh, D, Rt, M [701-719] ORF 92 767 AAGGGUGACAAGAUGCGAG 1064 CUCGCAUCUUGUCACCCUU Rh, D [1457- 1475] ORF 93 768 GACAGGCCUCUACAACUAC 1065 GUAGUUGUAGAGGCCUGUC Rh, Rb, Rt, P [946-964] ORF 94 769 ACCCAUGACCUGCAGAAAC 1066 GUUUCUGCAGGUCAUGGGU Rh, Rt, M [1169- 1187] ORF 95 770 CACCACAAGAUGGUGGACA 1067 UGUCCACCAUCUUGUGGUG Rh, Rb, M, P [872-890] ORF 96 771 GCAGAAGAAGGCUGUUGCC 1068 GGCAACAGCCUUCUUCUGC Rt [1120- 1138] ORF 97 772 GUGGUGGAGGUGACCCAUG 1069 CAUGGGUCACCUCCACCAC Rh, Rb, Rt, M [1157- 1175] ORF 98 773 AGGCCUCUACAACUACUAC 1070 GUAGUAGUUGUAGAGGCCU Rh, Rb, D, Rt, P [949-967] ORF 99 774 GGUGACCCAUGACCUGCAG 1071 CUGCAGGUCAUGGGUCACC Rh, Rt, M [1165- 1183] ORF 100 775 GCCGAGGUGAAGAAACCUG 1072 CAGGUUUCUUCACCUCGGC Rh, Rt [284-302] ORF 101 776 CAACUACUACGACGACGAG 1073 CUCGUCGUCGUAGUAGUUG Rb [958-976] ORF 102 777 CAAGAAGGACCUGUACCUG 1074 CAGGUACAGGUCCUUCUUG Rh, D, M [1255- 1273] ORF 103 778 UGUUCCACGCCACCGCCUU 1075 AAGGCGGUGGCGUGGAACA D [1281- 1299] ORF 104 779 CCCUGCUAUUCAUUGGGCG 1076 CGCCCAAUGAAUAGCAGGG D [1425- 1443] ORF 105 780 CCGUGGCUUCAUGGUGACU 1077 AGUCACCAUGAAGCCACGG Rh, Rt, M [892-910] ORF 106 781 CUACAACUACUACGACGAC 1078 GUCGUCGUAGUAGUUGUAG Rb [955-973] ORF 107 782 GCAGCACUACAACUGCGAG 1079 CUCGCAGUUGUAGUGCUGC Rh, D [682-700] ORF 108 783 UGGUGGACAACCGUGGCUU 1080 AAGCCACGGUUGUCCACCA Rh, M [882-900] ORF 109 784 AGACCACCGACGGCAAGCU 1081 AGCUUGCCGUCGGUGGUCU D, Rt [765-783] ORF 110 785 AGAAACACCUGGCUGGGCU 1082 AGCCCAGCCAGGUGUUUCU D [1182- 1200] ORF 111 786 ACCAAGGACGUGGAGCGCA 1083 UGCGCUCCACGUCCUUGGU Rh, D [794-812] ORF 112 787 CCGAGGUGAAGAAACCUGC 1084 GCAGGUUUCUUCACCUCGG Rh, Rt [285-303] ORF 113 788 ACUACAACUGCGAGCACUC 1085 GAGUGCUCGCAGUUGUAGU Rh, D [687-705] ORF 114 789 ACAAGCUCUCCAGCCUCAU 1086 AUGAGGCUGGAGAGCUUGU Rh, D, M, P [1014- 1032] ORF 115 790 AGGACGUGGAGCGCACGGA 1087 UCCGUGCGCUCCACGUCCU Rh, D [798-816] ORF 116 791 GCUAUUCAUUGGGCGCCUG 1088 CAGGCGCCCAAUGAAUAGC D [1429- 1447] ORF 117 792 AACUUCCGCGACAAGCGCA 1089 UGCGCUUGUCGCGGAAGUU D [713-731] ORF 118 793 GCUCUCCAGCCUCAUCAUC 1090 GAUGAUGAGGCUGGAGAGC Rh, D, Rt, M, P [1018- 1036] ORF 119 794 AGAAGGCUGUUGCCAUCUC 1091 GAGAUGGCAACAGCCUUCU Rt [1125- 1143] ORF 120 795 GGUCACCAAGGACGUGGAG 1092 CUCCACGUCCUUGGUGACC Rh, D [790-808] ORF 121 796 AGCUGCGCGACGAGGAGGU 1093 ACCUCCUCGUCGCGCAGCU Rh, D [531-549] ORF 122 797 CCCGAGGUCACCAAGGACG 1094 CGUCCUUGGUGACCUCGGG Rh, D [785-803] ORF 123 798 AUGUCAGGCAAGAAGGACC 1095 GGUCCUUCUUGCCUGACAU Rh, D [1247- 1265] ORF 124 799 CGAGGUCACCAAGGACGUG 1096 CACGUCCUUGGUGACCUCG Rh, D [787-805] ORF 125 800 GAUGCACCGGACAGGCCUC 1097 GAGGCCUGUCCGGUGCAUC Rh, Rb, Rt, M, P [937-955] ORF 126 801 GCACUACAACUGCGAGCAC 1098 GUGCUCGCAGUUGUAGUGC Rh, D [685-703] ORF 127 802 CCACAAGAUGGUGGACAAC 1099 GUUGUCCACCAUCUUGUGG Rh, Rb, M, P [874-892] ORF 128 803 CAAGGGUGUGGUGGAGGUG 1100 CACCUCCACCACACCCUUG Rh, D [1150- 1168] ORF 129 804 AGCUGAAGAUCUGGAUGGG 1101 CCCAUCCAGAUCUUCAGCU Rh, D [1095- 1113] ORF 130 805 ACCAGGCCAUGGCCAAGGA 1102 UCCUUGGCCAUGGCCUGGU Rh, D [393-411] ORF 131 806 CAUGUUCUUCAAGCCACAC 1103 GUGUGGCUUGAAGAACAUG Rh, Rb, D [838-856] ORF 132 807 CAAGAUCAACUUCCGCGAC 1104 GUCGCGGAAGUUGAUCUUG D [706-724] ORF 133 808 UCCAGCCUCAUCAUCCUCA 1105 UGAGGAUGAUGAGGCUGGA Rh, D, Rt, M [1022- 1040] ORF 134 809 GCCCGAGGUCACCAAGGAC 1106 GUCCUUGGUGACCUCGGGC Rh, D [784-802] ORF 135 810 UCAAGCCACACUGGGAUGA 1107 UCAUCCCAGUGUGGCUUGA Rh, Rb [846-864] ORF 136 811 AGUCCAUCAACGAGUGGGC 1108 GCCCACUCGUUGAUGGACU Rh, Rt, M [741-759] ORF 137 812 GACUUCGUGCGCAGCAGCA 1109 UGCUGCUGCGCACGAAGUC Rh, D, M [662-680] ORF 138 813 CUCUCCAGCCUCAUCAUCC 1110 GGAUGAUGAGGCUGGAGAG Rh, D, Rt, M, P [1019- 1037] ORF 139 814 GCAGACCACCGACGGCAAG 1111 CUUGCCGUCGGUGGUCUGC D, Rt [763-781] ORF 140 815 AUGCAGAAGAAGGCUGUUG 1112 CAACAGCCUUCUUCUGCAU Rt [1118- 1136] ORF 141 816 CAACCGUGGCUUCAUGGUG 1113 CACCAUGAAGCCACGGUUG Rh, Rt, M [889-907] ORF 142 817 UACUACGACGACGAGAAGG 1114 CCUUCUCGUCGUCGUAGUA Rb [962-980] ORF 143 818 GAAGGCUGUUGCCAUCUCC 1115 GGAGAUGGCAACAGCCUUC Rt [1126- 1144] ORF 144 819 UCACCAAGGACGUGGAGCG 1116 CGCUCCACGUCCUUGGUGA Rh, D [792-810] ORF 145 820 CAGCUGAAGAUCUGGAUGG 1117 CCAUCCAGAUCUUCAGCUG Rh, D [1094- 1112] ORF 146 821 UGGGCCUGACUGAGGCCAU 1118 AUGGCCUCAGUCAGGCCCA Rt [1200- 1218] ORF 147 822 ACCGUGGCUUCAUGGUGAC 1119 GUCACCAUGAAGCCACGGU Rh, Rt, M [891-909] ORF 148 823 CAGUCCAUCAACGAGUGGG 1120 CCCACUCGUUGAUGGACUG Rh, Rt, M [740-758] ORF 149 824 CCGACGGCAAGCUGCCCGA 1121 UCGGGCAGCUUGCCGUCGG D [771-789] ORF 150 825 ACAAGCGCAGCGCGCUGCA 1122 UGCAGCGCGCUGCGCUUGU Rh, Rt [723-741] ORF 151 826 GAAACACCUGGCUGGGCUG 1123 CAGCCCAGCCAGGUGUUUC D [1183- 1201] ORF 152 827 AGGCUCCUGAGACACAUGG 1124 CCAUGUGUCUCAGGAGCCU D [1525- 1543] 3′UTR 153 828 CAAGGACGUGGAGCGCACG 1125 CGUGCGCUCCACGUCCUUG Rh, D [796-814] ORF 154 829 GCAGUCCAUCAACGAGUGG 1126 CCACUCGUUGAUGGACUGC Rh, Rt, M [739-757] ORF 155 830 AGAUGGUGGACAACCGUGG 1127 CCACGGUUGUCCACCAUCU Rh, M [879-897] ORF 156 831 AAGCGCAGCGCGCUGCAGU 1128 ACUGCAGCGCGCUGCGCUU Rh, Rt [725-743] ORF 157 832 CAUGUCAGGCAAGAAGGAC 1129 GUCCUUCUUGCCUGACAUG Rh, D [1246- 1264] ORF 158 833 CAAGCCACACUGGGAUGAG 1130 CUCAUCCCAGUGUGGCUUG Rh, Rb [847-865] ORF 159 834 AAGAUGCAGAAGAAGGCUG 1131 CAGCCUUCUUCUGCAUCUU Rh, Rt, M [1115- 1133] ORF 160 835 GGCCAUGGCCAAGGACCAG 1132 CUGGUCCUUGGCCAUGGCC Rh, D [397-415] ORF 161 836 GUGCGCAGCAGCAAGCAGC 1133 GCUGCUUGCUGCUGCGCAC Rh, D [668-686] ORF 162 837 CAACUGCGAGCACUCCAAG 1134 CUUGGAGUGCUCGCAGUUG Rh, D [691-709] ORF 163 838 UACAACUGCGAGCACUCCA 1135 UGGAGUGCUCGCAGUUGUA Rh, D [689-707] ORF 164 839 CAUUGACAAGAACAAGGCC 1136 GGCCUUGUUCUUGUCAAUG Rh, D [1216- 1234] ORF 165 840 CAAGCAGCACUACAACUGC 1137 GCAGUUGUAGUGCUGCUUG Rh, D [679-697] ORF 166 841 GUGUUCCACGCCACCGCCU 1138 AGGCGGUGGCGUGGAACAC D [1280- 1298] ORF 167 842 CCUGCUAUUCAUUGGGCGC 1139 GCGCCCAAUGAAUAGCAGG D [1426- 1444] ORF 168 843 GCCCACAAGCUCUCCAGCC 1140 GGCUGGAGAGCUUGUGGGC Rh, D, P [1010- 1028] ORF 169 844 CAGCAGCAAGCAGCACUAC 1141 GUAGUGCUGCUUGCUGCUG Rh, D [673-691] ORF 170 845 UGAUGCACCGGACAGGCCU 1142 AGGCCUGUCCGGUGCAUCA Rh, Rb, Rt, M, P [936-954] ORF 171 846 UCAACUUCCGCGACAAGCG 1143 CGCUUGUCGCGGAAGUUGA D [711-729] ORF 172 847 UCAGGCAAGAAGGACCUGU 1144 ACAGGUCCUUCUUGCCUGA Rh, D [1250- 1268] ORF 173 848 ACUUCGUGCGCAGCAGCAA 1145 UUGCUGCUGCGCACGAAGU Rh, D, M [663-681] ORF 174 849 ACAACCGUGGCUUCAUGGU 1146 ACCAUGAAGCCACGGUUGU Rh, Rt, M [888-906] ORF 175 850 AAGGCUGUUGCCAUCUCCU 1147 AGGAGAUGGCAACAGCCUU D, Rt [1127- 1145] ORF 176 851 GCAGCUGCGCGACGAGGAG 1148 CUCCUCGUCGCGCAGCUGC Rh, D [529-547] ORF 177 852 UAUUCAUUGGGCGCCUGGU 1149 ACCAGGCGCCCAAUGAAUA D [1431- 1449] ORF 178 853 UCCACCACAAGAUGGUGGA 1150 UCCACCAUCUUGUGGUGGA Rh, Rb, D, P [870-888] ORF 179 854 CCCUGGCCCACAAGCUCUC 1151 GAGAGCUUGUGGGCCAGGG Rh, D, P [1005- 1023] ORF 180 855 ACCAGGACAUCUACGGGCG 1152 CGCCCGUAGAUGUCCUGGU D, Rt [1329- 1347] ORF 181 856 GAUGAUGCACCGGACAGGC 1153 GCCUGUCCGGUGCAUCAUC Rh, Rb, Rt, M [934-952] ORF 182 857 CAACGCCAUGUUCUUCAAG 1154 CUUGAAGAACAUGGCGUUG Rh, Rb, P [832-850] ORF 183 858 ACGGCAAGCUGCCCGAGGU 1155 ACCUCGGGCAGCUUGCCGU Rh, D [774-792] ORF 184 859 CAGCGCGCUGCAGUCCAUC 1156 GAUGGACUGCAGCGCGCUG Rh, Rt [730-748] ORF 185 860 CCCAAGGGUGUGGUGGAGG 1157 CCUCCACCACACCCUUGGG Rh, D [1148- 1166] ORF 186 861 CAUGGCCAAGGACCAGGCA 1158 UGCCUGGUCCUUGGCCAUG Rh, D [400-418] ORF 187 862 CUCCAGCCUCAUCAUCCUC 1159 GAGGAUGAUGAGGCUGGAG Rh, D, Rt, M [1021- 1039] ORF 188 863 UCUACGGGCGCGAGGAGCU 1160 AGCUCCUCGCGCCCGUAGA D, M [1338- 1356] ORF 189 864 GGCCCACAAGCUCUCCAGC 1161 GCUGGAGAGCUUGUGGGCC Rh, D, P [1009- 1027] ORF 190 865 GUCAGGCAAGAAGGACCUG 1162 CAGGUCCUUCUUGCCUGAC Rh, D [1249- 1267] ORF 191 866 CAUCUACGGGCGCGAGGAG 1163 CUCCUCGCGCCCGUAGAUG D, M [1336- 1354] ORF 192 867 CGUGCGCAGCAGCAAGCAG 1164 CUGCUUGCUGCUGCGCACG Rh, D, M [667-685] ORF 193 868 AGCCUCAUCAUCCUCAUGC 1165 GCAUGAGGAUGAUGAGGCU Rh, D, Rt, M [1025- 1043] ORF 194 869 UUCAAGCCACACUGGGAUG 1166 CAUCCCAGUGUGGCUUGAA Rh, Rb [845-863] ORF 195 870 AAGAAGGCUGUUGCCAUCU 1167 AGAUGGCAACAGCCUUCUU Rt [1124- 1142] ORF 196 871 GGUGUGGUGGAGGUGACCC 1168 GGGUCACCUCCACCACACC Rh, D [1154- 1172] ORF 197 872 GAGGUGACCCAUGACCUGC 1169 GCAGGUCAUGGGUCACCUC Rh, Rt, M [1163- 1181] ORF 198 873 GUGGAGGUGACCCAUGACC 1170 GGUCAUGGGUCACCUCCAC Rh, Rt, M [1160- 1178] ORF 199 874 CACAAGAUGGUGGACAACC 1171 GGUUGUCCACCAUCUUGUG Rh, Rb, M, P [875-893] ORF 200 875 CUGGCCCACAAGCUCUCCA 1172 UGGAGAGCUUGUGGGCCAG Rh, D, P [1007- 1025] ORF 201 876 GAUGACUUCGUGCGCAGCA 1173 UGCUGCGCACGAAGUCAUC Rh, Rt, M [659-677] ORF 202 877 ACUUCCGCGACAAGCGCAG 1174 CUGCGCUUGUCGCGGAAGU D [714-732] ORF 203 878 AACGCCAUGUUCUUCAAGC 1175 GCUUGAAGAACAUGGCGUU Rh, Rb, P [833-851] ORF 204 879 GGACCUGUACCUGGCCAGC 1176 GCUGGCCAGGUACAGGUCC Rh, D [1261- 1279] ORF 205 880 GCGACGAGGAGGUGCACGC 1177 GCGUGCACCUCCUCGUCGC D [537-555] ORF 206 881 GCAAGCUGCCCGAGGUCAC 1178 GUGACCUCGGGCAGCUUGC Rh, D [777-795] ORF 207 882 AUUCAUUGGGCGCCUGGUC 1179 GACCAGGCGCCCAAUGAAU D [1432- 1450] ORF 208 883 GAGGUCACCAAGGACGUGG 1180 CCACGUCCUUGGUGACCUC Rh, D [788-806] ORF 209 884 AAGAAGGACCUGUACCUGG 1181 CCAGGUACAGGUCCUUCUU Rh, D [1256- 1274] ORF 210 885 GACAACCGUGGCUUCAUGG 1182 CCAUGAAGCCACGGUUGUC Rh, Rt, M [887-905] ORF 211 886 CUGGGCCUGACUGAGGCCA 1183 UGGCCUCAGUCAGGCCCAG Rt [1199- 1217] ORF 212 887 CUCCAAGAUCAACUUCCGC 1184 GCGGAAGUUGAUCUUGGAG Rh, D, Rt, M [703-721] ORF 213 888 CAACUUCCGCGACAAGCGC 1185 GCGCUUGUCGCGGAAGUUG D [712-730] ORF 214 889 CUCCCUGCUAUUCAUUGGG 1186 CCCAAUGAAUAGCAGGGAG D [1423- 1441] ORF 215 890 AAGCAGCACUACAACUGCG 1187 CGCAGUUGUAGUGCUGCUU Rh, D [680-698] ORF 216 891 GCGCAGCAGCAAGCAGCAC 1188 GUGCUGCUUGCUGCUGCGC Rh, D [670-688] ORF 217 892 CAGGCCAUGGCCAAGGACC 1189 GGUCCUUGGCCAUGGCCUG Rh, D [395-413] ORF 218 893 GUACCAGGCCAUGGCCAAG 1190 CUUGGCCAUGGCCUGGUAC Rh, D [391-409] ORF 219 894 CUUCGUGCGCAGCAGCAAG 1191 CUUGCUGCUGCGCACGAAG Rh, D, M [664-682] ORF 220 895 CAGCACUACAACUGCGAGC 1192 GCUCGCAGUUGUAGUGCUG Rh, D [683-701] ORF 221 896 UACAACUACUACGACGACG 1193 CGUCGUCGUAGUAGUUGUA Rb [956-974] ORF 222 897 GAUGGUGGACAACCGUGGC 1194 GCCACGGUUGUCCACCAUC Rh, M [880-898] ORF 223 898 CUACAACUGCGAGCACUCC 1195 GGAGUGCUCGCAGUUGUAG Rh, D [688-706] ORF 224 899 AAGGACCUGUACCUGGCCA 1196 UGGCCAGGUACAGGUCCUU Rh, D [1259- 1277] ORF 225 900 GCUGCCCGAGGUCACCAAG 1197 CUUGGUGACCUCGGGCAGC Rh, D [781-799] ORF 226 901 GACAUCUACGGGCGCGAGG 1198 CCUCGCGCCCGUAGAUGUC D, M [1334- 1352] ORF 227 902 CCACCACAAGAUGGUGGAC 1199 GUCCACCAUCUUGUGGUGG Rh, Rb, D, P [871-889] ORF 228 903 GCGCGACGAGGAGGUGCAC 1200 GUGCACCUCCUCGUCGCGC Rh, D [535-553] ORF 229 904 CUAUUCAUUGGGCGCCUGG 1201 CCAGGCGCCCAAUGAAUAG D [1430- 1448] ORF 230 905 CCAGGACAUCUACGGGCGC 1202 GCGCCCGUAGAUGUCCUGG D, Rt [1330- 1348] ORF 231 906 AAGAUGGUGGACAACCGUG 1203 CACGGUUGUCCACCAUCUU Rh, M [878-896] ORF 232 907 CAGGACAUCUACGGGCGCG 1204 CGCGCCCGUAGAUGUCCUG D [1331- 1349] ORF 233 908 UCCAAGAUCAACUUCCGCG 1205 CGCGGAAGUUGAUCUUGGA D [704-722] ORF 234 909 GUCACCAAGGACGUGGAGC 1206 GCUCCACGUCCUUGGUGAC Rh, D [791-809] ORF 235 910 CUGCCCGAGGUCACCAAGG 1207 CCUUGGUGACCUCGGGCAG Rh, D [782-800] ORF 236 911 GACCAGGACAUCUACGGGC 1208 GCCCGUAGAUGUCCUGGUC D, Rt [1328- 1346] ORF 237 912 CCAUGGCCAAGGACCAGGC 1209 GCCUGGUCCUUGGCCAUGG Rh, D [399-417] ORF 238 913 CACCAAGGACGUGGAGCGC 1210 GCGCUCCACGUCCUUGGUG Rh, D [793-811] ORF 239 914 GACAAGCGCAGCGCGCUGC 1211 GCAGCGCGCUGCGCUUGUC Rh, Rt [722-740] ORF 240 915 CAAGCGCAGCGCGCUGCAG 1212 CUGCAGCGCGCUGCGCUUG Rh, Rt [724-742] ORF 241 916 CAGACCACCGACGGCAAGC 1213 GCUUGCCGUCGGUGGUCUG D, Rt [764-782] ORF 242 917 GACCACCGACGGCAAGCUG 1214 CAGCUUGCCGUCGGUGGUC D, Rt [766-784] ORF 243 918 AGGACCUGUACCUGGCCAG 1215 CUGGCCAGGUACAGGUCCU Rh, D [1260- 1278] ORF 244 919 CUGCUAUUCAUUGGGCGCC 1216 GGCGCCCAAUGAAUAGCAG D [1427- 1445] ORF 245 920 UCAUUGGGCGCCUGGUCCG 1217 CGGACCAGGCGCCCAAUGA Rh, D [1434- 1452] ORF 246 921 GCUGCGCGACGAGGAGGUG 1218 CACCUCCUCGUCGCGCAGC Rh, D [532-550] ORF 247 922 CGGCAAGCUGCCCGAGGUC 1219 GACCUCGGGCAGCUUGCCG Rh, D [775-793] ORF 248 923 CCUCAUCAUCCUCAUGCCC 1220 GGGCAUGAGGAUGAUGAGG Rh, D, Rt, M [1027- 1045] ORF 249 924 CCAGGCCAUGGCCAAGGAC 1221 GUCCUUGGCCAUGGCCUGG Rh, D [394-412] ORF 250 925 GCCAUGGCCAAGGACCAGG 1222 CCUGGUCCUUGGCCAUGGC Rh, D [398-416] ORF 251 926 CCACCGACGGCAAGCUGCC 1223 GGCAGCUUGCCGUCGGUGG D, Rt [768-786] ORF 252 927 AUGGUGGACAACCGUGGCU 1224 AGCCACGGUUGUCCACCAU Rh, M [881-899] ORF 253 928 CUUCCGCGACAAGCGCAGC 1225 GCUGCGCUUGUCGCGGAAG D [715-733] ORF 254 929 CGCGACGAGGAGGUGCACG 1226 CGUGCACCUCCUCGUCGCG Rh, D [536-554] ORF 255 930 UGGCCCACAAGCUCUCCAG 1227 CUGGAGAGCUUGUGGGCCA Rh, D, P [1008- 1026] ORF 256 931 GAGCAGCUGCGCGACGAGG 1228 CCUCGUCGCGCAGCUGCUC Rh, D [527-545] ORF 257 932 UGACCAGGACAUCUACGGG 1229 CCCGUAGAUGUCCUGGUCA Rt [1327- 1345] ORF 258 933 ACCACCGACGGCAAGCUGC 1230 GCAGCUUGCCGUCGGUGGU D, Rt [767-785] ORF 259 934 GAAGGACCUGUACCUGGCC 1231 GGCCAGGUACAGGUCCUUC Rh, D [1258- 1276] ORF 260 935 CAUUGGGCGCCUGGUCCGG 1232 CCGGACCAGGCGCCCAAUG Rh, D [1435- 1453] ORF 261 936 AUGCACCGGACAGGCCUCU 1233 AGAGGCCUGUCCGGUGCAU Rh, Rb, Rt, P [938-956] ORF 262 937 AUCAACUUCCGCGACAAGC 1234 GCUUGUCGCGGAAGUUGAU D [710-728] ORF 263 938 CAGCUGCGCGACGAGGAGG 1235 CCUCCUCGUCGCGCAGCUG Rh, D [530-548] ORF 264 939 CAGAAACACCUGGCUGGGC 1236 GCCCAGCCAGGUGUUUCUG D [1181- 1199] ORF 265 940 CUACGGGCGCGAGGAGCUG 1237 CAGCUCCUCGCGCCCGUAG D, M [1339- 1357] ORF 266 941 CGACGAGGAGGUGCACGCC 1238 GGCGUGCACCUCCUCGUCG D [538-556] ORF 267 942 UUUGACCAGGACAUCUACG 1239 CGUAGAUGUCCUGGUCAAA Rt [1325- 1343] ORF 268 943 GUCCAUCAACGAGUGGGCC 1240 GGCCCACUCGUUGAUGGAC Rh, Rt, M [742-760] ORF 269 944 AUGACUUCGUGCGCAGCAG 1241 CUGCUGCGCACGAAGUCAU Rh, Rt, M [660-678] ORF 270 945 UCCCUGCUAUUCAUUGGGC 1242 GCCCAAUGAAUAGCAGGGA D [1424- 1442] ORF 271 946 CUGCGCGACGAGGAGGUGC 1243 GCACCUCCUCGUCGCGCAG Rh, D [533-551] ORF 272 947 CAAGCUGCCCGAGGUCACC 1244 GGUGACCUCGGGCAGCUUG Rh, D [778-796] ORF 273 948 AAGCUGCCCGAGGUCACCA 1245 UGGUGACCUCGGGCAGCUU Rh, D [779-797] ORF 274 949 UUCUUCAAGCCACACUGGG 1246 CCCAGUGUGGCUUGAAGAA Rh, Rb, D [842-860] ORF 275 950 ACACCUGGCUGGGCUGGGC 1247 GCCCAGCCCAGCCAGGUGU D [1186- 1204] ORF 276 951 UCCAUCAACGAGUGGGCCG 1248 CGGCCCACUCGUUGAUGGA Rt, M [743-761] ORF 277 952 AUCUACGGGCGCGAGGAGC 1249 GCUCCUCGCGCCCGUAGAU D, M [1337- 1355] ORF 278 953 UCGUGCGCAGCAGCAAGCA 1250 UGCUUGCUGCUGCGCACGA Rh, D, M [666-684] ORF 279 954 CGACGGCAAGCUGCCCGAG 1251 CUCGGGCAGCUUGCCGUCG D [772-790] ORF 280 955 UUCAUUGGGCGCCUGGUCC 1252 GGACCAGGCGCCCAAUGAA Rh, D [1433- 1451] ORF 281 956 UUGACCAGGACAUCUACGG 1253 CCGUAGAUGUCCUGGUCAA Rt [1326- 1344] ORF 282 957 CCUGGCCCACAAGCUCUCC 1254 GGAGAGCUUGUGGGCCAGG Rh, D, P [1006- 1024] ORF 283 958 UGACUUCGUGCGCAGCAGC 1255 GCUGCUGCGCACGAAGUCA Rh, Rt, M [661-679] ORF 284 959 AUGAUGCACCGGACAGGCC 1256 GGCCUGUCCGGUGCAUCAU Rh, Rb, Rt, M, P [935-953] ORF 285 960 CACCGACGGCAAGCUGCCC 1257 GGGCAGCUUGCCGUCGGUG D, Rt [769-787] ORF 286 961 GACGGCAAGCUGCCCGAGG 1258 CCUCGGGCAGCUUGCCGUC Rh, D [773-791] ORF 287 962 UACCAGGCCAUGGCCAAGG 1259 CCUUGGCCAUGGCCUGGUA Rh, D [392-410] ORF 288 963 UCCGCGACAAGCGCAGCGC 1260 GCGCUGCGCUUGUCGCGGA D [717-735] ORF 289 964 UUCCGCGACAAGCGCAGCG 1261 CGCUGCGCUUGUCGCGGAA D [716-734] ORF 290 965 AAGGACGUGGAGCGCACGG 1262 CCGUGCGCUCCACGUCCUU Rh, D [797-815] ORF 291 966 UUCCACCACAAGAUGGUGG 1263 CCACCAUCUUGUGGUGGAA Rh, Rb, D, P [869-887] ORF 292 967 UACGGGCGCGAGGAGCUGC 1264 GCAGCUCCUCGCGCCCGUA D, M [1340- 1358] ORF 293 968 AAACACCUGGCUGGGCUGG 1265 CCAGCCCAGCCAGGUGUUU D [1184- 1202] ORF 294 969 AACACCUGGCUGGGCUGGG 1266 CCCAGCCCAGCCAGGUGUU D [1185- 1203] ORF 295 970 AUUGGGCGCCUGGUCCGGC 1267 GCCGGACCAGGCGCCCAAU Rh, D [1436- 1454] ORF 296 971 ACCGACGGCAAGCUGCCCG 1268 CGGGCAGCUUGCCGUCGGU D [770-788] ORF 297 972 UUCGUGCGCAGCAGCAAGC 1269 GCUUGCUGCUGCGCACGAA Rh, D, M [665-683] ORF

TABLE D SERPINH1 Active 18 + 1-mer siRNAs human- 32454740 ORF: 230- No. SEQ ID Sense siRNA SEQ ID AntiSense siRNA Other Sp 1486 1 1270 AGCCUUUGUUGCUAUCAAA 1849 UUUGAUAGCAACAAAGGCU Rh [2117-2135] 3′UTR 2 1271 GCCUAAGGGUGACAAGAUA 1850 UAUCUUGUCACCCUUAGGC Rh [1453-1471] ORF 3 1272 GGCCUAAGGGUGACAAGAA 1851 UUCUUGUCACCCUUAGGCC Rh [1452-1470] ORF 4 1273 CCUCAAUCAGUAUUCAUAA 1852 UUAUGAAUACUGAUUGAG [1774-1792] G 3′UTR 5 1274 GGCGGAUUGAGAAGGAGCA 1853 UGCUCCUUCUCAAUCCGCC [1973-1991] 3′UTR 6 1275 GGCAGUGGAGAACAUCCUA 1854 UAGGAUGUUCUCCACUGCC Rh [415-433] ORF 7 1276 GGGUCAGCCAGCCCUCUUA 1855 UAAGAGGGCUGGCUGACCC Rh [1839-1857] 3′UTR 8 1277 GGGUGACAAGAUGCGAGAA 1856 UUCUCGCAUCUUGUCACCC Rh, D [1459-1477] ORF 9 1278 GGACCAGGCAGUGGAGAAA 1857 UUUCUCCACUGCCUGGUCC Rh [409-427] ORF 10 1279 GAGACACAUGGGUGCUAUA 1858 UAUAGCACCCAUGUGUCUC Rh, D, Rt, [1533-1551] M 3′UTR 11 1280 GUUGGAGCGUGGAAAAAAA 1859 UUUUUUUCCACGCUCCAAC [2191-2208] 3′UTR 12 1281 GGAACAUGAGCCUUUGUUA 1860 UAACAAAGGCUCAUGUUCC Rh [2109-2127] 3′UTR 13 1282 GCCAUGUUCUUCAAGCCAA 1861 UUGGCUUGAAGAACAUGGC Rh, Rb, D [836-854] ORF 14 1283 GGAUUGAGAAGGAGCUCCA 1862 UGGAGCUCCUUCUCAAUCC [1976-1994] 3′UTR 15 1284 GGGAUGAACUUUUUGUUUA 1863 UAAACAAAAAGUUCAUCCC Rh [2048-2066] 3′UTR 16 1285 GCCGCAGUGAGGCGGAUUA 1864 UAAUCCGCCUCACUGCGGC [1963-1981] 3′UTR 17 1286 GGACCUUCCCAGCUAGAAA 1865 UUUCUAGCUGGGAAGGUCC Rh [1639-1657] 3′UTR 18 1287 GACCUUCCCAGCUAGAAUA 1866 UAUUCUAGCUGGGAAGGUC Rh [1640-1658] 3′UTR 19 1288 CCUGUGAGACCAAAUUGAA 1867 UUCAAUUUGGUCUCACAGG Rh [1814-1832] 3′UTR 20 1289 UGGAGAACAUCCUGGUGUA 1868 UACACCAGGAUGUUCUCCA Rh [420-438] ORF 21 1290 GCCUUUGUUGCUAUCAAUA 1869 UAUUGAUAGCAACAAAGGC Rh [2118-2136] 3′UTR 22 1291 CCGCCUUUGAGUUGGACAA 1870 UUGUCCAACUCAAAGGCGG Rh [1293-1311] ORF 23 1292 CAGGCAGUGGAGAACAUCA 1871 UGAUGUUCUCCACUGCCUG Rh [413-431] ORF 24 1293 CACCUGUGAGACCAAAUUA 1872 UAAUUUGGUCUCACAGGUG Rh [1812-1830] 3′UTR 25 1294 GGGAAGAUGCAGAAGAAGA 1873 UCUUCUUCUGCAUCUUCCC Rh, Rb, Rt [1112-1130] ORF 26 1295 GGCCAUUGACAAGAACAAA 1874 UUUGUUCUUGUCAAUGGCC Rh, D [1213-1231] ORF 27 1296 GCCUUUGAGUUGGACACAA 1875 UUGUGUCCAACUCAAAGGC Rh [1295-1313] ORF 28 1297 AGCGGACCUUCCCAGCUAA 1876 UUAGCUGGGAAGGUCCGCU Rh [1636-1654] 3′UTR 29 1298 GAAGAAGGCUGUUGCCAUA 1877 UAUGGCAACAGCCUUCUUC Rt [1123-1141] ORF 30 1299 ACAAGAUGCGAGACGAGUA 1878 UACUCGUCUCGCAUCUUGU Rh, Rt [1464-1482] ORF 31 1300 GAGGCGGAUUGAGAAGGAA 1879 UUCCUUCUCAAUCCGCCUC [1971-1989] 3′UTR 32 1301 GGACAACCGUGGCUUCAUA 1880 UAUGAAGCCACGGUUGUCC Rh, M [886-904] ORF 33 1302 CAUAUUUAUAGCCAGGUAA 1881 UUACCUGGCUAUAAAUAUG Rh [1788-1806] 3′UTR 34 1303 CGACGACGAGAAGGAAAAA 1882 UUUUUCCUUCUCGUCGUCG [967-985] ORF 35 1304 CUCACCUGUGAGACCAAAA 1883 UUUUGGUCUCACAGGUGAG Rh [1810-1828] 3′UTR 36 1305 GCGGCUCCCUGCUAUUCAA 1884 UUGAAUAGCAGGGAGCCGC [1419-1437] ORF 37 1306 AGAACAUCCUGGUGUCACA 1885 UGUGACACCAGGAUGUUCU [423-441] ORF 38 1307 CACACUGGGAUGAGAAAUA 1886 UAUUUCUCAUCCCAGUGUG Rh [852-870] ORF 39 1308 GCUAGAAUUCACUCCACUA 1887 UAGUGGAGUGAAUUCUAGC Rh [1650-1668] 3′UTR 40 1309 CCUUCAUCUUCCUAGUGCA 1888 UGCACUAGGAAGAUGAAGG [1389-1407] ORF 41 1310 UGCUAUCAAUCCAAGAACA 1889 UGUUCUUGGAUUGAUAGCA Rh [2126-2144] 3′UTR 42 1311 GGAAGAUGCAGAAGAAGGA 1890 UCCUUCUUCUGCAUCUUCC Rh, Rb, Rt [1113-1131] ORF 43 1312 CAUGAGCCUUUGUUGCUAA 1891 UUAGCAACAAAGGCUCAUG Rh [2113-2131] 3′UTR 44 1313 GCGGAUUGAGAAGGAGCUA 1892 UAGCUCCUUCUCAAUCCGC [1974-1992] 3′UTR 45 1314 UGCAGUCCAUCAACGAGUA 1893 UACUCGUUGAUGGACUGCA Rh, Rt, M [738-756] ORF 46 1315 GCACUGCGGAGAAGUUGAA 1894 UUCAACUUCUCCGCAGUGC [321-339] ORF 47 1316 CCAGGCAGUGGAGAACAUA 1895 UAUGUUCUCCACUGCCUGG Rh [412-430] ORF 48 1317 GGCAAGAAGGACCUGUACA 1896 UGUACAGGUCCUUCUUGCC Rh, D, M [1253-1271] ORF 49 1318 CUCUACAACUACUACGACA 1897 UGUCGUAGUAGUUGUAGA Rb [953-971] G ORF 50 1319 CUUCCCAGCUAGAAUUCAA 1898 UUGAAUUCUAGCUGGGAAG Rh [1643-1661] 3′UTR 51 1320 AGGCGGAUUGAGAAGGAGA 1899 UCUCCUUCUCAAUCCGCCU [1972-1990] 3′UTR 52 1321 GGUCCUAUACCGUGGGUGA 1900 UCACCCACGGUAUAGGACC Rh [912-930] ORF 53 1322 GCAAGAAGGACCUGUACCA 1901 UGGUACAGGUCCUUCUUGC Rh, D, M [1254-1272] ORF 54 1323 CCGUGGGUGUCAUGAUGAA 1902 UUCAUCAUGACACCCACGG Rh [921-939] ORF 55 1324 GAUGCGAGACGAGUUAUAA 1903 UUAUAACUCGUCUCGCAUC Rh [1468-1486] ORF 56 1325 GGCAGUGCUGAGCGCCGAA 1904 UUCGGCGCUCAGCACUGCC [511-529] ORF 57 1326 CAGCUAGAAUUCACUCCAA 1905 UUGGAGUGAAUUCUAGCUG Rh [1648-1666] 3′UTR 58 1327 GAGCUUCGCUGAUGACUUA 1906 UAAGUCAUCAGCGAAGCUC Rh [649-667] ORF 59 1328 CUUUGAGUUGGACACAGAA 1907 UUCUGUGUCCAACUCAAAG Rh [1297-1315] ORF 60 1329 GGUGGACAACCGUGGCUUA 1908 UAAGCCACGGUUGUCCACC Rh, M [883-901] ORF 61 1330 GCCUCAUCAUCCUCAUGCA 1909 UGCAUGAGGAUGAUGAGGC Rh, D, Rt, [1026-1044] M ORF 62 1331 ACCAGGCAGUGGAGAACAA 1910 UUGUUCUCCACUGCCUGGU Rh [411-429] ORF 63 1332 CCUGCCUCAAUCAGUAUUA 1911 UAAUACUGAUUGAGGCAGG [1770-1788] 3′UTR 64 1333 GAUCAAGCCUGCCUCAAUA 1912 UAUUGAGGCAGGCUUGAUC Rh [1763-1781] 3′UTR 65 1334 CAGACUCUGGUCAAGAAGA 1913 UCUUCUUGACCAGAGUCUG Rh [2011-2029] 3′UTR 66 1335 CGCGCUGCAGUCCAUCAAA 1914 UUUGAUGGACUGCAGCGCG Rh, Rt [733-751] ORF 67 1336 CUGGCACUGCGGAGAAGUA 1915 UACUUCUCCGCAGUGCCAG [318-336] ORF 68 1337 CCAGCUCUAUCCCAACCUA 1916 UAGGUUGGGAUAGAGCUG [1885-1903] G 3′UTR 69 1338 AGGGUGUGGUGGAGGUGAA 1917 UUCACCUCCACCACACCCU Rh, D [1152-1170] ORF 70 1339 AGUGAGGCGGAUUGAGAAA 1918 UUUCUCAAUCCGCCUCACU [1968-1986] 3′UTR 71 1340 CGGACAGGCCUCUACAACA 1919 UGUUGUAGAGGCCUGUCCG Rh, Rb, Rt, [944-962] P ORF 72 1341 CGACGAGAAGGAAAAGCUA 1920 UAGCUUUUCCUUCUCGUCG Rh [970-988] ORF 73 1342 AGGCCAAGGCAGUGCUGAA 1921 UUCAGCACUGCCUUGGCCU Rh [504-522] ORF 74 1343 GCCUCAGGGUGCACACAGA 1922 UCUGUGUGCACCCUGAGGC [1488-1506] 3′UTR 75 1344 GGAUGAGAAAUUCCACCAA 1923 UUGGUGGAAUUUCUCAUCC Rh [859-877] ORF 76 1345 AGAAGGAAAAGCUGCAAAA 1924 UUUUGCAGCUUUUCCUUCU Rh [975-993] ORF 77 1346 AGCUCUAUCCCAACCUCUA 1925 UAGAGGUUGGGAUAGAGC Rh [1887-1905] U 3′UTR 78 1347 UGACAAGAUGCGAGACGAA 1926 UUCGUCUCGCAUCUUGUCA Rh [1462-1480] ORF 79 1348 AGAAGGAGCUCCCAGGAGA 1927 UCUCCUGGGAGCUCCUUCU [1982-2000] 3′UTR 80 1349 CCUUCUCACCUGUGAGACA 1928 UGUCUCACAGGUGAGAAGG Rh [1806-1824] 3′UTR 81 1350 GGCUUCUGGGCAGACUCUA 1929 UAGAGUCUGCCCAGAAGCC Rh [2001-2019] 3′UTR 82 1351 CCAGCCUCAUCAUCCUCAA 1930 UUGAGGAUGAUGAGGCUG Rh, D, Rt, [1023-1041] G M ORF 83 1352 CCAAAGGCUCCUGAGACAA 1931 UUGUCUCAGGAGCCUUUGG [1521-1539] 3′UTR 84 1353 GGACCUGGGCCAUAGUCAA 1932 UUGACUAUGGCCCAGGUCC [1722-1740] 3′UTR 85 1354 GGGUGUCAUGAUGAUGCAA 1933 UUGCAUCAUCAUGACACCC Rh [925-943] ORF 86 1355 GUACCAGCCUUGGAUACUA 1934 UAGUAUCCAAGGCUGGUAC Rh [1572-1590] 3′UTR 87 1356 GGCUGUUGCCAUCUCCUUA 1935 UAAGGAGAUGGCAACAGCC [1129-1147] ORF 88 1357 CGCAGUGAGGCGGAUUGAA 1936 UUCAAUCCGCCUCACUGCG [1965-1983] 3′UTR 89 1358 CCAAGGACGUGGAGCGCAA 1937 UUGCGCUCCACGUCCUUGG Rh, D [795-813] ORF 90 1359 GGCUCCUGAGACACAUGGA 1938 UCCAUGUGUCUCAGGAGCC D [1526-1544] 3′UTR 91 1360 GCUGCAGUCCAUCAACGAA 1939 UUCGUUGAUGGACUGCAGC Rh, Rt [736-754] ORF 92 1361 CCAGGUACCUUCUCACCUA 1940 UAGGUGAGAAGGUACCUGG Rh [1799-1817] 3′UTR 93 1362 GCAGCGCGCUGCAGUCCAA 1941 UUGGACUGCAGCGCGCUGC Rh, Rt [729-747] ORF 94 1363 GAGACCAAAUUGAGCUAGA 1942 UCUAGCUCAAUUUGGUCUC Rh [1819-1837] 3′UTR 95 1364 GCCGCCGAGGUGAAGAAAA 1943 UUUUCUUCACCUCGGCGGC [281-299] ORF 96 1365 GCAGACUCUGGUCAAGAAA 1944 UUUCUUGACCAGAGUCUGC Rh [2010-2028] 3′UTR 97 1366 CUAGAAUUCACUCCACUUA 1945 UAAGUGGAGUGAAUUCUA Rh [1651-1669] G 3′UTR 98 1367 GCAGUGGAGAACAUCCUGA 1946 UCAGGAUGUUCUCCACUGC Rh [416-434] ORF 99 1368 CGCAUGUCAGGCAAGAAGA 1947 UCUUCUUGCCUGACAUGCG Rh, D [1244-1262] ORF 100 1369 CGGAUUGAGAAGGAGCUCA 1948 UGAGCUCCUUCUCAAUCCG [1975-1993] 3′UTR 101 1370 AGGUGAGGUACCAGCCUUA 1949 UAAGGCUGGUACCUCACCU Rh [1565-1583] 3′UTR 102 1371 CCACACUGGGAUGAGAAAA 1950 UUUUCUCAUCCCAGUGUGG Rh [851-869] ORF 103 1372 GCCAUUGACAAGAACAAGA 1951 UCUUGUUCUUGUCAAUGGC Rh, D [1214-1232] ORF 104 1373 GCGCUGCAGUCCAUCAACA 1952 UGUUGAUGGACUGCAGCGC Rh, Rt [734-752] ORF 105 1374 CUCCCAACUAUAAAACUAA 1953 UUAGUUUUAUAGUUGGGA Rh [1903-1921] G 3′UTR 106 1375 GGUGACAAGAUGCGAGACA 1954 UGUCUCGCAUCUUGUCACC Rh [1460-1478] ORF 107 1376 GGCCGACUUGUCACGCAUA 1955 UAUGCGUGACAAGUCGGCC Rh [1231-1249] ORF 108 1377 CCUAAGGGUGACAAGAUGA 1956 UCAUCUUGUCACCCUUAGG Rh [1454-1472] ORF 109 1378 UGAGACACAUGGGUGCUAA 1957 UUAGCACCCAUGUGUCUCA Rh, D, Rt, [1532-1550] M 3′UTR 110 1379 GGGUGGAAAAACAGACCGA 1958 UCGGUCUGUUUUUCCACCC [1601-1619] 3′UTR 111 1380 GGUGGAGGUGACCCAUGAA 1959 UUCAUGGGUCACCUCCACC Rh, Rt, M [1159-1177] ORF 112 1381 CUUUGACCAGGACAUCUAA 1960 UUAGAUGUCCUGGUCAAAG Rh, Rt [1324-1342] ORF 113 1382 GAACAUGAGCCUUUGUUGA 1961 UCAACAAAGGCUCAUGUUC Rh [2110-2128] 3′UTR 114 1383 AGCCUUGGAUACUCCAUGA 1962 UCAUGGAGUAUCCAAGGCU Rh [1577-1595] 3′UTR 115 1384 GGAGGUGACCCAUGACCUA 1963 UAGGUCAUGGGUCACCUCC Rh, Rt, M [1162-1180] ORF 116 1385 AGAUCAAGCCUGCCUCAAA 1964 UUUGAGGCAGGCUUGAUCU Rh [1762-1780] 3′UTR 117 1386 GCCCAAGGGUGUGGUGGAA 1965 UUCCACCACACCCUUGGGC Rh, D [1147-1165] ORF 118 1387 AGAACAAGGCCGACUUGUA 1966 UACAAGUCGGCCUUGUUCU Rh [1224-1242] ORF 119 1388 GUGGCUUCAUGGUGACUCA 1967 UGAGUCACCAUGAAGCCAC Rh [894-912] ORF 120 1389 CUCCUGAGACACAUGGGUA 1968 UACCCAUGUGUCUCAGGAG D [1528-1546] 3′UTR 121 1390 CAGCCUUGGAUACUCCAUA 1969 UAUGGAGUAUCCAAGGCUG Rh [1576-1594] 3′UTR 122 1391 AAGGCUCCUGAGACACAUA 1970 UAUGUGUCUCAGGAGCCUU D [1524-1542] 3′UTR 123 1392 AGAAGAAGGCUGUUGCCAA 1971 UUGGCAACAGCCUUCUUCU Rt [1122-1140] ORF 124 1393 CUACUACGACGACGAGAAA 1972 UUUCUCGUCGUCGUAGUAG Rb [961-979] ORF 125 1394 CCUUUGUUGCUAUCAAUCA 1973 UGAUUGAUAGCAACAAAGG Rh [2119-2137] 3′UTR 126 1395 AGGCAGUGGAGAACAUCCA 1974 UGGAUGUUCUCCACUGCCU Rh [414-432] ORF 127 1396 CCAUCACGUGGAGCCUCUA 1975 UAGAGGCUCCACGUGAUGG Rh [1045-1063] ORF 128 1397 AGCUCUCCAGCCUCAUCAA 1976 UUGAUGAGGCUGGAGAGCU Rh, D, Rt, [1017-1035] M, P ORF 129 1398 GGCUCCCUGCUAUUCAUUA 1977 UAAUGAAUAGCAGGGAGCC D [1421-1439] ORF 130 1399 GGGAACAUGAGCCUUUGUA 1978 UACAAAGGCUCAUGUUCCC Rh [2108-2126] 3′UTR 131 1400 GGGCCAUAGUCAUUCUGCA 1979 UGCAGAAUGACUAUGGCCC [1728-1746] 3′UTR 132 1401 CCAAAGAGCAGCUGAAGAA 1980 UUCUUCAGCUGCUCUUUGG Rh, Rb, P [1086-1104] ORF 133 1402 GACGAGAAGGAAAAGCUGA 1981 UCAGCUUUUCCUUCUCGUC Rh [971-989] ORF 134 1403 GGGCUUCUGGGCAGACUCA 1982 UGAGUCUGCCCAGAAGCCC Rh [2000-2018] 3′UTR 135 1404 CAAGGACCAGGCAGUGGAA 1983 UUCCACUGCCUGGUCCUUG Rh [406-424] ORF 136 1405 CUGUGAGACCAAAUUGAGA 1984 UCUCAAUUUGGUCUCACAG Rh [1815-1833] 3′UTR 137 1406 GACUGAGGCCAUUGACAAA 1985 UUUGUCAAUGGCCUCAGUC Rh [1207-1225] ORF 138 1407 GACUUGUCACGCAUGUCAA 1986 UUGACAUGCGUGACAAGUC Rh [1235-1253] ORF 139 1408 GAGGUGAGGUACCAGCCUA 1987 UAGGCUGGUACCUCACCUC [1564-1582] 3′UTR 140 1409 CAGAUACCAUGAUGCUGAA 1988 UUCAGCAUCAUGGUAUCUG Rh [1681-1699] 3′UTR 141 1410 AGGCAAGAAGGACCUGUAA 1989 UUACAGGUCCUUCUUGCCU Rh, D [1252-1270] ORF 142 1411 CUGGGAUGAGAAAUUCCAA 1990 UUGGAAUUUCUCAUCCCAG Rh [856-874] ORF 143 1412 AGGUACCAGCCUUGGAUAA 1991 UUAUCCAAGGCUGGUACCU Rh [1570-1588] 3′UTR 144 1413 CAGCCAGCCCUCUUCUGAA 1992 UUCAGAAGAGGGCUGGCUG [1843-1861] 3′UTR 145 1414 GUGUCAUGAUGAUGCACCA 1993 UGGUGCAUCAUCAUGACAC Rh [927-945] ORF 146 1415 CCUCUACAACUACUACGAA 1994 UUCGUAGUAGUUGUAGAG Rb, D [952-970] G ORF 147 1416 CCGCCGAGGUGAAGAAACA 1995 UGUUUCUUCACCUCGGCGG Rh [282-300] ORF 148 1417 GCUAUCAAUCCAAGAACUA 1996 UAGUUCUUGGAUUGAUAGC Rh [2127-2145] 3′UTR 149 1418 AGCCUGCCUCAAUCAGUAA 1997 UUACUGAUUGAGGCAGGCU [1768-1786] 3′UTR 150 1419 GGUCCGGCCUAAGGGUGAA 1998 UUCACCCUUAGGCCGGACC Rh [1447-1465] ORF 151 1420 GAAGGAAAAGCUGCAAAUA 1999 UAUUUGCAGCUUUUCCUUC Rh [976-994] ORF 152 1421 GGCCUCUACAACUACUACA 2000 UGUAGUAGUUGUAGAGGCC Rb, D [950-968] ORF 153 1422 UGUUCUUCAAGCCACACUA 2001 UAGUGUGGCUUGAAGAACA Rh, Rb, D [840-858] ORF 154 1423 GGCCAAGGCAGUGCUGAGA 2002 UCUCAGCACUGCCUUGGCC Rh [505-523] ORF 155 1424 AGAAAUUCCACCACAAGAA 2003 UUCUUGUGGUGGAAUUUCU Rh [864-882] ORF 156 1425 CUGCAGUCCAUCAACGAGA 2004 UCUCGUUGAUGGACUGCAG Rh, Rt, M [737-755] ORF 157 1426 CCAGCGUGUUCCACGCCAA 2005 UUGGCGUGGAACACGCUGG [1275-1293] ORF 158 1427 GCUCCCUCCUGCUUCUCAA 2006 UUGAGAAGCAGGAGGGAGC [234-252] ORF 159 1428 CCGGACAGGCCUCUACAAA 2007 UUUGUAGAGGCCUGUCCGG Rh, Rb, Rt, [943-961] P ORF 160 1429 CCCAUCACGUGGAGCCUCA 2008 UGAGGCUCCACGUGAUGGG Rh [1044-1062] ORF 161 1430 CCGGCCUAAGGGUGACAAA 2009 UUUGUCACCCUUAGGCCGG Rh [1450-1468] ORF 162 1431 CCUAUACCGUGGGUGUCAA 2010 UUGACACCCACGGUAUAGG Rh, D, P [915-933] ORF 163 1432 CAGUGGAGAACAUCCUGGA 2011 UCCAGGAUGUUCUCCACUG Rh [417-435] ORF 164 1433 CACUGGGAUGAGAAAUUCA 2012 UGAAUUUCUCAUCCCAGUG Rh [854-872] ORF 165 1434 AUCCAAAGGCUCCUGAGAA 2013 UUCUCAGGAGCCUUUGGAU [1519-1537] 3′UTR 166 1435 UGAGAAAUUCCACCACAAA 2014 UUUGUGGUGGAAUUUCUCA Rh [862-880] ORF 167 1436 GGUGGAAAAACAGACCGGA 2015 UCCGGUCUGUUUUUCCACC [1602-1620] 3′UTR 168 1437 GCUGGGCAGCCGACUGUAA 2016 UUACAGUCGGCUGCCCAGC [616-634] ORF 169 1438 CCAUAGUCAUUCUGCCUGA 2017 UCAGGCAGAAUGACUAUGG [1731-1749] 3′UTR 170 1439 GCACCGGACAGGCCUCUAA 2018 UUAGAGGCCUGUCCGGUGC Rh, Rb, Rt, [940-958] P ORF 171 1440 GUUGGACACAGAUGGCAAA 2019 UUUGCCAUCUGUGUCCAAC [1303-1321] ORF 172 1441 GCCUGCCUCAAUCAGUAUA 2020 UAUACUGAUUGAGGCAGGC [1769-1787] 3′UTR 173 1442 GAUCAACUUCCGCGACAAA 2021 UUUGUCGCGGAAGUUGAUC D [709-727] ORF 174 1443 GGCCGCAGUGAGGCGGAUA 2022 UAUCCGCCUCACUGCGGCC [1962-1980] 3′UTR 175 1444 CUGCGGAGAAGUUGAGCCA 2023 UGGCUCAACUUCUCCGCAG [324-342] ORF 176 1445 GCAUCCAAAGGCUCCUGAA 2024 UUCAGGAGCCUUUGGAUGC [1517-1535] 3′UTR 177 1446 GCUUCUGGGCAGACUCUGA 2025 UCAGAGUCUGCCCAGAAGC Rh [2002-2020] 3′UTR 178 1447 CCAGCCCUCUUCUGACACA 2026 UGUGUCAGAAGAGGGCUGG [1846-1864] 3′UTR 179 1448 GCUCUAUCCCAACCUCUCA 2027 UGAGAGGUUGGGAUAGAG Rh [1888-1906] C 3′UTR 180 1449 GGACGUGGAGCGCACGGAA 2028 UUCCGUGCGCUCCACGUCC Rh, D [799-817] ORF 181 1450 CCAAGGCAGUGCUGAGCGA 2029 UCGCUCAGCACUGCCUUGG Rh [507-525] ORF 182 1451 GCAGAAGAAGGCUGUUGCA 2030 UGCAACAGCCUUCUUCUGC Rt [1120-1138] ORF 183 1452 GACAUUUUGUUGGAGCGUA 2031 UACGCUCCAACAAAAUGUC [2183-2201] 3′UTR 184 1453 CGAGCACUCCAAGAUCAAA 2032 UUUGAUCUUGGAGUGCUCG Rh, D [697-715] ORF 185 1454 UCAUGAUGAUGCACCGGAA 2033 UUCCGGUGCAUCAUCAUGA Rh [930-948] ORF 186 1455 CCUGCUUCUCAGCGCCUUA 2034 UAAGGCGCUGAGAAGCAGG [241-259] ORF 187 1456 CCCAACCUCUCCCAACUAA 2035 UUAGUUGGGAGAGGUUGG Rh [1895-1913] G 3′UTR 188 1457 UGGGCAGACUCUGGUCAAA 2036 UUUGACCAGAGUCUGCCCA Rh [2007-2025] 3′UTR 189 1458 CUCUGGUCAAGAAGCAUCA 2037 UGAUGCUUCUUGACCAGAG Rh [2015-2033] 3′UTR 190 1459 GAGCCUCUCGAGCGCCUUA 2038 UAAGGCGCUCGAGAGGCUC [1055-1073] ORF 191 1460 AGAAGGCUGUUGCCAUCUA 2039 UAGAUGGCAACAGCCUUCU Rt [1125-1143] ORF 192 1461 CCCUGCUAGUCAACGCCAA 2040 UUGGCGUUGACUAGCAGGG Rh [822-840] ORF 193 1462 GCCUUCAGCUUGUACCAGA 2041 UCUGGUACAAGCUGAAGGC [380-398] ORF 194 1463 GCUGCUAACCAAAGAGCAA 2042 UUGCUCUUUGGUUAGCAGC [1078-1096] ORF 195 1464 CCCACAAGCUCUCCAGCCA 2043 UGGCUGGAGAGCUUGUGGG Rh, D, P [1011-1029] ORF 196 1465 GCUCCCUGCUAUUCAUUGA 2044 UCAAUGAAUAGCAGGGAGC D [1422-1440] ORF 197 1466 GUUCUUCAAAGAUAGGGAA 2045 UUCCCUAUCUUUGAAGAAC [2083-2101] 3′UTR 198 1467 GUCAGCCAGCCCUCUUCUA 2046 UAGAAGAGGGCUGGCUGAC Rh [1841-1859] 3′UTR 199 1468 GCGGGACACCCAAAGCGGA 2047 UCCGCUUUGGGUGUCCCGC [1405-1423] ORF 200 1469 AGCGCAGCGCGCUGCAGUA 2048 UACUGCAGCGCGCUGCGCU Rh, Rt [726-744] ORF 201 1470 CCGGAAACUCCACAUCCUA 2049 UAGGAUGUGGAGUUUCCGG [1701-1719] 3′UTR 202 1471 CCAUUGACAAGAACAAGGA 2050 UCCUUGUUCUUGUCAAUGG Rh, D [1215-1233] ORF 203 1472 GGACAUCUACGGGCGCGAA 2051 UUCGCGCCCGUAGAUGUCC D [1333-1351] ORF 204 1473 GACACAUGGGUGCUAUUGA 2052 UCAAUAGCACCCAUGUGUC Rh, Rt, M [1535-1553] 3′UTR 205 1474 CCUGGCACUGCGGAGAAGA 2053 UCUUCUCCGCAGUGCCAGG [317-335] ORF 206 1475 GGGCCUGACUGAGGCCAUA 2054 UAUGGCCUCAGUCAGGCCC Rt [1201-1219] ORF 207 1476 ACACUGGGAUGAGAAAUUA 2055 UAAUUUCUCAUCCCAGUGU Rh [853-871] ORF 208 1477 GGUCAGCCAGCCCUCUUCA 2056 UGAAGAGGGCUGGCUGACC Rh [1840-1858] 3′UTR 209 1478 GUGAGGCGGAUUGAGAAGA 2057 UCUUCUCAAUCCGCCUCAC [1969-1987] 3′UTR 210 1479 UCACCUGUGAGACCAAAUA 2058 UAUUUGGUCUCACAGGUGA Rh [1811-1829] 3′UTR 211 1480 AGCUGCAAAUCGUGGAGAA 2059 UUCUCCACGAUUUGCAGCU Rh [984-1002] ORF 212 1481 GGUGCACACAGGAUGGCAA 2060 UUGCCAUCCUGUGUGCACC Rh [1495-1513] 3′UTR 213 1482 GGGUGUGGUGGAGGUGACA 2061 UGUCACCUCCACCACACCC Rh, D [1153-1171] ORF 214 1483 CCAGCCUUGGAUACUCCAA 2062 UUGGAGUAUCCAAGGCUGG Rh [1575-1593] 3′UTR 215 1484 CCACAAGCUCUCCAGCCUA 2063 UAGGCUGGAGAGCUUGUGG Rh, D, P [1012-1030] ORF 216 1485 AAAGGCUCCUGAGACACAA 2064 UUGUGUCUCAGGAGCCUUU [1523-1541] 3′UTR 217 1486 AGGAAAAGCUGCAAAUCGA 2065 UCGAUUUGCAGCUUUUCCU Rh [978-996] ORF 218 1487 CGCAGCAGCUCCUGGCACA 2066 UGUGCCAGGAGCUGCUGCG [307-325] ORF 219 1488 GGUGUCAUGAUGAUGCACA 2067 UGUGCAUCAUCAUGACACC Rh [926-944] ORF 220 1489 CCUCUUCUGACACUAAAAA 2068 UUUUUAGUGUCAGAAGAG [1851-1869] G 3′UTR 221 1490 AGCUAGAAUUCACUCCACA 2069 UGUGGAGUGAAUUCUAGCU Rh [1649-1667] 3′UTR 222 1491 CGCUGGGCGGCAAGGCGAA 2070 UUCGCCUUGCCGCCCAGCG [474-492] ORF 223 1492 GGCCUGGCCUUCAGCUUGA 2071 UCAAGCUGAAGGCCAGGCC [374-392] ORF 224 1493 AGACACAUGGGUGCUAUUA 2072 UAAUAGCACCCAUGUGUCU Rh, Rt, M [1534-1552] 3′UTR 225 1494 CGUGGGUGUCAUGAUGAUA 2073 UAUCAUCAUGACACCCACG Rh [922-940] ORF 226 1495 GUGGGUGUCAUGAUGAUGA 2074 UCAUCAUCAUGACACCCAC Rh [923-941] ORF 227 1496 GAGAAGGAGCUCCCAGGAA 2075 UUCCUGGGAGCUCCUUCUC [1981-1999] 3′UTR 228 1497 GACUCUGGUCAAGAAGCAA 2076 UUGCUUCUUGACCAGAGUC Rh [2013-2031] 3′UTR 229 1498 CACUAAAACACCUCAGCUA 2077 UAGCUGAGGUGUUUUAGU [1861-1879] G 3′UTR 230 1499 GGAGGCAUCCAAAGGCUCA 2078 UGAGCCUUUGGAUGCCUCC [1513-1531] 3′UTR 231 1500 GACCCAGCUCAGUGAGCUA 2079 UAGCUCACUGAGCUGGGUC [636-654] ORF 232 1501 CCAUGACCUGCAGAAACAA 2080 UUGUUUCUGCAGGUCAUGG Rh, Rt, M [1171-1189] ORF 233 1502 AGAUGCAGAAGAAGGCUGA 2081 UCAGCCUUCUUCUGCAUCU Rh, Rt, M [1116-1134] ORF 234 1503 CAGCAAGCAGCACUACAAA 2082 UUUGUAGUGCUGCUUGCUG Rh, D [676-694] ORF 235 1504 CAAGCUCUCCAGCCUCAUA 2083 UAUGAGGCUGGAGAGCUUG Rh, D, M, P [1015-1033] ORF 236 1505 UGCAGAAGAAGGCUGUUGA 2084 UCAACAGCCUUCUUCUGCA Rt [1119-1137] ORF 237 1506 GGCGCGAGGAGCUGCGCAA 2085 UUGCGCAGCUCCUCGCGCC Rh, D, M [1344-1362] ORF 238 1507 GGUACCAGCCUUGGAUACA 2086 UGUAUCCAAGGCUGGUACC Rh [1571-1589] 3′UTR 239 1508 GCAGCCGACUGUACGGACA 2087 UGUCCGUACAGUCGGCUGC [621-639] ORF 240 1509 CAGCCUCAUCAUCCUCAUA 2088 UAUGAGGAUGAUGAGGCU Rh, D, Rt, [1024-1042] G M ORF 241 1510 GCCACCGCCUUUGAGUUGA 2089 UCAACUCAAAGGCGGUGGC Rh [1289-1307] ORF 242 1511 AGAAGGACCUGUACCUGGA 2090 UCCAGGUACAGGUCCUUCU Rh, D [1257-1275] ORF 243 1512 GGUGAAGAAACCUGCAGCA 2091 UGCUGCAGGUUUCUUCACC Rh [289-307] ORF 244 1513 GUACCUUCUCACCUGUGAA 2092 UUCACAGGUGAGAAGGUAC Rh [1803-1821] 3′UTR 245 1514 GGCCAAGGACCAGGCAGUA 2093 UACUGCCUGGUCCUUGGCC Rh [403-421] ORF 246 1515 GGCGGCAAGGCGACCACGA 2094 UCGUGGUCGCCUUGCCGCC [479-497] ORF 247 1516 AGCACUCCAAGAUCAACUA 2095 UAGUUGAUCUUGGAGUGCU Rh, D [699-717] ORF 248 1517 AUAUUUAUAGCCAGGUACA 2096 UGUACCUGGCUAUAAAUAU Rh [1789-1807] 3′UTR 249 1518 GGCAGCCGACUGUACGGAA 2097 UUCCGUACAGUCGGCUGCC [620-638] ORF 250 1519 GUCACGCAUGUCAGGCAAA 2098 UUUGCCUGACAUGCGUGAC Rh, D [1240-1258] ORF 251 1520 GACAGGCCUCUACAACUAA 2099 UUAGUUGUAGAGGCCUGUC Rh, Rb, Rt, [946-964] P ORF 252 1521 GAUGCAGAAGAAGGCUGUA 2100 UACAGCCUUCUUCUGCAUC Rh, Rt, M [1117-1135] ORF 253 1522 ACCCAUGACCUGCAGAAAA 2101 UUUUCUGCAGGUCAUGGGU Rh, Rt, M [1169-1187] ORF 254 1523 GGCUUCAUGGUGACUCGGA 2102 UCCGAGUCACCAUGAAGCC Rh [896-914] ORF 255 1524 UGCCUCAAUCAGUAUUCAA 2103 UUGAAUACUGAUUGAGGCA [1772-1790] 3′UTR 256 1525 GUUCUUCAAGCCACACUGA 2104 UCAGUGUGGCUUGAAGAAC Rh, Rb, D [841-859] ORF 257 1526 ACUCCAAGAUCAACUUCCA 2105 UGGAAGUUGAUCUUGGAG Rh, D, Rt, [702-720] U M ORF 258 1527 GCUGUUCUACGCCGACCAA 2106 UUGGUCGGCGUAGAACAGC Rh [1369-1387] ORF 259 1528 UAGUCAACGCCAUGUUCUA 2107 UAGAACAUGGCGUUGACUA Rh [828-846] ORF 260 1529 CCGUGUGCCUGAGCGGACA 2108 UGUCCGCUCAGGCACACGG Rh [1625-1643] 3′UTR 261 1530 AGGCCUCUACAACUACUAA 2109 UUAGUAGUUGUAGAGGCCU Rh, Rb, D, [949-967] Rt, P ORF 262 1531 GCUUCAUGGUGACUCGGUA 2110 UACCGAGUCACCAUGAAGC Rh [897-915] ORF 263 1532 GGUCAAGAAGCAUCGUGUA 2111 UACACGAUGCUUCUUGACC Rh [2019-2037] 3′UTR 264 1533 CUGCGAGCACUCCAAGAUA 2112 UAUCUUGGAGUGCUCGCAG Rh, D [694-712] ORF 265 1534 GUCCUAUACCGUGGGUGUA 2113 UACACCCACGGUAUAGGAC Rh [913-931] ORF 266 1535 GGCCUGACUGAGGCCAUUA 2114 UAAUGGCCUCAGUCAGGCC Rh [1202-1220] ORF 267 1536 CACUCCAAGAUCAACUUCA 2115 UGAAGUUGAUCUUGGAGU Rh, D, Rt, [701-719] G M ORF 268 1537 GCGUCGCAGGCCAAGGCAA 2116 UUGCCUUGGCCUGCGACGC [497-515] ORF 269 1538 AAGGGUGACAAGAUGCGAA 2117 UUCGCAUCUUGUCACCCUU Rh, D [1457-1475] ORF 270 1539 CAAGCUGUUCUACGCCGAA 2118 UUCGGCGUAGAACAGCUUG Rh [1366-1384] ORF 271 1540 CCUGCUAGUCAACGCCAUA 2119 UAUGGCGUUGACUAGCAGG Rh [823-841] ORF 272 1541 CCAAGGGUGUGGUGGAGGA 2120 UCCUCCACCACACCCUUGG Rh, D [1149-1167] ORF 273 1542 CACACAGGAUGGCAGGAGA 2121 UCUCCUGCCAUCCUGUGUG Rh [1499-1517] 3′UTR 274 1543 UCCUGAGACACAUGGGUGA 2122 UCACCCAUGUGUCUCAGGA D, Rt, M [1529-1547] 3′UTR 275 1544 CUACAACUACUACGACGAA 2123 UUCGUCGUAGUAGUUGUAG Rb [955-973] ORF 276 1545 GACAAGAUGCGAGACGAGA 2124 UCUCGUCUCGCAUCUUGUC Rh, Rt [1463-1481] ORF 277 1546 CCUGGAAGCUGGGCAGCCA 2125 UGGCUGCCCAGCUUCCAGG [609-627] ORF 278 1547 CUUCAAGCCACACUGGGAA 2126 UUCCCAGUGUGGCUUGAAG Rh, Rb, D [844-862] ORF 279 1548 GCGAGACGAGUUAUAGGGA 2127 UCCCUAUAACUCGUCUCGC Rh [1471-1489] ORF + 3′UTR 280 1549 GAAGCUGGGCAGCCGACUA 2128 UAGUCGGCUGCCCAGCUUC [613-631] ORF 281 1550 GUGCCUGAGCGGACCUUCA 2129 UGAAGGUCCGCUCAGGCAC Rh [1629-1647] 3′UTR 282 1551 GGUGACCCAUGACCUGCAA 2130 UUGCAGGUCAUGGGUCACC Rh, Rt, M [1165-1183] ORF 283 1552 AUGAGCCUUUGUUGCUAUA 2131 UAUAGCAACAAAGGCUCAU Rh [2114-2132] 3′UTR 284 1553 CAACUACUACGACGACGAA 2132 UUCGUCGUCGUAGUAGUUG Rb [958-976] ORF 285 1554 GCUGCGCUCACUCAGCAAA 2133 UUUGCUGAGUGAGCGCAGC Rh [571-589] ORF 286 1555 GAGAACAUCCUGGUGUCAA 2134 UUGACACCAGGAUGUUCUC [422-440] ORF 287 1556 CCCAAGCUGUUCUACGCCA 2135 UGGCGUAGAACAGCUUGGG Rh [1364-1382] ORF 288 1557 CAGCUCUAUCCCAACCUCA 2136 UGAGGUUGGGAUAGAGCU [1886-1904] G 3′UTR 289 1558 UGAGCUUCGCUGAUGACUA 2137 UAGUCAUCAGCGAAGCUCA Rh [648-666] ORF 290 1559 CCCAAGGCGGCCACGCUUA 2138 UAAGCGUGGCCGCCUUGGG Rh [341-359] ORF 291 1560 CUAUACCGUGGGUGUCAUA 2139 UAUGACACCCACGGUAUAG Rh [916-934] ORF 292 1561 CAUUGACAAGAACAAGGCA 2140 UGCCUUGUUCUUGUCAAUG Rh, D [1216-1234] ORF 293 1562 GGACCCAGCUCAGUGAGCA 2141 UGCUCACUGAGCUGGGUCC [635-653] ORF 294 1563 GACGACGAGAAGGAAAAGA 2142 UCUUUUCCUUCUCGUCGUC Rh [968-986] ORF 295 1564 GCGGCAAGGCGACCACGGA 2143 UCCGUGGUCGCCUUGCCGC [480-498] ORF 296 1565 GGGACACCCAAAGCGGCUA 2144 UAGCCGCUUUGGGUGUCCC [1407-1425] ORF 297 1566 GGGAGGUGAGGUACCAGCA 2145 UGCUGGUACCUCACCUCCC [1562-1580] 3′UTR 298 1567 GCAGCACUACAACUGCGAA 2146 UUCGCAGUUGUAGUGCUGC Rh, D [682-700] ORF 299 1568 GCGCAACGUGACCUGGAAA 2147 UUUCCAGGUCACGUUGCGC M [598-616] ORF 300 1569 GGGCUGGGCCUGACUGAGA 2148 UCUCAGUCAGGCCCAGCCC [1196-1214] ORF 301 1570 CCUGAGCGGACCUUCCCAA 2149 UUGGGAAGGUCCGCUCAGG Rh [1632-1650] 3′UTR 302 1571 GCAGCUGAAGAUCUGGAUA 2150 UAUCCAGAUCUUCAGCUGC Rh, D [1093-1111] ORF 303 1572 AGUGGAGAACAUCCUGGUA 2151 UACCAGGAUGUUCUCCACU Rh [418-436] ORF 304 1573 GCAAGCAGCACUACAACUA 2152 UAGUUGUAGUGCUGCUUGC Rh, D [678-696] ORF 305 1574 AGCUCAGUGAGCUUCGCUA 2153 UAGCGAAGCUCACUGAGCU [641-659] ORF 306 1575 CCGACUUGUCACGCAUGUA 2154 UACAUGCGUGACAAGUCGG Rh [1233-1251] ORF 307 1576 CCGAGGUCACCAAGGACGA 2155 UCGUCCUUGGUGACCUCGG Rh, D [786-804] ORF 308 1577 GGAGCCUCUCGAGCGCCUA 2156 UAGGCGCUCGAGAGGCUCC [1054-1072] ORF 309 1578 GGCCGCGCAGACCACCGAA 2157 UUCGGUGGUCUGCGCGGCC [757-775] ORF 310 1579 GGAAACUCCACAUCCUGUA 2158 UACAGGAUGUGGAGUUUCC Rh [1703-1721] 3′UTR 311 1580 CAAAGCGGCUCCCUGCUAA 2159 UUAGCAGGGAGCCGCUUUG [1415-1433] ORF 312 1581 GCUCCUGAGACACAUGGGA 2160 UCCCAUGUGUCUCAGGAGC D [1527-1545] 3′UTR 313 1582 CCUGGGCCAUAGUCAUUCA 2161 UGAAUGACUAUGGCCCAGG [1725-1743] 3′UTR 314 1583 CGUGGAGCCUCUCGAGCGA 2162 UCGCUCGAGAGGCUCCACG [1051-1069] ORF 315 1584 CCUCCUGCUUCUCAGCGCA 2163 UGCGCUGAGAAGCAGGAGG [238-256] ORF 316 1585 AGUCCCAGAUCAAGCCUGA 2164 UCAGGCUUGAUCUGGGACU Rh [1756-1774] 3′UTR 317 1586 UACCGUGGGUGUCAUGAUA 2165 UAUCAUGACACCCACGGUA Rh [919-937] ORF 318 1587 GCCAGCCCUCUUCUGACAA 2166 UUGUCAGAAGAGGGCUGGC [1845-1863] 3′UTR 319 1588 CCGAGGUGAAGAAACCUGA 2167 UCAGGUUUCUUCACCUCGG Rh, Rt [285-303] ORF 320 1589 UCCUGGCACUGCGGAGAAA 2168 UUUCUCCGCAGUGCCAGGA [316-334] ORF 321 1590 CCCGGAAACUCCACAUCCA 2169 UGGAUGUGGAGUUUCCGGG [1700-1718] 3′UTR 322 1591 ACUCUGGUCAAGAAGCAUA 2170 UAUGCUUCUUGACCAGAGU Rh [2014-2032] 3′UTR 323 1592 CCCAGAUACCAUGAUGCUA 2171 UAGCAUCAUGGUAUCUGGG Rh [1679-1697] 3′UTR 324 1593 CCUGAGACACAUGGGUGCA 2172 UGCACCCAUGUGUCUCAGG D, Rt, M [1530-1548] 3′UTR 325 1594 GCACUACAACUGCGAGCAA 2173 UUGCUCGCAGUUGUAGUGC Rh, D [685-703] ORF 326 1595 CCACAAGAUGGUGGACAAA 2174 UUUGUCCACCAUCUUGUGG Rh, Rb, M, [874-892] P ORF 327 1596 GGACACAGAUGGCAACCCA 2175 UGGGUUGCCAUCUGUGUCC [1306-1324] ORF 328 1597 GAAAAGCUGCUAACCAAAA 2176 UUUUGGUUAGCAGCUUUUC [1073-1091] ORF 329 1598 ACUACAACUGCGAGCACUA 2177 UAGUGCUCGCAGUUGUAGU Rh, D [687-705] ORF 330 1599 GCACUCCAAGAUCAACUUA 2178 UAAGUUGAUCUUGGAGUGC Rh, D [700-718] ORF 331 1600 GCCUUGAAAAGCUGCUAAA 2179 UUUAGCAGCUUUUCAAGGC [1068-1086] ORF 332 1601 GUGACUCGGUCCUAUACCA 2180 UGGUAUAGGACCGAGUCAC Rh [905-923] ORF 333 1602 GUGGUGGAGGUGACCCAUA 2181 UAUGGGUCACCUCCACCAC Rh, Rb, Rt, [1157-1175] M ORF 334 1603 AUGCGAGACGAGUUAUAGA 2182 UCUAUAACUCGUCUCGCAU Rh [1469-1487] ORF + 3′UTR 335 1604 ACCUUCCCAGCUAGAAUUA 2183 UAAUUCUAGCUGGGAAGGU Rh [1641-1659] 3′UTR 336 1605 CCCAGCUAGAAUUCACUCA 2184 UGAGUGAAUUCUAGCUGGG Rh [1646-1664] 3′UTR 337 1606 GGUCACCAAGGACGUGGAA 2185 UUCCACGUCCUUGGUGACC Rh, D [790-808] ORF 338 1607 GGCCUCAGGGUGCACACAA 2186 UUGUGUGCACCCUGAGGCC [1487-1505] 3′UTR 339 1608 UGAGGUACCAGCCUUGGAA 2187 UUCCAAGGCUGGUACCUCA Rh [1568-1586] 3′UTR 340 1609 CAUGGUGACUCGGUCCUAA 2188 UUAGGACCGAGUCACCAUG Rh [901-919] ORF 341 1610 GGUGAGGUACCAGCCUUGA 2189 UCAAGGCUGGUACCUCACC Rh [1566-1584] 3′UTR 342 1611 GCCGAGGUGAAGAAACCUA 2190 UAGGUUUCUUCACCUCGGC Rh, Rt [284-302] ORF 343 1612 GUACGGACCCAGCUCAGUA 2191 UACUGAGCUGGGUCCGUAC [631-649] ORF 344 1613 CAAGAAGGACCUGUACCUA 2192 UAGGUACAGGUCCUUCUUG Rh, D, M [1255-1273] ORF 345 1614 GAGCACUCCAAGAUCAACA 2193 UGUUGAUCUUGGAGUGCUC Rh, D [698-716] ORF 346 1615 CAUGUUCUUCAAGCCACAA 2194 UUGUGGCUUGAAGAACAUG Rh, Rb, D [838-856] ORF 347 1616 CCCUCCUGCUUCUCAGCGA 2195 UCGCUGAGAAGCAGGAGGG [237-255] ORF 348 1617 AUGUCAGGCAAGAAGGACA 2196 UGUCCUUCUUGCCUGACAU Rh, D [1247-1265] ORF 349 1618 CAAGAUCAACUUCCGCGAA 2197 UUCGCGGAAGUUGAUCUUG D [706-724] ORF 350 1619 GCGUGUUCCACGCCACCGA 2198 UCGGUGGCGUGGAACACGC [1278-1296] ORF 351 1620 CGGACCCAGCUCAGUGAGA 2199 UCUCACUGAGCUGGGUCCG [634-652] ORF 352 1621 CCUUCAGCUUGUACCAGGA 2200 UCCUGGUACAAGCUGAAGG [381-399] ORF 353 1622 GCUCUCCAGCCUCAUCAUA 2201 UAUGAUGAGGCUGGAGAGC Rh, D, Rt, [1018-1036] M, P ORF 354 1623 CCCUGGCCCACAAGCUCUA 2202 UAGAGCUUGUGGGCCAGGG Rh, D, P [1005-1023] ORF 355 1624 GCCCGAGGUCACCAAGGAA 2203 UUCCUUGGUGACCUCGGGC Rh, D [784-802] ORF 356 1625 GUGGAGAACAUCCUGGUGA 2204 UCACCAGGAUGUUCUCCAC Rh [419-437] ORF 357 1626 GCUCACUCAGCAACUCCAA 2205 UUGGAGUUGCUGAGUGAGC Rh [576-594] ORF 358 1627 ACGCCAUGUUCUUCAAGCA 2206 UGCUUGAAGAACAUGGCGU Rh, Rb, P [834-852] ORF 359 1628 ACACAUGGGUGCUAUUGGA 2207 UCCAAUAGCACCCAUGUGU Rh [1536-1554] 3′UTR 360 1629 CCAGCUCAGUGAGCUUCGA 2208 UCGAAGCUCACUGAGCUGG [639-657] ORF 361 1630 CCCAGCUCAGUGAGCUUCA 2209 UGAAGCUCACUGAGCUGGG [638-656] ORF 362 1631 GGGCGGCAAGGCGACCACA 2210 UGUGGUCGCCUUGCCGCCC [478-496] ORF 363 1632 CAGGGUGCACACAGGAUGA 2211 UCAUCCUGUGUGCACCCUG [1492-1510] 3′UTR 364 1633 AGGUGAAGAAACCUGCAGA 2212 UCUGCAGGUUUCUUCACCU Rh [288-306] ORF 365 1634 CCUCUCCCAACUAUAAAAA 2213 UUUUUAUAGUUGGGAGAG Rh [1900-1918] G 3′UTR 366 1635 GACUGUACGGACCCAGCUA 2214 UAGCUGGGUCCGUACAGUC [627-645] ORF 367 1636 GAAGGAGCUCCCAGGAGGA 2215 UCCUCCUGGGAGCUCCUUC [1983-2001] 3′UTR 368 1637 ACGCAUGUCAGGCAAGAAA 2216 UUUCUUGCCUGACAUGCGU Rh, D [1243-1261] ORF 369 1638 GACUCGGUCCUAUACCGUA 2217 UACGGUAUAGGACCGAGUC Rh [907-925] ORF 370 1639 CACUACAACUGCGAGCACA 2218 UGUGCUCGCAGUUGUAGUG Rh, D [686-704] ORF 371 1640 AGCUCCUGGCACUGCGGAA 2219 UUCCGCAGUGCCAGGAGCU [313-331] ORF 372 1641 CUAAGGGUGACAAGAUGCA 2220 UGCAUCUUGUCACCCUUAG Rh [1455-1473] ORF 373 1642 UGUGAGACCAAAUUGAGCA 2221 UGCUCAAUUUGGUCUCACA Rh [1816-1834] 3′UTR 374 1643 GCCGACUUGUCACGCAUGA 2222 UCAUGCGUGACAAGUCGGC Rh [1232-1250] ORF 375 1644 CAGGAUGGCAGGAGGCAUA 2223 UAUGCCUCCUGCCAUCCUG [1503-1521] 3′UTR 376 1645 ACAAGAACAAGGCCGACUA 2224 UAGUCGGCCUUGUUCUUGU Rh [1221-1239] ORF 377 1646 UGCGCUCCCUCCUGCUUCA 2225 UGAAGCAGGAGGGAGCGCA [231-249] ORF 378 1647 GGCGAGCUGCUGCGCUCAA 2226 UUGAGCGCAGCAGCUCGCC Rh [563-581] ORF 379 1648 GAUGCACCGGACAGGCCUA 2227 UAGGCCUGUCCGGUGCAUC Rh, Rb, Rt, [937-955] M, P ORF 380 1649 CGUGUCGCUGGGCGGCAAA 2228 UUUGCCGCCCAGCGACACG [469-487] ORF 381 1650 AUCCCAACCUCUCCCAACA 2229 UGUUGGGAGAGGUUGGGA Rh [1893-1911] U 3′UTR 382 1651 UGUUCUACGCCGACCACCA 2230 UGGUGGUCGGCGUAGAACA Rh [1371-1389] ORF 383 1652 CGGCCUGGCCUUCAGCUUA 2231 UAAGCUGAAGGCCAGGCCG [373-391] ORF 384 1653 GUCGCAGGCCAAGGCAGUA 2232 UACUGCCUUGGCCUGCGAC [499-517] ORF 385 1654 AGUCAUUCUGCCUGCCCUA 2233 UAGGGCAGGCAGAAUGACU [1735-1753] 3′UTR 386 1655 CCCAGAAUGACCUGGCCGA 2234 UCGGCCAGGUCAUUCUGGG [1949-1967] 3′UTR 387 1656 ACAAGAUGGUGGACAACCA 2235 UGGUUGUCCACCAUCUUGU Rh, Rb, M, [876-894] P ORF 388 1657 GCUAGUCAACGCCAUGUUA 2236 UAACAUGGCGUUGACUAGC Rh [826-844] ORF 389 1658 ACGCCACCGCCUUUGAGUA 2237 UACUCAAAGGCGGUGGCGU Rh [1287-1305] ORF 390 1659 GCCGCGCAGACCACCGACA 2238 UGUCGGUGGUCUGCGCGGC [758-776] ORF 391 1660 GCUAUUCAUUGGGCGCCUA 2239 UAGGCGCCCAAUGAAUAGC D [1429-1447] ORF 392 1661 CUCAGUGAGCUUCGCUGAA 2240 UUCAGCGAAGCUCACUGAG [643-661] ORF 393 1662 GGAGGUGAGGUACCAGCCA 2241 UGGCUGGUACCUCACCUCC [1563-1581] 3′UTR 394 1663 GCCAAGGCAGUGCUGAGCA 2242 UGCUCAGCACUGCCUUGGC Rh [506-524] ORF 395 1664 CUCUCCAGCCUCAUCAUCA 2243 UGAUGAUGAGGCUGGAGA Rh, D, Rt, [1019-1037] G M, P ORF 396 1665 GAAUGACCUGGCCGCAGUA 2244 UACUGCGGCCAGGUCAUUC [1953-1971] 3′UTR 397 1666 UGGUGACUCGGUCCUAUAA 2245 UUAUAGGACCGAGUCACCA Rh [903-921] ORF 398 1667 CAGGUACCUUCUCACCUGA 2246 UCAGGUGAGAAGGUACCUG Rh [1800-1818] 3′UTR 399 1668 GUUCCACGCCACCGCCUUA 2247 UAAGGCGGUGGCGUGGAAC D [1282-1300] ORF 400 1669 CCGACUGUACGGACCCAGA 2248 UCUGGGUCCGUACAGUCGG [625-643] ORF 401 1670 GCAGACCACCGACGGCAAA 2249 UUUGCCGUCGGUGGUCUGC D, Rt [763-781] ORF 402 1671 AAGAUGCGAGACGAGUUAA 2250 UUAACUCGUCUCGCAUCUU Rh [1466-1484] ORF 403 1672 CAAAGAGCAGCUGAAGAUA 2251 UAUCUUCAGCUGCUCUUUG Rh [1087-1105] ORF 404 1673 ACGACGAGAAGGAAAAGCA 2252 UGCUUUUCCUUCUCGUCGU Rh [969-987] ORF 405 1674 CACUCCACUUGGACAUGGA 2253 UCCAUGUCCAAGUGGAGUG Rh [1659-1677] 3′UTR 406 1675 AGUCCAUCAACGAGUGGGA 2254 UCCCACUCGUUGAUGGACU Rh, Rt, M [741-759] ORF 407 1676 GCGCCGGCCUGGCCUUCAA 2255 UUGAAGGCCAGGCCGGCGC Rh [369-387] ORF 408 1677 GGAAAAGCUGCAAAUCGUA 2256 UACGAUUUGCAGCUUUUCC Rh [979-997] ORF 409 1678 ACAUUUUGUUGGAGCGUGA 2257 UCACGCUCCAACAAAAUGU [2184-2202] 3′UTR 410 1679 ACCGUGGCUUCAUGGUGAA 2258 UUCACCAUGAAGCCACGGU Rh, Rt, M [891-909] ORF 411 1680 CCCUUCAUCUUCCUAGUGA 2259 UCACUAGGAAGAUGAAGGG [1388-1406] ORF 412 1681 GAAAUUCCACCACAAGAUA 2260 UAUCUUGUGGUGGAAUUUC Rh [865-883] ORF 413 1682 CUAUAAAACUAGGUGCUGA 2261 UCAGCACCUAGUUUUAUAG Rh [1910-1928] 3′UTR 414 1683 GGAGGUGCACGCCGGCCUA 2262 UAGGCCGGCGUGCACCUCC [544-562] ORF 415 1684 GCAGGCCAAGGCAGUGCUA 2263 UAGCACUGCCUUGGCCUGC [502-520] ORF 416 1685 UGAGACCAAAUUGAGCUAA 2264 UUAGCUCAAUUUGGUCUCA Rh [1818-1836] 3′UTR 417 1686 GCCAUAGUCAUUCUGCCUA 2265 UAGGCAGAAUGACUAUGGC [1730-1748] 3′UTR 418 1687 AGCUGAAGAUCUGGAUGGA 2266 UCCAUCCAGAUCUUCAGCU Rh, D [1095-1113] ORF 419 1688 CCAUCUCCUUGCCCAAGGA 2267 UCCUUGGGCAAGGAGAUGG Rh [1137-1155] ORF 420 1689 CCCAGAUCAAGCCUGCCUA 2268 UAGGCAGGCUUGAUCUGGG Rh [1759-1777] 3′UTR 421 1690 GCUGUUGCCAUCUCCUUGA 2269 UCAAGGAGAUGGCAACAGC [1130-1148] ORF 422 1691 CGAGGUCACCAAGGACGUA 2270 UACGUCCUUGGUGACCUCG Rh, D [787-805] ORF 423 1692 CAACUAUAAAACUAGGUGA 2271 UCACCUAGUUUUAUAGUUG Rh [1907-1925] 3′UTR 424 1693 GAAGGCUGUUGCCAUCUCA 2272 UGAGAUGGCAACAGCCUUC Rt [1126-1144] ORF 425 1694 UGCGGAGAAGUUGAGCCCA 2273 UGGGCUCAACUUCUCCGCA [325-343] ORF 426 1695 CUCCUUGCCCAAGGGUGUA 2274 UACACCCUUGGGCAAGGAG Rh [1141-1159] ORF 427 1696 GCCCUGAAAGUCCCAGAUA 2275 UAUCUGGGACUUUCAGGGC [1748-1766] 3′UTR 428 1697 CAAGGGUGUGGUGGAGGUA 2276 UACCUCCACCACACCCUUG Rh, D [1150-1168] ORF 429 1698 AAGAGCAGCUGAAGAUCUA 2277 UAGAUCUUCAGCUGCUCUU Rh [1089-1107] ORF 430 1699 GAAGAUGCAGAAGAAGGCA 2278 UGCCUUCUUCUGCAUCUUC Rh, Rb, Rt [1114-1132] ORF 431 1700 CGGAAACUCCACAUCCUGA 2279 UCAGGAUGUGGAGUUUCCG [1702-1720] 3′UTR 432 1701 AGUCAACGCCAUGUUCUUA 2280 UAAGAACAUGGCGUUGACU Rh [829-847] ORF 433 1702 CGAGCGCCUUGAAAAGCUA 2281 UAGCUUUUCAAGGCGCUCG [1063-1081] ORF 434 1703 AUACCGUGGGUGUCAUGAA 2282 UUCAUGACACCCACGGUAU Rh [918-936] ORF 435 1704 GACCUGGGCCAUAGUCAUA 2283 UAUGACUAUGGCCCAGGUC [1723-1741] 3′UTR 436 1705 CAUGUCAGGCAAGAAGGAA 2284 UUCCUUCUUGCCUGACAUG Rh, D [1246-1264] ORF 437 1706 UGCGAGACGAGUUAUAGGA 2285 UCCUAUAACUCGUCUCGCA Rh [1470-1488] ORF + 3′UTR 438 1707 CGCAACGUGACCUGGAAGA 2286 UCUUCCAGGUCACGUUGCG [599-617] ORF 439 1708 AGCAAGCAGCACUACAACA 2287 UGUUGUAGUGCUGCUUGCU Rh, D [677-695] ORF 440 1709 GCUGCUGCGCUCACUCAGA 2288 UCUGAGUGAGCGCAGCAGC Rh [568-586] ORF 441 1710 UGAUGAUGCACCGGACAGA 2289 UCUGUCCGGUGCAUCAUCA Rh [933-951] ORF 442 1711 UUGUUGCUAUCAAUCCAAA 2290 UUUGGAUUGAUAGCAACAA Rh [2122-2140] 3′UTR 443 1712 CCUUGAAAAGCUGCUAACA 2291 UGUUAGCAGCUUUUCAAGG [1069-1087] ORF 444 1713 CCCUUUGACCAGGACAUCA 2292 UGAUGUCCUGGUCAAAGGG Rh, Rt [1322-1340] ORF 445 1714 GAGGUGAAGAAACCUGCAA 2293 UUGCAGGUUUCUUCACCUC Rh [287-305] ORF 446 1715 CCCAAGGGUGUGGUGGAGA 2294 UCUCCACCACACCCUUGGG Rh, D [1148-1166] ORF 447 1716 CCCUGCUAUUCAUUGGGCA 2295 UGCCCAAUGAAUAGCAGGG D [1425-1443] ORF 448 1717 CUGAAAGUCCCAGAUCAAA 2296 UUUGAUCUGGGACUUUCAG [1751-1769] 3′UTR 449 1718 GCUGCAAAUCGUGGAGAUA 2297 UAUCUCCACGAUUUGCAGC Rh [985-1003] ORF 450 1719 CAAGCCUGCCUCAAUCAGA 2298 UCUGAUUGAGGCAGGCUUG Rh [1766-1784] 3′UTR 451 1720 CGAGCAGCUGCGCGACGAA 2299 UUCGUCGCGCAGCUGCUCG [526-544] ORF 452 1721 AGGCCGACUUGUCACGCAA 2300 UUGCGUGACAAGUCGGCCU Rh [1230-1248] ORF 453 1722 GCAGCAGCUCCUGGCACUA 2301 UAGUGCCAGGAGCUGCUGC [308-326] ORF 454 1723 GGCCAUAGUCAUUCUGCCA 2302 UGGCAGAAUGACUAUGGCC [1729-1747] 3′UTR 455 1724 CCCGUGUGCCUGAGCGGAA 2303 UUCCGCUCAGGCACACGGG Rh [1624-1642] 3′UTR 456 1725 CAGCUGAAGAUCUGGAUGA 2304 UCAUCCAGAUCUUCAGCUG Rh, D [1094-1112] ORF 457 1726 CAAGCCACACUGGGAUGAA 2305 UUCAUCCCAGUGUGGCUUG Rh, Rb [847-865] ORF 458 1727 GAAUUCACUCCACUUGGAA 2306 UUCCAAGUGGAGUGAAUUC Rh [1654-1672] 3′UTR 459 1728 CGGCGCCCUGCUAGUCAAA 2307 UUUGACUAGCAGGGCGCCG Rh [817-835] ORF 460 1729 UGGAAGCUGGGCAGCCGAA 2308 UUCGGCUGCCCAGCUUCCA [611-629] ORF 461 1730 GGCAAGGCGACCACGGCGA 2309 UCGCCGUGGUCGCCUUGCC Rh [482-500] ORF 462 1731 CACUGCGGAGAAGUUGAGA 2310 UCUCAACUUCUCCGCAGUG [322-340] ORF 463 1732 GGCAGGAGGCAUCCAAAGA 2311 UCUUUGGAUGCCUCCUGCC [1509-1527] 3′UTR 464 1733 GGUGACUCGGUCCUAUACA 2312 UGUAUAGGACCGAGUCACC Rh [904-922] ORF 465 1734 UUUAUAGCCAGGUACCUUA 2313 UAAGGUACCUGGCUAUAAA Rh [1792-1810] 3′UTR 466 1735 GGCCAUGGCCAAGGACCAA 2314 UUGGUCCUUGGCCAUGGCC Rh, D [397-415] ORF 467 1736 CAAAGAUAGGGAGGGAAGA 2315 UCUUCCCUCCCUAUCUUUG [2089-2107] 3′UTR 468 1737 UCUUCUGACACUAAAACAA 2316 UUGUUUUAGUGUCAGAAG [1853-1871] A 3′UTR 469 1738 CUUCUGACACUAAAACACA 2317 UGUGUUUUAGUGUCAGAA [1854-1872] G 3′UTR 470 1739 UCACGUGGAGCCUCUCGAA 2318 UUCGAGAGGCUCCACGUGA [1048-1066] ORF 471 1740 CAGUCCAUCAACGAGUGGA 2319 UCCACUCGUUGAUGGACUG Rh, Rt, M [740-758] ORF 472 1741 AGACCAAAUUGAGCUAGGA 2320 UCCUAGCUCAAUUUGGUCU [1820-1838] 3′UTR 473 1742 GGGUUCCCGUGUGCCUGAA 2321 UUCAGGCACACGGGAACCC Rh [1619-1637] 3′UTR 474 1743 UUGCUAUCAAUCCAAGAAA 2322 UUUCUUGGAUUGAUAGCAA Rh [2125-2143] 3′UTR 475 1744 CAACCGUGGCUUCAUGGUA 2323 UACCAUGAAGCCACGGUUG Rh, Rt, M [889-907] ORF 476 1745 CUGUACGGACCCAGCUCAA 2324 UUGAGCUGGGUCCGUACAG [629-647] ORF 477 1746 CAGCAGCAAGCAGCACUAA 2325 UUAGUGCUGCUUGCUGCUG Rh, D [673-691] ORF 478 1747 CCUGCAGCCGCAGCAGCUA 2326 UAGCUGCUGCGGCUGCAGG [299-317] ORF 479 1748 GACACUAAAACACCUCAGA 2327 UCUGAGGUGUUUUAGUGUC [1859-1877] 3′UTR 480 1749 CAACUGCGAGCACUCCAAA 2328 UUUGGAGUGCUCGCAGUUG Rh, D [691-709] ORF 481 1750 ACUGCGGAGAAGUUGAGCA 2329 UGCUCAACUUCUCCGCAGU [323-341] ORF 482 1751 GCGCCCUGCUAGUCAACGA 2330 UCGUUGACUAGCAGGGCGC Rh [819-837] ORF 483 1752 GGAAGCUGGGCAGCCGACA 2331 UGUCGGCUGCCCAGCUUCC [612-630] ORF 484 1753 AGGCUCCUGAGACACAUGA 2332 UCAUGUGUCUCAGGAGCCU D [1525-1543] 3′UTR 485 1754 CGACAAGCGCAGCGCGCUA 2333 UAGCGCGCUGCGCUUGUCG [721-739] ORF 486 1755 UCAGUGAGCUUCGCUGAUA 2334 UAUCAGCGAAGCUCACUGA [644-662] ORF 487 1756 UUGAGAAGGAGCUCCCAGA 2335 UCUGGGAGCUCCUUCUCAA [1979-1997] 3′UTR 488 1757 ACUGCGAGCACUCCAAGAA 2336 UUCUUGGAGUGCUCGCAGU Rh, D [693-711] ORF 489 1758 CAUCCUGGUGUCACCCGUA 2337 UACGGGUGACACCAGGAUG [427-445] ORF 490 1759 GUGCGCAGCAGCAAGCAGA 2338 UCUGCUUGCUGCUGCGCAC Rh, D [668-686] ORF 491 1760 CACGCCACCGCCUUUGAGA 2339 UCUCAAAGGCGGUGGCGUG Rh [1286-1304] ORF 492 1761 UCUCGAGCGCCUUGAAAAA 2340 UUUUUCAAGGCGCUCGAGA [1060-1078] ORF 493 1762 GCUUCGCUGAUGACUUCGA 2341 UCGAAGUCAUCAGCGAAGC Rh [651-669] ORF 494 1763 UCUCCUUGCCCAAGGGUGA 2342 UCACCCUUGGGCAAGGAGA Rh [1140-1158] ORF 495 1764 GCAGUCCAUCAACGAGUGA 2343 UCACUCGUUGAUGGACUGC Rh, Rt, M [739-757] ORF 496 1765 AGAUGGUGGACAACCGUGA 2344 UCACGGUUGUCCACCAUCU Rh, M [879-897] ORF 497 1766 CGGCUCCCUGCUAUUCAUA 2345 UAUGAAUAGCAGGGAGCCG [1420-1438] ORF 498 1767 AUACCAUGAUGCUGAGCCA 2346 UGGCUCAGCAUCAUGGUAU [1684-1702] 3′UTR 499 1768 AGCCAGGUACCUUCUCACA 2347 UGUGAGAAGGUACCUGGCU Rh [1797-1815] 3′UTR 500 1769 GAGCCCGGAAACUCCACAA 2348 UUGUGGAGUUUCCGGGCUC [1697-1715] 3′UTR 501 1770 GCAGCUCCUGGCACUGCGA 2349 UCGCAGUGCCAGGAGCUGC [311-329] ORF 502 1771 CCCGAGGUCACCAAGGACA 2350 UGUCCUUGGUGACCUCGGG Rh, D [785-803] ORF 503 1772 CCUGACUGAGGCCAUUGAA 2351 UUCAAUGGCCUCAGUCAGG Rh [1204-1222] ORF 504 1773 UGCUGAGCCCGGAAACUCA 2352 UGAGUUUCCGGGCUCAGCA [1693-1711] 3′UTR 505 1774 GCCAUCUCCUUGCCCAAGA 2353 UCUUGGGCAAGGAGAUGGC Rh [1136-1154] ORF 506 1775 CAAGCAGCACUACAACUGA 2354 UCAGUUGUAGUGCUGCUUG Rh, D [679-697] ORF 507 1776 CAAGGCAGUGCUGAGCGCA 2355 UGCGCUCAGCACUGCCUUG Rh [508-526] ORF 508 1777 CAAUGACAUUUUGUUGGAA 2356 UUCCAACAAAAUGUCAUUG [2179-2197] 3′UTR 509 1778 AGUGAGCUUCGCUGAUGAA 2357 UUCAUCAGCGAAGCUCACU [646-664] ORF 510 1779 AUGAUGAUGCACCGGACAA 2358 UUGUCCGGUGCAUCAUCAU Rh [932-950] ORF 511 1780 GAAACACCUGGCUGGGCUA 2359 UAGCCCAGCCAGGUGUUUC D [1183-1201] ORF 512 1781 CCUGCUAUUCAUUGGGCGA 2360 UCGCCCAAUGAAUAGCAGG D [1426-1444] ORF 513 1782 CGCCACCGCCUUUGAGUUA 2361 UAACUCAAAGGCGGUGGCG Rh [1288-1306] ORF 514 1783 GCUUCUCAGCGCCUUCUGA 2362 UCAGAAGGCGCUGAGAAGC [244-262] ORF 515 1784 UGAUGCUGAGCCCGGAAAA 2363 UUUUCCGGGCUCAGCAUCA [1690-1708] 3′UTR 516 1785 UGACCUGGCCGCAGUGAGA 2364 UCUCACUGCGGCCAGGUCA [1956-1974] 3′UTR 517 1786 UGCAGAAACACCUGGCUGA 2365 UCAGCCAGGUGUUUCUGCA [1179-1197] ORF 518 1787 GCAGUGCUGAGCGCCGAGA 2366 UCUCGGCGCUCAGCACUGC [512-530] ORF 519 1788 CGGCGCGCAACGUGACCUA 2367 UAGGUCACGUUGCGCGCCG [594-612] ORF 520 1789 AGUGCUGAGCGCCGAGCAA 2368 UUGCUCGGCGCUCAGCACU [514-532] ORF 521 1790 ACAGGCCUCUACAACUACA 2369 UGUAGUUGUAGAGGCCUGU Rh, Rb, D, [947-965] Rt, P ORF 522 1791 GCAGCUGCGCGACGAGGAA 2370 UUCCUCGUCGCGCAGCUGC Rh, D [529-547] ORF 523 1792 AUUGAGAAGGAGCUCCCAA 2371 UUGGGAGCUCCUUCUCAAU [1978-1996] 3′UTR 524 1793 CGCGCAGACCACCGACGGA 2372 UCCGUCGGUGGUCUGCGCG [760-778] ORF 525 1794 CCUGUACCUGGCCAGCGUA 2373 UACGCUGGCCAGGUACAGG Rh [1264-1282] ORF 526 1795 CUGAGCGGACCUUCCCAGA 2374 UCUGGGAAGGUCCGCUCAG Rh [1633-1651] 3′UTR 527 1796 GGCCUUCAGCUUGUACCAA 2375 UUGGUACAAGCUGAAGGCC [379-397] ORF 528 1797 CACCCAAAGCGGCUCCCUA 2376 UAGGGAGCCGCUUUGGGUG [1411-1429] ORF 529 1798 GCCAAGGACCAGGCAGUGA 2377 UCACUGCCUGGUCCUUGGC Rh [404-422] ORF 530 1799 CUCAGGGUGCACACAGGAA 2378 UUCCUGUGUGCACCCUGAG [1490-1508] 3′UTR 531 1800 CGAGCUGCUGCGCUCACUA 2379 UAGUGAGCGCAGCAGCUCG Rh [565-583] ORF 532 1801 GGCUGGGCCUGACUGAGGA 2380 UCCUCAGUCAGGCCCAGCC [1197-1215] ORF 533 1802 CCGCAGCAGCUCCUGGCAA 2381 UUGCCAGGAGCUGCUGCGG [306-324] ORF 534 1803 UGUGGGACCUGGGCCAUAA 2382 UUAUGGCCCAGGUCCCACA [1718-1736] 3′UTR 535 1804 AAGAUGCAGAAGAAGGCUA 2383 UAGCCUUCUUCUGCAUCUU Rh, Rt, M [1115-1133] ORF 536 1805 CCACGGCGCGCAACGUGAA 2384 UUCACGUUGCGCGCCGUGG Rh [591-609] ORF 537 1806 ACCUUCUCACCUGUGAGAA 2385 UUCUCACAGGUGAGAAGGU Rh [1805-1823] 3′UTR 538 1807 UGAAGAAACCUGCAGCCGA 2386 UCGGCUGCAGGUUUCUUCA [291-309] ORF 539 1808 CAGCACUACAACUGCGAGA 2387 UCUCGCAGUUGUAGUGCUG Rh, D [683-701] ORF 540 1809 GCGACAAGCGCAGCGCGCA 2388 UGCGCGCUGCGCUUGUCGC [720-738] ORF 541 1810 UAGAAUUCACUCCACUUGA 2389 UCAAGUGGAGUGAAUUCUA Rh [1652-1670] 3′UTR 542 1811 GUGGAAAAACAGACCGGGA 2390 UCCCGGUCUGUUUUUCCAC [1603-1621] 3′UTR 543 1812 ACGUGGAGCCUCUCGAGCA 2391 UGCUCGAGAGGCUCCACGU [1050-1068] ORF 544 1813 GGCGCGCAACGUGACCUGA 2392 UCAGGUCACGUUGCGCGCC [595-613] ORF 545 1814 UGGACAACCGUGGCUUCAA 2393 UUGAAGCCACGGUUGUCCA Rh, M [885-903] ORF 546 1815 CUAGUCAACGCCAUGUUCA 2394 UGAACAUGGCGUUGACUAG Rh [827-845] ORF 547 1816 AGAAUGACCUGGCCGCAGA 2395 UCUGCGGCCAGGUCAUUCU [1952-1970] 3′UTR 548 1817 AGCUGCUGCGCUCACUCAA 2396 UUGAGUGAGCGCAGCAGCU Rh [567-585] ORF 549 1818 CUCUAUCCCAACCUCUCCA 2397 UGGAGAGGUUGGGAUAGA Rh [1889-1907] G 3′UTR 550 1819 GCGAGCUGCUGCGCUCACA 2398 UGUGAGCGCAGCAGCUCGC Rh [564-582] ORF 551 1820 CGCAGCAGCAAGCAGCACA 2399 UGUGCUGCUUGCUGCUGCG Rh, D [671-689] ORF 552 1821 GGCUGGGCUGGGCCUGACA 2400 UGUCAGGCCCAGCCCAGCC [1192-1210] ORF 553 1822 UCUCCAGCCUCAUCAUCCA 2401 UGGAUGAUGAGGCUGGAG Rh, D, Rt, [1020-1038] A M ORF 554 1823 CAACGCCAUGUUCUUCAAA 2402 UUUGAAGAACAUGGCGUUG Rh, Rb, P [832-850] ORF 555 1824 UGGCACUGCGGAGAAGUUA 2403 UAACUUCUCCGCAGUGCCA [319-337] ORF 556 1825 UUUGAGUUGGACACAGAUA 2404 UAUCUGUGUCCAACUCAAA [1298-1316] ORF 557 1826 UGGGCGAGCUGCUGCGCUA 2405 UAGCGCAGCAGCUCGCCCA Rh [561-579] ORF 558 1827 CUGCUAACCAAAGAGCAGA 2406 UCUGCUCUUUGGUUAGCAG [1079-1097] ORF 559 1828 AACGUGACCUGGAAGCUGA 2407 UCAGCUUCCAGGUCACGUU [602-620] ORF 560 1829 AUGACAUUUUGUUGGAGCA 2408 UGCUCCAACAAAAUGUCAU [2181-2199] 3′UTR 561 1830 CAGGAGGCAUCCAAAGGCA 2409 UGCCUUUGGAUGCCUCCUG [1511-1529] 3′UTR 562 1831 AUCUCCUUGCCCAAGGGUA 2410 UACCCUUGGGCAAGGAGAU Rh [1139-1157] ORF 563 1832 UGGGAUGAGAAAUUCCACA 2411 UGUGGAAUUUCUCAUCCCA Rh [857-875] ORF 564 1833 AAAGCUGCUAACCAAAGAA 2412 UUCUUUGGUUAGCAGCUUU [1075-1093] ORF 565 1834 AGGAGGCAUCCAAAGGCUA 2413 UAGCCUUUGGAUGCCUCCU [1512-1530] 3′UTR 566 1835 CACCGCCUUUGAGUUGGAA 2414 UUCCAACUCAAAGGCGGUG Rh [1291-1309] ORF 567 1836 CCAACUAUAAAACUAGGUA 2415 UACCUAGUUUUAUAGUUGG Rh [1906-1924] 3′UTR 568 1837 CAAGAAGCAUCGUGUCUGA 2416 UCAGACACGAUGCUUCUUG Rh [2022-2040] 3′UTR 569 1838 AGCAGCUGAAGAUCUGGAA 2417 UUCCAGAUCUUCAGCUGCU Rh, D [1092-1110] ORF 570 1839 GCGCUCCCUCCUGCUUCUA 2418 UAGAAGCAGGAGGGAGCGC [232-250] ORF 571 1840 UGCUAGUCAACGCCAUGUA 2419 UACAUGGCGUUGACUAGCA Rh [825-843] ORF 572 1841 CGCCGAGCAGCUGCGCGAA 2420 UUCGCGCAGCUGCUCGGCG [523-541] ORF 573 1842 CCGCGCAGACCACCGACGA 2421 UCGUCGGUGGUCUGCGCGG [759-777] ORF 574 1843 UAGCCAGGUACCUUCUCAA 2422 UUGAGAAGGUACCUGGCUA Rh [1796-1814] 3′UTR 575 1844 UGCUUCUCAGCGCCUUCUA 2423 UAGAAGGCGCUGAGAAGCA [243-261] ORF 576 1845 CUCCCUCCUGCUUCUCAGA 2424 UCUGAGAAGCAGGAGGGAG [235-253] ORF 577 1846 CGCAGGCCAAGGCAGUGCA 2425 UGCACUGCCUUGGCCUGCG [501-519] ORF 578 1847 GCAAGGCGACCACGGCGUA 2426 UACGCCGUGGUCGCCUUGC Rh [483-501] ORF 579 1848 GCAGCCGCAGCAGCUCCUA 2427 UAGGAGCUGCUGCGGCUGC [302-320] ORF

TABLE E SERPINH1 Cross-Species 18 + 1-mer siRNAs human- 32454740 SEQ Other ORF: 230- No. SEQ ID NO Sense siRNA ID NO AntiSense siRNA Species 1486 1 2428 UCACCAAGGACGUGGAGCA 2576 UGCUCCACGUCCUUGGU Rh, D [792-810] GA ORF 2 2429 CAGCGCGCUGCAGUCCAUA 2577 UAUGGACUGCAGCGCGC Rh, Rt [730-748] UG ORF 3 2430 CAUCUACGGGCGCGAGGAA 2578 UUCCUCGCGCCCGUAGA D, M [1336-1354] UG ORF 4 2431 CUCCAGCCUCAUCAUCCUA 2579 UAGGAUGAUGAGGCUGG Rh, D, Rt, M [1021-1039] AG ORF 5 2432 GACAUCUACGGGCGCGAGA 2580 UCUCGCGCCCGUAGAUG D, M [1334-1352] UC ORF 6 2433 CGUGCGCAGCAGCAAGCAA 2581 UUGCUUGCUGCUGCGCA Rh, D, M [667-685] CG ORF 7 2434 GUCACCAAGGACGUGGAGA 2582 UCUCCACGUCCUUGGUG Rh, D [791-809] AC ORF 8 2435 CCGCGACAAGCGCAGCGCA 2583 UGCGCUGCGCUUGUCGC D [718-736] GG ORF 9 2436 GCGCAGCGCGCUGCAGUCA 2584 UGACUGCAGCGCGCUGC Rh, Rt [727-745] GC ORF 10 2437 GGCCCACAAGCUCUCCAGA 2585 UCUGGAGAGCUUGUGGG Rh, D, P [1009-1027] CC ORF 11 2438 CAAGGACGUGGAGCGCACA 2586 UGUGCGCUCCACGUCCU Rh, D [796-814] UG ORF 12 2439 AGCCUCAUCAUCCUCAUGA 2587 UCAUGAGGAUGAUGAGG Rh, D, Rt, M [1025-1043] CU ORF 13 2440 GGUGUGGUGGAGGUGACCA 2588 UGGUCACCUCCACCACA Rh, D [1154-1172] CC ORF 14 2441 GCAAGCUGCCCGAGGUCAA 2589 UUGACCUCGGGCAGCUU Rh, D [777-795] GC ORF 15 2442 GUGGAGGUGACCCAUGACA 2590 UGUCAUGGGUCACCUCC Rh, Rt, M [1160-1178] AC ORF 16 2443 CACAAGAUGGUGGACAACA 2591 UGUUGUCCACCAUCUUG Rh, Rb, M, P [875-893] UG ORF 17 2444 GCGAGGAGCUGCGCAGCCA 2592 UGGCUGCGCAGCUCCUC D, M [1347-1365] GC ORF 18 2445 UACUACGACGACGAGAAGA 2593 UCUUCUCGUCGUCGUAG Rb [962-980] UA ORF 19 2446 GAGGUGACCCAUGACCUGA 2594 UCAGGUCAUGGGUCACC Rh, Rt, M [1163-1181] UC ORF 20 2447 ACUUCCGCGACAAGCGCAA 2595 UUGCGCUUGUCGCGGAA D [714-732] GU ORF 21 2448 GCCCACAAGCUCUCCAGCA 2596 UGCUGGAGAGCUUGUGG Rh, D, P [1010-1028] GC ORF 22 2449 GCGCAGCAGCAAGCAGCAA 2597 UUGCUGCUUGCUGCUGC Rh, D [670-688] GC ORF 23 2450 CGAGGAGCUGCGCAGCCCA 2598 UGGGCUGCGCAGCUCCU D, M [1348-1366] CG ORF 24 2451 AACGCCAUGUUCUUCAAGA 2599 UCUUGAAGAACAUGGCG Rh, Rb, P [833-851] UU ORF 25 2452 GUCAGGCAAGAAGGACCUA 2600 UAGGUCCUUCUUGCCUG Rh, D [1249-1267] AC ORF 26 2453 GCCUGGGCGAGCUGCUGCA 2601 UGCAGCAGCUCGCCCAG Rh, D [558-576] GC ORF 27 2454 GAUGAUGCACCGGACAGGA 2602 UCCUGUCCGGUGCAUCA Rh, Rb, Rt, M [934-952] UC ORF 28 2455 GGACCUGUACCUGGCCAGA 2603 UCUGGCCAGGUACAGGU Rh, D [1261-1279] CC ORF 29 2456 GCGACGAGGAGGUGCACGA 2604 UCGUGCACCUCCUCGUC D [537-555] GC ORF 30 2457 UGUGGUGGAGGUGACCCAA 2605 UUGGGUCACCUCCACCA Rh, D [1156-1174] CA ORF 31 2458 UUCAAGCCACACUGGGAUA 2606 UAUCCCAGUGUGGCUUG Rh, Rb [845-863] AA ORF 32 2459 CAAGAUGGUGGACAACCGA 2607 UCGGUUGUCCACCAUCU Rh, Rb, M, P [877-895] UG ORF 33 2460 UCAACUUCCGCGACAAGCA 2608 UGCUUGUCGCGGAAGUU D [711-729] GA ORF 34 2461 AUUCAUUGGGCGCCUGGUA 2609 UACCAGGCGCCCAAUGA D [1432-1450] AU ORF 35 2462 CUCCAAGAUCAACUUCCGA 2610 UCGGAAGUUGAUCUUGG Rh, D, Rt, M [703-721] AG ORF 36 2463 CAGGCCAUGGCCAAGGACA 2611 UGUCCUUGGCCAUGGCC Rh, D [395-413] UG ORF 37 2464 GUACCAGGCCAUGGCCAAA 2612 UUUGGCCAUGGCCUGGU Rh, D [391-409] AC ORF 38 2465 UGUCAGGCAAGAAGGACCA 2613 UGGUCCUUCUUGCCUGA Rh, D [1248-1266] CA ORF 39 2466 CUUCGUGCGCAGCAGCAAA 2614 UUUGCUGCUGCGCACGA Rh, D, M [664-682] AG ORF 40 2467 CAACUUCCGCGACAAGCGA 2615 UCGCUUGUCGCGGAAGU D [712-730] UG ORF 41 2468 CCACCACAAGAUGGUGGAA 2616 UUCCACCAUCUUGUGGU Rh, Rb, D, P [871-889] GG ORF 42 2469 GCGCGACGAGGAGGUGCAA 2617 UUGCACCUCCUCGUCGC Rh, D [535-553] GC ORF 43 2470 CUACAACUGCGAGCACUCA 2618 UGAGUGCUCGCAGUUGU Rh, D [688-706] AG ORF 44 2471 UGGAGGUGACCCAUGACCA 2619 UGGUCAUGGGUCACCUC Rh, Rt, M [1161-1179] CA ORF 45 2472 GAGGUCACCAAGGACGUGA 2620 UCACGUCCUUGGUGACC Rh, D [788-806] UC ORF 46 2473 AAGAAGGACCUGUACCUGA 2621 UCAGGUACAGGUCCUUC Rh, D [1256-1274] UU ORF 47 2474 GACAACCGUGGCUUCAUGA 2622 UCAUGAAGCCACGGUUG Rh, Rt, M [887-905] UC ORF 48 2475 ACCAGGACAUCUACGGGCA 2623 UGCCCGUAGAUGUCCUG D, Rt [1329-1347] GU ORF 49 2476 GCUGCCCGAGGUCACCAAA 2624 UUUGGUGACCUCGGGCA Rh, D [781-799] GC ORF 50 2477 AUGCAGAAGAAGGCUGUUA 2625 UAACAGCCUUCUUCUGC Rt [1118-1136] AU ORF 51 2478 GGCCUGGGCGAGCUGCUGA 2626 UCAGCAGCUCGCCCAGG Rh, D [557-575] CC ORF 52 2479 GAUGGUGGACAACCGUGGA 2627 UCCACGGUUGUCCACCA Rh, M [880-898] UC ORF 53 2480 CUCCCUGCUAUUCAUUGGA 2628 UCCAAUGAAUAGCAGGG D [1423-1441] AG ORF 54 2481 GAAGGACCUGUACCUGGCA 2629 UGCCAGGUACAGGUCCU Rh, D [1258-1276] UC ORF 55 2482 CCACCGACGGCAAGCUGCA 2630 UGCAGCUUGCCGUCGGU D, Rt [768-786] GG ORF 56 2483 UGCUAUUCAUUGGGCGCCA 2631 UGGCGCCCAAUGAAUAG D [1428-1446] CA ORF 57 2484 AUGUUCUUCAAGCCACACA 2632 UGUGUGGCUUGAAGAAC Rh, Rb, D [839-857] AU ORF 58 2485 CCAGGACAUCUACGGGCGA 2633 UCGCCCGUAGAUGUCCU D, Rt [1330-1348] GG ORF 59 2486 GCGCGAGGAGCUGCGCAGA 2634 UCUGCGCAGCUCCUCGC Rh, D, M [1345-1363] GC ORF 60 2487 GAGCAGCUGCGCGACGAGA 2635 UCUCGUCGCGCAGCUGC Rh, D [527-545] UC ORF 61 2488 CUAUUCAUUGGGCGCCUGA 2636 UCAGGCGCCCAAUGAAU D [1430-1448] AG ORF 62 2489 ACAAGCUCUCCAGCCUCAA 2637 UUGAGGCUGGAGAGCUU Rh, D, M, P [1014-1032] GU ORF 63 2490 GCUGAAGAUCUGGAUGGGA 2638 UCCCAUCCAGAUCUUCA Rh, D [1096-1114] GC ORF 64 2491 GACCAGGACAUCUACGGGA 2639 UCCCGUAGAUGUCCUGG D, Rt [1328-1346] UC ORF 65 2492 CAAGCGCAGCGCGCUGCAA 2640 UUGCAGCGCGCUGCGCU Rh, Rt [724-742] UG ORF 66 2493 CCAUGGCCAAGGACCAGGA 2641 UCCUGGUCCUUGGCCAU Rh, D [399-417] GG ORF 67 2494 CACCAAGGACGUGGAGCGA 2642 UCGCUCCACGUCCUUGG Rh, D [793-811] UG ORF 68 2495 CCGUGGCUUCAUGGUGACA 2643 UGUCACCAUGAAGCCAC Rh, Rt, M [892-910] GG ORF 69 2496 UGACCAGGACAUCUACGGA 2644 UCCGUAGAUGUCCUGGU Rt [1327-1345] CA ORF 70 2497 AGACCACCGACGGCAAGCA 2645 UGCUUGCCGUCGGUGGU D, Rt [765-783] CU ORF 71 2498 GACAAGCGCAGCGCGCUGA 2646 UCAGCGCGCUGCGCUUG Rh, Rt [722-740] UC ORF 72 2499 AGAAACACCUGGCUGGGCA 2647 UGCCCAGCCAGGUGUUU D [1182-1200] CU ORF 73 2500 AAGAUGGUGGACAACCGUA 2648 UACGGUUGUCCACCAUC Rh, M [878-896] UU ORF 74 2501 CAGACCACCGACGGCAAGA 2649 UCUUGCCGUCGGUGGUC D, Rt [764-782] UG ORF 75 2502 AGGACCUGUACCUGGCCAA 2650 UUGGCCAGGUACAGGUC Rh, D [1260-1278] CU ORF 76 2503 CUGCUAUUCAUUGGGCGCA 2651 UGCGCCCAAUGAAUAGC D [1427-1445] AG ORF 77 2504 GUCCAUCAACGAGUGGGCA 2652 UGCCCACUCGUUGAUGG Rh, Rt, M [742-760] AC ORF 78 2505 CCAGGCCAUGGCCAAGGAA 2653 UUCCUUGGCCAUGGCCU Rh, D [394-412] GG ORF 79 2506 AAGCAGCACUACAACUGCA 2654 UGCAGUUGUAGUGCUGC Rh, D [680-698] UU ORF 80 2507 UGUUCCACGCCACCGCCUA 2655 UAGGCGGUGGCGUGGAA D [1281-1299] CA ORF 81 2508 UACAACUACUACGACGACA 2656 UGUCGUCGUAGUAGUUG Rb [956-974] UA ORF 82 2509 CCUCAUCAUCCUCAUGCCA 2657 UGGCAUGAGGAUGAUGA Rh, D, Rt, M [1027-1045] GG ORF 83 2510 UGGUGGACAACCGUGGCUA 2658 UAGCCACGGUUGUCCAC Rh, M [882-900] CA ORF 84 2511 GACCACCGACGGCAAGCUA 2659 UAGCUUGCCGUCGGUGG D, Rt [766-784] UC ORF 85 2512 AGCUGCGCGACGAGGAGGA 2660 UCCUCCUCGUCGCGCAG Rh, D [531-549] CU ORF 86 2513 CGGCAAGCUGCCCGAGGUA 2661 UACCUCGGGCAGCUUGC Rh, D [775-793] CG ORF 87 2514 UGGCCCACAAGCUCUCCAA 2662 UUGGAGAGCUUGUGGGC Rh, D, P [1008-1026] CA ORF 88 2515 CAGCUGCGCGACGAGGAGA 2663 UCUCCUCGUCGCGCAGC Rh, D [530-548] UG ORF 89 2516 CUUCCGCGACAAGCGCAGA 2664 UCUGCGCUUGUCGCGGA D [715-733] AG ORF 90 2517 UGGGCCUGACUGAGGCCAA 2665 UUGGCCUCAGUCAGGCC Rt [1200-1218] CA ORF 91 2518 GCUGCGCGACGAGGAGGUA 2666 UACCUCCUCGUCGCGCA Rh, D [532-550] GC ORF 92 2519 CAGGACAUCUACGGGCGCA 2667 UGCGCCCGUAGAUGUCC D [1331-1349] UG ORF 93 2520 GCCAUGGCCAAGGACCAGA 2668 UCUGGUCCUUGGCCAUG Rh, D [398-416] GC ORF 94 2521 UCCAAGAUCAACUUCCGCA 2669 UGCGGAAGUUGAUCUUG D [704-722] GA ORF 95 2522 ACCACCGACGGCAAGCUGA 2670 UCAGCUUGCCGUCGGUG D, Rt [767-785] GU ORF 96 2523 AUCUACGGGCGCGAGGAGA 2671 UCUCCUCGCGCCCGUAG D, M [1337-1355] AU ORF 97 2524 CUGCCCGAGGUCACCAAGA 2672 UCUUGGUGACCUCGGGC Rh, D [782-800] AG ORF 98 2525 AUCAACUUCCGCGACAAGA 2673 UCUUGUCGCGGAAGUUG D [710-728] AU ORF 99 2526 UCAUUGGGCGCCUGGUCCA 2674 UGGACCAGGCGCCCAAU Rh, D [1434-1452] GA ORF 100 2527 CAUUGGGCGCCUGGUCCGA 2675 UCGGACCAGGCGCCCAA Rh, D [1435-1453] UG ORF 101 2528 GUGUUCCACGCCACCGCCA 2676 UGGCGGUGGCGUGGAAC D [1280-1298] AC ORF 102 2529 AUGAUGCACCGGACAGGCA 2677 UGCCUGUCCGGUGCAUC Rh, Rb, Rt, [935-953] AU M, P ORF 103 2530 CGACGAGGAGGUGCACGCA 2678 UGCGUGCACCUCCUCGU D [538-556] CG ORF 104 2531 CAGAAACACCUGGCUGGGA 2679 UCCCAGCCAGGUGUUUC D [1181-1199] UG ORF 105 2532 UGAUGCACCGGACAGGCCA 2680 UGGCCUGUCCGGUGCAU Rh, Rb, Rt, [936-954] CA M, P ORF 106 2533 AAGGCUGUUGCCAUCUCCA 2681 UGGAGAUGGCAACAGCC D, Rt [1127-1145] UU ORF 107 2534 AUGACUUCGUGCGCAGCAA 2682 UUGCUGCGCACGAAGUC Rh, Rt, M [660-678] AU ORF 108 2535 UCAGGCAAGAAGGACCUGA 2683 UCAGGUCCUUCUUGCCU Rh, D [1250-1268] GA ORF 109 2536 CUCAUCAUCCUCAUGCCCA 2684 UGGGCAUGAGGAUGAUG Rh, Rt, M [1028-1046] AG ORF 110 2537 CGCGACGAGGAGGUGCACA 2685 UGUGCACCUCCUCGUCG Rh, D [536-554] CG ORF 111 2538 ACAACCGUGGCUUCAUGGA 2686 UCCAUGAAGCCACGGUU Rh, Rt, M [888-906] GU ORF 112 2539 UUGACCAGGACAUCUACGA 2687 UCGUAGAUGUCCUGGUC Rt [1326-1344] AA ORF 113 2540 CAAGCUGCCCGAGGUCACA 2688 UGUGACCUCGGGCAGCU Rh, D [778-796] UG ORF 114 2541 UCCCUGCUAUUCAUUGGGA 2689 UCCCAAUGAAUAGCAGG D [1424-1442] GA ORF 115 2542 UAUUCAUUGGGCGCCUGGA 2690 UCCAGGCGCCCAAUGAA D [1431-1449] UA ORF 116 2543 CUGCGCGACGAGGAGGUGA 2691 UCACCUCCUCGUCGCGC Rh, D [533-551] AG ORF 117 2544 CUACGGGCGCGAGGAGCUA 2692 UAGCUCCUCGCGCCCGU D, M [1339-1357] AG ORF 118 2545 CGCGAGGAGCUGCGCAGCA 2693 UGCUGCGCAGCUCCUCG D, M [1346-1364] CG ORF 119 2546 ACACCUGGCUGGGCUGGGA 2694 UCCCAGCCCAGCCAGGU D [1186-1204] GU ORF 120 2547 UCUACGGGCGCGAGGAGCA 2695 UGCUCCUCGCGCCCGUA D, M [1338-1356] GA ORF 121 2548 UUCUUCAAGCCACACUGGA 2696 UCCAGUGUGGCUUGAAG Rh, Rb, D [842-860] AA ORF 122 2549 CCUGGGCGAGCUGCUGCGA 2697 UCGCAGCAGCUCGCCCA Rh, D [559-577] GG ORF 123 2550 AAGAAGGCUGUUGCCAUCA 2698 UGAUGGCAACAGCCUUC Rt [1124-1142] UU ORF 124 2551 CGACGGCAAGCUGCCCGAA 2699 UUCGGGCAGCUUGCCGU D [772-790] CG ORF 125 2552 GACGGCAAGCUGCCCGAGA 2700 UCUCGGGCAGCUUGCCG Rh, D [773-791] UC ORF 126 2553 UUCAUUGGGCGCCUGGUCA 2701 UGACCAGGCGCCCAAUG Rh, D [1433-1451] AA ORF 127 2554 AAGCGCAGCGCGCUGCAGA 2702 UCUGCAGCGCGCUGCGC Rh, Rt [725-743] UU ORF 128 2555 CCUGGCCCACAAGCUCUCA 2703 UGAGAGCUUGUGGGCCA Rh, D, P [1006-1024] GG ORF 129 2556 ACGGCAAGCUGCCCGAGGA 2704 UCCUCGGGCAGCUUGCC Rh, D [774-792] GU ORF 130 2557 UUUGACCAGGACAUCUACA 2705 UGUAGAUGUCCUGGUCA Rt [1325-1343] AA ORF 131 2558 UGACUUCGUGCGCAGCAGA 2706 UCUGCUGCGCACGAAGU Rh, Rt, M [661-679] CA ORF 132 2559 AAGGACGUGGAGCGCACGA 2707 UCGUGCGCUCCACGUCC Rh, D [797-815] UU ORF 133 2560 UCCAUCAACGAGUGGGCCA 2708 UGGCCCACUCGUUGAUG Rt, M [743-761] GA ORF 134 2561 CACCGACGGCAAGCUGCCA 2709 UGGCAGCUUGCCGUCGG D, Rt [769-787] UG ORF 135 2562 ACGGGCGCGAGGAGCUGCA 2710 UGCAGCUCCUCGCGCCC D, M [1341-1359] GU ORF 136 2563 UCCGCGACAAGCGCAGCGA 2711 UCGCUGCGCUUGUCGCG D [717-735] GA ORF 137 2564 UUGGGCGCCUGGUCCGGCA 2712 UGCCGGACCAGGCGCCC Rh, D [1437-1455] AA ORF 138 2565 AUGGUGGACAACCGUGGCA 2713 UGCCACGGUUGUCCACC Rh, M [881-899] AU ORF 139 2566 AUUGGGCGCCUGGUCCGGA 2714 UCCGGACCAGGCGCCCA Rh, D [1436-1454] AU ORF 140 2567 UACGGGCGCGAGGAGCUGA 2715 UCAGCUCCUCGCGCCCG D, M [1340-1358] UA ORF 141 2568 AUGCACCGGACAGGCCUCA 2716 UGAGGCCUGUCCGGUGC Rh, Rb, Rt, P [938-956] AU ORF 142 2569 UUCCACCACAAGAUGGUGA 2717 UCACCAUCUUGUGGUGG Rh, Rb, D, P [869-887] AA ORF 143 2570 UUCCGCGACAAGCGCAGCA 2718 UGCUGCGCUUGUCGCGG D [716-734] AA ORF 144 2571 UACCAGGCCAUGGCCAAGA 2719 UCUUGGCCAUGGCCUGG Rh, D [392-410] UA ORF 145 2572 AAACACCUGGCUGGGCUGA 2720 UCAGCCCAGCCAGGUGU D [1184-1202] UU ORF 146 2573 ACCGACGGCAAGCUGCCCA 2721 UGGGCAGCUUGCCGUCG D [770-788] GU ORF 147 2574 AACACCUGGCUGGGCUGGA 2722 UCCAGCCCAGCCAGGUG D [1185-1203] UU ORF 148 2575 UUCGUGCGCAGCAGCAAGA 2723 UCUUGCUGCUGCGCACG Rh, D, M [665-683] AA ORF

The most active sequences were selected from further assays. From Table 4 siRNA compounds SERPINH1_(—)2, SERPINH1_(—)6, SERPINH1_(—)13, SERPINH1_(—)45 SERPINH1_(—)45a, SERPINH1_(—)51, SERPINH1_(—)51a, SERPINH1_(—)52 and SERPINH1_(—)86 were selected as preferred compounds (Tables 6-A and B).

TABLE 6-A Select siRNAs SEQ ID Activity Activity Activity siRNA SEQ ID SEN AS 0.1 nM 0.5 nM 5 nM IC50 (nM) Length SERPINH1_2 60 127 65 48 7 .008 19 SERPINH1_6 63 130 164 39 5 .019 19 SERPINH1_11 68 135 119 54 6 .05 19 SERPINH1_13 69 136 91 24 4 19 SERPINH1_45 97 164 156 38 8 .07 19 SERPINH1_45a 98 165 19 SERPINH1_51 101 168 68 39 5 .05 19 SERPINH1_52 102 169 149 37 9 0.06 19 SERPINH1_86 123 190 121 61 0.27 19

TABLE 6B SEQ SEQ Activity Activity Activity Activity (2) Activity ID ID 0.026 0.077 0.23 0.69 ctivity 6.25 Act siRNA SEN AS nM nM nM nM 2.1 nM nM 25 SERPINH1_45 97 164 102 81   55 41 28 22 16 SERPINH1_45a 98 165 107 8 (3) 84 69 36 24 16

From Table 5 siRNA compounds SERPINH1_(—)4, SERPINH1_(—)12, SERPINH1_(—)18, SERPINH1_(—)30, SERPINH1_(—)58 and SERPINH1_(—)88 were selected as preferred compounds (Table 7).

TABLE 7 Select siRNAs SEQ SEQ Activity Activity Activity siRNA ID NO SEN ID NO AS 0.1 nM 0.5 nM 5 nM IC50 (nM) Length SERPINH1_4 195 220 60 35 5 .006 19 SERPINH1_12 196 221 54 42 8 .065 19 SERPINH1_18 197 222 139 43 9 19 SERPINH1_30 199 224 146 49 9 0.093 19 SERPINH1_58 208 233 na na 8 19 SERPINH1_88 217 242 105 43 9 19

Example 21 Animal model systems of fibrotic conditions

Testing the active siRNAs of the description may be done in predictive animal models. Rat diabetic and aging models of kidney fibrosis include Zucker diabetic fatty (ZDF) rats, aged fa/fa (obese Zucker) rats, aged Sprague-Dawley (SD) rats, and Goto Kakizaki (GK) rats; GK rats are an inbred strain derived from Wistar rats, selected for spontaneous development of NIDDM (diabetes type II). Induced models of kidney fibrosis include the permanent unilateral ureteral obstruction (UUO) model which is a model of acute interstitial fibrosis occurring in healthy non-diabetic animals; renal fibrosis develops within days following the obstruction. Another induced model of kidney fibrosis is 5/6 nephrectomy.

Two models of liver fibrosis in rats are the Bile Duct Ligation (BDL) with sham operation as controls, and CC14 poisoning, with olive oil fed animals as controls, as described in the following references: Lotersztajn S, et al Hepatic Fibrosis: Molecular Mechanisms and Drug Targets. Annu Rev Pharmacol Toxicol. 2004 Oct. 7; Uchio K, et al., Down-regulation of connective tissue growth factor and type I collagen mRNA expression by connective tissue growth factor antisense oligonucleotide during experimental liver fibrosis. Wound Repair Regen. 2004 January-February; 12(1):60-6; Xu X Q, et al., Molecular classification of liver cirrhosis in a rat model by proteomics and bioinformatics Proteomics. 2004 October; 4(10):3235-45.

Models for ocular scarring are well known in the art e.g. Sherwood M B et al., J Glaucoma. 2004 October; 13(5):407-12. A new model of glaucoma filtering surgery in the rat; Miller M H et al., Ophthalmic Surg. 1989 May; 20(5):350-7. Wound healing in an animal model of glaucoma fistulizing surgery in the Rb; vanBockxmeer F M et al., Retina. 1985 Fall-Winter; 5(4): 239-52. Models for assessing scar tissue inhibitors; Wiedemann P et al., J Pharmacol Methods. 1984 August; 12(1): 69-78. Proliferative vitreoretinopathy: the Rb cell injection model for screening of antiproliferative drugs.

Models of cataract are described in the following publications: Zhou et al., 2002. Invest Ophthalmol Vis Sci. 43:2293-300; Wang et al. 2004 Curr Eye Res. 29:51-58.

The compounds of Table 5 and Table 4 are tested in these models of fibrotic conditions, in which it is found that they are effective in treating liver fibrosis and other fibrotic conditions.

Model Systems of Glaucoma

Testing the active siRNA of the description for treating or preventing glaucoma is preformed in rat animal model for optic nerve crush described for example in: Maeda et al., 2004 Investigative Ophthalmology and visual Science (IOVS), 45:851. Specifically, for optic nerve transection the orbital optic nerve (ON) of anesthetized rats is exposed through a supraorbital approach, the meninges severed and all axons in the ON transected by crushing with forceps for 10 seconds, 2 mm from the lamina cribrosa.

Nucleic acid molecules as disclosed herein are tested in this animal model and the results show that these siRNA compounds are useful in treating and/or preventing glaucoma.

Rat Optic Nerve Crush (ONC) Model: Intravitreal siRNA Delivery and Eye Drop Delivery

For optic nerve transsection the orbital optic nerve (ON) of anesthetized rats is exposed through a supraorbital approach, the meninges severed and all axons in the ON transected by crushing with forceps for 10 seconds, 2 mm from the lamina cribrosa.

The siRNA compounds are delivered alone or in combination in 5 uL volume (10 ug/uL) as eye drops Immediately after optic nerve crush (ONC), 20 ug/10 ul test siRNA or 10 ul PBS is administered to one or both eyes of adult Wistar rats and the levels of siRNA taken up into the dissected and snap frozen whole retinae at 5 h and 1 d, and later at 2 d, 4 d, 7 d, 14 d and 21 d post injection is determined Similar experiments are performed in order to test activity and efficacy of siRNA administered via eye drops.

Model Systems of Ischemia Reperfusion Injury Following Lung Transplantation in Rats

Lung ischemia/reperfusion injury is achieved in a rat animal model as described in Mizobuchi et al., 2004 J Heart Lung Transplantation, 23 and in Kazuhiro et al., 2001 Am. J. Respir. Cell Mol Biol, 25:26-34.

Specifically, after inducing anesthesia with isofluorane, the trachea is cannulated with a 14-gauge Teflon catheter and the rat is mechanically ventilated with rodent ventilator using 100% oxygen, at a rate of 70 breaths per minute and 2 cm H₂O of positive end-respiratory pressure. The left pulmonary artery, veins and main stem bronchus are occluded with a Castaneda clamp. During the operation, the lung is kept moist with saline and the incision is covered to minimize evaporative losses. The period of ischemia is 60 minutes long. At the end of the ischemic period the clamp is removed and the lung is allowed to ventilate and reperfuse for further 4 h, 24 h, and 5 d post induction of lung ischemia. At the end of the experiment, the lungs are gently harvested and either frozen for RNA extraction or fixed in glutaraldehyde cocktail for subsequent histological analysis.

the Bleomycin Animal Model as a Model for Idiopathic Pulmonary Fibrosis (IPF)

Testing feasibility of lung and liver delivery of vitamin A-Coatsome formulated siRNA administered by intravenous injection and intratracheal administration of siRNA-vitaminA-Coatsome complex to a healthy mice and bleomycine-treated mice

Objective: To test two administration routes for feasibility of vitamin A-Coatsome formulated siRNA delivery to normal and fibrotic mouse lungs. The main hypothesis to be tested in the current study is whether systemic administration of vitamin A-Coatsome formulated modified siRNA provides efficient uptake and cell-specific distribution in the fibrotic and normal mouse lungs. Intratracheal route of vitaminA-Coatsome formulated modified siRNA will be tested in parallel. siRNA detection and cell-specific distribution in the lungs and liver will be performed by in situ hybridization (ISH)

The Bleomycin model of pulmonary fibrosis has been well developed and characterized over the last three decades (Moeller, et al. 2008 Int J Biochem Cell Biol, 40:362-82; Chua et al., 2005 Am J Respir Cell Mol Biol 33:9-13,). Histological hallmarks, such as intra-alveolar buds, mural incorporation of collagen and obliteration of alveolar space are present in BLM-treated animals similar to IPF patients. Early studies demonstrated that C57/B1 mice were consistently prone to BLM-induced lung fibrosis, whereas Balb/C mice were inheritantly resistant. Depending on the route of administration, different fibrotic pattern develops. Intratracheal instillation of BLM results in bronchiocentric accentuated fibrosis, whereas intravenous or intraperitoneal administration induces subpleural scarring similar to human disease (Chua et al. ibid.). A mouse model of usual interstitial pneumonia (UIP) is used. This model shows a heterogenous distribution of fibroproliferation, distributed mainly subpleurally, forming similar lesions to those observed in the lungs of patients with idiopathic pulmonary fibrsosis (IPF) (Onuma, et al., 2001 J Exp Med 194:147-56, and Yamaguchi et al., 1988 Nature 336:244-46). UIP will be induced by intraperitoneal injection of bleomycin every other day for 7 days for a constant composition of subpleural fibroproliferation in the mouse lung (Swiderski et al. 1998 Am J Pathol 152: 821-28, and Shimizukawa et al., 2003 Am J Physiol Lung Cell Mol Physiol 284: L526-32,).

Vitamin A-loaded liposomes containing siRNA interact with retinol-binding protein (RBP) and provide efficient delivery to the HSC via RBP receptor. These liposomes are efficiently taken up by an RBP receptor-expressing activated myofibroblasts in the lungs of bleomycin-treated mice.

Study Design

Mice—C57 Bl male

Starting N (BLM I.P.)—40 (6 for the first pilot group, 34 for the study, taling in consideration anticipated 25% mortality)

Starting N (Total)—60

Test siRNA: SERPINHI compounds disclosed herein.

TABLE 8 Groups: BLM dose, mg/kg siRNA BW, in BLM dose, Termination N (before 0.1 ml adm. mg/kg siRNA siRNA post last siRNA No saline route BLM regime BW adm route regime siRNA adm administration) 1 0.75 I.P. dd 0, 2, 4, 6 4.5 I.V. 2 daily 2 h 4 2 0.75 I.P. dd 0, 2, 4, 6 4.5 I.V. 2 daily 24 h  4 3 0.75 I.P. dd 0, 2, 4, 6 2.25 I.T. 2 daily 2 h 4 4 0.75 I.P. dd 0, 2, 4, 6 2.25 I.T. 2 daily 24 h  4 5 intact n/a 4.5 I.V. 2 daily 2 h 4 6 intact n/a 4.5 I.V 2 daily 24 h  4 7 intact n/a 2.25 I.T 2 daily 2 h 4 8 intact n/a 2.25 I.T. 2 daily 24 h  4 9 0.75 I.P. dd 0, 2, 4, 6 n/a I.V. 2 daily 2 h 3 vehicle 10 0.75 I.P. dd 0, 2, 4, 6 n/a I/T/vehicle 2 daily 24 h  3 11 Intact n/a n/a intact n/a Any time 3

Bleomycin-Induced Pulmonary Fibrosis.

Pulmonary fibrosis of 12-wk-old female C57BL/6 mice will be induced by intraperitoneal instillation of bleomycin chlorate: 0.75 mg/kg body weight dissolved in 0.1 ml of saline every other day for 7 days, on days 0, 2, 4, and 6.

Pilot Evaluation of the Establishment of Fibrosis.

The mice (N=30) are subjected to BLM treatment in groups, to allow for a one week time interval between the first treated group (N=5) and the rest of the animals. On day 14, two mice from the first group are sacrificed and the lungs harvested for the fast HE stain and quick histopathological evaluation of fibrosis. When lung fibrosis is confirmed, the remaining rats are sorted into the groups and treated with siRNA on Day 14 after the first BLM treatment. In case that no sufficient fibrosis develops in the lungs by day 14, the remaining mice from the first treated group are sacrificed on day 21, followed by quick histopathology evaluation of fibrosis. The rest of the animals are treated with test siRNA complex starting from day 21 after the BLM treatment.

siRNA Administration.

On day 14 or day 21 after the first BLM administration (TBD during the study, based on pilot evaluation of establishment of fibrosis), the animals are group sorted, according to BW. The animals from groups 1 and 2 are administered intravenously (tail vein injection) with siRNA/vitA/Coatsome complex, at an siRNA concentration of 4.5 mg/kg BW. Intact animals of the same age (Groups 5 and 6) are treated in the same manner. BLM treated animals (Group 9) will be used as vitA-coatsome vehicle control). In 24 hours, the injection is repeated to all the animals, as above.

The BLM animals from the groups 3 and 4, and intact mice from groups 7 and 8 are anesthetized with isoflutrane and subjected to intratracheal instillation of 2.25 mg/kg BW siRNA formulated in vitA-loaded liposomes. Mice from the BLM group 10 are administered with vitA/Coatsome vehicle only. The inttratracheal instillation is repeated after 24 hours.

Study Termination.

The animals from the groups 1, 3, 5, 7, 9 are sacrificed at 2 hours after the second siRNA complex injection or instillation. The animals from the groups 2, 4, 6, 8, 10 are sacrificed at 24 hours after the second siRNA complex injection or instillation.

Upon animals sacrifice, the mice are perfused transcardially with 10% neutral buffered formalin. The lungs are inflated with 0.8-1.0 ml of 10% NBF, and the trachea ligated. The lungs are excised and fixed for 24 h in 10% NBF. The liver is harvested from each animal and fixed in 10% NBF for 24 h.

Sectioning and Evaluation.

Consequent sections are prepared from the lungs and livers. First consequent section are stained with hematoxylin and eosin for assessment of lung and liver morphology, second section are stained with Sirius Red (trichrome) to identify collagen. The third consequent sections are subjected to in situ hybridization (ISH) for detection of siRNA.

Example 22 In Vivo Anti-Pulmonary-Fibrosis Activity of siRNA-Containing VA-Bound Liposome

(1) Induction of Pulmonary Fibrosis and Administration of Drug

Male S-D rats (8 rats/group, 8 weeks old, Charles River Laboratories Japan, Inc.) were administered once with 0.45 mg bleomycin (BLM) dissolved in 0.1 mL of saline into the lung by intratracheal cannulation (MicroSprayer, Penn-Century, Inc.) under anesthesia, to produce a bleomycin pulmonary fibrosis model. With this method, a significant fibrosis occurs in the lung generally after approximately 2 weeks. The liposome formulation (1.5 mg/kg as an amount of siRNA, 1 ml/kg in volume, i.e., 200 μl for a rat of 200 g) or PBS (1 ml/kg in volume) was administered to the rats via the tail vein, starting from the 2 weeks after the bleomycin administration, for total of ten times (every other day). The rats were sacrificed at two days post last treatment, histological investigation of the lung tissue was performed (see FIG. 30). One way ANOVA and Bonferroni multi comparison test was used for the evaluation of statistically-significant difference.

The composition of the liposome was HEDC/S-104/DOPE/Cholesterol/PEG-DMPE/diVA-PEG-diVA (20:20:30:25:5:2 Molar %. Details of siRNA were as follows:

S strand: (SEQ ID NO: 2748) 5′-idAB-rG-rA-rG-rA-rC-rA-rC-rA-rU-rG-rG-rG-rU-rG- 25rC-25rU-25rA-25rU-25rA-C3-P-3′ GS strand: (SEQ ID NO: 2749) 5′-mU-rA-mU-rA-mG-rC-25rA-rC-mC-rC-mA-rU-mG-rU-mG- rU-mC-rU-mC-C3-C3-3′ wherein: rX represents ribonucleotides; mX represents 2′-O-methyl ribonucleotides; 25rX represents ribonucleotides with 2′-5′ linkages; C3 represents a 1,3-propanediol spacer; idAB represents inverted 1,2-dideoxy-D-ribose; P represents a phosphate group on the 3′-terminus. The 3′-terminus C3 is introduced by support-loaded 1,3-propanediol spacer. The 3′-terminus phosphate group (P) is introduced by the use of support-loaded diethyl sulfonyl (Pi) spacer.

(2) Histological Investigation

A part of the removed lung was formalin-fixed in accordance with a routine method, and subjected to azan staining (azocarmine, aniline blue orange G solution). As shown by the results of the azan staining in FIG. 31, in the PBS administration group (Disease), a noticeable fibrotic image characterized by enlargement of interstice due to a large quantity of blue-stained collagenous fibrils was observed, whereas in the formulation administration group (Treatment), fibrosis were apparently suppressed.

As shown by the results of histological scoring (T. Ashcroft score) in FIG. 32, in the formulation administration group (Treatment), fibrosis score was significantly decreased.

Example 23 In-Vivo Reduction of HSP47 mRNA (DMN Model)

In vivo activity of the HSP47 liposomes of Example 22 was evaluated in the short-term liver damage model (referred to as the Quick Model). In this model, the short-term liver damage induced by treatment with a hepatotoxic agent such as dimethylnitrosamine (DMN) is accompanied by the elevation of HSP47 mRNA levels. To induce these changes, male Sprague-Dawley rats were injected intraperitoneally with DMN on six consecutive days. At the end of the DMN treatment period, the animals were randomized to groups based upon individual animal body weight. The liposome sample was administered as a single IV dose (0.375 or 0.75 mg/kg, reflecting the siRNA dose) one hour after the last injection of DMN. One day later, liver lobes were excised and both HSP47 and GAPDH mRNA levels were determined by quantitative RT-PCR (TaqMan) assay. HSP47 mRNA levels were normalized to GAPDH levels. As shown in FIG. 33, robust and dose-dependent mRNA reduction for HSP47 was detected in liver. After a single dose of 0.75 mg/kg of ND-L02-0101, 80% reduction of HSP47 mRNA was observed. Even at the lower dose of 0.375 mg/kg, significant knockdown of 40% was observed.

The contents of the articles, patents, and patent applications, and all other documents and electronically available information mentioned or cited herein, are hereby incorporated by reference in their entirety to the same extent as if each individual publication was specifically and individually indicated to be incorporated by reference.

Applicants reserve the right to physically incorporate into this application any and all materials and information from any such articles, patents, patent applications, or other physical and electronic documents.

It will be readily apparent to one skilled in the art that varying substitutions and modifications can be made to the description disclosed herein without departing from the scope and spirit of the description. Thus, such additional embodiments are within the scope of the present description and the following claims. The present description teaches one skilled in the art to test various combinations and/or substitutions of chemical modifications described herein toward generating nucleic acid constructs with improved activity for mediating RNAi activity. Such improved activity can include improved stability, improved bioavailability, and/or improved activation of cellular responses mediating RNAi. Therefore, the specific embodiments described herein are not limiting and one skilled in the art can readily appreciate that specific combinations of the modifications described herein can be tested without undue experimentation toward identifying nucleic acid molecules with improved RNAi activity.

The descriptions illustratively described herein may suitably be practiced in the absence of any element or elements, limitation or limitations, not specifically disclosed herein. Thus, for example, the terms “a” and “an” and “the” and similar referents in the context of describing the description (especially in the context of the following claims) are to be construed to cover both the singular and the plural, unless otherwise indicated herein or clearly contradicted by context. The terms “comprising”, “having,” “including,” containing”, etc. shall be read expansively and without limitation (e.g., meaning “including, but not limited to,”). Recitation of ranges of values herein are merely intended to serve as a shorthand method of referring individually to each separate value falling within the range, unless otherwise indicated herein, and each separate value is incorporated into the specification as if it were individually recited herein. All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g., “such as”) provided herein, is intended merely to better illuminate the description and does not pose a limitation on the scope of the description unless otherwise claimed. No language in the specification should be construed as indicating any non-claimed element as essential to the practice of the description. Additionally, the terms and expressions employed herein have been used as terms of description and not of limitation, and there is no intention in the use of such terms and expressions of excluding any equivalents of the features shown and described or portions thereof, but it is recognized that various modifications are possible within the scope of the description claimed. Thus, it should be understood that although the present description has been specifically disclosed by preferred embodiments and optional features, modification and variation of the descriptions embodied therein herein disclosed may be resorted to by those skilled in the art, and that such modifications and variations are considered to be within the scope of this description.

The description has been described broadly and generically herein. Each of the narrower species and subgeneric groupings falling within the generic disclosure also form part of the description. This includes the generic description of the description with a proviso or negative limitation removing any subject matter from the genus, regardless of whether or not the excised material is specifically recited herein. Other embodiments are within the following claims. In addition, where features or aspects of the description are described in terms of Markush groups, those skilled in the art will recognize that the description is also thereby described in terms of any individual member or subgroup of members of the Markush group. 

What is claimed:
 1. A pharmaceutical composition comprising a drug carrier and a double-stranded nucleic acid molecule, wherein the drug carrier comprises a liposome and a stellate cell-specific amount of diVA-PEG-diVA, and wherein the double-stranded nucleic acid molecule comprises the structure (A2) set forth below: (A2) 5′ N1-(N)x - Z 3′ (antisense strand) 3′ Z′-N2-(N′)y -z″ 5′ (sense strand)

wherein each of N2, N and N′ is an unmodified or modified ribonucleotide, or an unconventional moiety; wherein each of (N)x and (N′)y is an oligonucleotide in which each consecutive N or N′ is joined to the adjacent N or N′ by a covalent bond; wherein each of x and y is independently an integer between 17 and 39; wherein the sequence of (N′)y has complementarity to the sequence of (N)x and (N)x has complementarity to a consecutive sequence in a target RNA; wherein N1 is covalently bound to (N)x and is mismatched to the target RNA or is a complementary DNA moiety to the target RNA; wherein N1 is a moiety selected from the group consisting of natural or modified uridine, deoxyribouridine, ribothymidine, deoxyribothymidine, adenosine or deoxyadenosine; wherein z″ may be present or absent, but if present is a capping moiety covalently attached at the 5′-terminus of N2—(N′)y; wherein each of Z and Z′ is independently present or absent, but if present is independently 1-5 consecutive nucleotides, consecutive non-nucleotide moieties or a combination thereof covalently attached at the 3′-terminus of the strand in which it is present; and wherein (N)x comprises an antisense sequence to the mRNA coding sequence for human hsp47 exemplified by SEQ ID NO:1.
 2. The pharmaceutical composition according to claim 1, wherein the diVA-PEG-diVA is at least partially exposed on the exterior of the drug carrier before the drug carrier reaches the stellate cell.
 3. The pharmaceutical composition according to claim 1, wherein the drug carrier is selected from the group consisting of a polymer micelle, a liposome, an emulsion, a microsphere, and a nanosphere.
 4. The pharmaceutical composition according to claim 3, wherein the drug carrier is a lipid vesicle comprising a bilayer of lipid molecules.
 5. The pharmaceutical composition according to claim 4, wherein the diVA-PEG-diVA is 0.2 wt % to 20 wt % of the lipid molecules.
 6. The pharmaceutical composition according to claim 1, wherein the double-stranded nucleic acid molecule is exposed on the exterior surface of the lipid vesicle.
 7. The pharmaceutical composition according to claim 1, wherein the double-stranded nucleic acid molecule is encapsulated by the lipid vesicle.
 8. The pharmaceutical composition according to claim 7, wherein the double-stranded nucleic acid molecule is resistant to nucleases.
 9. The pharmaceutical composition of claim 1, wherein one strand of the double-stranded nucleic acid molecule comprises SEQ ID NO:127 and the second strand comprises SEQ ID NO:60.
 10. The pharmaceutical composition according to claim 9, wherein the antisense strand comprises SEQ ID NO:127, and the sense strand comprises SEQ ID NO:60.
 11. The pharmaceutical composition according to claim 10, wherein the antisense strand further comprises a 2′-O-methyl modified ribonucleotide; a 2′-5′-ribonucleotide in at least one of positions 1, 5, 6, or 7; and a non-nucleotide moiety covalently attached to the 3′-terminus; and wherein the sense strand further comprises at least one 2′-5′-ribonucleotide or 2′-O-methyl modified ribonucleotide; a non-nucleotide moiety covalently attached at the 3′-terminus; and a cap moiety covalently attached at the 5′-terminus.
 12. The pharmaceutical composition according to claim 10, wherein the antisense strand further comprises 2-′O-methyl modified ribonucleotides at positions 3, 5, 9, 11, 13, 15, 17, and 19; a 2′-5′-ribonucleotide in position 7; and a non-nucleotide moiety covalently attached at the 3′-terminus; and wherein the sense strand further comprises five consecutive 2′-5′-ribonucleotides in the 3′-terminal positions 15, 16, 17, 18, and 19; a non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic moiety covalently attached at the 5′-terminus.
 13. The pharmaceutical composition according to claim 10, wherein the antisense strand further comprises 2′-O-methyl modified ribonucleotides at positions 1, 3, 5, 9, 11, 13, 15, 17, and 19; and a C3C3 non-nucleotide moiety covalently attached at the 3′-terminus; and wherein the sense strand further comprises a C3Pi non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic moiety covalently attached at the 5′-terminus.
 14. The pharmaceutical composition according to claim 10, wherein the antisense strand further comprises 2′-O-methyl modified ribonucleotides at positions 1, 3, 5, 9, 11, 13, 15, 17, and 19; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH non-nucleotide moiety covalently attached at the 3′-terminus; and wherein the sense strand further comprises five consecutive 2′-5′-ribonucleotides in the 3′-terminal positions 15, 16, 17, 18, and 19; a C3Pi non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic moiety covalently attached at the 5′-terminus.
 15. The pharmaceutical composition according to claim 10, wherein the antisense strand further comprises 2-′O-methyl modified ribonucleotides in positions 1, 3, 5, 9, 11, 13, 15, 17, and 19; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH non-nucleotide moiety covalently attached at the 3′-terminus; and wherein the sense strand further comprises 2′-O-methyl modified ribonucleotides in positions 7, 13, 16 and 18; a 2′-5′-ribonucleotide at position 9; a C3 non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic moiety covalently attached at the 5′-terminus.
 16. The pharmaceutical composition of claim 1, wherein one strand of the double-stranded nucleic acid molecule comprises SEQ ID NO:98 and the second strand comprises SEQ ID NO:165.
 17. The pharmaceutical composition according to claim 16, wherein the sense strand comprises SEQ ID NO:98 and the antisense strand comprises SEQ ID NO:165.
 18. The pharmaceutical composition according to claim 17, wherein the sense strand further comprises 2′-5′-ribonucleotides in positions at or near the 3′-terminus; a non-nucleotide moiety covalently attached at the 3′-terminus; and a cap moiety covalently attached at the 5′-terminus; and the antisense strand further comprises a 2′-O-methyl modified ribonucleotide; a 2′-5′-ribonucleotide in at least one of positions 5, 6 or 7; and a non-nucleotide moiety covalently attached at the 3′-terminus.
 19. The pharmaceutical composition according to claim 17, wherein the sense strand further comprises 2′-5′-ribonucleotides in positions 15, 16, 17, 18, and 19; a C3-OH 3′ moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and the antisense strand further comprises 2′-O-methyl modified ribonucleotides in positions 2, 4, 6, 8, 11, 13, 15, 17, and 19; a 2′-5′-ribonucleotide in position 7; and a C3Pi-C3OH moiety covalently attached at the 3′-terminus.
 20. The pharmaceutical composition of claim 1, wherein one strand of the double-stranded nucleic acid molecule comprises SEQ ID NO:101 and the second strand comprises SEQ ID NO:168.
 21. The pharmaceutical composition according to claim 20, wherein the sense strand comprises SEQ ID NO:101 and the antisense strand comprises SEQ ID NO:168.
 22. The pharmaceutical composition according to claim 20, wherein the sense strand further comprises 2′-O-methyl modified pyrimidine ribonucleotides; an optional 2′-5′-ribonucleotide in one of position 9 or 10; a non-nucleotide moiety covalently attached at the 3′-terminus; and a cap moiety covalently attached at the 5′-terminus; and the wherein antisense strand further comprises a 2′-O-methyl modified ribonucleotide; a 2′-5′-ribonucleotide in at least one of positions 5, 6, or 7; and a non-nucleotide moiety covalently attached at the 3′-terminus.
 23. The pharmaceutical composition according to claim 20, wherein the sense strand further comprises 2′-O-methyl modified ribonucleotides in positions 4, 11, 13, and 17; a 2′-5′-ribonucleotide in position 9; a C3OH non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and wherein the antisense strand further comprises 2′-O-methyl modified ribonucleotides in positions 1, 4, 8, 11 and 15; a 2′-5′-ribonucleotide in position 6; and a C3Pi-C3OH moiety covalently attached at the 3′-terminus.
 24. The pharmaceutical composition according to claim 20, wherein sense strand further comprises 2′-O-methyl modified ribonucleotides in positions 4, 11, 13, and 17; a C3OH non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and wherein the antisense strand further comprises 2′-O-methyl modified ribonucleotides in positions 1, 4, 8, 13 and 15; a 2′-5′-ribonucleotide in position 6; and a C3Pi-C3OH moiety covalently attached at the 3′-terminus.
 25. The pharmaceutical composition according to claim 20, wherein the sense strand further comprises 2′-O-methyl modified ribonucleotides in positions 2, 4, 11, 13, and 17; a C3OH non-nucleotide moiety covalently attached at the 3′-terminus; and an inverted abasic deoxyribonucleotide moiety covalently attached at the 5′-terminus; and wherein the antisense strand further comprises 2′-O-methyl modified ribonucleotides in positions 1, 4, 8, 11 and 15; a 2′-5′-ribonucleotide in position 6; and a C3Pi-C3OH non-nucleotide moiety covalently attached at the 3′-terminus.
 26. The pharmaceutical composition according to claim 1, wherein the double-stranded nucleic acid molecule reduces hsp47 expression in the stellate cell.
 27. A method for treating a fibrotic disease in a subject in need thereof, the method comprising administering an effective amount of a pharmaceutical composition of claim 1 to the subject.
 28. The method according to claim 27, wherein the pharmaceutical formulation is parenterally administered.
 29. The method of claim 28, wherein the fibrotic disease is liver fibrosis.
 30. The method of claim 29, wherein liver fibrosis consists of non-alcoholic steatohepatitis; hepatitis; hepatic fibrosis; chronic hepatitis C virus infection; hepatic cirrhosis; chronic hepatic damage; or liver cancer.
 31. The method of claim 27, wherein the fibrotic disease is pulmonary fibrosis.
 32. The method of claim 31, wherein the pulmonary fibrosis consists of pulmonary fibrosis including idiopathic pulmonary fibrosis, interstitial lung disease, radiation pneumonitis leading to pulmonary fibrosis; or fibrotic lung disease.
 33. The method of claim 27, wherein the fibrotic disease is kidney fibrosis.
 34. The method of claim 33, wherein the kidney fibrosis is chronic renal failure or diabetic nephropathy.
 35. The method of claim 27, wherein the fibrotic disease is peritoneal fibrosis.
 36. The method of claim 35, wherein the peritoneal fibrosis is peritoneal sclerosis associated with continual ambulatory peritoneal dialysis.
 37. The method of claim 27, wherein the fibrotic disease is pancreatic fibrosis.
 38. The method of claim 37, wherein the pancreatic fibrosis consists of pancreatitis, pancreatic fibrosis, or pancreatic cancer.
 39. The method of claim 27, wherein the fibrotic disease is cardiac fibrosis.
 40. The method of claim 39, wherein the cardiac fibrosis consists of myocardial fibrosis cardiomyopathy, atherosclerosis, endomyocardial fibrosis, atrial fibrillation, or scarring post-myocardial infarction.
 41. The method of claim 27, wherein the fibrotic disease is intestinal fibrosis
 42. The method of claim 27, wherein the fibrotic disease is fibrosis of the eye.
 43. The method of claim 42, wherein the fibrosis of the eye consists of ocular scarring including proliferative vitreoretinopathy (PVR) or scarring resulting from surgery to treat cataract or macular degeneration, glaucoma, Grave's ophthalmopathy; ocular cicatricial pemphigoid, or drug induced ergotism.
 44. The method of claim 27, wherein the fibrotic disease is skin fibrosis.
 45. The method of claim 44, wherein the skin fibrosis consists of skin fibrosis including scleroderma, keloids and hypertrophic scars scleroderma; psoriasis; or Kaposi's sarcoma.
 46. The method of claim 27, wherein the fibrotic disease is fibrosis of bone.
 47. The method of claim 46, wherein the fibrosis of bone consists of myelofibrosis; myleoid leukemia; acute myelogenous leukemia; myelodysplastic syndrome; lymphangiolyomyositosis (LAM), chronic graft vs. host disease, polycythemia vera, essential thrombocythemia, or myeloproferative syndrome.
 48. The method of claim 27, wherein the fibrotic disease is inflammatory bowel disease of variable etiology.
 49. The method of claim 27, wherein the fibrotic disease is glioblastoma in Li-Fraumeni syndrome or sporadic glioblastoma.
 50. The method of claim 27, wherein the fibrotic disease is gynecological cancer.
 51. The method of claim 27, wherein the fibrotic disease is Hansen's disease.
 52. The method of claim 27, wherein the fibrotic disease is collagenous colitis.
 53. The method of claim 27, wherein the fibrotic disease is fibrillogenesis.
 54. The method of claim 27, wherein the fibrotic disease is fibrosis of vocal cords.
 55. The method of claim 54, wherein the fibrosis of vocal cords consists of vocal cord scarring, vocal cord mucosal fibrosis, or laryngeal fibrosis. 